Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_19.03.00 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "c82515699ed7211d0b6b67c42384d8ff",
"name": "M1_20170618_1920_set1_dsRNA_25b_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "17:03:20"
}
}
dclab-tdms2rtdc-warnings MultipleSamplesPerEventFound
in dclab.rtdc_dataset.fmt_tdms.exc line 101:
Ignoring trace data of
'\\syn03.frontend.storage.int.mpl.mpg.de\guck_division2\Members\Laura\20240219_Kasia_screen_all_folders\20170618_katarzyna_kc167_phospho_screen2\20170618_1920_set1_dsRNA_25b_col\M1_20170618_1920_set1_dsRNA_25b_col_traces.tdms'
due to multiple values for samples per event: [300 600]
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8032 (max 8033) in '8032'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0081
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 4.03786e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 1 mean_circ: 0.938151
Ran for 1550 frames Cell Events: 36 mean_circ: 0.929215
Ran for 2050 frames Cell Events: 48 mean_circ: 0.932226
Ran for 3050 frames Cell Events: 84 mean_circ: 0.935171
Ran for 3550 frames Cell Events: 93 mean_circ: 0.932968
Ran for 4050 frames Cell Events: 109 mean_circ: 0.93296
Ran for 4550 frames Cell Events: 121 mean_circ: 0.93079
Ran for 5550 frames Cell Events: 147 mean_circ: 0.930464
Ran for 6050 frames Cell Events: 159 mean_circ: 0.933309
Ran for 7550 frames Cell Events: 199 mean_circ: 0.935181
Ran for 8050 frames Cell Events: 209 mean_circ: 0.934934
Ran for 8550 frames Cell Events: 214 mean_circ: 0.935211
Ran for 9050 frames Cell Events: 226 mean_circ: 0.935128
Ran for 9550 frames Cell Events: 241 mean_circ: 0.934852
Ran for 10050 frames Cell Events: 252 mean_circ: 0.935186
Ran for 10550 frames Cell Events: 267 mean_circ: 0.934461
Ran for 12550 frames Cell Events: 328 mean_circ: 0.933075
Ran for 13550 frames Cell Events: 346 mean_circ: 0.933167
Ran for 14050 frames Cell Events: 357 mean_circ: 0.932995
Ran for 14550 frames Cell Events: 360 mean_circ: 0.932922
Ran for 15050 frames Cell Events: 381 mean_circ: 0.933573
Ran for 15550 frames Cell Events: 395 mean_circ: 0.933831
Ran for 16050 frames Cell Events: 408 mean_circ: 0.933787
Ran for 16550 frames Cell Events: 417 mean_circ: 0.934622
Ran for 17050 frames Cell Events: 428 mean_circ: 0.935142
Ran for 18050 frames Cell Events: 463 mean_circ: 0.935716
Ran for 18550 frames Cell Events: 473 mean_circ: 0.935658
Ran for 19050 frames Cell Events: 487 mean_circ: 0.935568
Ran for 19550 frames Cell Events: 501 mean_circ: 0.93549
Ran for 20050 frames Cell Events: 513 mean_circ: 0.93597
Ran for 20550 frames Cell Events: 533 mean_circ: 0.935964
Ran for 21050 frames Cell Events: 540 mean_circ: 0.935881
Ran for 22050 frames Cell Events: 564 mean_circ: 0.936142
Ran for 22550 frames Cell Events: 579 mean_circ: 0.936265
Ran for 23050 frames Cell Events: 593 mean_circ: 0.936637
Ran for 23550 frames Cell Events: 609 mean_circ: 0.936556
Ran for 25550 frames Cell Events: 655 mean_circ: 0.935565
