Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_19.23.44 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "9a5d5d74922c3f20a4d804fd38418713",
"name": "M1_20170619_1644_dsRNA_U6_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "17:24:05"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8239 (max 8240) in '8239'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.2268
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 4.80807e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 2550 frames Cell Events: 154 mean_circ: 0.937282
Ran for 3550 frames Cell Events: 230 mean_circ: 0.934951
Ran for 5050 frames Cell Events: 338 mean_circ: 0.936023
Ran for 8550 frames Cell Events: 555 mean_circ: 0.93719
Ran for 9050 frames Cell Events: 577 mean_circ: 0.937383
Ran for 10050 frames Cell Events: 629 mean_circ: 0.937862
Ran for 10550 frames Cell Events: 660 mean_circ: 0.937413
Ran for 11050 frames Cell Events: 686 mean_circ: 0.937676
Ran for 11550 frames Cell Events: 714 mean_circ: 0.937205
Ran for 12050 frames Cell Events: 757 mean_circ: 0.937339
Ran for 13050 frames Cell Events: 816 mean_circ: 0.937707
Ran for 13550 frames Cell Events: 838 mean_circ: 0.937539
Ran for 15550 frames Cell Events: 977 mean_circ: 0.937289
Ran for 16550 frames Cell Events: 1036 mean_circ: 0.937477
Ran for 17550 frames Cell Events: 1095 mean_circ: 0.937583
Ran for 18050 frames Cell Events: 1119 mean_circ: 0.9378
Ran for 18550 frames Cell Events: 1150 mean_circ: 0.937664
Ran for 19050 frames Cell Events: 1172 mean_circ: 0.937719
Ran for 19550 frames Cell Events: 1195 mean_circ: 0.937514
Ran for 20050 frames Cell Events: 1230 mean_circ: 0.937204
Ran for 20550 frames Cell Events: 1245 mean_circ: 0.937376
Ran for 21050 frames Cell Events: 1267 mean_circ: 0.93758
Ran for 22050 frames Cell Events: 1318 mean_circ: 0.937612
Ran for 22550 frames Cell Events: 1337 mean_circ: 0.937409
Ran for 24050 frames Cell Events: 1417 mean_circ: 0.937387
Ran for 24550 frames Cell Events: 1435 mean_circ: 0.937175
Ran for 25050 frames Cell Events: 1466 mean_circ: 0.937128
Ran for 26050 frames Cell Events: 1527 mean_circ: 0.937039
Ran for 26550 frames Cell Events: 1557 mean_circ: 0.9369
Ran for 29050 frames Cell Events: 1705 mean_circ: 0.937346
Ran for 31550 frames Cell Events: 1869 mean_circ: 0.937635
Ran for 33550 frames Cell Events: 2015 mean_circ: 0.937332
Ran for 34050 frames Cell Events: 2049 mean_circ: 0.937222
Ran for 35050 frames Cell Events: 2103 mean_circ: 0.936948
Ran for 35550 frames Cell Events: 2139 mean_circ: 0.937035
Ran for 36050 frames Cell Events: 2180 mean_circ: 0.937065
Ran for 36550 frames Cell Events: 2213 mean_circ: 0.936947
Ran for 37550 frames Cell Events: 2283 mean_circ: 0.936911
Ran for 38550 frames Cell Events: 2341 mean_circ: 0.936878
Ran for 39050 frames Cell Events: 2370 mean_circ: 0.937068
Ran for 39550 frames Cell Events: 2401 mean_circ: 0.937021
Ran for 40050 frames Cell Events: 2427 mean_circ: 0.937095
Ran for 40550 frames Cell Events: 2451 mean_circ: 0.937106
Ran for 41550 frames Cell Events: 2517 mean_circ: 0.937194
Ran for 42050 frames Cell Events: 2554 mean_circ: 0.937164
Ran for 42550 frames Cell Events: 2576 mean_circ: 0.937066
Ran for 43050 frames Cell Events: 2604 mean_circ: 0.937131
Ran for 44550 frames Cell Events: 2690 mean_circ: 0.937342
Ran for 45050 frames Cell Events: 2710 mean_circ: 0.937199
Ran for 46050 frames Cell Events: 2773 mean_circ: 0.937055
Ran for 46550 frames Cell Events: 2798 mean_circ: 0.93699
Ran for 48050 frames Cell Events: 2898 mean_circ: 0.936976
Ran for 50050 frames Cell Events: 3032 mean_circ: 0.936976
Ran for 51050 frames Cell Events: 3095 mean_circ: 0.936836
Ran for 52050 frames Cell Events: 3164 mean_circ: 0.936887
Ran for 52550 frames Cell Events: 3188 mean_circ: 0.936865
Ran for 53550 frames Cell Events: 3244 mean_circ: 0.936769
Ran for 55550 frames Cell Events: 3350 mean_circ: 0.936744
Ran for 56550 frames Cell Events: 3403 mean_circ: 0.936848
Ran for 57050 frames Cell Events: 3428 mean_circ: 0.936866
Ran for 57550 frames Cell Events: 3460 mean_circ: 0.936893
Ran for 59050 frames Cell Events: 3541 mean_circ: 0.936574
Ran for 60050 frames Cell Events: 3593 mean_circ: 0.936668
Ran for 61050 frames Cell Events: 3661 mean_circ: 0.936827
Ran for 61550 frames Cell Events: 3682 mean_circ: 0.936766
Ran for 63050 frames Cell Events: 3776 mean_circ: 0.936831
Ran for 63550 frames Cell Events: 3793 mean_circ: 0.936893
Ran for 65050 frames Cell Events: 3869 mean_circ: 0.937051
Ran for 67050 frames Cell Events: 3964 mean_circ: 0.937126
Ran for 69050 frames Cell Events: 4061 mean_circ: 0.937122
Ran for 69550 frames Cell Events: 4088 mean_circ: 0.937185
Ran for 70050 frames Cell Events: 4112 mean_circ: 0.937211
Ran for 71050 frames Cell Events: 4162 mean_circ: 0.937103
Ran for 72550 frames Cell Events: 4222 mean_circ: 0.937167
Ran for 74550 frames Cell Events: 4316 mean_circ: 0.937235
Ran for 75050 frames Cell Events: 4356 mean_circ: 0.937198
Ran for 76550 frames Cell Events: 4444 mean_circ: 0.937089
Ran for 78550 frames Cell Events: 4542 mean_circ: 0.937018
