Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_18.27.25 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "31358acc8f232ba1bb2723bd71bf80c5",
"name": "M1_20170616_1833_dsRNA_Z4_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "16:27:45"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8114 (max 8115) in '8114'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.126
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.28133e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 1 mean_circ: 0.942612
Ran for 1550 frames Cell Events: 97 mean_circ: 0.914436
Ran for 2550 frames Cell Events: 149 mean_circ: 0.92016
Ran for 5550 frames Cell Events: 268 mean_circ: 0.929044
Ran for 7050 frames Cell Events: 343 mean_circ: 0.928812
Ran for 7550 frames Cell Events: 370 mean_circ: 0.929523
Ran for 8550 frames Cell Events: 414 mean_circ: 0.929539
Ran for 9050 frames Cell Events: 432 mean_circ: 0.929897
Ran for 10050 frames Cell Events: 442 mean_circ: 0.930514
Ran for 10550 frames Cell Events: 460 mean_circ: 0.931029
Ran for 11550 frames Cell Events: 498 mean_circ: 0.931504
Ran for 12050 frames Cell Events: 514 mean_circ: 0.932088
Ran for 12550 frames Cell Events: 539 mean_circ: 0.932142
Ran for 13550 frames Cell Events: 598 mean_circ: 0.932352
Ran for 14550 frames Cell Events: 666 mean_circ: 0.932865
Ran for 16050 frames Cell Events: 758 mean_circ: 0.932249
Ran for 17550 frames Cell Events: 845 mean_circ: 0.931692
Ran for 18050 frames Cell Events: 863 mean_circ: 0.931652
Ran for 18550 frames Cell Events: 902 mean_circ: 0.931184
Ran for 20050 frames Cell Events: 996 mean_circ: 0.931319
Ran for 21550 frames Cell Events: 1089 mean_circ: 0.93002
Ran for 22550 frames Cell Events: 1136 mean_circ: 0.930051
Ran for 23050 frames Cell Events: 1159 mean_circ: 0.929864
Ran for 24550 frames Cell Events: 1239 mean_circ: 0.930149
Ran for 25050 frames Cell Events: 1277 mean_circ: 0.930323
Ran for 27550 frames Cell Events: 1371 mean_circ: 0.92989
Ran for 28050 frames Cell Events: 1397 mean_circ: 0.930087
Ran for 28550 frames Cell Events: 1431 mean_circ: 0.930283
Ran for 29050 frames Cell Events: 1453 mean_circ: 0.930419
Ran for 30050 frames Cell Events: 1519 mean_circ: 0.930676
Ran for 30550 frames Cell Events: 1554 mean_circ: 0.930654
Ran for 31050 frames Cell Events: 1583 mean_circ: 0.930838
Ran for 36050 frames Cell Events: 1963 mean_circ: 0.930346
Ran for 36550 frames Cell Events: 1989 mean_circ: 0.930538
Ran for 37550 frames Cell Events: 2083 mean_circ: 0.93036
Ran for 38550 frames Cell Events: 2158 mean_circ: 0.930532
Ran for 39550 frames Cell Events: 2218 mean_circ: 0.930591
Ran for 40050 frames Cell Events: 2240 mean_circ: 0.930403
Ran for 40550 frames Cell Events: 2262 mean_circ: 0.930491
Ran for 42550 frames Cell Events: 2388 mean_circ: 0.930688
Ran for 43550 frames Cell Events: 2474 mean_circ: 0.93031
Ran for 45050 frames Cell Events: 2591 mean_circ: 0.930233
Ran for 46050 frames Cell Events: 2658 mean_circ: 0.929962
Ran for 47050 frames Cell Events: 2730 mean_circ: 0.929971
Ran for 47550 frames Cell Events: 2753 mean_circ: 0.929872
Ran for 48050 frames Cell Events: 2781 mean_circ: 0.930013
Ran for 49050 frames Cell Events: 2858 mean_circ: 0.930238
Ran for 49550 frames Cell Events: 2886 mean_circ: 0.930143
Ran for 51550 frames Cell Events: 3011 mean_circ: 0.930047
Ran for 52550 frames Cell Events: 3053 mean_circ: 0.930156
Ran for 53550 frames Cell Events: 3110 mean_circ: 0.93025
Ran for 54050 frames Cell Events: 3141 mean_circ: 0.930253
Ran for 54550 frames Cell Events: 3174 mean_circ: 0.930433
Ran for 56050 frames Cell Events: 3307 mean_circ: 0.930433
Ran for 56550 frames Cell Events: 3347 mean_circ: 0.930504
Ran for 57550 frames Cell Events: 3446 mean_circ: 0.930211
Ran for 58050 frames Cell Events: 3478 mean_circ: 0.930152
Ran for 59050 frames Cell Events: 3551 mean_circ: 0.929923
Ran for 59550 frames Cell Events: 3589 mean_circ: 0.929929
Ran for 61050 frames Cell Events: 3699 mean_circ: 0.929594
