Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_18.33.30 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "c5da4145f6d3c1529a763facd49f4540",
"name": "M1_20170616_2002_dsRNA_p6_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "16:33:51"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8197 (max 8198) in '8197'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.5313
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.33493e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1550 frames Cell Events: 102 mean_circ: 0.946044
Ran for 2050 frames Cell Events: 128 mean_circ: 0.94686
Ran for 3050 frames Cell Events: 178 mean_circ: 0.945462
Ran for 4550 frames Cell Events: 242 mean_circ: 0.947716
Ran for 6550 frames Cell Events: 375 mean_circ: 0.945205
Ran for 7050 frames Cell Events: 402 mean_circ: 0.945112
Ran for 8050 frames Cell Events: 456 mean_circ: 0.944348
Ran for 9050 frames Cell Events: 505 mean_circ: 0.943568
Ran for 10050 frames Cell Events: 579 mean_circ: 0.943452
Ran for 10550 frames Cell Events: 601 mean_circ: 0.942976
Ran for 11550 frames Cell Events: 651 mean_circ: 0.943415
Ran for 12050 frames Cell Events: 678 mean_circ: 0.943263
Ran for 12550 frames Cell Events: 693 mean_circ: 0.94329
Ran for 13050 frames Cell Events: 708 mean_circ: 0.943352
Ran for 14550 frames Cell Events: 799 mean_circ: 0.943693
Ran for 16050 frames Cell Events: 867 mean_circ: 0.94369
Ran for 16550 frames Cell Events: 889 mean_circ: 0.943284
Ran for 17050 frames Cell Events: 908 mean_circ: 0.94307
Ran for 18550 frames Cell Events: 996 mean_circ: 0.942956
Ran for 20050 frames Cell Events: 1066 mean_circ: 0.942785
Ran for 21050 frames Cell Events: 1117 mean_circ: 0.942888
Ran for 24050 frames Cell Events: 1304 mean_circ: 0.942914
Ran for 24550 frames Cell Events: 1327 mean_circ: 0.942728
Ran for 25050 frames Cell Events: 1350 mean_circ: 0.942607
Ran for 25550 frames Cell Events: 1379 mean_circ: 0.942807
Ran for 26050 frames Cell Events: 1420 mean_circ: 0.942803
Ran for 26550 frames Cell Events: 1450 mean_circ: 0.942391
Ran for 27050 frames Cell Events: 1466 mean_circ: 0.942146
Ran for 28050 frames Cell Events: 1520 mean_circ: 0.941677
Ran for 29050 frames Cell Events: 1580 mean_circ: 0.941818
Ran for 29550 frames Cell Events: 1615 mean_circ: 0.941593
Ran for 30550 frames Cell Events: 1661 mean_circ: 0.941614
Ran for 31050 frames Cell Events: 1683 mean_circ: 0.941505
Ran for 31550 frames Cell Events: 1706 mean_circ: 0.941336
Ran for 32050 frames Cell Events: 1724 mean_circ: 0.941422
Ran for 34050 frames Cell Events: 1839 mean_circ: 0.941213
Ran for 36050 frames Cell Events: 1983 mean_circ: 0.941
Ran for 37050 frames Cell Events: 2015 mean_circ: 0.941116
Ran for 38050 frames Cell Events: 2076 mean_circ: 0.941172
Ran for 41550 frames Cell Events: 2307 mean_circ: 0.94144
Ran for 42550 frames Cell Events: 2398 mean_circ: 0.941396
Ran for 43050 frames Cell Events: 2429 mean_circ: 0.941393
Ran for 44550 frames Cell Events: 2522 mean_circ: 0.941271
Ran for 45050 frames Cell Events: 2545 mean_circ: 0.941295
Ran for 45550 frames Cell Events: 2571 mean_circ: 0.941237
Ran for 46550 frames Cell Events: 2622 mean_circ: 0.941122
Ran for 47050 frames Cell Events: 2643 mean_circ: 0.94118
Ran for 48050 frames Cell Events: 2696 mean_circ: 0.941265
Ran for 49550 frames Cell Events: 2789 mean_circ: 0.941178
Ran for 50050 frames Cell Events: 2811 mean_circ: 0.941221
Ran for 50550 frames Cell Events: 2832 mean_circ: 0.941221
Ran for 51050 frames Cell Events: 2858 mean_circ: 0.941165
Ran for 51550 frames Cell Events: 2878 mean_circ: 0.940976
Ran for 52050 frames Cell Events: 2907 mean_circ: 0.940976
Ran for 54550 frames Cell Events: 3023 mean_circ: 0.940746
Ran for 55050 frames Cell Events: 3042 mean_circ: 0.940715
Ran for 56050 frames Cell Events: 3084 mean_circ: 0.940665
Ran for 57050 frames Cell Events: 3137 mean_circ: 0.940655
Ran for 59550 frames Cell Events: 3254 mean_circ: 0.940764
Ran for 60050 frames Cell Events: 3282 mean_circ: 0.940761
Ran for 60550 frames Cell Events: 3294 mean_circ: 0.940773
Ran for 62050 frames Cell Events: 3363 mean_circ: 0.940765
Ran for 62550 frames Cell Events: 3384 mean_circ: 0.940786