Ran for 26050 frames Cell Events: 664 mean_circ: 0.935735
Ran for 26550 frames Cell Events: 675 mean_circ: 0.935841
Ran for 27050 frames Cell Events: 679 mean_circ: 0.936015
Ran for 27550 frames Cell Events: 685 mean_circ: 0.936107
Ran for 28550 frames Cell Events: 718 mean_circ: 0.936292
Ran for 29050 frames Cell Events: 745 mean_circ: 0.936429
Ran for 29550 frames Cell Events: 758 mean_circ: 0.936246
Ran for 30550 frames Cell Events: 793 mean_circ: 0.936305
Ran for 31050 frames Cell Events: 809 mean_circ: 0.936202
Ran for 31550 frames Cell Events: 820 mean_circ: 0.936064
Ran for 32550 frames Cell Events: 856 mean_circ: 0.935505
Ran for 33050 frames Cell Events: 868 mean_circ: 0.93549
Ran for 33550 frames Cell Events: 880 mean_circ: 0.935707
Ran for 34050 frames Cell Events: 889 mean_circ: 0.935183
Ran for 35050 frames Cell Events: 919 mean_circ: 0.935393
Ran for 35550 frames Cell Events: 933 mean_circ: 0.935466
Ran for 36050 frames Cell Events: 940 mean_circ: 0.935519
Ran for 36550 frames Cell Events: 960 mean_circ: 0.935616
Ran for 37050 frames Cell Events: 976 mean_circ: 0.9355
Ran for 37550 frames Cell Events: 998 mean_circ: 0.935344
Ran for 39050 frames Cell Events: 1030 mean_circ: 0.935196
Ran for 39550 frames Cell Events: 1047 mean_circ: 0.935236
Ran for 40050 frames Cell Events: 1059 mean_circ: 0.935187
Ran for 40550 frames Cell Events: 1074 mean_circ: 0.93506
Ran for 41050 frames Cell Events: 1082 mean_circ: 0.93506
Ran for 41550 frames Cell Events: 1102 mean_circ: 0.93499
Ran for 42050 frames Cell Events: 1120 mean_circ: 0.934843
Ran for 42550 frames Cell Events: 1136 mean_circ: 0.934704
Ran for 43050 frames Cell Events: 1148 mean_circ: 0.934871
Ran for 43550 frames Cell Events: 1168 mean_circ: 0.934616
Ran for 44050 frames Cell Events: 1184 mean_circ: 0.934398
Ran for 44550 frames Cell Events: 1200 mean_circ: 0.934316
Ran for 45050 frames Cell Events: 1210 mean_circ: 0.93448
Ran for 45550 frames Cell Events: 1229 mean_circ: 0.934704
Ran for 46050 frames Cell Events: 1242 mean_circ: 0.934666
Ran for 47050 frames Cell Events: 1268 mean_circ: 0.934446
Ran for 47550 frames Cell Events: 1276 mean_circ: 0.934474
Ran for 48050 frames Cell Events: 1293 mean_circ: 0.934652
Ran for 48550 frames Cell Events: 1310 mean_circ: 0.934592
Ran for 49050 frames Cell Events: 1329 mean_circ: 0.934781
Ran for 50550 frames Cell Events: 1355 mean_circ: 0.934862
Ran for 51050 frames Cell Events: 1364 mean_circ: 0.934898
Ran for 51550 frames Cell Events: 1375 mean_circ: 0.93489
Ran for 52550 frames Cell Events: 1409 mean_circ: 0.934721
Ran for 53050 frames Cell Events: 1417 mean_circ: 0.934809
Ran for 54050 frames Cell Events: 1449 mean_circ: 0.934498
Ran for 54550 frames Cell Events: 1462 mean_circ: 0.93447
Ran for 55050 frames Cell Events: 1471 mean_circ: 0.93443
Ran for 55550 frames Cell Events: 1492 mean_circ: 0.934314
Ran for 56050 frames Cell Events: 1511 mean_circ: 0.934398
Ran for 56550 frames Cell Events: 1525 mean_circ: 0.934335
Ran for 57050 frames Cell Events: 1532 mean_circ: 0.934238
