Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_19.10.42 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "b697aafb05849824fae67da10cfc39f3",
"name": "M1_20170619_1358_dsRNA_U5_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "17:11:03"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8271 (max 8272) in '8271'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.9755
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 4.70879e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 2050 frames Cell Events: 122 mean_circ: 0.935745
Ran for 3550 frames Cell Events: 218 mean_circ: 0.929595
Ran for 4050 frames Cell Events: 243 mean_circ: 0.929592
Ran for 4550 frames Cell Events: 269 mean_circ: 0.929862
Ran for 5050 frames Cell Events: 290 mean_circ: 0.929208
Ran for 5550 frames Cell Events: 309 mean_circ: 0.93019
Ran for 6050 frames Cell Events: 332 mean_circ: 0.930134
Ran for 6550 frames Cell Events: 357 mean_circ: 0.930056
Ran for 7050 frames Cell Events: 383 mean_circ: 0.93026
Ran for 7550 frames Cell Events: 416 mean_circ: 0.930662
Ran for 8050 frames Cell Events: 441 mean_circ: 0.930383
Ran for 9050 frames Cell Events: 497 mean_circ: 0.930602
Ran for 9550 frames Cell Events: 523 mean_circ: 0.930667
Ran for 10050 frames Cell Events: 546 mean_circ: 0.930619
Ran for 11550 frames Cell Events: 669 mean_circ: 0.932052
Ran for 12050 frames Cell Events: 699 mean_circ: 0.932545
Ran for 13050 frames Cell Events: 751 mean_circ: 0.931146
Ran for 14550 frames Cell Events: 839 mean_circ: 0.932025
Ran for 15550 frames Cell Events: 903 mean_circ: 0.931231
Ran for 16550 frames Cell Events: 956 mean_circ: 0.931029
Ran for 20050 frames Cell Events: 1212 mean_circ: 0.930078
Ran for 21550 frames Cell Events: 1309 mean_circ: 0.930043
Ran for 22050 frames Cell Events: 1333 mean_circ: 0.930193
Ran for 25550 frames Cell Events: 1543 mean_circ: 0.929958
Ran for 27050 frames Cell Events: 1628 mean_circ: 0.930137
Ran for 27550 frames Cell Events: 1661 mean_circ: 0.93033
Ran for 29550 frames Cell Events: 1789 mean_circ: 0.930301
Ran for 30050 frames Cell Events: 1814 mean_circ: 0.930227
Ran for 32050 frames Cell Events: 1929 mean_circ: 0.929707
Ran for 32550 frames Cell Events: 1950 mean_circ: 0.929652
Ran for 33050 frames Cell Events: 1979 mean_circ: 0.929718
Ran for 35050 frames Cell Events: 2081 mean_circ: 0.930023
Ran for 35550 frames Cell Events: 2107 mean_circ: 0.929961
Ran for 39050 frames Cell Events: 2366 mean_circ: 0.930057
Ran for 40050 frames Cell Events: 2434 mean_circ: 0.930088
Ran for 41550 frames Cell Events: 2516 mean_circ: 0.930015
Ran for 43550 frames Cell Events: 2643 mean_circ: 0.930039
Ran for 44050 frames Cell Events: 2661 mean_circ: 0.930224
Ran for 46050 frames Cell Events: 2763 mean_circ: 0.930154
Ran for 47550 frames Cell Events: 2848 mean_circ: 0.930181
Ran for 48550 frames Cell Events: 2912 mean_circ: 0.929841
Ran for 50550 frames Cell Events: 3012 mean_circ: 0.929741
Ran for 51050 frames Cell Events: 3036 mean_circ: 0.929788
Ran for 52550 frames Cell Events: 3141 mean_circ: 0.929798
Ran for 53550 frames Cell Events: 3216 mean_circ: 0.929607
Ran for 54050 frames Cell Events: 3252 mean_circ: 0.92947
Ran for 55550 frames Cell Events: 3340 mean_circ: 0.929735
Ran for 57050 frames Cell Events: 3461 mean_circ: 0.929783
Ran for 57550 frames Cell Events: 3490 mean_circ: 0.929875
Ran for 58050 frames Cell Events: 3523 mean_circ: 0.929902
Ran for 59050 frames Cell Events: 3587 mean_circ: 0.929741
Ran for 60050 frames Cell Events: 3669 mean_circ: 0.929806
Ran for 61050 frames Cell Events: 3747 mean_circ: 0.929814
Ran for 62550 frames Cell Events: 3851 mean_circ: 0.929888
Ran for 63550 frames Cell Events: 3904 mean_circ: 0.92985
Ran for 66550 frames Cell Events: 4111 mean_circ: 0.92978
Ran for 67550 frames Cell Events: 4162 mean_circ: 0.929645
Ran for 72550 frames Cell Events: 4500 mean_circ: 0.929674
Ran for 73550 frames Cell Events: 4550 mean_circ: 0.929632
Ran for 74050 frames Cell Events: 4568 mean_circ: 0.929652
Ran for 74550 frames Cell Events: 4602 mean_circ: 0.929681
Ran for 76050 frames Cell Events: 4685 mean_circ: 0.929705
Ran for 77050 frames Cell Events: 4764 mean_circ: 0.929597
Ran for 77550 frames Cell Events: 4781 mean_circ: 0.929591
Ran for 78050 frames Cell Events: 4806 mean_circ: 0.929572
Ran for 78550 frames Cell Events: 4841 mean_circ: 0.929597
Ran for 79050 frames Cell Events: 4865 mean_circ: 0.929517
Ran for 79550 frames Cell Events: 4897 mean_circ: 0.929506
Ran for 80550 frames Cell Events: 4942 mean_circ: 0.929544
Ran for 81550 frames Cell Events: 4995 mean_circ: 0.929586
Ran for 82050 frames Cell Events: 5025 mean_circ: 0.929586
Ran for 84050 frames Cell Events: 5175 mean_circ: 0.929585
Ran for 85050 frames Cell Events: 5226 mean_circ: 0.929526
Ran for 85550 frames Cell Events: 5257 mean_circ: 0.929555
Ran for 86050 frames Cell Events: 5289 mean_circ: 0.929534
Ran for 86550 frames Cell Events: 5321 mean_circ: 0.929568
Ran for 89050 frames Cell Events: 5474 mean_circ: 0.929582
Ran for 91050 frames Cell Events: 5593 mean_circ: 0.929525
Ran for 94050 frames Cell Events: 5785 mean_circ: 0.929437
Ran for 95550 frames Cell Events: 5884 mean_circ: 0.929291
Ran for 99050 frames Cell Events: 6114 mean_circ: 0.929215
Ran for 99550 frames Cell Events: 6143 mean_circ: 0.929143
Ran for 102550 frames Cell Events: 6294 mean_circ: 0.92917
Ran for 104050 frames Cell Events: 6409 mean_circ: 0.929218
Ran for 104550 frames Cell Events: 6440 mean_circ: 0.92918
Ran for 106550 frames Cell Events: 6541 mean_circ: 0.929262
Ran for 110050 frames Cell Events: 6727 mean_circ: 0.929367
Ran for 110550 frames Cell Events: 6751 mean_circ: 0.929287
Ran for 111050 frames Cell Events: 6792 mean_circ: 0.929167
Ran for 111550 frames Cell Events: 6824 mean_circ: 0.929143
Ran for 115050 frames Cell Events: 7034 mean_circ: 0.92915
Ran for 115550 frames Cell Events: 7065 mean_circ: 0.929154
Ran for 116050 frames Cell Events: 7090 mean_circ: 0.929098
Ran for 117550 frames Cell Events: 7172 mean_circ: 0.929095
Ran for 120050 frames Cell Events: 7338 mean_circ: 0.928887
Ran for 120550 frames Cell Events: 7352 mean_circ: 0.928895
Ran for 121050 frames Cell Events: 7380 mean_circ: 0.92892
Ran for 121550 frames Cell Events: 7414 mean_circ: 0.928883
Ran for 122550 frames Cell Events: 7475 mean_circ: 0.928925
Ran for 125050 frames Cell Events: 7640 mean_circ: 0.928939
Ran for 125550 frames Cell Events: 7660 mean_circ: 0.928925
Ran for 126050 frames Cell Events: 7686 mean_circ: 0.928901
Ran for 126550 frames Cell Events: 7718 mean_circ: 0.928895
Ran for 127050 frames Cell Events: 7745 mean_circ: 0.928877
Ran for 128050 frames Cell Events: 7813 mean_circ: 0.928861
Ran for 128550 frames Cell Events: 7846 mean_circ: 0.928823
Ran for 129550 frames Cell Events: 7923 mean_circ: 0.928804
Ran for 130550 frames Cell Events: 7997 mean_circ: 0.92871
Ran for 132550 frames Cell Events: 8123 mean_circ: 0.928724
Ran for 134550 frames Cell Events: 8217 mean_circ: 0.928711



