Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_19.10.09 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "d6552692f5f2de944b3db9c5308ee8e8",
"name": "M1_20170619_1356_dsRNA_U5_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "17:10:35"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 10001 (max 10002) in
'10001'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.9698
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 4.70713e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 22 mean_circ: 0.946488
Ran for 1050 frames Cell Events: 45 mean_circ: 0.945417
Ran for 1550 frames Cell Events: 65 mean_circ: 0.947296
Ran for 2050 frames Cell Events: 86 mean_circ: 0.949997
Ran for 2550 frames Cell Events: 108 mean_circ: 0.946629
Ran for 3050 frames Cell Events: 137 mean_circ: 0.94597
Ran for 3550 frames Cell Events: 168 mean_circ: 0.943147
Ran for 4050 frames Cell Events: 194 mean_circ: 0.941967
Ran for 4550 frames Cell Events: 221 mean_circ: 0.943031
Ran for 5550 frames Cell Events: 274 mean_circ: 0.942249
Ran for 6050 frames Cell Events: 294 mean_circ: 0.942447
Ran for 7050 frames Cell Events: 354 mean_circ: 0.944007
Ran for 9050 frames Cell Events: 464 mean_circ: 0.943849
Ran for 9550 frames Cell Events: 484 mean_circ: 0.942899
Ran for 11050 frames Cell Events: 559 mean_circ: 0.942626
Ran for 12050 frames Cell Events: 615 mean_circ: 0.943299
Ran for 12550 frames Cell Events: 637 mean_circ: 0.943488
Ran for 14550 frames Cell Events: 745 mean_circ: 0.943287
Ran for 15050 frames Cell Events: 773 mean_circ: 0.943466
Ran for 17050 frames Cell Events: 886 mean_circ: 0.943484
Ran for 18050 frames Cell Events: 928 mean_circ: 0.943533
Ran for 19050 frames Cell Events: 1010 mean_circ: 0.943567
Ran for 19550 frames Cell Events: 1049 mean_circ: 0.943583
Ran for 20550 frames Cell Events: 1089 mean_circ: 0.943356
Ran for 22050 frames Cell Events: 1148 mean_circ: 0.943597
Ran for 23050 frames Cell Events: 1197 mean_circ: 0.943424
Ran for 24550 frames Cell Events: 1287 mean_circ: 0.943019
Ran for 25050 frames Cell Events: 1321 mean_circ: 0.943029
Ran for 25550 frames Cell Events: 1341 mean_circ: 0.943248
Ran for 26550 frames Cell Events: 1397 mean_circ: 0.943137
Ran for 28050 frames Cell Events: 1471 mean_circ: 0.943502
Ran for 28550 frames Cell Events: 1496 mean_circ: 0.943619
Ran for 29050 frames Cell Events: 1529 mean_circ: 0.943576
Ran for 29550 frames Cell Events: 1544 mean_circ: 0.943563
Ran for 30550 frames Cell Events: 1580 mean_circ: 0.943772
Ran for 31050 frames Cell Events: 1597 mean_circ: 0.943757
Ran for 31550 frames Cell Events: 1623 mean_circ: 0.9436
Ran for 32550 frames Cell Events: 1668 mean_circ: 0.943667
Ran for 33050 frames Cell Events: 1695 mean_circ: 0.943538
Ran for 34050 frames Cell Events: 1761 mean_circ: 0.943463
Ran for 35050 frames Cell Events: 1822 mean_circ: 0.943585
Ran for 36050 frames Cell Events: 1889 mean_circ: 0.943268
Ran for 37550 frames Cell Events: 1976 mean_circ: 0.943378
Ran for 38050 frames Cell Events: 1998 mean_circ: 0.943322