Ran for 57550 frames Cell Events: 1539 mean_circ: 0.934204
Ran for 58550 frames Cell Events: 1583 mean_circ: 0.934011
Ran for 59050 frames Cell Events: 1602 mean_circ: 0.933991
Ran for 59550 frames Cell Events: 1622 mean_circ: 0.934
Ran for 60050 frames Cell Events: 1630 mean_circ: 0.933804
Ran for 60550 frames Cell Events: 1640 mean_circ: 0.933541
Ran for 61550 frames Cell Events: 1659 mean_circ: 0.933743
Ran for 62050 frames Cell Events: 1667 mean_circ: 0.933862
Ran for 62550 frames Cell Events: 1676 mean_circ: 0.933924
Ran for 63050 frames Cell Events: 1687 mean_circ: 0.934097
Ran for 64050 frames Cell Events: 1717 mean_circ: 0.934103
Ran for 64550 frames Cell Events: 1727 mean_circ: 0.934012
Ran for 65050 frames Cell Events: 1743 mean_circ: 0.93413
Ran for 66050 frames Cell Events: 1775 mean_circ: 0.934164
Ran for 67550 frames Cell Events: 1826 mean_circ: 0.934634
Ran for 68050 frames Cell Events: 1846 mean_circ: 0.93466
Ran for 69050 frames Cell Events: 1906 mean_circ: 0.934737
Ran for 69550 frames Cell Events: 1919 mean_circ: 0.934759
Ran for 70550 frames Cell Events: 1942 mean_circ: 0.934856
Ran for 71550 frames Cell Events: 1973 mean_circ: 0.934756
Ran for 72050 frames Cell Events: 1987 mean_circ: 0.93482
Ran for 72550 frames Cell Events: 1996 mean_circ: 0.934842
Ran for 74050 frames Cell Events: 2033 mean_circ: 0.935065
Ran for 75050 frames Cell Events: 2071 mean_circ: 0.934984
Ran for 75550 frames Cell Events: 2105 mean_circ: 0.934818
Ran for 76050 frames Cell Events: 2118 mean_circ: 0.934948
Ran for 77050 frames Cell Events: 2146 mean_circ: 0.934858
Ran for 78050 frames Cell Events: 2180 mean_circ: 0.934793
Ran for 78550 frames Cell Events: 2202 mean_circ: 0.934725
Ran for 79050 frames Cell Events: 2221 mean_circ: 0.934686
Ran for 80050 frames Cell Events: 2256 mean_circ: 0.9349
Ran for 80550 frames Cell Events: 2268 mean_circ: 0.934845
Ran for 81550 frames Cell Events: 2299 mean_circ: 0.934825
Ran for 82050 frames Cell Events: 2311 mean_circ: 0.934768
Ran for 83050 frames Cell Events: 2347 mean_circ: 0.934694
Ran for 83550 frames Cell Events: 2359 mean_circ: 0.934735
Ran for 84050 frames Cell Events: 2380 mean_circ: 0.93486
Ran for 84550 frames Cell Events: 2396 mean_circ: 0.934865
Ran for 85050 frames Cell Events: 2413 mean_circ: 0.934945
Ran for 86050 frames Cell Events: 2462 mean_circ: 0.935073
Ran for 87550 frames Cell Events: 2501 mean_circ: 0.935028
Ran for 88050 frames Cell Events: 2515 mean_circ: 0.935054
Ran for 88550 frames Cell Events: 2531 mean_circ: 0.935093
Ran for 89050 frames Cell Events: 2542 mean_circ: 0.935001
Ran for 90550 frames Cell Events: 2581 mean_circ: 0.935031
Ran for 91050 frames Cell Events: 2591 mean_circ: 0.934999
Ran for 91550 frames Cell Events: 2605 mean_circ: 0.934965
Ran for 92550 frames Cell Events: 2640 mean_circ: 0.935
Ran for 93050 frames Cell Events: 2649 mean_circ: 0.935
Ran for 93550 frames Cell Events: 2667 mean_circ: 0.934914
Ran for 94050 frames Cell Events: 2682 mean_circ: 0.934946
Ran for 95050 frames Cell Events: 2713 mean_circ: 0.934805
