Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_18.20.51 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "e65585fb129875dbb127c4655254c6ee",
"name": "M1_20170616_1659_dsRNA_LZ_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "16:21:12"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8533 (max 8534) in '8533'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.91
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.22508e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 49 mean_circ: 0.935445
Ran for 2550 frames Cell Events: 110 mean_circ: 0.931081
Ran for 3050 frames Cell Events: 127 mean_circ: 0.931651
Ran for 4050 frames Cell Events: 165 mean_circ: 0.930051
Ran for 4550 frames Cell Events: 185 mean_circ: 0.930725
Ran for 5050 frames Cell Events: 211 mean_circ: 0.931045
Ran for 7050 frames Cell Events: 294 mean_circ: 0.93308
Ran for 8050 frames Cell Events: 353 mean_circ: 0.935295
Ran for 8550 frames Cell Events: 374 mean_circ: 0.935616
Ran for 9050 frames Cell Events: 403 mean_circ: 0.935574
Ran for 9550 frames Cell Events: 434 mean_circ: 0.935776
Ran for 10050 frames Cell Events: 464 mean_circ: 0.935216
Ran for 10550 frames Cell Events: 476 mean_circ: 0.934668
Ran for 11050 frames Cell Events: 497 mean_circ: 0.935027
Ran for 12050 frames Cell Events: 536 mean_circ: 0.934922
Ran for 13050 frames Cell Events: 584 mean_circ: 0.935066
Ran for 15050 frames Cell Events: 676 mean_circ: 0.93464
Ran for 15550 frames Cell Events: 693 mean_circ: 0.934923
Ran for 17050 frames Cell Events: 759 mean_circ: 0.935074
Ran for 18550 frames Cell Events: 839 mean_circ: 0.93527
Ran for 19550 frames Cell Events: 874 mean_circ: 0.935352
Ran for 20050 frames Cell Events: 902 mean_circ: 0.935419
Ran for 21550 frames Cell Events: 1001 mean_circ: 0.93512
Ran for 22550 frames Cell Events: 1048 mean_circ: 0.935016
Ran for 25050 frames Cell Events: 1151 mean_circ: 0.93504
Ran for 25550 frames Cell Events: 1172 mean_circ: 0.934722
Ran for 26050 frames Cell Events: 1193 mean_circ: 0.934782
Ran for 27550 frames Cell Events: 1274 mean_circ: 0.935272
Ran for 28050 frames Cell Events: 1298 mean_circ: 0.935334
Ran for 29050 frames Cell Events: 1361 mean_circ: 0.935528
Ran for 31050 frames Cell Events: 1456 mean_circ: 0.935375
Ran for 31550 frames Cell Events: 1474 mean_circ: 0.93543
Ran for 33050 frames Cell Events: 1555 mean_circ: 0.935493
Ran for 33550 frames Cell Events: 1574 mean_circ: 0.935532
Ran for 34050 frames Cell Events: 1596 mean_circ: 0.935501
Ran for 35050 frames Cell Events: 1652 mean_circ: 0.935753
Ran for 36050 frames Cell Events: 1691 mean_circ: 0.93567
Ran for 36550 frames Cell Events: 1714 mean_circ: 0.935661
Ran for 37050 frames Cell Events: 1732 mean_circ: 0.935481
Ran for 37550 frames Cell Events: 1750 mean_circ: 0.93534
Ran for 38050 frames Cell Events: 1783 mean_circ: 0.935335
Ran for 39550 frames Cell Events: 1853 mean_circ: 0.935483
Ran for 40050 frames Cell Events: 1877 mean_circ: 0.935395
Ran for 41050 frames Cell Events: 1921 mean_circ: 0.935583
Ran for 42050 frames Cell Events: 1987 mean_circ: 0.935264
Ran for 44550 frames Cell Events: 2111 mean_circ: 0.935208
Ran for 46550 frames Cell Events: 2208 mean_circ: 0.935221
Ran for 47050 frames Cell Events: 2226 mean_circ: 0.935265
Ran for 47550 frames Cell Events: 2251 mean_circ: 0.93543
Ran for 49050 frames Cell Events: 2341 mean_circ: 0.935196
Ran for 50050 frames Cell Events: 2385 mean_circ: 0.935219
Ran for 51050 frames Cell Events: 2451 mean_circ: 0.935296
Ran for 52550 frames Cell Events: 2539 mean_circ: 0.935228
Ran for 53050 frames Cell Events: 2556 mean_circ: 0.935145
Ran for 53550 frames Cell Events: 2575 mean_circ: 0.935163
Ran for 54550 frames Cell Events: 2623 mean_circ: 0.935151
Ran for 55050 frames Cell Events: 2639 mean_circ: 0.935086
Ran for 56050 frames Cell Events: 2693 mean_circ: 0.935156
Ran for 57050 frames Cell Events: 2742 mean_circ: 0.935155
Ran for 58050 frames Cell Events: 2785 mean_circ: 0.935148
Ran for 59050 frames Cell Events: 2837 mean_circ: 0.935078
Ran for 60550 frames Cell Events: 2905 mean_circ: 0.935042
Ran for 61050 frames Cell Events: 2934 mean_circ: 0.934969
Ran for 61550 frames Cell Events: 2959 mean_circ: 0.93499
Ran for 62550 frames Cell Events: 2999 mean_circ: 0.935059
Ran for 64050 frames Cell Events: 3061 mean_circ: 0.935261
Ran for 65050 frames Cell Events: 3118 mean_circ: 0.935265
Ran for 66550 frames Cell Events: 3211 mean_circ: 0.935275
Ran for 67550 frames Cell Events: 3256 mean_circ: 0.935174
Ran for 68550 frames Cell Events: 3310 mean_circ: 0.935236
Ran for 70050 frames Cell Events: 3378 mean_circ: 0.934938
Ran for 71550 frames Cell Events: 3458 mean_circ: 0.935217
Ran for 73050 frames Cell Events: 3546 mean_circ: 0.935007
Ran for 73550 frames Cell Events: 3563 mean_circ: 0.935018
Ran for 74050 frames Cell Events: 3593 mean_circ: 0.935074
Ran for 74550 frames Cell Events: 3615 mean_circ: 0.934974
Ran for 75050 frames Cell Events: 3635 mean_circ: 0.935014
Ran for 75550 frames Cell Events: 3663 mean_circ: 0.935042
