Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_19.11.39 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "447ebabe5c09f7776c75629ebccf6b3f",
"name": "M1_20170619_1412_dsRNA_V4_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "17:11:59"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8058 (max 8059) in '8058'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1235
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 4.7171e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 32 mean_circ: 0.93333
Ran for 1050 frames Cell Events: 49 mean_circ: 0.939814
Ran for 2050 frames Cell Events: 111 mean_circ: 0.942835
Ran for 2550 frames Cell Events: 146 mean_circ: 0.944417
Ran for 3550 frames Cell Events: 205 mean_circ: 0.939869
Ran for 4050 frames Cell Events: 229 mean_circ: 0.939983
Ran for 4550 frames Cell Events: 249 mean_circ: 0.939227
Ran for 5050 frames Cell Events: 282 mean_circ: 0.939327
Ran for 6050 frames Cell Events: 349 mean_circ: 0.940009
Ran for 6550 frames Cell Events: 383 mean_circ: 0.938936
Ran for 7050 frames Cell Events: 410 mean_circ: 0.938521
Ran for 8050 frames Cell Events: 484 mean_circ: 0.938573
Ran for 9550 frames Cell Events: 575 mean_circ: 0.938405
Ran for 11050 frames Cell Events: 654 mean_circ: 0.939136
Ran for 13050 frames Cell Events: 755 mean_circ: 0.939236
Ran for 13550 frames Cell Events: 777 mean_circ: 0.939585
Ran for 14050 frames Cell Events: 804 mean_circ: 0.93931
Ran for 15050 frames Cell Events: 860 mean_circ: 0.939297
Ran for 16550 frames Cell Events: 969 mean_circ: 0.938548
Ran for 17050 frames Cell Events: 994 mean_circ: 0.938781
Ran for 17550 frames Cell Events: 1024 mean_circ: 0.938502
Ran for 19050 frames Cell Events: 1126 mean_circ: 0.937864
Ran for 20050 frames Cell Events: 1187 mean_circ: 0.938251
Ran for 22050 frames Cell Events: 1309 mean_circ: 0.937938
Ran for 22550 frames Cell Events: 1330 mean_circ: 0.9381
Ran for 23550 frames Cell Events: 1399 mean_circ: 0.93774
Ran for 24050 frames Cell Events: 1421 mean_circ: 0.937792
Ran for 25050 frames Cell Events: 1496 mean_circ: 0.93791
Ran for 25550 frames Cell Events: 1520 mean_circ: 0.937901
Ran for 26550 frames Cell Events: 1574 mean_circ: 0.937848
Ran for 27550 frames Cell Events: 1646 mean_circ: 0.938009
Ran for 28550 frames Cell Events: 1697 mean_circ: 0.937919
Ran for 29550 frames Cell Events: 1758 mean_circ: 0.938233
Ran for 30050 frames Cell Events: 1789 mean_circ: 0.93838
Ran for 30550 frames Cell Events: 1813 mean_circ: 0.938491
Ran for 33050 frames Cell Events: 1985 mean_circ: 0.93873
Ran for 36050 frames Cell Events: 2184 mean_circ: 0.938865
Ran for 36550 frames Cell Events: 2216 mean_circ: 0.938949
Ran for 37550 frames Cell Events: 2279 mean_circ: 0.939041
Ran for 38050 frames Cell Events: 2304 mean_circ: 0.939114
Ran for 38550 frames Cell Events: 2327 mean_circ: 0.939135
Ran for 39050 frames Cell Events: 2352 mean_circ: 0.939166
Ran for 39550 frames Cell Events: 2379 mean_circ: 0.939185
Ran for 40050 frames Cell Events: 2402 mean_circ: 0.939099
Ran for 40550 frames Cell Events: 2430 mean_circ: 0.939084
Ran for 41550 frames Cell Events: 2481 mean_circ: 0.939163
Ran for 42050 frames Cell Events: 2508 mean_circ: 0.939093
Ran for 43550 frames Cell Events: 2600 mean_circ: 0.939013
Ran for 45550 frames Cell Events: 2747 mean_circ: 0.938996
Ran for 46050 frames Cell Events: 2774 mean_circ: 0.93912
Ran for 48050 frames Cell Events: 2925 mean_circ: 0.939328
Ran for 49050 frames Cell Events: 2966 mean_circ: 0.939261
Ran for 50550 frames Cell Events: 3039 mean_circ: 0.939322
Ran for 51550 frames Cell Events: 3078 mean_circ: 0.939331
Ran for 52050 frames Cell Events: 3095 mean_circ: 0.939294
Ran for 52550 frames Cell Events: 3115 mean_circ: 0.939418
Ran for 53550 frames Cell Events: 3168 mean_circ: 0.939455
Ran for 54050 frames Cell Events: 3193 mean_circ: 0.939469
Ran for 56550 frames Cell Events: 3336 mean_circ: 0.939441
Ran for 57050 frames Cell Events: 3360 mean_circ: 0.9394
Ran for 58550 frames Cell Events: 3423 mean_circ: 0.93937
Ran for 59050 frames Cell Events: 3455 mean_circ: 0.939441
Ran for 59550 frames Cell Events: 3483 mean_circ: 0.939443
