Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_18.20.23 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "aa91ce1b30d4aaeda46f17d9f9253690",
"name": "M1_20170616_1655_dsRNA_LZ_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "16:20:44"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8205 (max 8206) in '8205'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.8623
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.22284e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1550 frames Cell Events: 98 mean_circ: 0.94897
Ran for 2550 frames Cell Events: 160 mean_circ: 0.946341
Ran for 3050 frames Cell Events: 184 mean_circ: 0.948162
Ran for 3550 frames Cell Events: 201 mean_circ: 0.948539
Ran for 4550 frames Cell Events: 245 mean_circ: 0.947774
Ran for 5550 frames Cell Events: 299 mean_circ: 0.948569
Ran for 6050 frames Cell Events: 319 mean_circ: 0.948892
Ran for 7050 frames Cell Events: 359 mean_circ: 0.948398
Ran for 7550 frames Cell Events: 388 mean_circ: 0.949166
Ran for 8050 frames Cell Events: 410 mean_circ: 0.949599
Ran for 9050 frames Cell Events: 446 mean_circ: 0.948883
Ran for 9550 frames Cell Events: 472 mean_circ: 0.948945
Ran for 10550 frames Cell Events: 502 mean_circ: 0.948894
Ran for 13550 frames Cell Events: 646 mean_circ: 0.948281
Ran for 14050 frames Cell Events: 659 mean_circ: 0.947783
Ran for 17050 frames Cell Events: 792 mean_circ: 0.947414
Ran for 18050 frames Cell Events: 837 mean_circ: 0.947651
Ran for 19050 frames Cell Events: 865 mean_circ: 0.94791
Ran for 21050 frames Cell Events: 944 mean_circ: 0.947844
Ran for 22050 frames Cell Events: 978 mean_circ: 0.947704
Ran for 22550 frames Cell Events: 1000 mean_circ: 0.947915
Ran for 23050 frames Cell Events: 1023 mean_circ: 0.947903
Ran for 25550 frames Cell Events: 1125 mean_circ: 0.948182
Ran for 26550 frames Cell Events: 1178 mean_circ: 0.948294
Ran for 28050 frames Cell Events: 1235 mean_circ: 0.948211
Ran for 28550 frames Cell Events: 1247 mean_circ: 0.948416
Ran for 30050 frames Cell Events: 1304 mean_circ: 0.948248
Ran for 30550 frames Cell Events: 1316 mean_circ: 0.948224
Ran for 31550 frames Cell Events: 1339 mean_circ: 0.948194
Ran for 32550 frames Cell Events: 1381 mean_circ: 0.947957
Ran for 33050 frames Cell Events: 1398 mean_circ: 0.947782
Ran for 33550 frames Cell Events: 1417 mean_circ: 0.947749
Ran for 34050 frames Cell Events: 1434 mean_circ: 0.947856
Ran for 35050 frames Cell Events: 1478 mean_circ: 0.947725
Ran for 35550 frames Cell Events: 1491 mean_circ: 0.947767
Ran for 37050 frames Cell Events: 1560 mean_circ: 0.947868
Ran for 39550 frames Cell Events: 1697 mean_circ: 0.947941
Ran for 41050 frames Cell Events: 1776 mean_circ: 0.948155
Ran for 42050 frames Cell Events: 1816 mean_circ: 0.947904
Ran for 42550 frames Cell Events: 1839 mean_circ: 0.94788
Ran for 43050 frames Cell Events: 1856 mean_circ: 0.947982
Ran for 44050 frames Cell Events: 1904 mean_circ: 0.94802
Ran for 44550 frames Cell Events: 1922 mean_circ: 0.947895
Ran for 46050 frames Cell Events: 1995 mean_circ: 0.948071
Ran for 47050 frames Cell Events: 2033 mean_circ: 0.947917
Ran for 48050 frames Cell Events: 2079 mean_circ: 0.947695
Ran for 49050 frames Cell Events: 2121 mean_circ: 0.947865
Ran for 50550 frames Cell Events: 2167 mean_circ: 0.947905
Ran for 51550 frames Cell Events: 2209 mean_circ: 0.947989
Ran for 52550 frames Cell Events: 2262 mean_circ: 0.948135
Ran for 53550 frames Cell Events: 2298 mean_circ: 0.948065
Ran for 56050 frames Cell Events: 2392 mean_circ: 0.948045
Ran for 56550 frames Cell Events: 2419 mean_circ: 0.947827
