Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_18.36.23 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "95766eed1a6a66588ef2fe189e4946b8",
"name": "M1_20170616_2041_set1_dsRNA_26b_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "16:36:48"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 10001 (max 10002) in
'10001'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.8296
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.3585e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 30 mean_circ: 0.939804
Ran for 2050 frames Cell Events: 58 mean_circ: 0.934253
Ran for 3050 frames Cell Events: 90 mean_circ: 0.930049
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Ran for 5550 frames Cell Events: 163 mean_circ: 0.932651
Ran for 7050 frames Cell Events: 220 mean_circ: 0.92884
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Ran for 8050 frames Cell Events: 238 mean_circ: 0.92994
Ran for 9050 frames Cell Events: 267 mean_circ: 0.930165
Ran for 9550 frames Cell Events: 292 mean_circ: 0.929838
Ran for 10050 frames Cell Events: 313 mean_circ: 0.929939
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Ran for 11050 frames Cell Events: 344 mean_circ: 0.928643
Ran for 12550 frames Cell Events: 411 mean_circ: 0.925653
Ran for 13050 frames Cell Events: 421 mean_circ: 0.925437
Ran for 14050 frames Cell Events: 454 mean_circ: 0.924707
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Ran for 81050 frames Cell Events: 3100 mean_circ: 0.925365
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Ran for 82550 frames Cell Events: 3172 mean_circ: 0.925563
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Ran for 90550 frames Cell Events: 3543 mean_circ: 0.925343
Ran for 91050 frames Cell Events: 3558 mean_circ: 0.925288
Ran for 91550 frames Cell Events: 3575 mean_circ: 0.925344
Ran for 92550 frames Cell Events: 3610 mean_circ: 0.925143
Ran for 93050 frames Cell Events: 3621 mean_circ: 0.925175
Ran for 94050 frames Cell Events: 3668 mean_circ: 0.925113
Ran for 94550 frames Cell Events: 3685 mean_circ: 0.925255
Ran for 96050 frames Cell Events: 3724 mean_circ: 0.925263
Ran for 96550 frames Cell Events: 3737 mean_circ: 0.92525
Ran for 97050 frames Cell Events: 3755 mean_circ: 0.925196
Ran for 98050 frames Cell Events: 3771 mean_circ: 0.925183
Ran for 99550 frames Cell Events: 3827 mean_circ: 0.925201
Ran for 100550 frames Cell Events: 3867 mean_circ: 0.925125
Ran for 102550 frames Cell Events: 3950 mean_circ: 0.925083
Ran for 103050 frames Cell Events: 3965 mean_circ: 0.925141
Ran for 104050 frames Cell Events: 4008 mean_circ: 0.925326
Ran for 106550 frames Cell Events: 4126 mean_circ: 0.925226
Ran for 107050 frames Cell Events: 4141 mean_circ: 0.925232
Ran for 108050 frames Cell Events: 4164 mean_circ: 0.925245
Ran for 109050 frames Cell Events: 4215 mean_circ: 0.925247
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Ran for 110550 frames Cell Events: 4279 mean_circ: 0.92529
Ran for 111550 frames Cell Events: 4323 mean_circ: 0.925332
Ran for 112050 frames Cell Events: 4343 mean_circ: 0.925297
Ran for 112550 frames Cell Events: 4354 mean_circ: 0.925331
Ran for 113050 frames Cell Events: 4366 mean_circ: 0.925374
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Ran for 121050 frames Cell Events: 4669 mean_circ: 0.925507
Ran for 123550 frames Cell Events: 4793 mean_circ: 0.92571
Ran for 124050 frames Cell Events: 4828 mean_circ: 0.925758
Ran for 125050 frames Cell Events: 4858 mean_circ: 0.925725
Ran for 126550 frames Cell Events: 4905 mean_circ: 0.925761
Ran for 128050 frames Cell Events: 4953 mean_circ: 0.925686
Ran for 128550 frames Cell Events: 4971 mean_circ: 0.925676
Ran for 129050 frames Cell Events: 4986 mean_circ: 0.925628
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Ran for 130550 frames Cell Events: 5035 mean_circ: 0.925575
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Ran for 132050 frames Cell Events: 5088 mean_circ: 0.92559
Ran for 132550 frames Cell Events: 5101 mean_circ: 0.925531
Ran for 133050 frames Cell Events: 5115 mean_circ: 0.925499
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Ran for 134050 frames Cell Events: 5156 mean_circ: 0.925507
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Ran for 136050 frames Cell Events: 5244 mean_circ: 0.925286
Ran for 136550 frames Cell Events: 5264 mean_circ: 0.925249
