Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_19.23.12 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "711ef7cd4b67c05b34ac296fb49da44c",
"name": "M1_20170619_1641_dsRNA_6_repeat_aft_clogging.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "17:23:37"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 10000 (max 10001) in
'10000'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.2431
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 4.80626e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1550 frames Cell Events: 88 mean_circ: 0.944685
Ran for 2050 frames Cell Events: 110 mean_circ: 0.946002
Ran for 2550 frames Cell Events: 127 mean_circ: 0.948064
Ran for 3050 frames Cell Events: 162 mean_circ: 0.948496
Ran for 3550 frames Cell Events: 198 mean_circ: 0.951174
Ran for 4550 frames Cell Events: 228 mean_circ: 0.951046
Ran for 5050 frames Cell Events: 278 mean_circ: 0.947877
Ran for 6050 frames Cell Events: 352 mean_circ: 0.944218
Ran for 7550 frames Cell Events: 478 mean_circ: 0.946062
Ran for 8050 frames Cell Events: 520 mean_circ: 0.946583
Ran for 9050 frames Cell Events: 585 mean_circ: 0.946435
Ran for 11050 frames Cell Events: 717 mean_circ: 0.94633
Ran for 11550 frames Cell Events: 745 mean_circ: 0.945753
Ran for 12050 frames Cell Events: 767 mean_circ: 0.946271
Ran for 12550 frames Cell Events: 792 mean_circ: 0.94635
Ran for 13050 frames Cell Events: 811 mean_circ: 0.945919
Ran for 13550 frames Cell Events: 842 mean_circ: 0.946055
Ran for 14050 frames Cell Events: 864 mean_circ: 0.946145
Ran for 14550 frames Cell Events: 889 mean_circ: 0.945663
Ran for 15050 frames Cell Events: 908 mean_circ: 0.945619
Ran for 15550 frames Cell Events: 933 mean_circ: 0.945674
Ran for 18550 frames Cell Events: 1120 mean_circ: 0.945681
Ran for 20050 frames Cell Events: 1224 mean_circ: 0.945489
Ran for 22550 frames Cell Events: 1393 mean_circ: 0.945449
Ran for 23050 frames Cell Events: 1425 mean_circ: 0.945616
Ran for 24050 frames Cell Events: 1483 mean_circ: 0.945681
Ran for 24550 frames Cell Events: 1513 mean_circ: 0.945604
Ran for 25050 frames Cell Events: 1546 mean_circ: 0.945499
Ran for 25550 frames Cell Events: 1577 mean_circ: 0.945474
Ran for 26050 frames Cell Events: 1620 mean_circ: 0.945569
Ran for 26550 frames Cell Events: 1656 mean_circ: 0.945672
Ran for 27050 frames Cell Events: 1689 mean_circ: 0.945894
Ran for 28050 frames Cell Events: 1761 mean_circ: 0.94577
Ran for 28550 frames Cell Events: 1797 mean_circ: 0.945952
Ran for 29550 frames Cell Events: 1870 mean_circ: 0.946211
Ran for 30050 frames Cell Events: 1903 mean_circ: 0.94613
Ran for 30550 frames Cell Events: 1926 mean_circ: 0.94631
Ran for 31050 frames Cell Events: 1954 mean_circ: 0.946422
Ran for 33050 frames Cell Events: 2073 mean_circ: 0.946465
Ran for 33550 frames Cell Events: 2101 mean_circ: 0.946559
Ran for 34050 frames Cell Events: 2130 mean_circ: 0.946644
Ran for 34550 frames Cell Events: 2166 mean_circ: 0.94657
Ran for 35050 frames Cell Events: 2206 mean_circ: 0.946449
Ran for 36050 frames Cell Events: 2265 mean_circ: 0.946373
Ran for 37050 frames Cell Events: 2310 mean_circ: 0.946299
Ran for 37550 frames Cell Events: 2333 mean_circ: 0.946311
Ran for 38050 frames Cell Events: 2354 mean_circ: 0.946149
Ran for 38550 frames Cell Events: 2375 mean_circ: 0.946026
Ran for 39550 frames Cell Events: 2430 mean_circ: 0.946123
Ran for 40550 frames Cell Events: 2489 mean_circ: 0.946078
Ran for 41050 frames Cell Events: 2515 mean_circ: 0.946101
Ran for 42050 frames Cell Events: 2548 mean_circ: 0.946105
Ran for 43050 frames Cell Events: 2618 mean_circ: 0.946092
Ran for 44050 frames Cell Events: 2699 mean_circ: 0.946227
Ran for 44550 frames Cell Events: 2724 mean_circ: 0.946294
Ran for 47050 frames Cell Events: 2913 mean_circ: 0.946258
Ran for 47550 frames Cell Events: 2931 mean_circ: 0.94624
Ran for 48050 frames Cell Events: 2965 mean_circ: 0.946192
Ran for 48550 frames Cell Events: 3004 mean_circ: 0.946128
Ran for 49050 frames Cell Events: 3037 mean_circ: 0.946248
Ran for 49550 frames Cell Events: 3066 mean_circ: 0.946284
Ran for 50050 frames Cell Events: 3080 mean_circ: 0.946175
Ran for 52050 frames Cell Events: 3208 mean_circ: 0.946123
Ran for 55050 frames Cell Events: 3421 mean_circ: 0.946036
Ran for 56050 frames Cell Events: 3476 mean_circ: 0.946108
Ran for 57550 frames Cell Events: 3600 mean_circ: 0.946211
Ran for 58050 frames Cell Events: 3620 mean_circ: 0.946149
Ran for 59050 frames Cell Events: 3684 mean_circ: 0.946219
Ran for 60050 frames Cell Events: 3732 mean_circ: 0.946252
Ran for 61550 frames Cell Events: 3829 mean_circ: 0.946177
Ran for 62050 frames Cell Events: 3865 mean_circ: 0.946141
Ran for 62550 frames Cell Events: 3894 mean_circ: 0.94602
Ran for 65050 frames Cell Events: 4063 mean_circ: 0.945911
Ran for 65550 frames Cell Events: 4093 mean_circ: 0.946003
Ran for 67050 frames Cell Events: 4176 mean_circ: 0.945848
Ran for 68050 frames Cell Events: 4246 mean_circ: 0.945953
Ran for 68550 frames Cell Events: 4280 mean_circ: 0.945997