Ran for 57050 frames Cell Events: 2449 mean_circ: 0.947876
Ran for 58550 frames Cell Events: 2518 mean_circ: 0.947896
Ran for 59550 frames Cell Events: 2551 mean_circ: 0.947994
Ran for 60550 frames Cell Events: 2590 mean_circ: 0.947943
Ran for 61050 frames Cell Events: 2611 mean_circ: 0.94793
Ran for 62050 frames Cell Events: 2646 mean_circ: 0.947883
Ran for 62550 frames Cell Events: 2667 mean_circ: 0.947964
Ran for 63050 frames Cell Events: 2685 mean_circ: 0.948
Ran for 63550 frames Cell Events: 2695 mean_circ: 0.948009
Ran for 65550 frames Cell Events: 2808 mean_circ: 0.947909
Ran for 66050 frames Cell Events: 2826 mean_circ: 0.947875
Ran for 66550 frames Cell Events: 2844 mean_circ: 0.947671
Ran for 67050 frames Cell Events: 2867 mean_circ: 0.947686
Ran for 67550 frames Cell Events: 2891 mean_circ: 0.947764
Ran for 68550 frames Cell Events: 2938 mean_circ: 0.947946
Ran for 69050 frames Cell Events: 2959 mean_circ: 0.947991
Ran for 70050 frames Cell Events: 2995 mean_circ: 0.948077
Ran for 72050 frames Cell Events: 3098 mean_circ: 0.948182
Ran for 72550 frames Cell Events: 3116 mean_circ: 0.948208
Ran for 73050 frames Cell Events: 3134 mean_circ: 0.948224
Ran for 73550 frames Cell Events: 3157 mean_circ: 0.948239
Ran for 74050 frames Cell Events: 3184 mean_circ: 0.948278
Ran for 75050 frames Cell Events: 3239 mean_circ: 0.948221
Ran for 76050 frames Cell Events: 3285 mean_circ: 0.94813
Ran for 76550 frames Cell Events: 3306 mean_circ: 0.94813
Ran for 77050 frames Cell Events: 3324 mean_circ: 0.948057
Ran for 77550 frames Cell Events: 3345 mean_circ: 0.948048
Ran for 78050 frames Cell Events: 3361 mean_circ: 0.948079
Ran for 78550 frames Cell Events: 3386 mean_circ: 0.948174
Ran for 79550 frames Cell Events: 3442 mean_circ: 0.948144
Ran for 80550 frames Cell Events: 3497 mean_circ: 0.948061
Ran for 81050 frames Cell Events: 3526 mean_circ: 0.947955
Ran for 81550 frames Cell Events: 3536 mean_circ: 0.947943
Ran for 84550 frames Cell Events: 3686 mean_circ: 0.948104
Ran for 85050 frames Cell Events: 3701 mean_circ: 0.948067
Ran for 85550 frames Cell Events: 3713 mean_circ: 0.94808
Ran for 86550 frames Cell Events: 3774 mean_circ: 0.948147
Ran for 87050 frames Cell Events: 3800 mean_circ: 0.948117
Ran for 89050 frames Cell Events: 3890 mean_circ: 0.947984
Ran for 89550 frames Cell Events: 3911 mean_circ: 0.947984
Ran for 90050 frames Cell Events: 3925 mean_circ: 0.948008
Ran for 90550 frames Cell Events: 3944 mean_circ: 0.94804
Ran for 92050 frames Cell Events: 4007 mean_circ: 0.948031
Ran for 92550 frames Cell Events: 4029 mean_circ: 0.948099
Ran for 93050 frames Cell Events: 4058 mean_circ: 0.948156
Ran for 93550 frames Cell Events: 4084 mean_circ: 0.948174
Ran for 94050 frames Cell Events: 4121 mean_circ: 0.948218
Ran for 94550 frames Cell Events: 4139 mean_circ: 0.948289
Ran for 95550 frames Cell Events: 4180 mean_circ: 0.948398
Ran for 96550 frames Cell Events: 4230 mean_circ: 0.948405
Ran for 97050 frames Cell Events: 4249 mean_circ: 0.948404
Ran for 97550 frames Cell Events: 4269 mean_circ: 0.948415
Ran for 98050 frames Cell Events: 4296 mean_circ: 0.948377
Ran for 100050 frames Cell Events: 4392 mean_circ: 0.948364
Ran for 100550 frames Cell Events: 4412 mean_circ: 0.948226
Ran for 101050 frames Cell Events: 4441 mean_circ: 0.948246
Ran for 102550 frames Cell Events: 4509 mean_circ: 0.948119
Ran for 103050 frames Cell Events: 4526 mean_circ: 0.948148
Ran for 105050 frames Cell Events: 4605 mean_circ: 0.948228
Ran for 106050 frames Cell Events: 4659 mean_circ: 0.948207
Ran for 107550 frames Cell Events: 4702 mean_circ: 0.948121