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Ran for 139050 frames Cell Events: 5343 mean_circ: 0.925225
Ran for 140550 frames Cell Events: 5409 mean_circ: 0.925282
Ran for 141550 frames Cell Events: 5445 mean_circ: 0.925241
Ran for 142050 frames Cell Events: 5459 mean_circ: 0.925301
Ran for 143050 frames Cell Events: 5521 mean_circ: 0.925349
Ran for 144050 frames Cell Events: 5567 mean_circ: 0.92535
Ran for 144550 frames Cell Events: 5578 mean_circ: 0.925367
Ran for 145050 frames Cell Events: 5598 mean_circ: 0.925326
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Ran for 146550 frames Cell Events: 5677 mean_circ: 0.925357
Ran for 147050 frames Cell Events: 5683 mean_circ: 0.925386
Ran for 147550 frames Cell Events: 5702 mean_circ: 0.92534
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Ran for 151550 frames Cell Events: 5879 mean_circ: 0.925222
Ran for 152550 frames Cell Events: 5930 mean_circ: 0.925202
Ran for 153050 frames Cell Events: 5945 mean_circ: 0.925171
Ran for 153550 frames Cell Events: 5970 mean_circ: 0.925164
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Ran for 156550 frames Cell Events: 6097 mean_circ: 0.92504
Ran for 158050 frames Cell Events: 6167 mean_circ: 0.925072
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Ran for 161550 frames Cell Events: 6316 mean_circ: 0.925139
Ran for 162550 frames Cell Events: 6354 mean_circ: 0.925152
Ran for 163050 frames Cell Events: 6375 mean_circ: 0.925065
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Ran for 166050 frames Cell Events: 6512 mean_circ: 0.925109
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Ran for 202050 frames Cell Events: 8117 mean_circ: 0.924872
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Ran for 206550 frames Cell Events: 8352 mean_circ: 0.924772
Ran for 207050 frames Cell Events: 8372 mean_circ: 0.924765
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Ran for 208050 frames Cell Events: 8437 mean_circ: 0.924742
Ran for 208550 frames Cell Events: 8460 mean_circ: 0.924783
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Ran for 211050 frames Cell Events: 8551 mean_circ: 0.924758
Ran for 211550 frames Cell Events: 8576 mean_circ: 0.924764
Ran for 213050 frames Cell Events: 8649 mean_circ: 0.924781
Ran for 213550 frames Cell Events: 8673 mean_circ: 0.924746
Ran for 214050 frames Cell Events: 8706 mean_circ: 0.924717
Ran for 214550 frames Cell Events: 8727 mean_circ: 0.924726
Ran for 219050 frames Cell Events: 8956 mean_circ: 0.924683
Ran for 220550 frames Cell Events: 9026 mean_circ: 0.92469
Ran for 221050 frames Cell Events: 9048 mean_circ: 0.924696
Ran for 221550 frames Cell Events: 9066 mean_circ: 0.924738
Ran for 223050 frames Cell Events: 9103 mean_circ: 0.924771
Ran for 223550 frames Cell Events: 9125 mean_circ: 0.924757
Ran for 224050 frames Cell Events: 9162 mean_circ: 0.924771
Ran for 226050 frames Cell Events: 9275 mean_circ: 0.924797
Ran for 226550 frames Cell Events: 9297 mean_circ: 0.924825
Ran for 227050 frames Cell Events: 9316 mean_circ: 0.924792
Ran for 227550 frames Cell Events: 9332 mean_circ: 0.924689
Ran for 228050 frames Cell Events: 9357 mean_circ: 0.924687
Ran for 228550 frames Cell Events: 9385 mean_circ: 0.924621
Ran for 229050 frames Cell Events: 9409 mean_circ: 0.924653
Ran for 229550 frames Cell Events: 9435 mean_circ: 0.924684
Ran for 230050 frames Cell Events: 9459 mean_circ: 0.924672
Ran for 231050 frames Cell Events: 9510 mean_circ: 0.924626
Ran for 231550 frames Cell Events: 9533 mean_circ: 0.924616
Ran for 232050 frames Cell Events: 9552 mean_circ: 0.924582
Ran for 233550 frames Cell Events: 9617 mean_circ: 0.924608
Ran for 234050 frames Cell Events: 9633 mean_circ: 0.924602
Ran for 234550 frames Cell Events: 9663 mean_circ: 0.92451
Ran for 235050 frames Cell Events: 9686 mean_circ: 0.924468
Ran for 236050 frames Cell Events: 9727 mean_circ: 0.924468
Ran for 237050 frames Cell Events: 9768 mean_circ: 0.924497
Ran for 238050 frames Cell Events: 9816 mean_circ: 0.924451
Ran for 239050 frames Cell Events: 9850 mean_circ: 0.924486
Ran for 240050 frames Cell Events: 9902 mean_circ: 0.924479
Ran for 241050 frames Cell Events: 9930 mean_circ: 0.924498
Ran for 241550 frames Cell Events: 9950 mean_circ: 0.924488
Ran for 242050 frames Cell Events: 9970 mean_circ: 0.92451
Ran for 242550 frames Cell Events: 9986 mean_circ: 0.924507



