Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_18.19.01 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "c22fdf5ad39fa65660cde543b168a87d",
"name": "M1_20170616_1633_dsRNA_o7_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "16:19:22"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8220 (max 8221) in '8220'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.7223
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.20986e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 28 mean_circ: 0.93675
Ran for 1050 frames Cell Events: 62 mean_circ: 0.930959
Ran for 1550 frames Cell Events: 86 mean_circ: 0.929701
Ran for 2050 frames Cell Events: 115 mean_circ: 0.931421
Ran for 2550 frames Cell Events: 153 mean_circ: 0.930768
Ran for 4050 frames Cell Events: 226 mean_circ: 0.933992
Ran for 4550 frames Cell Events: 243 mean_circ: 0.933156
Ran for 5050 frames Cell Events: 277 mean_circ: 0.932849
Ran for 5550 frames Cell Events: 302 mean_circ: 0.933336
Ran for 6550 frames Cell Events: 372 mean_circ: 0.934051
Ran for 7050 frames Cell Events: 397 mean_circ: 0.934745
Ran for 8050 frames Cell Events: 445 mean_circ: 0.934798
Ran for 9550 frames Cell Events: 518 mean_circ: 0.935931
Ran for 10550 frames Cell Events: 565 mean_circ: 0.935768
Ran for 12550 frames Cell Events: 706 mean_circ: 0.936735
Ran for 13050 frames Cell Events: 740 mean_circ: 0.936735
Ran for 13550 frames Cell Events: 774 mean_circ: 0.937245
Ran for 15050 frames Cell Events: 864 mean_circ: 0.93645
Ran for 15550 frames Cell Events: 897 mean_circ: 0.936298
Ran for 16550 frames Cell Events: 959 mean_circ: 0.936999
Ran for 19050 frames Cell Events: 1089 mean_circ: 0.937732
Ran for 19550 frames Cell Events: 1123 mean_circ: 0.937263
Ran for 20050 frames Cell Events: 1165 mean_circ: 0.937192
Ran for 20550 frames Cell Events: 1191 mean_circ: 0.937208
Ran for 21050 frames Cell Events: 1212 mean_circ: 0.937281
Ran for 22050 frames Cell Events: 1272 mean_circ: 0.93719
Ran for 26050 frames Cell Events: 1539 mean_circ: 0.937594
Ran for 26550 frames Cell Events: 1560 mean_circ: 0.93775
Ran for 27550 frames Cell Events: 1600 mean_circ: 0.93758
Ran for 28550 frames Cell Events: 1684 mean_circ: 0.93741
Ran for 29550 frames Cell Events: 1726 mean_circ: 0.937415
Ran for 30550 frames Cell Events: 1802 mean_circ: 0.937108
Ran for 32050 frames Cell Events: 1873 mean_circ: 0.937167
Ran for 32550 frames Cell Events: 1899 mean_circ: 0.937074
Ran for 33050 frames Cell Events: 1927 mean_circ: 0.93685
Ran for 33550 frames Cell Events: 1950 mean_circ: 0.937011
Ran for 34550 frames Cell Events: 1990 mean_circ: 0.936815
Ran for 37050 frames Cell Events: 2131 mean_circ: 0.936764
Ran for 38050 frames Cell Events: 2187 mean_circ: 0.937007
Ran for 41050 frames Cell Events: 2371 mean_circ: 0.937158
Ran for 43050 frames Cell Events: 2504 mean_circ: 0.937584
Ran for 43550 frames Cell Events: 2535 mean_circ: 0.937469
Ran for 44550 frames Cell Events: 2592 mean_circ: 0.937509
Ran for 45050 frames Cell Events: 2609 mean_circ: 0.937582
Ran for 45550 frames Cell Events: 2634 mean_circ: 0.937581
Ran for 46550 frames Cell Events: 2692 mean_circ: 0.93764
Ran for 47550 frames Cell Events: 2752 mean_circ: 0.937809
Ran for 48050 frames Cell Events: 2775 mean_circ: 0.937752
Ran for 51550 frames Cell Events: 3005 mean_circ: 0.937612
Ran for 52050 frames Cell Events: 3040 mean_circ: 0.937623
Ran for 53050 frames Cell Events: 3119 mean_circ: 0.937599
Ran for 57550 frames Cell Events: 3449 mean_circ: 0.938042
Ran for 58550 frames Cell Events: 3516 mean_circ: 0.937916
Ran for 60050 frames Cell Events: 3627 mean_circ: 0.937916
Ran for 61050 frames Cell Events: 3697 mean_circ: 0.937928
Ran for 63050 frames Cell Events: 3839 mean_circ: 0.937965
Ran for 63550 frames Cell Events: 3869 mean_circ: 0.937906
Ran for 64550 frames Cell Events: 3923 mean_circ: 0.93789
Ran for 65050 frames Cell Events: 3953 mean_circ: 0.937992
Ran for 66050 frames Cell Events: 4020 mean_circ: 0.938057
Ran for 66550 frames Cell Events: 4051 mean_circ: 0.938058
Ran for 67050 frames Cell Events: 4091 mean_circ: 0.938058
Ran for 68050 frames Cell Events: 4145 mean_circ: 0.938063
Ran for 71050 frames Cell Events: 4365 mean_circ: 0.93769
Ran for 73550 frames Cell Events: 4512 mean_circ: 0.937634
Ran for 74050 frames Cell Events: 4536 mean_circ: 0.937534
Ran for 74550 frames Cell Events: 4556 mean_circ: 0.937576
Ran for 75050 frames Cell Events: 4592 mean_circ: 0.937634
Ran for 76050 frames Cell Events: 4640 mean_circ: 0.937711
Ran for 78050 frames Cell Events: 4737 mean_circ: 0.937783
Ran for 79050 frames Cell Events: 4795 mean_circ: 0.937805
Ran for 80050 frames Cell Events: 4844 mean_circ: 0.937946
Ran for 81050 frames Cell Events: 4905 mean_circ: 0.937906
Ran for 81550 frames Cell Events: 4926 mean_circ: 0.937905
Ran for 82050 frames Cell Events: 4954 mean_circ: 0.937815
Ran for 82550 frames Cell Events: 4979 mean_circ: 0.937843
Ran for 83050 frames Cell Events: 5011 mean_circ: 0.937843
Ran for 83550 frames Cell Events: 5032 mean_circ: 0.937869
Ran for 84050 frames Cell Events: 5066 mean_circ: 0.937893
Ran for 86550 frames Cell Events: 5238 mean_circ: 0.937935
Ran for 87050 frames Cell Events: 5267 mean_circ: 0.937935
Ran for 88050 frames Cell Events: 5328 mean_circ: 0.937839
Ran for 92050 frames Cell Events: 5574 mean_circ: 0.93775
Ran for 93550 frames Cell Events: 5668 mean_circ: 0.937867
Ran for 97050 frames Cell Events: 5889 mean_circ: 0.93788
Ran for 100050 frames Cell Events: 6071 mean_circ: 0.937716
Ran for 101050 frames Cell Events: 6131 mean_circ: 0.937749
Ran for 102050 frames Cell Events: 6199 mean_circ: 0.937758
Ran for 103550 frames Cell Events: 6293 mean_circ: 0.937778
Ran for 104050 frames Cell Events: 6316 mean_circ: 0.937746
Ran for 104550 frames Cell Events: 6335 mean_circ: 0.937766
Ran for 105550 frames Cell Events: 6402 mean_circ: 0.937779
Ran for 108550 frames Cell Events: 6587 mean_circ: 0.937734
Ran for 109050 frames Cell Events: 6632 mean_circ: 0.937686
Ran for 109550 frames Cell Events: 6647 mean_circ: 0.937692
Ran for 110550 frames Cell Events: 6719 mean_circ: 0.937656
Ran for 111550 frames Cell Events: 6772 mean_circ: 0.937608
Ran for 112050 frames Cell Events: 6791 mean_circ: 0.937644
Ran for 116550 frames Cell Events: 7051 mean_circ: 0.937587
Ran for 118050 frames Cell Events: 7164 mean_circ: 0.937584
Ran for 123550 frames Cell Events: 7502 mean_circ: 0.937393
Ran for 124050 frames Cell Events: 7535 mean_circ: 0.937378
Ran for 124550 frames Cell Events: 7566 mean_circ: 0.937416
Ran for 126550 frames Cell Events: 7684 mean_circ: 0.937279
Ran for 127050 frames Cell Events: 7705 mean_circ: 0.937265
Ran for 128050 frames Cell Events: 7769 mean_circ: 0.937283
Ran for 128550 frames Cell Events: 7800 mean_circ: 0.937277
Ran for 129050 frames Cell Events: 7825 mean_circ: 0.937234
Ran for 129550 frames Cell Events: 7853 mean_circ: 0.937251
Ran for 132050 frames Cell Events: 8005 mean_circ: 0.937273
Ran for 132550 frames Cell Events: 8031 mean_circ: 0.937293
Ran for 134050 frames Cell Events: 8140 mean_circ: 0.93725
Ran for 135050 frames Cell Events: 8213 mean_circ: 0.93726