Ran for 108550 frames Cell Events: 4762 mean_circ: 0.94805
Ran for 109050 frames Cell Events: 4796 mean_circ: 0.948026
Ran for 109550 frames Cell Events: 4822 mean_circ: 0.948011
Ran for 110550 frames Cell Events: 4867 mean_circ: 0.947963
Ran for 111050 frames Cell Events: 4877 mean_circ: 0.947999
Ran for 111550 frames Cell Events: 4884 mean_circ: 0.947999
Ran for 112050 frames Cell Events: 4899 mean_circ: 0.947947
Ran for 112550 frames Cell Events: 4921 mean_circ: 0.947951
Ran for 113050 frames Cell Events: 4929 mean_circ: 0.94796
Ran for 114050 frames Cell Events: 4989 mean_circ: 0.947931
Ran for 114550 frames Cell Events: 5004 mean_circ: 0.947888
Ran for 115050 frames Cell Events: 5026 mean_circ: 0.947767
Ran for 115550 frames Cell Events: 5044 mean_circ: 0.947742
Ran for 116050 frames Cell Events: 5070 mean_circ: 0.947747
Ran for 116550 frames Cell Events: 5100 mean_circ: 0.947721
Ran for 118550 frames Cell Events: 5190 mean_circ: 0.947609
Ran for 120550 frames Cell Events: 5288 mean_circ: 0.947541
Ran for 121050 frames Cell Events: 5315 mean_circ: 0.947541
Ran for 123050 frames Cell Events: 5445 mean_circ: 0.947375
Ran for 123550 frames Cell Events: 5463 mean_circ: 0.947351
Ran for 124050 frames Cell Events: 5488 mean_circ: 0.947364
Ran for 124550 frames Cell Events: 5511 mean_circ: 0.947411
Ran for 125050 frames Cell Events: 5532 mean_circ: 0.947399
Ran for 125550 frames Cell Events: 5554 mean_circ: 0.947393
Ran for 127550 frames Cell Events: 5651 mean_circ: 0.947413
Ran for 129050 frames Cell Events: 5732 mean_circ: 0.947482
Ran for 130050 frames Cell Events: 5755 mean_circ: 0.94749
Ran for 130550 frames Cell Events: 5785 mean_circ: 0.947476
Ran for 131050 frames Cell Events: 5808 mean_circ: 0.947495
Ran for 131550 frames Cell Events: 5836 mean_circ: 0.947506
Ran for 132050 frames Cell Events: 5858 mean_circ: 0.947457
Ran for 132550 frames Cell Events: 5888 mean_circ: 0.947456
Ran for 133550 frames Cell Events: 5946 mean_circ: 0.947483
Ran for 135050 frames Cell Events: 6012 mean_circ: 0.947573
Ran for 137050 frames Cell Events: 6113 mean_circ: 0.947623
Ran for 137550 frames Cell Events: 6127 mean_circ: 0.947616
Ran for 138050 frames Cell Events: 6149 mean_circ: 0.947668
Ran for 138550 frames Cell Events: 6175 mean_circ: 0.947633
Ran for 140050 frames Cell Events: 6243 mean_circ: 0.947575
Ran for 140550 frames Cell Events: 6261 mean_circ: 0.947602
Ran for 141050 frames Cell Events: 6289 mean_circ: 0.947567
Ran for 141550 frames Cell Events: 6312 mean_circ: 0.947597
Ran for 142050 frames Cell Events: 6329 mean_circ: 0.947612
Ran for 142550 frames Cell Events: 6353 mean_circ: 0.94761
Ran for 143050 frames Cell Events: 6368 mean_circ: 0.947611
Ran for 143550 frames Cell Events: 6390 mean_circ: 0.947633
Ran for 144550 frames Cell Events: 6445 mean_circ: 0.947717
Ran for 145050 frames Cell Events: 6474 mean_circ: 0.947707
Ran for 145550 frames Cell Events: 6495 mean_circ: 0.947696
Ran for 146050 frames Cell Events: 6509 mean_circ: 0.947711
Ran for 146550 frames Cell Events: 6533 mean_circ: 0.947713
Ran for 147550 frames Cell Events: 6576 mean_circ: 0.947732
Ran for 148050 frames Cell Events: 6600 mean_circ: 0.947747
Ran for 150050 frames Cell Events: 6679 mean_circ: 0.947744
Ran for 150550 frames Cell Events: 6700 mean_circ: 0.947695
Ran for 151050 frames Cell Events: 6719 mean_circ: 0.947701
Ran for 151550 frames Cell Events: 6741 mean_circ: 0.947701
Ran for 152550 frames Cell Events: 6780 mean_circ: 0.947712
Ran for 153050 frames Cell Events: 6800 mean_circ: 0.947697
Ran for 154050 frames Cell Events: 6835 mean_circ: 0.947664