End this measurment.
because stop condition (time/nr of cells) was met

Stopping time: 2.35972e+008
****************************************
Measurement finished

Mean Circularitiy: 0.924535
Mean Area: 801.37
Mean FL-1 intensity: 10303
Mean FL-2 intensity: 13383.9
Mean FL-3 intensity: 0

frames processed: 241757
Total Time: 121520ms
Number of Runs (new bg img) 0
Frames collected: 242907
Skipped frames: 91750
Rescued Frames: 7004906
Samples DAQ: 60730258
Samples written: 121453306
Samples per Channel: 60726653
FPS: 1998.91

Samples per second: 499726
Expected samples/s: 500000

Events: 10002
Events per second: 82.3076
jumped back (PMT): 10
#Frames read, frequency
# 0 frames :336920
# 1 frames :129564
# 2 frames :5766
# 3 frames :1625
# 4 frames :718
# 5 frames :254
# 6 frames :3528
# 7 frames :2504
# 8 frames :1798
# 9 frames :718
# 10 frames :420
# 11 frames :350
# 12 frames :351
# 13 frames :197
# 14 frames :245
# 15 frames :180
# 16 frames :124
# 17 frames :79
# 18 frames :69
# 19 frames :47
# 20 frames :42
# 21 frames :36
# 22 frames :26
# 23 frames :18
# 24 frames :18
# 25 frames :17
# 26 frames :10
# 27 frames :14
# 28 frames :6
# 29 frames :6
# 30 frames :8
# 31 frames :5
# 32 frames :4
# 33 frames :6
# 34 frames :4
# 35 frames :1
# 36 frames :1
# 38 frames :1
# 39 frames :1
# 40 frames :2
# 42 frames :1
# 44 frames :1
# 48 frames :1
# 49 frames :1
# 51 frames :1
# 56 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.8296
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0