Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_18.32.35 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "2ee42691eccdacd68f11567a434f156b",
"name": "M1_20170616_1951_dsRNA_Q8_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "16:32:56"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8355 (max 8356) in '8355'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.604
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.3284e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 30 mean_circ: 0.948775
Ran for 2050 frames Cell Events: 68 mean_circ: 0.951314
Ran for 2550 frames Cell Events: 83 mean_circ: 0.953147
Ran for 3050 frames Cell Events: 100 mean_circ: 0.952495
Ran for 4050 frames Cell Events: 134 mean_circ: 0.950566
Ran for 4550 frames Cell Events: 143 mean_circ: 0.950523
Ran for 5050 frames Cell Events: 155 mean_circ: 0.949748
Ran for 5550 frames Cell Events: 168 mean_circ: 0.949332
Ran for 6050 frames Cell Events: 183 mean_circ: 0.949937
Ran for 6550 frames Cell Events: 207 mean_circ: 0.950229
Ran for 7050 frames Cell Events: 229 mean_circ: 0.95018
Ran for 8050 frames Cell Events: 259 mean_circ: 0.948789
Ran for 8550 frames Cell Events: 284 mean_circ: 0.947616
Ran for 9050 frames Cell Events: 298 mean_circ: 0.947738
Ran for 10050 frames Cell Events: 323 mean_circ: 0.947801
Ran for 11050 frames Cell Events: 356 mean_circ: 0.948513
Ran for 11550 frames Cell Events: 369 mean_circ: 0.947832
Ran for 12550 frames Cell Events: 405 mean_circ: 0.946632
Ran for 13050 frames Cell Events: 424 mean_circ: 0.94658
Ran for 13550 frames Cell Events: 438 mean_circ: 0.946275
Ran for 14050 frames Cell Events: 446 mean_circ: 0.945971
Ran for 14550 frames Cell Events: 457 mean_circ: 0.946163
Ran for 15050 frames Cell Events: 472 mean_circ: 0.946571
Ran for 16050 frames Cell Events: 511 mean_circ: 0.947262
Ran for 17050 frames Cell Events: 546 mean_circ: 0.947098
Ran for 17550 frames Cell Events: 566 mean_circ: 0.946982
Ran for 19050 frames Cell Events: 637 mean_circ: 0.946839
Ran for 20550 frames Cell Events: 688 mean_circ: 0.947243
Ran for 22550 frames Cell Events: 760 mean_circ: 0.946888
Ran for 25550 frames Cell Events: 903 mean_circ: 0.94678
Ran for 27550 frames Cell Events: 964 mean_circ: 0.946443
Ran for 28050 frames Cell Events: 976 mean_circ: 0.946499
Ran for 28550 frames Cell Events: 1000 mean_circ: 0.946391
Ran for 29550 frames Cell Events: 1043 mean_circ: 0.946722
Ran for 30550 frames Cell Events: 1093 mean_circ: 0.946791
Ran for 31550 frames Cell Events: 1138 mean_circ: 0.946611
Ran for 32550 frames Cell Events: 1174 mean_circ: 0.946607
Ran for 34050 frames Cell Events: 1250 mean_circ: 0.946529
Ran for 36050 frames Cell Events: 1345 mean_circ: 0.945826
Ran for 36550 frames Cell Events: 1365 mean_circ: 0.94587
Ran for 38550 frames Cell Events: 1474 mean_circ: 0.945294
Ran for 39050 frames Cell Events: 1496 mean_circ: 0.945371
Ran for 40550 frames Cell Events: 1572 mean_circ: 0.9454
Ran for 43550 frames Cell Events: 1704 mean_circ: 0.945253
Ran for 44050 frames Cell Events: 1726 mean_circ: 0.945369
Ran for 44550 frames Cell Events: 1743 mean_circ: 0.945253