Ran for 38550 frames Cell Events: 2027 mean_circ: 0.943403
Ran for 40050 frames Cell Events: 2131 mean_circ: 0.943447
Ran for 41050 frames Cell Events: 2186 mean_circ: 0.94352
Ran for 42050 frames Cell Events: 2241 mean_circ: 0.943491
Ran for 43050 frames Cell Events: 2303 mean_circ: 0.943488
Ran for 44050 frames Cell Events: 2364 mean_circ: 0.943438
Ran for 45050 frames Cell Events: 2433 mean_circ: 0.943344
Ran for 45550 frames Cell Events: 2457 mean_circ: 0.943423
Ran for 46050 frames Cell Events: 2482 mean_circ: 0.943322
Ran for 46550 frames Cell Events: 2522 mean_circ: 0.943285
Ran for 47050 frames Cell Events: 2549 mean_circ: 0.943362
Ran for 48050 frames Cell Events: 2611 mean_circ: 0.943279
Ran for 49050 frames Cell Events: 2669 mean_circ: 0.943198
Ran for 49550 frames Cell Events: 2698 mean_circ: 0.943291
Ran for 50050 frames Cell Events: 2722 mean_circ: 0.943316
Ran for 50550 frames Cell Events: 2755 mean_circ: 0.94338
Ran for 51550 frames Cell Events: 2824 mean_circ: 0.943331
Ran for 54050 frames Cell Events: 2945 mean_circ: 0.943369
Ran for 54550 frames Cell Events: 2969 mean_circ: 0.943314
Ran for 55550 frames Cell Events: 3010 mean_circ: 0.943378
Ran for 57550 frames Cell Events: 3120 mean_circ: 0.943108
Ran for 60050 frames Cell Events: 3284 mean_circ: 0.942788
Ran for 60550 frames Cell Events: 3309 mean_circ: 0.942863
Ran for 62050 frames Cell Events: 3387 mean_circ: 0.942835
Ran for 62550 frames Cell Events: 3410 mean_circ: 0.942851
Ran for 63050 frames Cell Events: 3436 mean_circ: 0.942849
Ran for 63550 frames Cell Events: 3464 mean_circ: 0.942795
Ran for 64050 frames Cell Events: 3501 mean_circ: 0.942795
Ran for 66050 frames Cell Events: 3621 mean_circ: 0.942615
Ran for 67550 frames Cell Events: 3710 mean_circ: 0.942584
Ran for 68550 frames Cell Events: 3767 mean_circ: 0.942624
Ran for 70550 frames Cell Events: 3876 mean_circ: 0.942683
Ran for 71550 frames Cell Events: 3930 mean_circ: 0.942692
Ran for 72050 frames Cell Events: 3960 mean_circ: 0.942755
Ran for 72550 frames Cell Events: 3997 mean_circ: 0.942787
Ran for 73550 frames Cell Events: 4048 mean_circ: 0.942796
Ran for 78050 frames Cell Events: 4323 mean_circ: 0.942701
Ran for 80050 frames Cell Events: 4438 mean_circ: 0.942557
Ran for 81050 frames Cell Events: 4504 mean_circ: 0.942541
Ran for 81550 frames Cell Events: 4536 mean_circ: 0.942541
Ran for 83050 frames Cell Events: 4604 mean_circ: 0.942435
Ran for 84050 frames Cell Events: 4646 mean_circ: 0.942455
Ran for 84550 frames Cell Events: 4668 mean_circ: 0.942445
Ran for 85050 frames Cell Events: 4693 mean_circ: 0.942471
Ran for 86550 frames Cell Events: 4768 mean_circ: 0.942535
Ran for 87050 frames Cell Events: 4789 mean_circ: 0.942521
Ran for 89050 frames Cell Events: 4915 mean_circ: 0.942527
Ran for 89550 frames Cell Events: 4950 mean_circ: 0.942501
Ran for 91050 frames Cell Events: 5036 mean_circ: 0.942567
Ran for 92050 frames Cell Events: 5092 mean_circ: 0.942581
Ran for 92550 frames Cell Events: 5119 mean_circ: 0.94262
