Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_18.32.06 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "79fc02537c891c3425bc7b1a5eb86956",
"name": "M1_20170616_1948_dsRNA_Q8_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "16:32:27"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8645 (max 8646) in '8645'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.6131
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.32686e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 30 mean_circ: 0.953505
Ran for 3050 frames Cell Events: 156 mean_circ: 0.94897
Ran for 3550 frames Cell Events: 180 mean_circ: 0.949586
Ran for 5050 frames Cell Events: 262 mean_circ: 0.949379
Ran for 8550 frames Cell Events: 462 mean_circ: 0.950407
Ran for 9050 frames Cell Events: 485 mean_circ: 0.949829
Ran for 9550 frames Cell Events: 518 mean_circ: 0.950145
Ran for 11050 frames Cell Events: 621 mean_circ: 0.949575
Ran for 11550 frames Cell Events: 648 mean_circ: 0.949575
Ran for 12050 frames Cell Events: 670 mean_circ: 0.950154
Ran for 13550 frames Cell Events: 745 mean_circ: 0.949619
Ran for 17050 frames Cell Events: 937 mean_circ: 0.950167
Ran for 18050 frames Cell Events: 986 mean_circ: 0.950133
Ran for 20550 frames Cell Events: 1119 mean_circ: 0.95029
Ran for 21050 frames Cell Events: 1141 mean_circ: 0.950073
Ran for 21550 frames Cell Events: 1167 mean_circ: 0.950134
Ran for 22550 frames Cell Events: 1219 mean_circ: 0.950141
Ran for 23050 frames Cell Events: 1237 mean_circ: 0.950443
Ran for 23550 frames Cell Events: 1258 mean_circ: 0.950423
Ran for 24050 frames Cell Events: 1291 mean_circ: 0.950446
Ran for 27050 frames Cell Events: 1460 mean_circ: 0.950577
Ran for 27550 frames Cell Events: 1491 mean_circ: 0.950282
Ran for 28050 frames Cell Events: 1519 mean_circ: 0.950343
Ran for 29050 frames Cell Events: 1576 mean_circ: 0.950215
Ran for 29550 frames Cell Events: 1603 mean_circ: 0.95029
Ran for 30550 frames Cell Events: 1650 mean_circ: 0.950304
Ran for 31050 frames Cell Events: 1668 mean_circ: 0.950079
Ran for 31550 frames Cell Events: 1690 mean_circ: 0.950151
Ran for 32050 frames Cell Events: 1706 mean_circ: 0.950064
Ran for 33550 frames Cell Events: 1788 mean_circ: 0.949724
Ran for 34550 frames Cell Events: 1845 mean_circ: 0.949752
Ran for 35550 frames Cell Events: 1895 mean_circ: 0.949886
Ran for 37050 frames Cell Events: 1955 mean_circ: 0.950223
Ran for 41550 frames Cell Events: 2196 mean_circ: 0.949474
Ran for 42550 frames Cell Events: 2237 mean_circ: 0.949683
Ran for 43050 frames Cell Events: 2264 mean_circ: 0.94957
Ran for 44050 frames Cell Events: 2301 mean_circ: 0.949484
Ran for 44550 frames Cell Events: 2328 mean_circ: 0.949517
Ran for 46550 frames Cell Events: 2417 mean_circ: 0.949656
Ran for 47050 frames Cell Events: 2431 mean_circ: 0.949654
Ran for 48050 frames Cell Events: 2469 mean_circ: 0.949764
Ran for 48550 frames Cell Events: 2495 mean_circ: 0.94969
Ran for 50050 frames Cell Events: 2550 mean_circ: 0.949586
Ran for 51050 frames Cell Events: 2594 mean_circ: 0.949622
Ran for 52050 frames Cell Events: 2657 mean_circ: 0.949744
Ran for 54050 frames Cell Events: 2775 mean_circ: 0.949667
Ran for 55050 frames Cell Events: 2826 mean_circ: 0.949514
Ran for 55550 frames Cell Events: 2844 mean_circ: 0.949511
Ran for 56050 frames Cell Events: 2863 mean_circ: 0.949423
Ran for 57050 frames Cell Events: 2899 mean_circ: 0.949402
Ran for 57550 frames Cell Events: 2924 mean_circ: 0.949417
Ran for 58050 frames Cell Events: 2941 mean_circ: 0.949459
Ran for 58550 frames Cell Events: 2958 mean_circ: 0.949537
Ran for 59550 frames Cell Events: 3010 mean_circ: 0.949581
Ran for 60050 frames Cell Events: 3030 mean_circ: 0.949679
Ran for 61050 frames Cell Events: 3085 mean_circ: 0.949526
Ran for 61550 frames Cell Events: 3106 mean_circ: 0.949518
Ran for 63050 frames Cell Events: 3188 mean_circ: 0.949438
Ran for 63550 frames Cell Events: 3203 mean_circ: 0.949405
Ran for 65550 frames Cell Events: 3323 mean_circ: 0.94931
Ran for 67050 frames Cell Events: 3391 mean_circ: 0.949117
Ran for 68050 frames Cell Events: 3451 mean_circ: 0.949076
Ran for 68550 frames Cell Events: 3482 mean_circ: 0.949077
Ran for 71050 frames Cell Events: 3638 mean_circ: 0.948955
Ran for 72050 frames Cell Events: 3695 mean_circ: 0.948923
Ran for 73550 frames Cell Events: 3762 mean_circ: 0.948978
Ran for 74050 frames Cell Events: 3782 mean_circ: 0.948962
Ran for 75050 frames Cell Events: 3829 mean_circ: 0.94897
Ran for 76050 frames Cell Events: 3899 mean_circ: 0.948983
Ran for 80050 frames Cell Events: 4127 mean_circ: 0.94885
Ran for 80550 frames Cell Events: 4168 mean_circ: 0.948859
Ran for 82550 frames Cell Events: 4288 mean_circ: 0.948861
Ran for 83050 frames Cell Events: 4317 mean_circ: 0.94887
Ran for 85050 frames Cell Events: 4437 mean_circ: 0.948874