Ran for 45550 frames Cell Events: 1795 mean_circ: 0.945253
Ran for 46050 frames Cell Events: 1818 mean_circ: 0.94532
Ran for 47050 frames Cell Events: 1856 mean_circ: 0.945347
Ran for 47550 frames Cell Events: 1863 mean_circ: 0.945273
Ran for 49050 frames Cell Events: 1927 mean_circ: 0.945204
Ran for 51050 frames Cell Events: 2002 mean_circ: 0.945276
Ran for 51550 frames Cell Events: 2024 mean_circ: 0.945233
Ran for 52050 frames Cell Events: 2044 mean_circ: 0.94524
Ran for 53550 frames Cell Events: 2098 mean_circ: 0.945381
Ran for 54050 frames Cell Events: 2110 mean_circ: 0.945365
Ran for 54550 frames Cell Events: 2132 mean_circ: 0.945484
Ran for 55050 frames Cell Events: 2149 mean_circ: 0.945518
Ran for 57550 frames Cell Events: 2254 mean_circ: 0.945444
Ran for 59050 frames Cell Events: 2303 mean_circ: 0.945518
Ran for 60050 frames Cell Events: 2342 mean_circ: 0.945484
Ran for 60550 frames Cell Events: 2353 mean_circ: 0.945481
Ran for 61050 frames Cell Events: 2371 mean_circ: 0.945454
Ran for 62050 frames Cell Events: 2426 mean_circ: 0.945481
Ran for 63550 frames Cell Events: 2498 mean_circ: 0.945331
Ran for 64050 frames Cell Events: 2513 mean_circ: 0.945263
Ran for 65050 frames Cell Events: 2557 mean_circ: 0.945166
Ran for 66050 frames Cell Events: 2603 mean_circ: 0.945141
Ran for 66550 frames Cell Events: 2627 mean_circ: 0.94514
Ran for 67550 frames Cell Events: 2682 mean_circ: 0.945165
Ran for 68050 frames Cell Events: 2709 mean_circ: 0.94513
Ran for 69050 frames Cell Events: 2740 mean_circ: 0.94519
Ran for 69550 frames Cell Events: 2772 mean_circ: 0.945013
Ran for 71550 frames Cell Events: 2888 mean_circ: 0.944844
Ran for 72050 frames Cell Events: 2917 mean_circ: 0.944905
Ran for 72550 frames Cell Events: 2935 mean_circ: 0.944865
Ran for 73050 frames Cell Events: 2959 mean_circ: 0.944936
Ran for 74050 frames Cell Events: 3000 mean_circ: 0.945008
Ran for 74550 frames Cell Events: 3024 mean_circ: 0.945008
Ran for 75050 frames Cell Events: 3052 mean_circ: 0.944993
Ran for 75550 frames Cell Events: 3073 mean_circ: 0.944855
Ran for 76050 frames Cell Events: 3104 mean_circ: 0.944892
Ran for 76550 frames Cell Events: 3138 mean_circ: 0.944899
Ran for 77550 frames Cell Events: 3184 mean_circ: 0.944899
Ran for 78050 frames Cell Events: 3207 mean_circ: 0.944918
Ran for 78550 frames Cell Events: 3237 mean_circ: 0.94497
Ran for 81050 frames Cell Events: 3354 mean_circ: 0.94499
Ran for 82050 frames Cell Events: 3392 mean_circ: 0.944951
Ran for 83050 frames Cell Events: 3442 mean_circ: 0.944784
Ran for 86550 frames Cell Events: 3613 mean_circ: 0.944592
Ran for 87050 frames Cell Events: 3636 mean_circ: 0.944596
Ran for 87550 frames Cell Events: 3656 mean_circ: 0.94459
Ran for 88550 frames Cell Events: 3698 mean_circ: 0.944556
Ran for 89550 frames Cell Events: 3752 mean_circ: 0.944526
Ran for 91550 frames Cell Events: 3869 mean_circ: 0.944247
Ran for 92550 frames Cell Events: 3920 mean_circ: 0.944264
Ran for 94050 frames Cell Events: 3994 mean_circ: 0.944345
Ran for 95050 frames Cell Events: 4049 mean_circ: 0.944323
Ran for 96050 frames Cell Events: 4080 mean_circ: 0.94433
Ran for 97550 frames Cell Events: 4159 mean_circ: 0.944262