Ran for 95550 frames Cell Events: 2722 mean_circ: 0.934818
Ran for 96050 frames Cell Events: 2734 mean_circ: 0.93479
Ran for 97050 frames Cell Events: 2765 mean_circ: 0.934897
Ran for 97550 frames Cell Events: 2776 mean_circ: 0.934931
Ran for 98050 frames Cell Events: 2785 mean_circ: 0.934947
Ran for 98550 frames Cell Events: 2801 mean_circ: 0.934908
Ran for 99050 frames Cell Events: 2811 mean_circ: 0.934803
Ran for 99550 frames Cell Events: 2829 mean_circ: 0.934797
Ran for 100550 frames Cell Events: 2864 mean_circ: 0.93481
Ran for 101050 frames Cell Events: 2876 mean_circ: 0.934882
Ran for 101550 frames Cell Events: 2888 mean_circ: 0.934804
Ran for 102550 frames Cell Events: 2930 mean_circ: 0.934834
Ran for 103050 frames Cell Events: 2942 mean_circ: 0.934825
Ran for 103550 frames Cell Events: 2944 mean_circ: 0.934775
Ran for 104550 frames Cell Events: 2968 mean_circ: 0.934842
Ran for 105050 frames Cell Events: 2987 mean_circ: 0.934779
Ran for 105550 frames Cell Events: 2996 mean_circ: 0.934793
Ran for 106050 frames Cell Events: 3013 mean_circ: 0.934784
Ran for 106550 frames Cell Events: 3028 mean_circ: 0.934703
Ran for 107050 frames Cell Events: 3039 mean_circ: 0.934672
Ran for 107550 frames Cell Events: 3048 mean_circ: 0.934709
Ran for 108050 frames Cell Events: 3072 mean_circ: 0.934737
Ran for 108550 frames Cell Events: 3085 mean_circ: 0.934657
Ran for 109050 frames Cell Events: 3100 mean_circ: 0.934619
Ran for 109550 frames Cell Events: 3115 mean_circ: 0.934619
Ran for 110050 frames Cell Events: 3125 mean_circ: 0.934599
Ran for 111050 frames Cell Events: 3156 mean_circ: 0.934635
Ran for 112050 frames Cell Events: 3193 mean_circ: 0.934695
Ran for 114050 frames Cell Events: 3251 mean_circ: 0.934653
Ran for 114550 frames Cell Events: 3265 mean_circ: 0.934608
Ran for 115550 frames Cell Events: 3300 mean_circ: 0.93445
Ran for 116050 frames Cell Events: 3313 mean_circ: 0.934503
Ran for 116550 frames Cell Events: 3335 mean_circ: 0.934476
Ran for 117050 frames Cell Events: 3346 mean_circ: 0.934361
Ran for 117550 frames Cell Events: 3360 mean_circ: 0.934398
Ran for 118050 frames Cell Events: 3381 mean_circ: 0.934452
Ran for 118550 frames Cell Events: 3399 mean_circ: 0.934491
Ran for 119050 frames Cell Events: 3410 mean_circ: 0.934485
Ran for 119550 frames Cell Events: 3456 mean_circ: 0.934436
Ran for 120050 frames Cell Events: 3469 mean_circ: 0.934445
Ran for 120550 frames Cell Events: 3485 mean_circ: 0.934462
Ran for 121050 frames Cell Events: 3497 mean_circ: 0.934534
Ran for 122050 frames Cell Events: 3523 mean_circ: 0.934571
Ran for 122550 frames Cell Events: 3535 mean_circ: 0.934588
Ran for 123050 frames Cell Events: 3553 mean_circ: 0.934643
Ran for 123550 frames Cell Events: 3566 mean_circ: 0.934622
Ran for 124050 frames Cell Events: 3592 mean_circ: 0.934703
Ran for 124550 frames Cell Events: 3611 mean_circ: 0.934674
Ran for 125050 frames Cell Events: 3632 mean_circ: 0.934665
Ran for 125550 frames Cell Events: 3643 mean_circ: 0.934623