Ran for 69050 frames Cell Events: 4313 mean_circ: 0.946095
Ran for 69550 frames Cell Events: 4352 mean_circ: 0.94604
Ran for 70050 frames Cell Events: 4382 mean_circ: 0.946068
Ran for 75050 frames Cell Events: 4705 mean_circ: 0.946356
Ran for 76050 frames Cell Events: 4775 mean_circ: 0.946439
Ran for 76550 frames Cell Events: 4807 mean_circ: 0.946423
Ran for 77550 frames Cell Events: 4859 mean_circ: 0.94657
Ran for 82550 frames Cell Events: 5183 mean_circ: 0.946766
Ran for 83550 frames Cell Events: 5231 mean_circ: 0.94664
Ran for 85050 frames Cell Events: 5322 mean_circ: 0.946692
Ran for 86050 frames Cell Events: 5385 mean_circ: 0.946725
Ran for 87050 frames Cell Events: 5451 mean_circ: 0.946818
Ran for 87550 frames Cell Events: 5491 mean_circ: 0.946796
Ran for 88050 frames Cell Events: 5523 mean_circ: 0.946807
Ran for 89050 frames Cell Events: 5586 mean_circ: 0.946813
Ran for 90550 frames Cell Events: 5697 mean_circ: 0.946976
Ran for 91050 frames Cell Events: 5720 mean_circ: 0.946903
Ran for 91550 frames Cell Events: 5740 mean_circ: 0.946894
Ran for 92550 frames Cell Events: 5801 mean_circ: 0.946877
Ran for 93550 frames Cell Events: 5866 mean_circ: 0.946874
Ran for 94550 frames Cell Events: 5939 mean_circ: 0.946831
Ran for 96550 frames Cell Events: 6109 mean_circ: 0.946784
Ran for 100050 frames Cell Events: 6335 mean_circ: 0.946607
Ran for 101050 frames Cell Events: 6375 mean_circ: 0.946593
Ran for 101550 frames Cell Events: 6398 mean_circ: 0.946626
Ran for 102550 frames Cell Events: 6466 mean_circ: 0.946544
Ran for 103050 frames Cell Events: 6487 mean_circ: 0.946584
Ran for 103550 frames Cell Events: 6512 mean_circ: 0.94659
Ran for 104050 frames Cell Events: 6537 mean_circ: 0.946616
Ran for 104550 frames Cell Events: 6556 mean_circ: 0.946591
Ran for 105050 frames Cell Events: 6595 mean_circ: 0.946586
Ran for 107550 frames Cell Events: 6764 mean_circ: 0.946617
Ran for 108050 frames Cell Events: 6793 mean_circ: 0.946663
Ran for 109050 frames Cell Events: 6874 mean_circ: 0.946643
Ran for 112550 frames Cell Events: 7068 mean_circ: 0.946746
Ran for 113050 frames Cell Events: 7105 mean_circ: 0.946776
Ran for 114050 frames Cell Events: 7192 mean_circ: 0.946766
Ran for 115550 frames Cell Events: 7283 mean_circ: 0.946723
Ran for 117050 frames Cell Events: 7405 mean_circ: 0.946704
Ran for 118050 frames Cell Events: 7470 mean_circ: 0.946759
Ran for 119550 frames Cell Events: 7577 mean_circ: 0.946795
Ran for 120050 frames Cell Events: 7599 mean_circ: 0.946764
Ran for 121050 frames Cell Events: 7689 mean_circ: 0.94681
Ran for 123050 frames Cell Events: 7804 mean_circ: 0.946882
Ran for 124050 frames Cell Events: 7870 mean_circ: 0.946844
Ran for 124550 frames Cell Events: 7897 mean_circ: 0.946858
Ran for 126050 frames Cell Events: 7968 mean_circ: 0.946729
Ran for 129050 frames Cell Events: 8149 mean_circ: 0.946779
Ran for 130550 frames Cell Events: 8254 mean_circ: 0.946798
Ran for 131050 frames Cell Events: 8277 mean_circ: 0.946792
Ran for 132050 frames Cell Events: 8330 mean_circ: 0.946873
Ran for 132550 frames Cell Events: 8361 mean_circ: 0.946838
Ran for 133050 frames Cell Events: 8387 mean_circ: 0.946875
Ran for 133550 frames Cell Events: 8422 mean_circ: 0.946842
Ran for 134550 frames Cell Events: 8490 mean_circ: 0.946816
Ran for 135050 frames Cell Events: 8520 mean_circ: 0.946828
Ran for 135550 frames Cell Events: 8552 mean_circ: 0.946777
Ran for 138550 frames Cell Events: 8772 mean_circ: 0.94678
Ran for 139050 frames Cell Events: 8814 mean_circ: 0.946758
Ran for 139550 frames Cell Events: 8848 mean_circ: 0.946774
Ran for 140050 frames Cell Events: 8875 mean_circ: 0.946795
Ran for 140550 frames Cell Events: 8906 mean_circ: 0.946807
Ran for 141550 frames Cell Events: 8960 mean_circ: 0.946825
Ran for 142550 frames Cell Events: 9015 mean_circ: 0.946804
Ran for 143550 frames Cell Events: 9085 mean_circ: 0.946839
Ran for 144050 frames Cell Events: 9126 mean_circ: 0.946871
Ran for 144550 frames Cell Events: 9148 mean_circ: 0.946863
Ran for 145050 frames Cell Events: 9173 mean_circ: 0.946878
Ran for 145550 frames Cell Events: 9199 mean_circ: 0.94687
Ran for 146050 frames Cell Events: 9227 mean_circ: 0.946873
Ran for 147050 frames Cell Events: 9292 mean_circ: 0.946814
Ran for 148550 frames Cell Events: 9387 mean_circ: 0.946839
Ran for 149050 frames Cell Events: 9416 mean_circ: 0.946852
Ran for 149550 frames Cell Events: 9434 mean_circ: 0.946806
Ran for 152050 frames Cell Events: 9596 mean_circ: 0.946835
Ran for 152550 frames Cell Events: 9618 mean_circ: 0.946843
Ran for 153050 frames Cell Events: 9650 mean_circ: 0.946796
Ran for 153550 frames Cell Events: 9672 mean_circ: 0.946807
Ran for 154550 frames Cell Events: 9731 mean_circ: 0.946818
Ran for 155550 frames Cell Events: 9782 mean_circ: 0.946843
Ran for 156550 frames Cell Events: 9830 mean_circ: 0.94684
Ran for 158050 frames Cell Events: 9903 mean_circ: 0.946828
Ran for 158550 frames Cell Events: 9938 mean_circ: 0.946812
Ran for 159050 frames Cell Events: 9979 mean_circ: 0.946764