Ran for 63050 frames Cell Events: 3858 mean_circ: 0.929583
Ran for 63550 frames Cell Events: 3886 mean_circ: 0.929718
Ran for 65050 frames Cell Events: 3991 mean_circ: 0.929881
Ran for 66050 frames Cell Events: 4045 mean_circ: 0.929817
Ran for 66550 frames Cell Events: 4063 mean_circ: 0.929885
Ran for 67050 frames Cell Events: 4089 mean_circ: 0.929818
Ran for 68050 frames Cell Events: 4146 mean_circ: 0.929727
Ran for 69050 frames Cell Events: 4215 mean_circ: 0.929741
Ran for 69550 frames Cell Events: 4246 mean_circ: 0.929824
Ran for 70050 frames Cell Events: 4275 mean_circ: 0.929743
Ran for 71050 frames Cell Events: 4308 mean_circ: 0.929705
Ran for 75050 frames Cell Events: 4511 mean_circ: 0.929692
Ran for 75550 frames Cell Events: 4523 mean_circ: 0.929657
Ran for 76050 frames Cell Events: 4543 mean_circ: 0.929647
Ran for 77050 frames Cell Events: 4584 mean_circ: 0.929638
Ran for 77550 frames Cell Events: 4613 mean_circ: 0.929578
Ran for 78050 frames Cell Events: 4642 mean_circ: 0.929604
Ran for 78550 frames Cell Events: 4668 mean_circ: 0.929415
Ran for 79050 frames Cell Events: 4700 mean_circ: 0.929355
Ran for 80050 frames Cell Events: 4754 mean_circ: 0.929372
Ran for 81050 frames Cell Events: 4814 mean_circ: 0.92959
Ran for 83050 frames Cell Events: 4954 mean_circ: 0.929572
Ran for 83550 frames Cell Events: 4987 mean_circ: 0.929574
Ran for 85050 frames Cell Events: 5081 mean_circ: 0.929711
Ran for 85550 frames Cell Events: 5104 mean_circ: 0.929717
Ran for 87050 frames Cell Events: 5192 mean_circ: 0.929589
Ran for 90050 frames Cell Events: 5398 mean_circ: 0.929379
Ran for 90550 frames Cell Events: 5436 mean_circ: 0.92941
Ran for 91550 frames Cell Events: 5517 mean_circ: 0.929461
Ran for 92050 frames Cell Events: 5544 mean_circ: 0.929492
Ran for 93050 frames Cell Events: 5595 mean_circ: 0.929526
Ran for 94050 frames Cell Events: 5646 mean_circ: 0.929473
Ran for 94550 frames Cell Events: 5672 mean_circ: 0.92948
Ran for 95050 frames Cell Events: 5706 mean_circ: 0.929461
Ran for 96050 frames Cell Events: 5754 mean_circ: 0.929522
Ran for 96550 frames Cell Events: 5774 mean_circ: 0.929492
Ran for 97050 frames Cell Events: 5812 mean_circ: 0.929504
Ran for 97550 frames Cell Events: 5840 mean_circ: 0.929501
Ran for 98550 frames Cell Events: 5886 mean_circ: 0.929525
Ran for 99550 frames Cell Events: 5943 mean_circ: 0.929548
Ran for 100550 frames Cell Events: 5969 mean_circ: 0.929563
Ran for 101550 frames Cell Events: 6021 mean_circ: 0.929523
Ran for 102050 frames Cell Events: 6055 mean_circ: 0.929523
Ran for 102550 frames Cell Events: 6076 mean_circ: 0.929543
Ran for 103550 frames Cell Events: 6141 mean_circ: 0.929545
Ran for 106550 frames Cell Events: 6247 mean_circ: 0.929303
Ran for 108550 frames Cell Events: 6394 mean_circ: 0.929237
Ran for 109050 frames Cell Events: 6432 mean_circ: 0.929213
Ran for 113550 frames Cell Events: 6810 mean_circ: 0.928521
Ran for 117550 frames Cell Events: 7078 mean_circ: 0.928685
Ran for 118550 frames Cell Events: 7146 mean_circ: 0.928696
Ran for 119550 frames Cell Events: 7212 mean_circ: 0.928747
Ran for 120050 frames Cell Events: 7249 mean_circ: 0.928776
Ran for 121050 frames Cell Events: 7311 mean_circ: 0.928782
Ran for 121550 frames Cell Events: 7344 mean_circ: 0.92876
Ran for 127550 frames Cell Events: 7727 mean_circ: 0.928755
Ran for 128550 frames Cell Events: 7769 mean_circ: 0.928826
Ran for 129550 frames Cell Events: 7829 mean_circ: 0.928766
Ran for 130550 frames Cell Events: 7870 mean_circ: 0.928753
Ran for 131050 frames Cell Events: 7894 mean_circ: 0.928758
Ran for 131550 frames Cell Events: 7919 mean_circ: 0.928802
Ran for 132050 frames Cell Events: 7953 mean_circ: 0.928805
Ran for 133050 frames Cell Events: 8014 mean_circ: 0.928733
Ran for 133550 frames Cell Events: 8037 mean_circ: 0.928745
Ran for 134050 frames Cell Events: 8056 mean_circ: 0.928715
Ran for 135050 frames Cell Events: 8115 mean_circ: 0.928731