Ran for 77550 frames Cell Events: 3765 mean_circ: 0.935226
Ran for 78550 frames Cell Events: 3808 mean_circ: 0.93531
Ran for 79050 frames Cell Events: 3823 mean_circ: 0.935352
Ran for 79550 frames Cell Events: 3849 mean_circ: 0.935399
Ran for 81050 frames Cell Events: 3904 mean_circ: 0.935408
Ran for 83550 frames Cell Events: 4033 mean_circ: 0.935454
Ran for 84050 frames Cell Events: 4051 mean_circ: 0.935516
Ran for 85550 frames Cell Events: 4137 mean_circ: 0.935634
Ran for 86050 frames Cell Events: 4156 mean_circ: 0.935636
Ran for 88050 frames Cell Events: 4297 mean_circ: 0.935441
Ran for 88550 frames Cell Events: 4328 mean_circ: 0.935542
Ran for 89050 frames Cell Events: 4351 mean_circ: 0.935542
Ran for 89550 frames Cell Events: 4375 mean_circ: 0.935628
Ran for 92050 frames Cell Events: 4516 mean_circ: 0.935571
Ran for 92550 frames Cell Events: 4540 mean_circ: 0.935543
Ran for 93050 frames Cell Events: 4579 mean_circ: 0.935445
Ran for 94550 frames Cell Events: 4653 mean_circ: 0.935383
Ran for 95050 frames Cell Events: 4686 mean_circ: 0.935432
Ran for 95550 frames Cell Events: 4713 mean_circ: 0.935443
Ran for 96550 frames Cell Events: 4763 mean_circ: 0.935511
Ran for 97050 frames Cell Events: 4783 mean_circ: 0.935514
Ran for 99050 frames Cell Events: 4876 mean_circ: 0.935523
Ran for 100050 frames Cell Events: 4932 mean_circ: 0.935602
Ran for 101050 frames Cell Events: 4977 mean_circ: 0.935562
Ran for 103050 frames Cell Events: 5061 mean_circ: 0.93543
Ran for 103550 frames Cell Events: 5080 mean_circ: 0.935471
Ran for 104550 frames Cell Events: 5147 mean_circ: 0.935414
Ran for 105550 frames Cell Events: 5217 mean_circ: 0.935459
Ran for 108050 frames Cell Events: 5358 mean_circ: 0.935392
Ran for 109050 frames Cell Events: 5414 mean_circ: 0.935388
Ran for 110050 frames Cell Events: 5464 mean_circ: 0.935307
Ran for 110550 frames Cell Events: 5493 mean_circ: 0.935233
Ran for 111050 frames Cell Events: 5521 mean_circ: 0.935257
Ran for 112550 frames Cell Events: 5616 mean_circ: 0.935188
Ran for 113050 frames Cell Events: 5645 mean_circ: 0.935188
Ran for 113550 frames Cell Events: 5674 mean_circ: 0.93516
Ran for 114550 frames Cell Events: 5728 mean_circ: 0.935112
Ran for 115050 frames Cell Events: 5751 mean_circ: 0.935094
Ran for 115550 frames Cell Events: 5778 mean_circ: 0.935023
Ran for 117050 frames Cell Events: 5844 mean_circ: 0.93508
Ran for 117550 frames Cell Events: 5856 mean_circ: 0.935079
Ran for 118050 frames Cell Events: 5882 mean_circ: 0.935054
Ran for 118550 frames Cell Events: 5902 mean_circ: 0.935014
Ran for 119550 frames Cell Events: 5953 mean_circ: 0.935065
Ran for 121550 frames Cell Events: 6057 mean_circ: 0.935066
Ran for 122050 frames Cell Events: 6076 mean_circ: 0.935069
Ran for 123050 frames Cell Events: 6121 mean_circ: 0.935085
Ran for 123550 frames Cell Events: 6145 mean_circ: 0.9351
Ran for 124550 frames Cell Events: 6209 mean_circ: 0.935153
Ran for 127550 frames Cell Events: 6397 mean_circ: 0.93504
Ran for 130550 frames Cell Events: 6544 mean_circ: 0.935188
Ran for 132050 frames Cell Events: 6626 mean_circ: 0.935251
Ran for 132550 frames Cell Events: 6652 mean_circ: 0.935249
Ran for 133550 frames Cell Events: 6695 mean_circ: 0.935141
Ran for 134550 frames Cell Events: 6728 mean_circ: 0.935073
Ran for 135050 frames Cell Events: 6757 mean_circ: 0.935073
Ran for 136050 frames Cell Events: 6814 mean_circ: 0.935082
Ran for 136550 frames Cell Events: 6833 mean_circ: 0.935053
Ran for 137050 frames Cell Events: 6852 mean_circ: 0.935047
Ran for 138050 frames Cell Events: 6921 mean_circ: 0.935015
Ran for 139550 frames Cell Events: 7006 mean_circ: 0.935002
Ran for 140550 frames Cell Events: 7079 mean_circ: 0.935002
Ran for 142050 frames Cell Events: 7151 mean_circ: 0.93506
Ran for 142550 frames Cell Events: 7177 mean_circ: 0.935082
Ran for 143550 frames Cell Events: 7251 mean_circ: 0.935061
Ran for 145550 frames Cell Events: 7370 mean_circ: 0.935071
Ran for 147050 frames Cell Events: 7436 mean_circ: 0.93505
Ran for 149550 frames Cell Events: 7593 mean_circ: 0.935043
Ran for 151550 frames Cell Events: 7711 mean_circ: 0.935061
Ran for 152050 frames Cell Events: 7735 mean_circ: 0.93512
Ran for 152550 frames Cell Events: 7765 mean_circ: 0.935042
Ran for 153550 frames Cell Events: 7818 mean_circ: 0.935066
Ran for 154550 frames Cell Events: 7870 mean_circ: 0.935078
Ran for 155050 frames Cell Events: 7885 mean_circ: 0.935121
Ran for 156050 frames Cell Events: 7928 mean_circ: 0.935132
Ran for 156550 frames Cell Events: 7955 mean_circ: 0.935114
Ran for 157050 frames Cell Events: 7990 mean_circ: 0.935131
Ran for 159050 frames Cell Events: 8109 mean_circ: 0.935035
Ran for 162050 frames Cell Events: 8259 mean_circ: 0.93499
Ran for 163050 frames Cell Events: 8304 mean_circ: 0.934972
Ran for 164550 frames Cell Events: 8373 mean_circ: 0.934989
Ran for 165050 frames Cell Events: 8401 mean_circ: 0.934955
Ran for 166550 frames Cell Events: 8483 mean_circ: 0.93499
Ran for 167050 frames Cell Events: 8501 mean_circ: 0.935003
Ran for 167550 frames Cell Events: 8530 mean_circ: 0.934996