Ran for 95550 frames Cell Events: 5287 mean_circ: 0.942663
Ran for 96550 frames Cell Events: 5347 mean_circ: 0.942617
Ran for 98050 frames Cell Events: 5428 mean_circ: 0.942581
Ran for 98550 frames Cell Events: 5455 mean_circ: 0.942603
Ran for 99050 frames Cell Events: 5494 mean_circ: 0.942603
Ran for 100550 frames Cell Events: 5592 mean_circ: 0.942555
Ran for 101550 frames Cell Events: 5660 mean_circ: 0.942569
Ran for 102550 frames Cell Events: 5721 mean_circ: 0.942571
Ran for 103050 frames Cell Events: 5742 mean_circ: 0.94258
Ran for 103550 frames Cell Events: 5774 mean_circ: 0.942541
Ran for 104050 frames Cell Events: 5794 mean_circ: 0.942527
Ran for 105550 frames Cell Events: 5870 mean_circ: 0.942523
Ran for 108550 frames Cell Events: 6024 mean_circ: 0.942302
Ran for 110050 frames Cell Events: 6097 mean_circ: 0.942343
Ran for 110550 frames Cell Events: 6119 mean_circ: 0.942354
Ran for 111050 frames Cell Events: 6136 mean_circ: 0.942366
Ran for 114050 frames Cell Events: 6287 mean_circ: 0.942404
Ran for 116050 frames Cell Events: 6406 mean_circ: 0.94243
Ran for 117050 frames Cell Events: 6460 mean_circ: 0.942422
Ran for 118050 frames Cell Events: 6515 mean_circ: 0.942463
Ran for 118550 frames Cell Events: 6541 mean_circ: 0.942448
Ran for 120050 frames Cell Events: 6614 mean_circ: 0.942452
Ran for 121550 frames Cell Events: 6717 mean_circ: 0.942429
Ran for 123050 frames Cell Events: 6777 mean_circ: 0.942423
Ran for 123550 frames Cell Events: 6806 mean_circ: 0.94239
Ran for 124050 frames Cell Events: 6835 mean_circ: 0.942386
Ran for 125050 frames Cell Events: 6894 mean_circ: 0.942356
Ran for 125550 frames Cell Events: 6924 mean_circ: 0.942436
Ran for 126050 frames Cell Events: 6951 mean_circ: 0.942418
Ran for 126550 frames Cell Events: 6972 mean_circ: 0.942449
Ran for 127050 frames Cell Events: 7004 mean_circ: 0.942446
Ran for 129050 frames Cell Events: 7144 mean_circ: 0.942548
Ran for 132550 frames Cell Events: 7373 mean_circ: 0.942424
Ran for 133050 frames Cell Events: 7406 mean_circ: 0.942367
Ran for 133550 frames Cell Events: 7428 mean_circ: 0.942375
Ran for 134550 frames Cell Events: 7476 mean_circ: 0.942371
Ran for 136050 frames Cell Events: 7567 mean_circ: 0.942291
Ran for 136550 frames Cell Events: 7600 mean_circ: 0.942315
Ran for 137050 frames Cell Events: 7617 mean_circ: 0.942314
Ran for 137550 frames Cell Events: 7639 mean_circ: 0.942304
Ran for 139050 frames Cell Events: 7719 mean_circ: 0.94239
Ran for 140050 frames Cell Events: 7759 mean_circ: 0.942366
Ran for 141050 frames Cell Events: 7822 mean_circ: 0.942401
Ran for 143050 frames Cell Events: 7979 mean_circ: 0.942404
Ran for 145050 frames Cell Events: 8148 mean_circ: 0.942203
Ran for 146550 frames Cell Events: 8241 mean_circ: 0.942265
Ran for 147050 frames Cell Events: 8273 mean_circ: 0.942301
Ran for 148050 frames Cell Events: 8322 mean_circ: 0.942351
Ran for 149550 frames Cell Events: 8420 mean_circ: 0.94232
Ran for 150550 frames Cell Events: 8486 mean_circ: 0.942325
Ran for 151050 frames Cell Events: 8518 mean_circ: 0.942333
