Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_18.17.12 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "1f7f874c1a30a72c08d5d6b05a021406",
"name": "M1_20170616_1605_dsRNA_O2_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "16:17:34"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8145 (max 8146) in '8145'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.8824
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.19282e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 33 mean_circ: 0.937814
Ran for 1550 frames Cell Events: 46 mean_circ: 0.937333
Ran for 2050 frames Cell Events: 59 mean_circ: 0.941983
Ran for 2550 frames Cell Events: 70 mean_circ: 0.944345
Ran for 3050 frames Cell Events: 91 mean_circ: 0.943653
Ran for 3550 frames Cell Events: 107 mean_circ: 0.943835
Ran for 4050 frames Cell Events: 123 mean_circ: 0.94043
Ran for 5050 frames Cell Events: 165 mean_circ: 0.93825
Ran for 5550 frames Cell Events: 185 mean_circ: 0.938569
Ran for 6050 frames Cell Events: 205 mean_circ: 0.935349
Ran for 7550 frames Cell Events: 248 mean_circ: 0.934945
Ran for 8050 frames Cell Events: 263 mean_circ: 0.935159
Ran for 8550 frames Cell Events: 277 mean_circ: 0.935017
Ran for 9050 frames Cell Events: 285 mean_circ: 0.93557
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Ran for 10550 frames Cell Events: 341 mean_circ: 0.935842
Ran for 12050 frames Cell Events: 432 mean_circ: 0.937364
Ran for 14050 frames Cell Events: 510 mean_circ: 0.937335
Ran for 14550 frames Cell Events: 537 mean_circ: 0.937752
Ran for 15050 frames Cell Events: 558 mean_circ: 0.937799
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Ran for 16550 frames Cell Events: 611 mean_circ: 0.937841
Ran for 17050 frames Cell Events: 619 mean_circ: 0.937809
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Ran for 19050 frames Cell Events: 722 mean_circ: 0.936682
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Ran for 24550 frames Cell Events: 919 mean_circ: 0.936336
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Ran for 25550 frames Cell Events: 952 mean_circ: 0.936572
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Ran for 35050 frames Cell Events: 1279 mean_circ: 0.936464
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Ran for 61550 frames Cell Events: 2319 mean_circ: 0.936437
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Ran for 63050 frames Cell Events: 2372 mean_circ: 0.936331
Ran for 63550 frames Cell Events: 2391 mean_circ: 0.936301
Ran for 64550 frames Cell Events: 2426 mean_circ: 0.936401
Ran for 65050 frames Cell Events: 2446 mean_circ: 0.93626
Ran for 65550 frames Cell Events: 2482 mean_circ: 0.936189
Ran for 67550 frames Cell Events: 2587 mean_circ: 0.936343
Ran for 69550 frames Cell Events: 2687 mean_circ: 0.936246
Ran for 70050 frames Cell Events: 2703 mean_circ: 0.936187
Ran for 70550 frames Cell Events: 2721 mean_circ: 0.93615
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Ran for 71550 frames Cell Events: 2743 mean_circ: 0.936212
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Ran for 72550 frames Cell Events: 2794 mean_circ: 0.936306
Ran for 73050 frames Cell Events: 2823 mean_circ: 0.936376
Ran for 73550 frames Cell Events: 2836 mean_circ: 0.93646
Ran for 74050 frames Cell Events: 2867 mean_circ: 0.936448
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Ran for 76050 frames Cell Events: 2967 mean_circ: 0.936222
Ran for 76550 frames Cell Events: 2982 mean_circ: 0.936323
Ran for 77050 frames Cell Events: 3003 mean_circ: 0.936342
Ran for 77550 frames Cell Events: 3019 mean_circ: 0.936453
Ran for 79050 frames Cell Events: 3089 mean_circ: 0.936432
Ran for 80050 frames Cell Events: 3134 mean_circ: 0.936449
Ran for 80550 frames Cell Events: 3150 mean_circ: 0.936394
Ran for 81050 frames Cell Events: 3176 mean_circ: 0.936427
Ran for 81550 frames Cell Events: 3194 mean_circ: 0.936436
Ran for 82050 frames Cell Events: 3216 mean_circ: 0.936411
Ran for 82550 frames Cell Events: 3229 mean_circ: 0.936357
Ran for 83550 frames Cell Events: 3274 mean_circ: 0.936228
Ran for 84050 frames Cell Events: 3291 mean_circ: 0.936176
Ran for 85550 frames Cell Events: 3322 mean_circ: 0.936176
Ran for 86550 frames Cell Events: 3352 mean_circ: 0.936334
Ran for 87050 frames Cell Events: 3371 mean_circ: 0.936357
Ran for 87550 frames Cell Events: 3384 mean_circ: 0.936247
Ran for 88050 frames Cell Events: 3399 mean_circ: 0.936292
Ran for 88550 frames Cell Events: 3421 mean_circ: 0.936086
Ran for 89550 frames Cell Events: 3461 mean_circ: 0.936055
Ran for 91550 frames Cell Events: 3549 mean_circ: 0.935983
Ran for 92550 frames Cell Events: 3586 mean_circ: 0.935999
Ran for 93050 frames Cell Events: 3598 mean_circ: 0.936071
Ran for 93550 frames Cell Events: 3626 mean_circ: 0.936003
Ran for 94050 frames Cell Events: 3652 mean_circ: 0.936007
Ran for 95050 frames Cell Events: 3718 mean_circ: 0.935665
Ran for 95550 frames Cell Events: 3750 mean_circ: 0.935567
Ran for 96050 frames Cell Events: 3773 mean_circ: 0.935513
Ran for 97550 frames Cell Events: 3798 mean_circ: 0.935608
Ran for 98050 frames Cell Events: 3826 mean_circ: 0.935654
Ran for 99050 frames Cell Events: 3874 mean_circ: 0.935567
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Ran for 100050 frames Cell Events: 3933 mean_circ: 0.935522
Ran for 100550 frames Cell Events: 3952 mean_circ: 0.93555
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Ran for 102550 frames Cell Events: 4007 mean_circ: 0.935407
Ran for 103050 frames Cell Events: 4043 mean_circ: 0.935342