Ran for 79050 frames Cell Events: 4576 mean_circ: 0.936903
Ran for 80050 frames Cell Events: 4633 mean_circ: 0.936811
Ran for 80550 frames Cell Events: 4668 mean_circ: 0.936917
Ran for 81050 frames Cell Events: 4688 mean_circ: 0.936945
Ran for 81550 frames Cell Events: 4716 mean_circ: 0.936949
Ran for 82050 frames Cell Events: 4745 mean_circ: 0.937
Ran for 82550 frames Cell Events: 4778 mean_circ: 0.937014
Ran for 83050 frames Cell Events: 4802 mean_circ: 0.937017
Ran for 84050 frames Cell Events: 4849 mean_circ: 0.937138
Ran for 84550 frames Cell Events: 4873 mean_circ: 0.937097
Ran for 85050 frames Cell Events: 4903 mean_circ: 0.937075
Ran for 85550 frames Cell Events: 4923 mean_circ: 0.937106
Ran for 86050 frames Cell Events: 4940 mean_circ: 0.937119
Ran for 86550 frames Cell Events: 4955 mean_circ: 0.937064
Ran for 87050 frames Cell Events: 4977 mean_circ: 0.937098
Ran for 87550 frames Cell Events: 5000 mean_circ: 0.937083
Ran for 88050 frames Cell Events: 5028 mean_circ: 0.937074
Ran for 88550 frames Cell Events: 5052 mean_circ: 0.936989
Ran for 89050 frames Cell Events: 5078 mean_circ: 0.936881
Ran for 89550 frames Cell Events: 5102 mean_circ: 0.936881
Ran for 91550 frames Cell Events: 5203 mean_circ: 0.936859
Ran for 92050 frames Cell Events: 5222 mean_circ: 0.936872
Ran for 93050 frames Cell Events: 5279 mean_circ: 0.936884
Ran for 94050 frames Cell Events: 5343 mean_circ: 0.936759
Ran for 95050 frames Cell Events: 5389 mean_circ: 0.936723
Ran for 96050 frames Cell Events: 5445 mean_circ: 0.936691
Ran for 96550 frames Cell Events: 5468 mean_circ: 0.936666
Ran for 97050 frames Cell Events: 5491 mean_circ: 0.936629
Ran for 97550 frames Cell Events: 5529 mean_circ: 0.936653
Ran for 98050 frames Cell Events: 5562 mean_circ: 0.936649
Ran for 98550 frames Cell Events: 5594 mean_circ: 0.936627
Ran for 99550 frames Cell Events: 5657 mean_circ: 0.936616
Ran for 100050 frames Cell Events: 5675 mean_circ: 0.936571
Ran for 101050 frames Cell Events: 5734 mean_circ: 0.936594
Ran for 101550 frames Cell Events: 5765 mean_circ: 0.936603
Ran for 102050 frames Cell Events: 5786 mean_circ: 0.936579
Ran for 103050 frames Cell Events: 5834 mean_circ: 0.936593
Ran for 103550 frames Cell Events: 5857 mean_circ: 0.936585
Ran for 104550 frames Cell Events: 5911 mean_circ: 0.936615
Ran for 105050 frames Cell Events: 5938 mean_circ: 0.936587
Ran for 105550 frames Cell Events: 5971 mean_circ: 0.936532
Ran for 107550 frames Cell Events: 6099 mean_circ: 0.936541
Ran for 108050 frames Cell Events: 6129 mean_circ: 0.936583
Ran for 109050 frames Cell Events: 6188 mean_circ: 0.936658
Ran for 109550 frames Cell Events: 6212 mean_circ: 0.936661
Ran for 110050 frames Cell Events: 6252 mean_circ: 0.936665
Ran for 110550 frames Cell Events: 6285 mean_circ: 0.936702
Ran for 111550 frames Cell Events: 6347 mean_circ: 0.936603
Ran for 113550 frames Cell Events: 6482 mean_circ: 0.936685
Ran for 115550 frames Cell Events: 6628 mean_circ: 0.936718
Ran for 116050 frames Cell Events: 6664 mean_circ: 0.936693
Ran for 117050 frames Cell Events: 6737 mean_circ: 0.936701
Ran for 117550 frames Cell Events: 6762 mean_circ: 0.936629
Ran for 118050 frames Cell Events: 6797 mean_circ: 0.936633
Ran for 118550 frames Cell Events: 6822 mean_circ: 0.936591
Ran for 119550 frames Cell Events: 6871 mean_circ: 0.936634
Ran for 120050 frames Cell Events: 6905 mean_circ: 0.936649
Ran for 120550 frames Cell Events: 6933 mean_circ: 0.936595
Ran for 121050 frames Cell Events: 6960 mean_circ: 0.936591
Ran for 121550 frames Cell Events: 6992 mean_circ: 0.936624
Ran for 123050 frames Cell Events: 7105 mean_circ: 0.936538
Ran for 126050 frames Cell Events: 7293 mean_circ: 0.936442
Ran for 126550 frames Cell Events: 7322 mean_circ: 0.936379
Ran for 127050 frames Cell Events: 7355 mean_circ: 0.936338
Ran for 127550 frames Cell Events: 7380 mean_circ: 0.936356
Ran for 128550 frames Cell Events: 7454 mean_circ: 0.936322
Ran for 130550 frames Cell Events: 7570 mean_circ: 0.936216
Ran for 131550 frames Cell Events: 7622 mean_circ: 0.936271
Ran for 132050 frames Cell Events: 7651 mean_circ: 0.936309
Ran for 132550 frames Cell Events: 7672 mean_circ: 0.936285
Ran for 134050 frames Cell Events: 7766 mean_circ: 0.93626
Ran for 135050 frames Cell Events: 7828 mean_circ: 0.936146
Ran for 135550 frames Cell Events: 7859 mean_circ: 0.936233
Ran for 136050 frames Cell Events: 7895 mean_circ: 0.936259
Ran for 136550 frames Cell Events: 7912 mean_circ: 0.936281
Ran for 137050 frames Cell Events: 7935 mean_circ: 0.936301
Ran for 137550 frames Cell Events: 7956 mean_circ: 0.936302
Ran for 138050 frames Cell Events: 7983 mean_circ: 0.936277
Ran for 138550 frames Cell Events: 8004 mean_circ: 0.936309
Ran for 139050 frames Cell Events: 8033 mean_circ: 0.936312
Ran for 140050 frames Cell Events: 8096 mean_circ: 0.9362
Ran for 140550 frames Cell Events: 8117 mean_circ: 0.936157
Ran for 141550 frames Cell Events: 8175 mean_circ: 0.936181
Ran for 142550 frames Cell Events: 8214 mean_circ: 0.936171
Ran for 143050 frames Cell Events: 8236 mean_circ: 0.936142