Ran for 63050 frames Cell Events: 3406 mean_circ: 0.940762
Ran for 63550 frames Cell Events: 3442 mean_circ: 0.940794
Ran for 65050 frames Cell Events: 3538 mean_circ: 0.940411
Ran for 65550 frames Cell Events: 3563 mean_circ: 0.940392
Ran for 66050 frames Cell Events: 3589 mean_circ: 0.94041
Ran for 66550 frames Cell Events: 3614 mean_circ: 0.940397
Ran for 67550 frames Cell Events: 3675 mean_circ: 0.940412
Ran for 68550 frames Cell Events: 3739 mean_circ: 0.940338
Ran for 69550 frames Cell Events: 3806 mean_circ: 0.940418
Ran for 70050 frames Cell Events: 3821 mean_circ: 0.940395
Ran for 70550 frames Cell Events: 3846 mean_circ: 0.940387
Ran for 71050 frames Cell Events: 3884 mean_circ: 0.940237
Ran for 72050 frames Cell Events: 3940 mean_circ: 0.940344
Ran for 73050 frames Cell Events: 4015 mean_circ: 0.940253
Ran for 74050 frames Cell Events: 4072 mean_circ: 0.940243
Ran for 74550 frames Cell Events: 4111 mean_circ: 0.940311
Ran for 75050 frames Cell Events: 4140 mean_circ: 0.940334
Ran for 75550 frames Cell Events: 4173 mean_circ: 0.940279
Ran for 76050 frames Cell Events: 4205 mean_circ: 0.940163
Ran for 77550 frames Cell Events: 4295 mean_circ: 0.940239
Ran for 78050 frames Cell Events: 4343 mean_circ: 0.940155
Ran for 78550 frames Cell Events: 4384 mean_circ: 0.940167
Ran for 80050 frames Cell Events: 4478 mean_circ: 0.939963
Ran for 80550 frames Cell Events: 4499 mean_circ: 0.939992
Ran for 81050 frames Cell Events: 4530 mean_circ: 0.939972
Ran for 82050 frames Cell Events: 4596 mean_circ: 0.940019
Ran for 82550 frames Cell Events: 4623 mean_circ: 0.940077
Ran for 83050 frames Cell Events: 4644 mean_circ: 0.940022
Ran for 83550 frames Cell Events: 4684 mean_circ: 0.94001
Ran for 84050 frames Cell Events: 4715 mean_circ: 0.939979
Ran for 86050 frames Cell Events: 4827 mean_circ: 0.940053
Ran for 86550 frames Cell Events: 4858 mean_circ: 0.940091
Ran for 87550 frames Cell Events: 4923 mean_circ: 0.939954
Ran for 88050 frames Cell Events: 4958 mean_circ: 0.93991
Ran for 89050 frames Cell Events: 5026 mean_circ: 0.939943
Ran for 92550 frames Cell Events: 5260 mean_circ: 0.93965
Ran for 94050 frames Cell Events: 5346 mean_circ: 0.939625
Ran for 94550 frames Cell Events: 5399 mean_circ: 0.939625
Ran for 96550 frames Cell Events: 5546 mean_circ: 0.93959
Ran for 98050 frames Cell Events: 5661 mean_circ: 0.939568
Ran for 98550 frames Cell Events: 5683 mean_circ: 0.939606
Ran for 99550 frames Cell Events: 5732 mean_circ: 0.939588
Ran for 100050 frames Cell Events: 5758 mean_circ: 0.939613
Ran for 100550 frames Cell Events: 5790 mean_circ: 0.939615
Ran for 101550 frames Cell Events: 5861 mean_circ: 0.939537
Ran for 104550 frames Cell Events: 6006 mean_circ: 0.939458
Ran for 105050 frames Cell Events: 6030 mean_circ: 0.939435
Ran for 105550 frames Cell Events: 6059 mean_circ: 0.939396
Ran for 106550 frames Cell Events: 6115 mean_circ: 0.939411
Ran for 107050 frames Cell Events: 6137 mean_circ: 0.939417
Ran for 111550 frames Cell Events: 6429 mean_circ: 0.939441
Ran for 113050 frames Cell Events: 6539 mean_circ: 0.939466
Ran for 114050 frames Cell Events: 6623 mean_circ: 0.939516
Ran for 116050 frames Cell Events: 6747 mean_circ: 0.939476
Ran for 118050 frames Cell Events: 6879 mean_circ: 0.939512
Ran for 122550 frames Cell Events: 7185 mean_circ: 0.939465
Ran for 123050 frames Cell Events: 7222 mean_circ: 0.939465
Ran for 123550 frames Cell Events: 7237 mean_circ: 0.939426
Ran for 124550 frames Cell Events: 7284 mean_circ: 0.939433
Ran for 125050 frames Cell Events: 7318 mean_circ: 0.939457
Ran for 125550 frames Cell Events: 7347 mean_circ: 0.939443
Ran for 126050 frames Cell Events: 7389 mean_circ: 0.93942
Ran for 127050 frames Cell Events: 7464 mean_circ: 0.939403
Ran for 127550 frames Cell Events: 7502 mean_circ: 0.939326
Ran for 129050 frames Cell Events: 7594 mean_circ: 0.939276
Ran for 129550 frames Cell Events: 7624 mean_circ: 0.939229
Ran for 130550 frames Cell Events: 7685 mean_circ: 0.939232
Ran for 132550 frames Cell Events: 7828 mean_circ: 0.93905
Ran for 135050 frames Cell Events: 7992 mean_circ: 0.938961
Ran for 137050 frames Cell Events: 8096 mean_circ: 0.938968
Ran for 138050 frames Cell Events: 8147 mean_circ: 0.938967