Ran for 85550 frames Cell Events: 4457 mean_circ: 0.948878
Ran for 86550 frames Cell Events: 4517 mean_circ: 0.94883
Ran for 88050 frames Cell Events: 4620 mean_circ: 0.948909
Ran for 89050 frames Cell Events: 4675 mean_circ: 0.948962
Ran for 92550 frames Cell Events: 4901 mean_circ: 0.949117
Ran for 93050 frames Cell Events: 4936 mean_circ: 0.949117
Ran for 93550 frames Cell Events: 4964 mean_circ: 0.949229
Ran for 94050 frames Cell Events: 5006 mean_circ: 0.949203
Ran for 95050 frames Cell Events: 5067 mean_circ: 0.949149
Ran for 96550 frames Cell Events: 5138 mean_circ: 0.949206
Ran for 97550 frames Cell Events: 5195 mean_circ: 0.949309
Ran for 98050 frames Cell Events: 5224 mean_circ: 0.949359
Ran for 98550 frames Cell Events: 5260 mean_circ: 0.949406
Ran for 100050 frames Cell Events: 5348 mean_circ: 0.9494
Ran for 101050 frames Cell Events: 5412 mean_circ: 0.949266
Ran for 102050 frames Cell Events: 5469 mean_circ: 0.94932
Ran for 103550 frames Cell Events: 5548 mean_circ: 0.949259
Ran for 105050 frames Cell Events: 5635 mean_circ: 0.949295
Ran for 106550 frames Cell Events: 5724 mean_circ: 0.949253
Ran for 107050 frames Cell Events: 5753 mean_circ: 0.949219
Ran for 108050 frames Cell Events: 5811 mean_circ: 0.949245
Ran for 108550 frames Cell Events: 5825 mean_circ: 0.949304
Ran for 109050 frames Cell Events: 5840 mean_circ: 0.949293
Ran for 109550 frames Cell Events: 5862 mean_circ: 0.949271
Ran for 110050 frames Cell Events: 5897 mean_circ: 0.949309
Ran for 111050 frames Cell Events: 5932 mean_circ: 0.949322
Ran for 111550 frames Cell Events: 5963 mean_circ: 0.949283
Ran for 112550 frames Cell Events: 6023 mean_circ: 0.949291
Ran for 113050 frames Cell Events: 6047 mean_circ: 0.949345
Ran for 114050 frames Cell Events: 6091 mean_circ: 0.949444
Ran for 114550 frames Cell Events: 6125 mean_circ: 0.949441
Ran for 115550 frames Cell Events: 6154 mean_circ: 0.949514
Ran for 116550 frames Cell Events: 6217 mean_circ: 0.949608
Ran for 117050 frames Cell Events: 6244 mean_circ: 0.949636
Ran for 117550 frames Cell Events: 6260 mean_circ: 0.949659
Ran for 118550 frames Cell Events: 6331 mean_circ: 0.949686
Ran for 120050 frames Cell Events: 6406 mean_circ: 0.949664
Ran for 120550 frames Cell Events: 6429 mean_circ: 0.949677
Ran for 122050 frames Cell Events: 6503 mean_circ: 0.94968
Ran for 122550 frames Cell Events: 6527 mean_circ: 0.949704
Ran for 123550 frames Cell Events: 6581 mean_circ: 0.94959
Ran for 126050 frames Cell Events: 6698 mean_circ: 0.949561
Ran for 128550 frames Cell Events: 6827 mean_circ: 0.94956
Ran for 130550 frames Cell Events: 6894 mean_circ: 0.949531
Ran for 131550 frames Cell Events: 6932 mean_circ: 0.949538
Ran for 132050 frames Cell Events: 6957 mean_circ: 0.949551
Ran for 133050 frames Cell Events: 7005 mean_circ: 0.949589
Ran for 133550 frames Cell Events: 7027 mean_circ: 0.949569
Ran for 134050 frames Cell Events: 7044 mean_circ: 0.949587
Ran for 136050 frames Cell Events: 7148 mean_circ: 0.94961
Ran for 137050 frames Cell Events: 7210 mean_circ: 0.949655
Ran for 138050 frames Cell Events: 7242 mean_circ: 0.949643
Ran for 139550 frames Cell Events: 7313 mean_circ: 0.949641
Ran for 140050 frames Cell Events: 7347 mean_circ: 0.949662
Ran for 141050 frames Cell Events: 7388 mean_circ: 0.949678
Ran for 141550 frames Cell Events: 7406 mean_circ: 0.949671
Ran for 142050 frames Cell Events: 7427 mean_circ: 0.949684
Ran for 143050 frames Cell Events: 7472 mean_circ: 0.949733
Ran for 143550 frames Cell Events: 7492 mean_circ: 0.949702
Ran for 144050 frames Cell Events: 7511 mean_circ: 0.949726
Ran for 144550 frames Cell Events: 7528 mean_circ: 0.949703
Ran for 145550 frames Cell Events: 7573 mean_circ: 0.949685
Ran for 146050 frames Cell Events: 7604 mean_circ: 0.949726
Ran for 146550 frames Cell Events: 7627 mean_circ: 0.94976
Ran for 148550 frames Cell Events: 7721 mean_circ: 0.949863
Ran for 149050 frames Cell Events: 7745 mean_circ: 0.949847
Ran for 153050 frames Cell Events: 7972 mean_circ: 0.949821
Ran for 153550 frames Cell Events: 8002 mean_circ: 0.949809
Ran for 155050 frames Cell Events: 8082 mean_circ: 0.949834
Ran for 155550 frames Cell Events: 8114 mean_circ: 0.949863
Ran for 156050 frames Cell Events: 8135 mean_circ: 0.949893
Ran for 156550 frames Cell Events: 8174 mean_circ: 0.949893
Ran for 157050 frames Cell Events: 8210 mean_circ: 0.949899
Ran for 157550 frames Cell Events: 8242 mean_circ: 0.949898
Ran for 158050 frames Cell Events: 8278 mean_circ: 0.949866
Ran for 158550 frames Cell Events: 8310 mean_circ: 0.949866
Ran for 160050 frames Cell Events: 8399 mean_circ: 0.949835
Ran for 161550 frames Cell Events: 8475 mean_circ: 0.94979
Ran for 163050 frames Cell Events: 8555 mean_circ: 0.949816
Ran for 164050 frames Cell Events: 8624 mean_circ: 0.949808