Ran for 103550 frames Cell Events: 4069 mean_circ: 0.935303
Ran for 104550 frames Cell Events: 4108 mean_circ: 0.935221
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Ran for 105550 frames Cell Events: 4139 mean_circ: 0.93522
Ran for 106050 frames Cell Events: 4166 mean_circ: 0.935116
Ran for 106550 frames Cell Events: 4183 mean_circ: 0.93512
Ran for 107050 frames Cell Events: 4205 mean_circ: 0.935145
Ran for 108050 frames Cell Events: 4248 mean_circ: 0.935156
Ran for 109050 frames Cell Events: 4293 mean_circ: 0.935227
Ran for 109550 frames Cell Events: 4305 mean_circ: 0.935304
Ran for 110050 frames Cell Events: 4335 mean_circ: 0.935258
Ran for 111050 frames Cell Events: 4364 mean_circ: 0.935268
Ran for 111550 frames Cell Events: 4385 mean_circ: 0.935176
Ran for 113050 frames Cell Events: 4464 mean_circ: 0.935207
Ran for 113550 frames Cell Events: 4479 mean_circ: 0.935259
Ran for 114050 frames Cell Events: 4498 mean_circ: 0.935233
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Ran for 115050 frames Cell Events: 4526 mean_circ: 0.935234
Ran for 115550 frames Cell Events: 4541 mean_circ: 0.9352
Ran for 116050 frames Cell Events: 4569 mean_circ: 0.935236
Ran for 117050 frames Cell Events: 4632 mean_circ: 0.935322
Ran for 117550 frames Cell Events: 4643 mean_circ: 0.935323
Ran for 118050 frames Cell Events: 4658 mean_circ: 0.935314
Ran for 118550 frames Cell Events: 4675 mean_circ: 0.935362
Ran for 119050 frames Cell Events: 4686 mean_circ: 0.935387
Ran for 119550 frames Cell Events: 4704 mean_circ: 0.935364
Ran for 120050 frames Cell Events: 4725 mean_circ: 0.935365
Ran for 120550 frames Cell Events: 4734 mean_circ: 0.935434
Ran for 122050 frames Cell Events: 4804 mean_circ: 0.935499
Ran for 122550 frames Cell Events: 4828 mean_circ: 0.935525
Ran for 123050 frames Cell Events: 4864 mean_circ: 0.935384
Ran for 123550 frames Cell Events: 4881 mean_circ: 0.935431
Ran for 124050 frames Cell Events: 4898 mean_circ: 0.935369
Ran for 124550 frames Cell Events: 4927 mean_circ: 0.935266
Ran for 125050 frames Cell Events: 4953 mean_circ: 0.935245
Ran for 125550 frames Cell Events: 4976 mean_circ: 0.935245
Ran for 126050 frames Cell Events: 5000 mean_circ: 0.935317
Ran for 128050 frames Cell Events: 5088 mean_circ: 0.935236
Ran for 129550 frames Cell Events: 5188 mean_circ: 0.935186
Ran for 130050 frames Cell Events: 5213 mean_circ: 0.93521
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Ran for 131050 frames Cell Events: 5244 mean_circ: 0.935256
Ran for 131550 frames Cell Events: 5257 mean_circ: 0.935301
Ran for 132550 frames Cell Events: 5287 mean_circ: 0.935332
Ran for 133050 frames Cell Events: 5311 mean_circ: 0.935335
Ran for 133550 frames Cell Events: 5325 mean_circ: 0.935334
Ran for 134050 frames Cell Events: 5344 mean_circ: 0.935357
Ran for 134550 frames Cell Events: 5371 mean_circ: 0.935383
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Ran for 141550 frames Cell Events: 5740 mean_circ: 0.935246
Ran for 142050 frames Cell Events: 5764 mean_circ: 0.935366
Ran for 142550 frames Cell Events: 5793 mean_circ: 0.935329
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Ran for 146050 frames Cell Events: 5946 mean_circ: 0.935265
Ran for 146550 frames Cell Events: 5962 mean_circ: 0.9353
Ran for 147050 frames Cell Events: 5985 mean_circ: 0.935366
Ran for 147550 frames Cell Events: 6000 mean_circ: 0.935376
Ran for 148050 frames Cell Events: 6021 mean_circ: 0.935352
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Ran for 152550 frames Cell Events: 6250 mean_circ: 0.93524
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Ran for 154050 frames Cell Events: 6325 mean_circ: 0.935244
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Ran for 162050 frames Cell Events: 6691 mean_circ: 0.93503
Ran for 163050 frames Cell Events: 6723 mean_circ: 0.935028
Ran for 163550 frames Cell Events: 6749 mean_circ: 0.935043
Ran for 164550 frames Cell Events: 6794 mean_circ: 0.934956
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Ran for 167050 frames Cell Events: 6962 mean_circ: 0.934827
Ran for 169050 frames Cell Events: 7081 mean_circ: 0.934876
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Ran for 171550 frames Cell Events: 7138 mean_circ: 0.934846
Ran for 172050 frames Cell Events: 7158 mean_circ: 0.934852
Ran for 173050 frames Cell Events: 7213 mean_circ: 0.93481
Ran for 173550 frames Cell Events: 7231 mean_circ: 0.93482
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Ran for 177050 frames Cell Events: 7344 mean_circ: 0.934887
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Ran for 181550 frames Cell Events: 7527 mean_circ: 0.934802
Ran for 182050 frames Cell Events: 7548 mean_circ: 0.934821
Ran for 183550 frames Cell Events: 7584 mean_circ: 0.93479
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Ran for 185550 frames Cell Events: 7668 mean_circ: 0.934715
Ran for 186050 frames Cell Events: 7691 mean_circ: 0.934696
Ran for 187550 frames Cell Events: 7768 mean_circ: 0.934644
Ran for 188050 frames Cell Events: 7779 mean_circ: 0.934633
Ran for 189550 frames Cell Events: 7852 mean_circ: 0.934648
Ran for 190050 frames Cell Events: 7868 mean_circ: 0.93468
Ran for 193050 frames Cell Events: 8041 mean_circ: 0.934747
Ran for 193550 frames Cell Events: 8063 mean_circ: 0.934763
Ran for 194050 frames Cell Events: 8075 mean_circ: 0.934757
Ran for 194550 frames Cell Events: 8096 mean_circ: 0.93475
Ran for 195050 frames Cell Events: 8114 mean_circ: 0.93473
Ran for 195550 frames Cell Events: 8131 mean_circ: 0.934732