Ran for 151550 frames Cell Events: 8550 mean_circ: 0.942354
Ran for 155050 frames Cell Events: 8755 mean_circ: 0.942396
Ran for 155550 frames Cell Events: 8783 mean_circ: 0.942422
Ran for 157050 frames Cell Events: 8859 mean_circ: 0.942348
Ran for 158050 frames Cell Events: 8911 mean_circ: 0.942373
Ran for 158550 frames Cell Events: 8927 mean_circ: 0.942388
Ran for 159550 frames Cell Events: 8967 mean_circ: 0.942342
Ran for 160550 frames Cell Events: 9018 mean_circ: 0.942358
Ran for 161050 frames Cell Events: 9045 mean_circ: 0.942358
Ran for 162050 frames Cell Events: 9095 mean_circ: 0.942335
Ran for 164050 frames Cell Events: 9219 mean_circ: 0.942429
Ran for 165050 frames Cell Events: 9284 mean_circ: 0.942456
Ran for 165550 frames Cell Events: 9303 mean_circ: 0.942428
Ran for 167550 frames Cell Events: 9426 mean_circ: 0.94233
Ran for 169050 frames Cell Events: 9518 mean_circ: 0.942325
Ran for 169550 frames Cell Events: 9538 mean_circ: 0.942328
Ran for 170050 frames Cell Events: 9556 mean_circ: 0.942293
Ran for 171550 frames Cell Events: 9656 mean_circ: 0.942293
Ran for 173550 frames Cell Events: 9777 mean_circ: 0.942345
Ran for 174050 frames Cell Events: 9806 mean_circ: 0.942337
Ran for 177050 frames Cell Events: 9974 mean_circ: 0.942313



End this measurment.
because stop condition (time/nr of cells) was met

Stopping time: 4.70802e+008
****************************************
Measurement finished

Mean Circularitiy: 0.942203
Mean Area: 630.362
Mean FL-1 intensity: 3806.07
Mean FL-2 intensity: 3457.37
Mean FL-3 intensity: 0

frames processed: 176468
Total Time: 88848.5ms
Number of Runs (new bg img) 0
Frames collected: 177537
Skipped frames: 3937
Rescued Frames: 655761
Samples DAQ: 44389229
Samples written: 88768208
Samples per Channel: 44384104
FPS: 1998.2

Samples per second: 499548
Expected samples/s: 500000

Events: 10003
Events per second: 112.585
jumped back (PMT): 4580
#Frames read, frequency
# 0 frames :278119
# 1 frames :60651
# 2 frames :4319
# 3 frames :1392
# 4 frames :867
# 5 frames :409
# 6 frames :1877
# 7 frames :1558
# 8 frames :1014
# 9 frames :636
# 10 frames :914
# 11 frames :546
# 12 frames :436
# 13 frames :248
# 14 frames :255
# 15 frames :254
# 16 frames :260
# 17 frames :197
# 18 frames :130
# 19 frames :104
# 20 frames :69
# 21 frames :90
# 22 frames :81
# 23 frames :93
# 24 frames :59
# 25 frames :38
# 26 frames :45
# 27 frames :34
# 28 frames :22
# 29 frames :33
# 30 frames :26
# 31 frames :13
# 32 frames :19
# 33 frames :22
# 34 frames :10
# 35 frames :8
# 36 frames :7
# 37 frames :11
# 38 frames :3
# 39 frames :3
# 40 frames :4
# 41 frames :5
# 42 frames :2
# 43 frames :2
# 44 frames :2
# 45 frames :3
# 46 frames :3
# 47 frames :3
# 48 frames :4
# 49 frames :2
# 50 frames :1
# 51 frames :3
# 52 frames :1
# 54 frames :4
# 57 frames :3
# 58 frames :2
# 60 frames :1
# 65 frames :2
# 67 frames :1
# 69 frames :1
# 92 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.9698
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0