Ran for 60550 frames Cell Events: 3556 mean_circ: 0.93954
Ran for 62550 frames Cell Events: 3683 mean_circ: 0.939538
Ran for 66050 frames Cell Events: 3947 mean_circ: 0.93944
Ran for 67550 frames Cell Events: 4048 mean_circ: 0.939446
Ran for 68050 frames Cell Events: 4073 mean_circ: 0.939495
Ran for 69050 frames Cell Events: 4122 mean_circ: 0.939519
Ran for 70050 frames Cell Events: 4174 mean_circ: 0.939504
Ran for 71550 frames Cell Events: 4244 mean_circ: 0.93957
Ran for 72550 frames Cell Events: 4285 mean_circ: 0.939495
Ran for 73050 frames Cell Events: 4315 mean_circ: 0.939398
Ran for 74050 frames Cell Events: 4394 mean_circ: 0.93924
Ran for 77050 frames Cell Events: 4580 mean_circ: 0.939376
Ran for 77550 frames Cell Events: 4618 mean_circ: 0.939362
Ran for 78550 frames Cell Events: 4676 mean_circ: 0.939359
Ran for 79050 frames Cell Events: 4709 mean_circ: 0.939336
Ran for 80050 frames Cell Events: 4754 mean_circ: 0.939336
Ran for 81050 frames Cell Events: 4818 mean_circ: 0.939409
Ran for 81550 frames Cell Events: 4850 mean_circ: 0.939424
Ran for 82050 frames Cell Events: 4872 mean_circ: 0.939422
Ran for 83050 frames Cell Events: 4930 mean_circ: 0.93941
Ran for 85550 frames Cell Events: 5111 mean_circ: 0.939283
Ran for 87550 frames Cell Events: 5256 mean_circ: 0.939132
Ran for 88050 frames Cell Events: 5290 mean_circ: 0.939025
Ran for 91050 frames Cell Events: 5483 mean_circ: 0.939197
Ran for 91550 frames Cell Events: 5524 mean_circ: 0.939241
Ran for 92050 frames Cell Events: 5543 mean_circ: 0.939257
Ran for 93050 frames Cell Events: 5610 mean_circ: 0.939232
Ran for 93550 frames Cell Events: 5650 mean_circ: 0.939211
Ran for 94050 frames Cell Events: 5678 mean_circ: 0.939221
Ran for 94550 frames Cell Events: 5701 mean_circ: 0.939276
Ran for 95550 frames Cell Events: 5771 mean_circ: 0.939183
Ran for 96550 frames Cell Events: 5836 mean_circ: 0.939115
Ran for 97050 frames Cell Events: 5864 mean_circ: 0.93905
Ran for 98550 frames Cell Events: 5945 mean_circ: 0.939035
Ran for 99050 frames Cell Events: 5965 mean_circ: 0.939038
Ran for 100050 frames Cell Events: 5991 mean_circ: 0.939038
Ran for 100550 frames Cell Events: 6025 mean_circ: 0.939033
Ran for 101550 frames Cell Events: 6085 mean_circ: 0.93907
Ran for 102050 frames Cell Events: 6108 mean_circ: 0.939065
Ran for 102550 frames Cell Events: 6138 mean_circ: 0.939099
Ran for 105050 frames Cell Events: 6310 mean_circ: 0.939136
Ran for 107050 frames Cell Events: 6395 mean_circ: 0.939237
Ran for 107550 frames Cell Events: 6423 mean_circ: 0.939284
Ran for 108550 frames Cell Events: 6477 mean_circ: 0.939292
Ran for 110050 frames Cell Events: 6565 mean_circ: 0.939187
Ran for 111050 frames Cell Events: 6646 mean_circ: 0.939177
Ran for 112050 frames Cell Events: 6708 mean_circ: 0.93917
Ran for 112550 frames Cell Events: 6742 mean_circ: 0.939156
Ran for 113050 frames Cell Events: 6775 mean_circ: 0.939165
Ran for 115550 frames Cell Events: 6888 mean_circ: 0.939157
Ran for 116050 frames Cell Events: 6924 mean_circ: 0.939119
Ran for 117050 frames Cell Events: 6986 mean_circ: 0.939122
Ran for 117550 frames Cell Events: 7013 mean_circ: 0.93912
Ran for 118050 frames Cell Events: 7048 mean_circ: 0.93915
Ran for 119050 frames Cell Events: 7116 mean_circ: 0.939162
Ran for 121050 frames Cell Events: 7235 mean_circ: 0.939152
Ran for 124550 frames Cell Events: 7455 mean_circ: 0.939198
Ran for 127050 frames Cell Events: 7609 mean_circ: 0.939321
Ran for 127550 frames Cell Events: 7642 mean_circ: 0.939335
Ran for 128050 frames Cell Events: 7661 mean_circ: 0.939344
Ran for 129050 frames Cell Events: 7720 mean_circ: 0.939372
Ran for 129550 frames Cell Events: 7744 mean_circ: 0.939368
Ran for 130050 frames Cell Events: 7773 mean_circ: 0.939393
Ran for 130550 frames Cell Events: 7795 mean_circ: 0.939397
Ran for 131050 frames Cell Events: 7816 mean_circ: 0.939377
Ran for 133050 frames Cell Events: 7948 mean_circ: 0.93942
Ran for 133550 frames Cell Events: 7982 mean_circ: 0.939359
Ran for 134050 frames Cell Events: 8018 mean_circ: 0.939334
Ran for 134550 frames Cell Events: 8050 mean_circ: 0.939363