Ran for 156550 frames Cell Events: 6939 mean_circ: 0.947686
Ran for 157550 frames Cell Events: 6975 mean_circ: 0.94764
Ran for 158050 frames Cell Events: 6998 mean_circ: 0.947644
Ran for 159550 frames Cell Events: 7097 mean_circ: 0.947551
Ran for 160050 frames Cell Events: 7114 mean_circ: 0.947594
Ran for 160550 frames Cell Events: 7129 mean_circ: 0.94761
Ran for 161550 frames Cell Events: 7175 mean_circ: 0.947594
Ran for 162550 frames Cell Events: 7228 mean_circ: 0.947592
Ran for 163550 frames Cell Events: 7289 mean_circ: 0.947608
Ran for 165050 frames Cell Events: 7355 mean_circ: 0.947538
Ran for 166050 frames Cell Events: 7403 mean_circ: 0.94754
Ran for 166550 frames Cell Events: 7426 mean_circ: 0.947499
Ran for 167050 frames Cell Events: 7457 mean_circ: 0.947491
Ran for 168050 frames Cell Events: 7502 mean_circ: 0.947511
Ran for 168550 frames Cell Events: 7525 mean_circ: 0.947548
Ran for 169550 frames Cell Events: 7564 mean_circ: 0.947549
Ran for 171050 frames Cell Events: 7645 mean_circ: 0.94743
Ran for 171550 frames Cell Events: 7680 mean_circ: 0.947394
Ran for 172050 frames Cell Events: 7703 mean_circ: 0.947399
Ran for 173050 frames Cell Events: 7752 mean_circ: 0.947384
Ran for 173550 frames Cell Events: 7769 mean_circ: 0.947444
Ran for 174050 frames Cell Events: 7797 mean_circ: 0.947472
Ran for 174550 frames Cell Events: 7826 mean_circ: 0.947435
Ran for 175550 frames Cell Events: 7866 mean_circ: 0.94742
Ran for 176550 frames Cell Events: 7916 mean_circ: 0.947437
Ran for 177550 frames Cell Events: 7971 mean_circ: 0.947431
Ran for 178050 frames Cell Events: 7993 mean_circ: 0.947396
Ran for 180550 frames Cell Events: 8108 mean_circ: 0.947441
Ran for 181050 frames Cell Events: 8131 mean_circ: 0.947428



End this measurment..



Stopping time: 2.22376e+008
****************************************
Measurement finished

Mean Circularitiy: 0.947494
Mean Area: 620.952
Mean FL-1 intensity: 3039.33
Mean FL-2 intensity: 3754.52
Mean FL-3 intensity: 0

frames processed: 180769
Total Time: 91388.1ms
Number of Runs (new bg img) 0
Frames collected: 182605
Skipped frames: 43241
Rescued Frames: 3551249
Samples DAQ: 45651297
Samples written: 91302340
Samples per Channel: 45651170
FPS: 1998.13

Samples per second: 499531
Expected samples/s: 500000

Events: 8206
Events per second: 89.7929
jumped back (PMT): 4054
#Frames read, frequency
# 0 frames :278799
# 1 frames :70998
# 2 frames :3707
# 3 frames :1448
# 4 frames :926
# 5 frames :464
# 6 frames :1296
# 7 frames :1349
# 8 frames :993
# 9 frames :634
# 10 frames :916
# 11 frames :522
# 12 frames :387
# 13 frames :284
# 14 frames :251
# 15 frames :204
# 16 frames :212
# 17 frames :170
# 18 frames :156
# 19 frames :105
# 20 frames :94
# 21 frames :89
# 22 frames :78
# 23 frames :66
# 24 frames :61
# 25 frames :46
# 26 frames :45
# 27 frames :40
# 28 frames :30
# 29 frames :22
# 30 frames :27
# 31 frames :16
# 32 frames :18
# 33 frames :16
# 34 frames :9
# 35 frames :18
# 36 frames :9
# 37 frames :16
# 38 frames :6
# 39 frames :9
# 40 frames :5
# 41 frames :4
# 42 frames :10
# 43 frames :5
# 44 frames :5
# 45 frames :4
# 46 frames :7
# 47 frames :3
# 48 frames :2
# 49 frames :1
# 50 frames :1
# 51 frames :4
# 52 frames :4
# 53 frames :2
# 54 frames :3
# 55 frames :2
# 56 frames :1
# 57 frames :1
# 58 frames :1
# 59 frames :1
# 60 frames :2
# 61 frames :2
# 63 frames :2
# 64 frames :1
# 70 frames :1
# 71 frames :1
# 75 frames :1
# 79 frames :1
# 89 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.8623
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0