End this measurment..



Stopping time: 2.22592e+008
****************************************
Measurement finished

Mean Circularitiy: 0.935013
Mean Area: 622.655
Mean FL-1 intensity: 2939.24
Mean FL-2 intensity: 4087.1
Mean FL-3 intensity: 0

frames processed: 166106
Total Time: 83890.3ms
Number of Runs (new bg img) 0
Frames collected: 167667
Skipped frames: 44802
Rescued Frames: 3643947
Samples DAQ: 41916878
Samples written: 83833674
Samples per Channel: 41916837
FPS: 1998.65

Samples per second: 499662
Expected samples/s: 500000

Events: 8534
Events per second: 101.728
jumped back (PMT): 19
#Frames read, frequency
# 0 frames :261881
# 1 frames :59099
# 2 frames :3656
# 3 frames :1311
# 4 frames :801
# 5 frames :432
# 6 frames :1190
# 7 frames :1533
# 8 frames :919
# 9 frames :656
# 10 frames :909
# 11 frames :472
# 12 frames :364
# 13 frames :272
# 14 frames :205
# 15 frames :230
# 16 frames :212
# 17 frames :164
# 18 frames :150
# 19 frames :96
# 20 frames :86
# 21 frames :76
# 22 frames :64
# 23 frames :55
# 24 frames :55
# 25 frames :65
# 26 frames :43
# 27 frames :33
# 28 frames :25
# 29 frames :25
# 30 frames :20
# 31 frames :26
# 32 frames :16
# 33 frames :16
# 34 frames :16
# 35 frames :10
# 36 frames :15
# 37 frames :13
# 38 frames :6
# 39 frames :12
# 40 frames :6
# 41 frames :11
# 42 frames :7
# 43 frames :6
# 44 frames :9
# 45 frames :2
# 46 frames :3
# 47 frames :5
# 48 frames :5
# 49 frames :6
# 51 frames :5
# 52 frames :6
# 54 frames :2
# 55 frames :2
# 56 frames :1
# 57 frames :2
# 58 frames :1
# 59 frames :2
# 62 frames :1
# 63 frames :1
# 66 frames :1
# 72 frames :1
# 74 frames :1
# 93 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.91
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0