End this measurment..



Stopping time: 4.71777e+008
****************************************
Measurement finished

Mean Circularitiy: 0.939354
Mean Area: 605.149
Mean FL-1 intensity: 3124.05
Mean FL-2 intensity: 3629.04
Mean FL-3 intensity: 0

frames processed: 132896
Total Time: 67527ms
Number of Runs (new bg img) 0
Frames collected: 134839
Skipped frames: 1943
Rescued Frames: 70930
Samples DAQ: 33709784
Samples written: 67419484
Samples per Channel: 33709742
FPS: 1996.82

Samples per second: 499204
Expected samples/s: 500000

Events: 8059
Events per second: 119.345
jumped back (PMT): 20
#Frames read, frequency
# 0 frames :207616
# 1 frames :49178
# 2 frames :3273
# 3 frames :1147
# 4 frames :518
# 5 frames :1203
# 6 frames :1908
# 7 frames :1040
# 8 frames :626
# 9 frames :497
# 10 frames :551
# 11 frames :395
# 12 frames :284
# 13 frames :247
# 14 frames :200
# 15 frames :138
# 16 frames :137
# 17 frames :112
# 18 frames :89
# 19 frames :57
# 20 frames :67
# 21 frames :54
# 22 frames :41
# 23 frames :24
# 24 frames :21
# 25 frames :26
# 26 frames :7
# 27 frames :19
# 28 frames :15
# 29 frames :21
# 30 frames :11
# 31 frames :12
# 32 frames :7
# 33 frames :5
# 34 frames :6
# 35 frames :2
# 36 frames :4
# 37 frames :1
# 38 frames :2
# 39 frames :5
# 40 frames :3
# 41 frames :1
# 42 frames :5
# 43 frames :3
# 44 frames :1
# 47 frames :2
# 49 frames :1
# 51 frames :1
# 52 frames :1
# 54 frames :1
# 61 frames :1
# 86 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1235
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0