Ran for 126050 frames Cell Events: 3660 mean_circ: 0.934626
Ran for 126550 frames Cell Events: 3667 mean_circ: 0.934556
Ran for 127050 frames Cell Events: 3676 mean_circ: 0.934534
Ran for 127550 frames Cell Events: 3685 mean_circ: 0.934527
Ran for 128050 frames Cell Events: 3707 mean_circ: 0.934429
Ran for 128550 frames Cell Events: 3719 mean_circ: 0.934494
Ran for 129050 frames Cell Events: 3734 mean_circ: 0.934502
Ran for 129550 frames Cell Events: 3751 mean_circ: 0.934526
Ran for 130550 frames Cell Events: 3776 mean_circ: 0.934514
Ran for 131050 frames Cell Events: 3794 mean_circ: 0.934471
Ran for 131550 frames Cell Events: 3805 mean_circ: 0.934452
Ran for 132050 frames Cell Events: 3813 mean_circ: 0.934373
Ran for 133050 frames Cell Events: 3857 mean_circ: 0.934323
Ran for 133550 frames Cell Events: 3878 mean_circ: 0.934321
Ran for 134050 frames Cell Events: 3898 mean_circ: 0.934279
Ran for 134550 frames Cell Events: 3911 mean_circ: 0.934353
Ran for 135550 frames Cell Events: 3934 mean_circ: 0.934314
Ran for 136550 frames Cell Events: 3973 mean_circ: 0.934272
Ran for 137050 frames Cell Events: 3984 mean_circ: 0.934265
Ran for 137550 frames Cell Events: 3998 mean_circ: 0.934232
Ran for 138050 frames Cell Events: 4009 mean_circ: 0.934197
Ran for 138550 frames Cell Events: 4019 mean_circ: 0.934167
Ran for 139050 frames Cell Events: 4028 mean_circ: 0.934165
Ran for 139550 frames Cell Events: 4037 mean_circ: 0.934182
Ran for 140550 frames Cell Events: 4057 mean_circ: 0.934192
Ran for 141550 frames Cell Events: 4085 mean_circ: 0.934178
Ran for 142550 frames Cell Events: 4115 mean_circ: 0.934175
Ran for 143050 frames Cell Events: 4137 mean_circ: 0.934178
Ran for 145050 frames Cell Events: 4227 mean_circ: 0.934273
Ran for 146050 frames Cell Events: 4261 mean_circ: 0.934268
Ran for 146550 frames Cell Events: 4274 mean_circ: 0.934328
Ran for 147050 frames Cell Events: 4285 mean_circ: 0.934316
Ran for 147550 frames Cell Events: 4299 mean_circ: 0.934299
Ran for 148050 frames Cell Events: 4316 mean_circ: 0.93434
Ran for 149050 frames Cell Events: 4355 mean_circ: 0.934366
Ran for 149550 frames Cell Events: 4370 mean_circ: 0.934446
Ran for 150550 frames Cell Events: 4420 mean_circ: 0.934361
Ran for 152050 frames Cell Events: 4462 mean_circ: 0.934488
Ran for 153550 frames Cell Events: 4514 mean_circ: 0.934451
Ran for 154550 frames Cell Events: 4539 mean_circ: 0.934467
Ran for 155050 frames Cell Events: 4556 mean_circ: 0.934469
Ran for 156050 frames Cell Events: 4586 mean_circ: 0.934492
Ran for 156550 frames Cell Events: 4601 mean_circ: 0.934511
Ran for 157050 frames Cell Events: 4616 mean_circ: 0.934499
Ran for 158050 frames Cell Events: 4640 mean_circ: 0.934513
Ran for 158550 frames Cell Events: 4645 mean_circ: 0.934476
Ran for 159050 frames Cell Events: 4656 mean_circ: 0.934479
Ran for 159550 frames Cell Events: 4670 mean_circ: 0.934485
Ran for 160050 frames Cell Events: 4681 mean_circ: 0.934498
Ran for 160550 frames Cell Events: 4691 mean_circ: 0.934535
Ran for 161050 frames Cell Events: 4703 mean_circ: 0.934554