End this measurment..



Stopping time: 4.80878e+008
****************************************
Measurement finished

Mean Circularitiy: 0.936162
Mean Area: 610.645
Mean FL-1 intensity: 3101.32
Mean FL-2 intensity: 3948.1
Mean FL-3 intensity: 0

frames processed: 142955
Total Time: 71650.5ms
Number of Runs (new bg img) 0
Frames collected: 143147
Skipped frames: 14354
Rescued Frames: 2115985
Samples DAQ: 35786673
Samples written: 71573234
Samples per Channel: 35786617
FPS: 1997.85

Samples per second: 499461
Expected samples/s: 500000

Events: 8240
Events per second: 115.003
jumped back (PMT): 14
#Frames read, frequency
# 0 frames :214717
# 1 frames :58030
# 2 frames :3427
# 3 frames :1243
# 4 frames :546
# 5 frames :2135
# 6 frames :1417
# 7 frames :1060
# 8 frames :559
# 9 frames :453
# 10 frames :615
# 11 frames :434
# 12 frames :353
# 13 frames :253
# 14 frames :180
# 15 frames :146
# 16 frames :103
# 17 frames :96
# 18 frames :81
# 19 frames :48
# 20 frames :53
# 21 frames :43
# 22 frames :37
# 23 frames :19
# 24 frames :15
# 25 frames :24
# 26 frames :23
# 27 frames :15
# 28 frames :11
# 29 frames :14
# 30 frames :14
# 31 frames :9
# 32 frames :9
# 33 frames :7
# 34 frames :5
# 35 frames :3
# 36 frames :2
# 37 frames :4
# 38 frames :2
# 40 frames :2
# 41 frames :2
# 43 frames :2
# 44 frames :1
# 45 frames :1
# 46 frames :3
# 48 frames :1
# 52 frames :1
# 59 frames :1
# 81 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.2268
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0