Ran for 98050 frames Cell Events: 4177 mean_circ: 0.94424
Ran for 99050 frames Cell Events: 4219 mean_circ: 0.944267
Ran for 100050 frames Cell Events: 4266 mean_circ: 0.944197
Ran for 101050 frames Cell Events: 4324 mean_circ: 0.94421
Ran for 101550 frames Cell Events: 4340 mean_circ: 0.944232
Ran for 103550 frames Cell Events: 4448 mean_circ: 0.944157
Ran for 104050 frames Cell Events: 4469 mean_circ: 0.944203
Ran for 104550 frames Cell Events: 4487 mean_circ: 0.944203
Ran for 106550 frames Cell Events: 4601 mean_circ: 0.944355
Ran for 107050 frames Cell Events: 4621 mean_circ: 0.944381
Ran for 108050 frames Cell Events: 4664 mean_circ: 0.944428
Ran for 108550 frames Cell Events: 4707 mean_circ: 0.944407
Ran for 109050 frames Cell Events: 4727 mean_circ: 0.944359
Ran for 110050 frames Cell Events: 4777 mean_circ: 0.944361
Ran for 110550 frames Cell Events: 4799 mean_circ: 0.944358
Ran for 111550 frames Cell Events: 4841 mean_circ: 0.944326
Ran for 112550 frames Cell Events: 4906 mean_circ: 0.94431
Ran for 113050 frames Cell Events: 4940 mean_circ: 0.944399
Ran for 114050 frames Cell Events: 4980 mean_circ: 0.944401
Ran for 114550 frames Cell Events: 5009 mean_circ: 0.944387
Ran for 115550 frames Cell Events: 5069 mean_circ: 0.944356
Ran for 116550 frames Cell Events: 5118 mean_circ: 0.944353
Ran for 118050 frames Cell Events: 5198 mean_circ: 0.944342
Ran for 118550 frames Cell Events: 5223 mean_circ: 0.944256
Ran for 119550 frames Cell Events: 5276 mean_circ: 0.944191
Ran for 120050 frames Cell Events: 5305 mean_circ: 0.944157
Ran for 122050 frames Cell Events: 5426 mean_circ: 0.94421
Ran for 122550 frames Cell Events: 5444 mean_circ: 0.944252
Ran for 123550 frames Cell Events: 5508 mean_circ: 0.944269
Ran for 124050 frames Cell Events: 5536 mean_circ: 0.944162
Ran for 125550 frames Cell Events: 5604 mean_circ: 0.944082
Ran for 126050 frames Cell Events: 5641 mean_circ: 0.94413
Ran for 127550 frames Cell Events: 5721 mean_circ: 0.944035
Ran for 128050 frames Cell Events: 5739 mean_circ: 0.944028
Ran for 130050 frames Cell Events: 5813 mean_circ: 0.944077
Ran for 131050 frames Cell Events: 5857 mean_circ: 0.944045
Ran for 131550 frames Cell Events: 5898 mean_circ: 0.944045
Ran for 132050 frames Cell Events: 5930 mean_circ: 0.943984
Ran for 133050 frames Cell Events: 5990 mean_circ: 0.943863
Ran for 133550 frames Cell Events: 6011 mean_circ: 0.94386
Ran for 134050 frames Cell Events: 6039 mean_circ: 0.943879
Ran for 135050 frames Cell Events: 6095 mean_circ: 0.943841
Ran for 135550 frames Cell Events: 6121 mean_circ: 0.943877
Ran for 136050 frames Cell Events: 6131 mean_circ: 0.943872
Ran for 136550 frames Cell Events: 6151 mean_circ: 0.943882
Ran for 137050 frames Cell Events: 6175 mean_circ: 0.943922
Ran for 139050 frames Cell Events: 6291 mean_circ: 0.943898
Ran for 139550 frames Cell Events: 6326 mean_circ: 0.943933
Ran for 140550 frames Cell Events: 6384 mean_circ: 0.943813
Ran for 141050 frames Cell Events: 6409 mean_circ: 0.943792
Ran for 142050 frames Cell Events: 6460 mean_circ: 0.943753
Ran for 142550 frames Cell Events: 6491 mean_circ: 0.943763