Ran for 161550 frames Cell Events: 4722 mean_circ: 0.934515
Ran for 165050 frames Cell Events: 4825 mean_circ: 0.934438
Ran for 165550 frames Cell Events: 4842 mean_circ: 0.934411
Ran for 166050 frames Cell Events: 4858 mean_circ: 0.934469
Ran for 166550 frames Cell Events: 4869 mean_circ: 0.934458
Ran for 168050 frames Cell Events: 4924 mean_circ: 0.9345
Ran for 168550 frames Cell Events: 4934 mean_circ: 0.934525
Ran for 169050 frames Cell Events: 4964 mean_circ: 0.93453
Ran for 169550 frames Cell Events: 4983 mean_circ: 0.934589
Ran for 170050 frames Cell Events: 5000 mean_circ: 0.934642
Ran for 170550 frames Cell Events: 5011 mean_circ: 0.934582
Ran for 171550 frames Cell Events: 5034 mean_circ: 0.934601
Ran for 172050 frames Cell Events: 5052 mean_circ: 0.934573
Ran for 172550 frames Cell Events: 5064 mean_circ: 0.934595
Ran for 173050 frames Cell Events: 5085 mean_circ: 0.934586
Ran for 174050 frames Cell Events: 5104 mean_circ: 0.934559
Ran for 175050 frames Cell Events: 5134 mean_circ: 0.934568
Ran for 175550 frames Cell Events: 5142 mean_circ: 0.934578
Ran for 177050 frames Cell Events: 5192 mean_circ: 0.934554
Ran for 177550 frames Cell Events: 5210 mean_circ: 0.934545
Ran for 178550 frames Cell Events: 5243 mean_circ: 0.934592
Ran for 181550 frames Cell Events: 5328 mean_circ: 0.934609
Ran for 182050 frames Cell Events: 5342 mean_circ: 0.934607
Ran for 182550 frames Cell Events: 5352 mean_circ: 0.934593
Ran for 183550 frames Cell Events: 5400 mean_circ: 0.934581
Ran for 184550 frames Cell Events: 5434 mean_circ: 0.93455
Ran for 185050 frames Cell Events: 5442 mean_circ: 0.934488
Ran for 185550 frames Cell Events: 5454 mean_circ: 0.934504
Ran for 186550 frames Cell Events: 5486 mean_circ: 0.934548
Ran for 187050 frames Cell Events: 5498 mean_circ: 0.934563
Ran for 188550 frames Cell Events: 5546 mean_circ: 0.934533
Ran for 189050 frames Cell Events: 5565 mean_circ: 0.934525
Ran for 189550 frames Cell Events: 5576 mean_circ: 0.934542
Ran for 190050 frames Cell Events: 5592 mean_circ: 0.934523
Ran for 191550 frames Cell Events: 5641 mean_circ: 0.934589
Ran for 192050 frames Cell Events: 5652 mean_circ: 0.93452
Ran for 193550 frames Cell Events: 5691 mean_circ: 0.934509
Ran for 194050 frames Cell Events: 5708 mean_circ: 0.934505
Ran for 194550 frames Cell Events: 5725 mean_circ: 0.934508
Ran for 195550 frames Cell Events: 5767 mean_circ: 0.934514
Ran for 196050 frames Cell Events: 5778 mean_circ: 0.934534
Ran for 197050 frames Cell Events: 5805 mean_circ: 0.934517
Ran for 197550 frames Cell Events: 5818 mean_circ: 0.934523
Ran for 198550 frames Cell Events: 5842 mean_circ: 0.934485
Ran for 199050 frames Cell Events: 5856 mean_circ: 0.934474
Ran for 199550 frames Cell Events: 5871 mean_circ: 0.934448
Ran for 200050 frames Cell Events: 5884 mean_circ: 0.934451
Ran for 200550 frames Cell Events: 5896 mean_circ: 0.934466
Ran for 201050 frames Cell Events: 5907 mean_circ: 0.934473
Ran for 201550 frames Cell Events: 5915 mean_circ: 0.934446