End this measurment..



Stopping time: 4.70947e+008
****************************************
Measurement finished

Mean Circularitiy: 0.928724
Mean Area: 632.292
Mean FL-1 intensity: 3469.38
Mean FL-2 intensity: 3733.84
Mean FL-3 intensity: 0

frames processed: 135127
Total Time: 67871.8ms
Number of Runs (new bg img) 0
Frames collected: 135653
Skipped frames: 4464
Rescued Frames: 734564
Samples DAQ: 33913373
Samples written: 67826338
Samples per Channel: 33913169
FPS: 1998.66

Samples per second: 499665
Expected samples/s: 500000

Events: 8272
Events per second: 121.877
jumped back (PMT): 17
#Frames read, frequency
# 0 frames :214944
# 1 frames :44338
# 2 frames :2710
# 3 frames :1099
# 4 frames :591
# 5 frames :353
# 6 frames :1770
# 7 frames :946
# 8 frames :819
# 9 frames :542
# 10 frames :621
# 11 frames :491
# 12 frames :287
# 13 frames :212
# 14 frames :210
# 15 frames :200
# 16 frames :173
# 17 frames :151
# 18 frames :122
# 19 frames :87
# 20 frames :77
# 21 frames :61
# 22 frames :56
# 23 frames :54
# 24 frames :58
# 25 frames :27
# 26 frames :23
# 27 frames :26
# 28 frames :20
# 29 frames :18
# 30 frames :21
# 31 frames :23
# 32 frames :17
# 33 frames :18
# 34 frames :8
# 35 frames :15
# 36 frames :7
# 37 frames :13
# 38 frames :8
# 39 frames :6
# 40 frames :5
# 41 frames :6
# 42 frames :2
# 43 frames :3
# 44 frames :7
# 45 frames :7
# 46 frames :6
# 47 frames :2
# 48 frames :3
# 49 frames :3
# 50 frames :2
# 51 frames :1
# 52 frames :2
# 53 frames :1
# 54 frames :1
# 57 frames :1
# 61 frames :1
# 67 frames :1
# 79 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.9755
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0