Ran for 144550 frames Cell Events: 6595 mean_circ: 0.943774
Ran for 145550 frames Cell Events: 6644 mean_circ: 0.943798
Ran for 147550 frames Cell Events: 6772 mean_circ: 0.943841
Ran for 151050 frames Cell Events: 6987 mean_circ: 0.943742
Ran for 152550 frames Cell Events: 7063 mean_circ: 0.943775
Ran for 153550 frames Cell Events: 7120 mean_circ: 0.943731
Ran for 154050 frames Cell Events: 7138 mean_circ: 0.943731
Ran for 154550 frames Cell Events: 7166 mean_circ: 0.943713
Ran for 156550 frames Cell Events: 7295 mean_circ: 0.943668
Ran for 157050 frames Cell Events: 7318 mean_circ: 0.943689
Ran for 159550 frames Cell Events: 7455 mean_circ: 0.943685
Ran for 160050 frames Cell Events: 7474 mean_circ: 0.943712
Ran for 164550 frames Cell Events: 7680 mean_circ: 0.943733
Ran for 165050 frames Cell Events: 7704 mean_circ: 0.943739
Ran for 165550 frames Cell Events: 7725 mean_circ: 0.94375
Ran for 166050 frames Cell Events: 7753 mean_circ: 0.943779
Ran for 167550 frames Cell Events: 7816 mean_circ: 0.943821
Ran for 168050 frames Cell Events: 7845 mean_circ: 0.943819
Ran for 170550 frames Cell Events: 7977 mean_circ: 0.943719
Ran for 171050 frames Cell Events: 8006 mean_circ: 0.943728
Ran for 171550 frames Cell Events: 8024 mean_circ: 0.943735
Ran for 173550 frames Cell Events: 8149 mean_circ: 0.943758
Ran for 174550 frames Cell Events: 8217 mean_circ: 0.943725
Ran for 176550 frames Cell Events: 8317 mean_circ: 0.943767



End this measurment..



Stopping time: 2.32929e+008
****************************************
Measurement finished

Mean Circularitiy: 0.943745
Mean Area: 624.574
Mean FL-1 intensity: 3304.01
Mean FL-2 intensity: 4508.62
Mean FL-3 intensity: 0

frames processed: 176343
Total Time: 88656.8ms
Number of Runs (new bg img) 0
Frames collected: 177184
Skipped frames: 74228
Rescued Frames: 6234309
Samples DAQ: 44297750
Samples written: 88592032
Samples per Channel: 44296016
FPS: 1998.54

Samples per second: 499635
Expected samples/s: 500000

Events: 8356
Events per second: 94.2511
jumped back (PMT): 22
#Frames read, frequency
# 0 frames :271013
# 1 frames :68411
# 2 frames :4011
# 3 frames :1233
# 4 frames :896
# 5 frames :405
# 6 frames :1143
# 7 frames :1635
# 8 frames :866
# 9 frames :724
# 10 frames :994
# 11 frames :451
# 12 frames :391
# 13 frames :236
# 14 frames :187
# 15 frames :227
# 16 frames :195
# 17 frames :190
# 18 frames :146
# 19 frames :94
# 20 frames :103
# 21 frames :85
# 22 frames :53
# 23 frames :69
# 24 frames :42
# 25 frames :43
# 26 frames :39
# 27 frames :31
# 28 frames :31
# 29 frames :28
# 30 frames :30
# 31 frames :16
# 32 frames :22
# 33 frames :12
# 34 frames :15
# 35 frames :11
# 36 frames :11
# 37 frames :9
# 38 frames :17
# 39 frames :10
# 40 frames :8
# 41 frames :3
# 42 frames :4
# 43 frames :4
# 44 frames :5
# 45 frames :6
# 46 frames :5
# 47 frames :1
# 48 frames :5
# 49 frames :1
# 50 frames :4
# 53 frames :1
# 54 frames :1
# 55 frames :2
# 58 frames :1
# 59 frames :1
# 61 frames :1
# 68 frames :2
# 69 frames :1
# 71 frames :1
# 84 frames :1
# 86 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.604
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0