Ran for 203550 frames Cell Events: 5968 mean_circ: 0.934458
Ran for 204050 frames Cell Events: 5981 mean_circ: 0.934485
Ran for 205050 frames Cell Events: 6012 mean_circ: 0.934485
Ran for 206050 frames Cell Events: 6043 mean_circ: 0.934434
Ran for 207050 frames Cell Events: 6081 mean_circ: 0.934422
Ran for 208050 frames Cell Events: 6112 mean_circ: 0.934397
Ran for 208550 frames Cell Events: 6139 mean_circ: 0.934381
Ran for 209550 frames Cell Events: 6164 mean_circ: 0.934326
Ran for 210050 frames Cell Events: 6187 mean_circ: 0.93435
Ran for 211050 frames Cell Events: 6215 mean_circ: 0.934297
Ran for 212050 frames Cell Events: 6243 mean_circ: 0.93429
Ran for 213050 frames Cell Events: 6274 mean_circ: 0.934296
Ran for 215050 frames Cell Events: 6345 mean_circ: 0.934252
Ran for 216550 frames Cell Events: 6388 mean_circ: 0.934291
Ran for 217050 frames Cell Events: 6403 mean_circ: 0.934261
Ran for 217550 frames Cell Events: 6420 mean_circ: 0.934257
Ran for 218050 frames Cell Events: 6438 mean_circ: 0.934246
Ran for 218550 frames Cell Events: 6453 mean_circ: 0.934237
Ran for 219050 frames Cell Events: 6466 mean_circ: 0.93429
Ran for 220550 frames Cell Events: 6507 mean_circ: 0.934268
Ran for 222050 frames Cell Events: 6546 mean_circ: 0.934283
Ran for 222550 frames Cell Events: 6555 mean_circ: 0.934288
Ran for 224550 frames Cell Events: 6613 mean_circ: 0.934258
Ran for 225050 frames Cell Events: 6625 mean_circ: 0.934282
Ran for 225550 frames Cell Events: 6637 mean_circ: 0.934264
Ran for 226050 frames Cell Events: 6652 mean_circ: 0.934235
Ran for 226550 frames Cell Events: 6663 mean_circ: 0.93422
Ran for 227550 frames Cell Events: 6679 mean_circ: 0.934197
Ran for 229550 frames Cell Events: 6742 mean_circ: 0.9341
Ran for 230050 frames Cell Events: 6759 mean_circ: 0.93408
Ran for 231050 frames Cell Events: 6795 mean_circ: 0.934064
Ran for 231550 frames Cell Events: 6809 mean_circ: 0.934048
Ran for 232050 frames Cell Events: 6825 mean_circ: 0.934083
Ran for 233050 frames Cell Events: 6851 mean_circ: 0.934119
Ran for 234050 frames Cell Events: 6878 mean_circ: 0.934111
Ran for 234550 frames Cell Events: 6891 mean_circ: 0.934106
Ran for 235050 frames Cell Events: 6905 mean_circ: 0.93411
Ran for 235550 frames Cell Events: 6923 mean_circ: 0.934089
Ran for 237050 frames Cell Events: 6968 mean_circ: 0.934143
Ran for 237550 frames Cell Events: 6979 mean_circ: 0.934142
Ran for 238050 frames Cell Events: 6987 mean_circ: 0.934136
Ran for 238550 frames Cell Events: 7008 mean_circ: 0.934035
Ran for 239050 frames Cell Events: 7022 mean_circ: 0.934043
Ran for 239550 frames Cell Events: 7029 mean_circ: 0.934039
Ran for 240050 frames Cell Events: 7038 mean_circ: 0.934067
Ran for 243050 frames Cell Events: 7182 mean_circ: 0.934153
Ran for 243550 frames Cell Events: 7200 mean_circ: 0.934161
Ran for 244050 frames Cell Events: 7225 mean_circ: 0.934167
Ran for 245050 frames Cell Events: 7281 mean_circ: 0.934153
Ran for 246050 frames Cell Events: 7337 mean_circ: 0.934165
Ran for 246550 frames Cell Events: 7350 mean_circ: 0.934191