End this measurment.
because stop condition (time/nr of cells) was met

Stopping time: 4.80706e+008
****************************************
Measurement finished

Mean Circularitiy: 0.946794
Mean Area: 613.703
Mean FL-1 intensity: 3308.91
Mean FL-2 intensity: 3717.46
Mean FL-3 intensity: 0

frames processed: 158353
Total Time: 79859.4ms
Number of Runs (new bg img) 0
Frames collected: 159518
Skipped frames: 14162
Rescued Frames: 2044530
Samples DAQ: 39879679
Samples written: 79758968
Samples per Channel: 39879484
FPS: 1997.48

Samples per second: 499371
Expected samples/s: 500000

Events: 10001
Events per second: 125.233
jumped back (PMT): 5120
#Frames read, frequency
# 0 frames :241242
# 1 frames :62934
# 2 frames :3545
# 3 frames :1392
# 4 frames :523
# 5 frames :2664
# 6 frames :1568
# 7 frames :1103
# 8 frames :640
# 9 frames :447
# 10 frames :518
# 11 frames :541
# 12 frames :448
# 13 frames :248
# 14 frames :198
# 15 frames :162
# 16 frames :118
# 17 frames :114
# 18 frames :103
# 19 frames :77
# 20 frames :60
# 21 frames :51
# 22 frames :37
# 23 frames :35
# 24 frames :28
# 25 frames :25
# 26 frames :25
# 27 frames :21
# 28 frames :13
# 29 frames :20
# 30 frames :11
# 31 frames :10
# 32 frames :11
# 33 frames :15
# 34 frames :11
# 35 frames :3
# 36 frames :3
# 37 frames :3
# 38 frames :3
# 39 frames :4
# 40 frames :1
# 41 frames :2
# 43 frames :1
# 44 frames :1
# 46 frames :2
# 47 frames :1
# 48 frames :3
# 49 frames :2
# 51 frames :1
# 53 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.2431
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0