End this measurment..



Stopping time: 2.21054e+008
****************************************
Measurement finished

Mean Circularitiy: 0.937235
Mean Area: 605.685
Mean FL-1 intensity: 2892.03
Mean FL-2 intensity: 3823.82
Mean FL-3 intensity: 0

frames processed: 133347
Total Time: 67718.3ms
Number of Runs (new bg img) 0
Frames collected: 135227
Skipped frames: 40018
Rescued Frames: 3270778
Samples DAQ: 33807137
Samples written: 67613348
Samples per Channel: 33806674
FPS: 1996.91

Samples per second: 499225
Expected samples/s: 500000

Events: 8221
Events per second: 121.4
jumped back (PMT): 18
#Frames read, frequency
# 0 frames :218776
# 1 frames :39914
# 2 frames :2717
# 3 frames :965
# 4 frames :593
# 5 frames :305
# 6 frames :1301
# 7 frames :1050
# 8 frames :759
# 9 frames :571
# 10 frames :741
# 11 frames :373
# 12 frames :300
# 13 frames :211
# 14 frames :215
# 15 frames :212
# 16 frames :183
# 17 frames :140
# 18 frames :101
# 19 frames :111
# 20 frames :84
# 21 frames :82
# 22 frames :69
# 23 frames :71
# 24 frames :55
# 25 frames :44
# 26 frames :36
# 27 frames :35
# 28 frames :35
# 29 frames :29
# 30 frames :30
# 31 frames :22
# 32 frames :22
# 33 frames :18
# 34 frames :20
# 35 frames :17
# 36 frames :16
# 37 frames :8
# 38 frames :14
# 39 frames :11
# 40 frames :11
# 41 frames :7
# 42 frames :6
# 43 frames :12
# 44 frames :5
# 45 frames :4
# 46 frames :6
# 47 frames :5
# 48 frames :3
# 49 frames :4
# 50 frames :3
# 51 frames :4
# 52 frames :2
# 53 frames :3
# 54 frames :1
# 57 frames :1
# 58 frames :4
# 59 frames :3
# 60 frames :2
# 62 frames :1
# 63 frames :1
# 64 frames :1
# 66 frames :2
# 72 frames :2
# 73 frames :1
# 76 frames :1
# 78 frames :1
# 81 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.7223
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0