Ran for 247050 frames Cell Events: 7365 mean_circ: 0.934199
Ran for 247550 frames Cell Events: 7379 mean_circ: 0.934193
Ran for 248050 frames Cell Events: 7394 mean_circ: 0.934179
Ran for 249550 frames Cell Events: 7436 mean_circ: 0.934114
Ran for 250050 frames Cell Events: 7441 mean_circ: 0.934111
Ran for 250550 frames Cell Events: 7454 mean_circ: 0.934095
Ran for 251050 frames Cell Events: 7467 mean_circ: 0.934094
Ran for 252050 frames Cell Events: 7496 mean_circ: 0.934127
Ran for 252550 frames Cell Events: 7520 mean_circ: 0.934148
Ran for 253050 frames Cell Events: 7536 mean_circ: 0.934143
Ran for 254050 frames Cell Events: 7588 mean_circ: 0.934177
Ran for 254550 frames Cell Events: 7599 mean_circ: 0.934165
Ran for 255050 frames Cell Events: 7610 mean_circ: 0.934168
Ran for 256050 frames Cell Events: 7645 mean_circ: 0.934116
Ran for 257550 frames Cell Events: 7695 mean_circ: 0.934087
Ran for 259550 frames Cell Events: 7756 mean_circ: 0.93401
Ran for 261550 frames Cell Events: 7833 mean_circ: 0.933907
Ran for 263550 frames Cell Events: 7876 mean_circ: 0.933946
Ran for 265550 frames Cell Events: 7930 mean_circ: 0.933954
Ran for 266050 frames Cell Events: 7948 mean_circ: 0.933965
Ran for 267550 frames Cell Events: 8003 mean_circ: 0.933913
Ran for 268050 frames Cell Events: 8034 mean_circ: 0.933964



End this measurment..



Stopping time: 4.0392e+008
****************************************
Measurement finished

Mean Circularitiy: 0.933848
Mean Area: 674.701
Mean FL-1 intensity: 2595.92
Mean FL-2 intensity: 3361.83
Mean FL-3 intensity: 0

frames processed: 266006
Total Time: 134105ms
Number of Runs (new bg img) 0
Frames collected: 268065
Skipped frames: 14801
Rescued Frames: 1918975
Samples DAQ: 67016485
Samples written: 134032252
Samples per Channel: 67016126
FPS: 1998.92

Samples per second: 499728
Expected samples/s: 500000

Events: 8035
Events per second: 59.9157
jumped back (PMT): 5468
#Frames read, frequency
# 0 frames :380473
# 1 frames :134703
# 2 frames :5459
# 3 frames :2176
# 4 frames :1251
# 5 frames :647
# 6 frames :916
# 7 frames :1879
# 8 frames :1072
# 9 frames :800
# 10 frames :1370
# 11 frames :729
# 12 frames :633
# 13 frames :350
# 14 frames :274
# 15 frames :234
# 16 frames :244
# 17 frames :193
# 18 frames :138
# 19 frames :127
# 20 frames :87
# 21 frames :102
# 22 frames :86
# 23 frames :71
# 24 frames :60
# 25 frames :47
# 26 frames :33
# 27 frames :23
# 28 frames :23
# 29 frames :20
# 30 frames :27
# 31 frames :10
# 32 frames :16
# 33 frames :7
# 34 frames :10
# 35 frames :14
# 36 frames :8
# 37 frames :7
# 38 frames :5
# 39 frames :4
# 40 frames :6
# 41 frames :5
# 42 frames :2
# 43 frames :5
# 44 frames :3
# 45 frames :4
# 46 frames :2
# 48 frames :3
# 49 frames :3
# 50 frames :3
# 51 frames :1
# 52 frames :1
# 53 frames :1
# 54 frames :3
# 55 frames :1
# 58 frames :1
# 65 frames :2
# 69 frames :1
# 76 frames :1
# 96 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0081
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0