End this measurment..



Stopping time: 2.282e+008
****************************************
Measurement finished

Mean Circularitiy: 0.928746
Mean Area: 624.868
Mean FL-1 intensity: 2906.81
Mean FL-2 intensity: 3525.53
Mean FL-3 intensity: 0

frames processed: 128606
Total Time: 67621.2ms
Number of Runs (new bg img) 0
Frames collected: 135076
Skipped frames: 63585
Rescued Frames: 5102303
Samples DAQ: 33769124
Samples written: 67538082
Samples per Channel: 33769041
FPS: 1997.54

Samples per second: 499385
Expected samples/s: 500000

Events: 8115
Events per second: 120.007
jumped back (PMT): 40
#Frames read, frequency
# 0 frames :216430
# 1 frames :37232
# 2 frames :2331
# 3 frames :970
# 4 frames :588
# 5 frames :311
# 6 frames :1387
# 7 frames :989
# 8 frames :808
# 9 frames :524
# 10 frames :416
# 11 frames :320
# 12 frames :263
# 13 frames :211
# 14 frames :213
# 15 frames :197
# 16 frames :187
# 17 frames :116
# 18 frames :116
# 19 frames :91
# 20 frames :87
# 21 frames :90
# 22 frames :79
# 23 frames :70
# 24 frames :57
# 25 frames :52
# 26 frames :41
# 27 frames :40
# 28 frames :49
# 29 frames :29
# 30 frames :34
# 31 frames :34
# 32 frames :20
# 33 frames :22
# 34 frames :18
# 35 frames :14
# 36 frames :12
# 37 frames :19
# 38 frames :23
# 39 frames :16
# 40 frames :8
# 41 frames :10
# 42 frames :10
# 43 frames :15
# 44 frames :6
# 45 frames :9
# 46 frames :9
# 47 frames :8
# 48 frames :2
# 49 frames :7
# 50 frames :6
# 51 frames :8
# 52 frames :2
# 53 frames :3
# 54 frames :2
# 55 frames :2
# 56 frames :4
# 57 frames :2
# 59 frames :1
# 60 frames :3
# 62 frames :1
# 63 frames :4
# 64 frames :3
# 66 frames :2
# 67 frames :1
# 69 frames :2
# 70 frames :2
# 73 frames :1
# 74 frames :1
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# 92 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.126
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0