End this measurment..



Stopping time: 2.33563e+008
****************************************
Measurement finished

Mean Circularitiy: 0.938941
Mean Area: 597.282
Mean FL-1 intensity: 3061.33
Mean FL-2 intensity: 4021.45
Mean FL-3 intensity: 0

frames processed: 135592
Total Time: 69638ms
Number of Runs (new bg img) 0
Frames collected: 139133
Skipped frames: 82174
Rescued Frames: 6391972
Samples DAQ: 34784564
Samples written: 69566410
Samples per Channel: 34783205
FPS: 1997.95

Samples per second: 499486
Expected samples/s: 500000

Events: 8198
Events per second: 117.723
jumped back (PMT): 28
#Frames read, frequency
# 0 frames :217895
# 1 frames :48492
# 2 frames :2599
# 3 frames :953
# 4 frames :518
# 5 frames :283
# 6 frames :2091
# 7 frames :1025
# 8 frames :880
# 9 frames :525
# 10 frames :440
# 11 frames :331
# 12 frames :238
# 13 frames :206
# 14 frames :254
# 15 frames :168
# 16 frames :145
# 17 frames :119
# 18 frames :79
# 19 frames :84
# 20 frames :88
# 21 frames :68
# 22 frames :59
# 23 frames :53
# 24 frames :37
# 25 frames :32
# 26 frames :35
# 27 frames :28
# 28 frames :31
# 29 frames :28
# 30 frames :14
# 31 frames :19
# 32 frames :25
# 33 frames :18
# 34 frames :13
# 35 frames :13
# 36 frames :9
# 37 frames :10
# 38 frames :10
# 39 frames :6
# 40 frames :7
# 41 frames :5
# 42 frames :4
# 43 frames :2
# 44 frames :4
# 45 frames :5
# 46 frames :3
# 47 frames :4
# 48 frames :2
# 49 frames :1
# 50 frames :2
# 51 frames :1
# 52 frames :2
# 53 frames :1
# 55 frames :4
# 56 frames :3
# 59 frames :1
# 64 frames :1
# 65 frames :3
# 66 frames :1
# 72 frames :1
# 74 frames :1
# 77 frames :1
# 88 frames :1
# 91 frames :1
# 92 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.5313
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0