End this measurment..



Stopping time: 2.32768e+008
****************************************
Measurement finished

Mean Circularitiy: 0.949814
Mean Area: 603.105
Mean FL-1 intensity: 3432.96
Mean FL-2 intensity: 4299.4
Mean FL-3 intensity: 0

frames processed: 162796
Total Time: 82283.5ms
Number of Runs (new bg img) 0
Frames collected: 164411
Skipped frames: 73387
Rescued Frames: 6141124
Samples DAQ: 41103968
Samples written: 82205486
Samples per Channel: 41102743
FPS: 1998.1

Samples per second: 499526
Expected samples/s: 500000

Events: 8646
Events per second: 105.076
jumped back (PMT): 3760
#Frames read, frequency
# 0 frames :259572
# 1 frames :54743
# 2 frames :3579
# 3 frames :1248
# 4 frames :778
# 5 frames :428
# 6 frames :1154
# 7 frames :1418
# 8 frames :895
# 9 frames :634
# 10 frames :867
# 11 frames :451
# 12 frames :369
# 13 frames :248
# 14 frames :241
# 15 frames :227
# 16 frames :193
# 17 frames :178
# 18 frames :156
# 19 frames :127
# 20 frames :99
# 21 frames :97
# 22 frames :87
# 23 frames :60
# 24 frames :64
# 25 frames :45
# 26 frames :55
# 27 frames :47
# 28 frames :34
# 29 frames :31
# 30 frames :27
# 31 frames :19
# 32 frames :20
# 33 frames :13
# 34 frames :19
# 35 frames :10
# 36 frames :15
# 37 frames :11
# 38 frames :11
# 39 frames :11
# 40 frames :9
# 41 frames :6
# 42 frames :8
# 43 frames :6
# 44 frames :5
# 45 frames :10
# 46 frames :9
# 47 frames :6
# 48 frames :3
# 49 frames :4
# 50 frames :2
# 51 frames :2
# 52 frames :3
# 53 frames :5
# 54 frames :3
# 55 frames :2
# 56 frames :2
# 58 frames :1
# 61 frames :1
# 63 frames :1
# 64 frames :1
# 65 frames :1
# 69 frames :1
# 72 frames :1
# 78 frames :1
# 84 frames :1
# 94 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.6131
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0