End this measurment..



Stopping time: 2.1938e+008
****************************************
Measurement finished

Mean Circularitiy: 0.93469
Mean Area: 602.288
Mean FL-1 intensity: 3196.11
Mean FL-2 intensity: 4476.11
Mean FL-3 intensity: 0

frames processed: 193367
Total Time: 97979.2ms
Number of Runs (new bg img) 0
Frames collected: 195811
Skipped frames: 32016
Rescued Frames: 2852557
Samples DAQ: 48954779
Samples written: 97905284
Samples per Channel: 48952642
FPS: 1998.5

Samples per second: 499623
Expected samples/s: 500000

Events: 8146
Events per second: 83.1401
jumped back (PMT): 14
#Frames read, frequency
# 0 frames :272489
# 1 frames :105077
# 2 frames :3727
# 3 frames :1307
# 4 frames :529
# 5 frames :210
# 6 frames :2962
# 7 frames :1483
# 8 frames :1089
# 9 frames :503
# 10 frames :361
# 11 frames :368
# 12 frames :293
# 13 frames :170
# 14 frames :227
# 15 frames :156
# 16 frames :94
# 17 frames :93
# 18 frames :51
# 19 frames :48
# 20 frames :42
# 21 frames :45
# 22 frames :49
# 23 frames :24
# 24 frames :27
# 25 frames :20
# 26 frames :18
# 27 frames :13
# 28 frames :13
# 29 frames :11
# 30 frames :10
# 31 frames :12
# 32 frames :6
# 33 frames :2
# 34 frames :6
# 35 frames :7
# 36 frames :5
# 37 frames :4
# 38 frames :3
# 39 frames :1
# 40 frames :2
# 41 frames :1
# 42 frames :4
# 44 frames :1
# 45 frames :1
# 46 frames :2
# 47 frames :1
# 48 frames :1
# 50 frames :1
# 52 frames :1
# 58 frames :1
# 71 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.8824
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0