Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_19.09.35 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "ef8d9328b56f8523f2e2e8084403c469",
"name": "M1_20170619_1346_dsRNA_V7_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "17:10:01"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 10002 (max 10003) in
'10002'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.993
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 4.70146e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1550 frames Cell Events: 79 mean_circ: 0.92816
Ran for 2050 frames Cell Events: 102 mean_circ: 0.929275
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Ran for 13050 frames Cell Events: 569 mean_circ: 0.937107
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Ran for 100550 frames Cell Events: 4456 mean_circ: 0.934976
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Ran for 102550 frames Cell Events: 4530 mean_circ: 0.935033
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Ran for 103550 frames Cell Events: 4563 mean_circ: 0.935093
Ran for 104050 frames Cell Events: 4587 mean_circ: 0.93508
Ran for 106550 frames Cell Events: 4722 mean_circ: 0.93514
Ran for 107050 frames Cell Events: 4750 mean_circ: 0.935161
Ran for 107550 frames Cell Events: 4766 mean_circ: 0.935214
Ran for 108050 frames Cell Events: 4783 mean_circ: 0.935251
Ran for 108550 frames Cell Events: 4798 mean_circ: 0.935287
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Ran for 121550 frames Cell Events: 5351 mean_circ: 0.935246
Ran for 123050 frames Cell Events: 5428 mean_circ: 0.935271
Ran for 123550 frames Cell Events: 5445 mean_circ: 0.935204
Ran for 124050 frames Cell Events: 5462 mean_circ: 0.935192
Ran for 124550 frames Cell Events: 5483 mean_circ: 0.935254
Ran for 125050 frames Cell Events: 5497 mean_circ: 0.935279
Ran for 125550 frames Cell Events: 5520 mean_circ: 0.935329
Ran for 126050 frames Cell Events: 5542 mean_circ: 0.935311
Ran for 127550 frames Cell Events: 5600 mean_circ: 0.935324
Ran for 129050 frames Cell Events: 5678 mean_circ: 0.935301
Ran for 129550 frames Cell Events: 5705 mean_circ: 0.935252
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Ran for 143050 frames Cell Events: 6310 mean_circ: 0.935331
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Ran for 211050 frames Cell Events: 9513 mean_circ: 0.93506
Ran for 211550 frames Cell Events: 9532 mean_circ: 0.93505
Ran for 212050 frames Cell Events: 9552 mean_circ: 0.935055
Ran for 212550 frames Cell Events: 9573 mean_circ: 0.935079
Ran for 213050 frames Cell Events: 9587 mean_circ: 0.935102
Ran for 214050 frames Cell Events: 9629 mean_circ: 0.935132
Ran for 216550 frames Cell Events: 9709 mean_circ: 0.935115
Ran for 217050 frames Cell Events: 9725 mean_circ: 0.935088
Ran for 217550 frames Cell Events: 9746 mean_circ: 0.935037
Ran for 219050 frames Cell Events: 9814 mean_circ: 0.935093
Ran for 219550 frames Cell Events: 9837 mean_circ: 0.935131
Ran for 220050 frames Cell Events: 9849 mean_circ: 0.93513
Ran for 220550 frames Cell Events: 9878 mean_circ: 0.935158
Ran for 221050 frames Cell Events: 9887 mean_circ: 0.935165
Ran for 221550 frames Cell Events: 9913 mean_circ: 0.93516
Ran for 222550 frames Cell Events: 9943 mean_circ: 0.93515
Ran for 223050 frames Cell Events: 9964 mean_circ: 0.935138
Ran for 223550 frames Cell Events: 9981 mean_circ: 0.935156
Ran for 224050 frames Cell Events: 9989 mean_circ: 0.935149



End this measurment.
because stop condition (time/nr of cells) was met

Stopping time: 4.70259e+008
****************************************
Measurement finished

Mean Circularitiy: 0.93516
Mean Area: 588.005
Mean FL-1 intensity: 3266.98
Mean FL-2 intensity: 4355.83
Mean FL-3 intensity: 0

frames processed: 224148
Total Time: 112280ms
Number of Runs (new bg img) 0
Frames collected: 224406
Skipped frames: 2868
Rescued Frames: 556078
Samples DAQ: 56101832
Samples written: 112203054
Samples per Channel: 56101527
FPS: 1998.62

Samples per second: 499655
Expected samples/s: 500000

Events: 10003
Events per second: 89.0894
jumped back (PMT): 25
#Frames read, frequency
# 0 frames :328529
# 1 frames :101029
# 2 frames :5523
# 3 frames :1831
# 4 frames :1048
# 5 frames :448
# 6 frames :2009
# 7 frames :2254
# 8 frames :1130
# 9 frames :757
# 10 frames :1089
# 11 frames :776
# 12 frames :592
# 13 frames :312
# 14 frames :218
# 15 frames :248
# 16 frames :197
# 17 frames :199
# 18 frames :116
# 19 frames :60
# 20 frames :50
# 21 frames :61
# 22 frames :43
# 23 frames :43
# 24 frames :34
# 25 frames :19
# 26 frames :16
# 27 frames :9
# 28 frames :8
# 29 frames :16
# 30 frames :8
# 31 frames :4
# 32 frames :13
# 33 frames :8
# 34 frames :6
# 35 frames :7
# 36 frames :3
# 37 frames :1
# 38 frames :2
# 39 frames :1
# 40 frames :4
# 41 frames :5
# 44 frames :1
# 45 frames :3
# 47 frames :2
# 48 frames :1
# 49 frames :1
# 54 frames :1
# 57 frames :2
# 90 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.993
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0