Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_19.24.13 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "ff489dcda2867af114a7b45babb3241c",
"name": "M1_20170619_1656_dsRNA_U4_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "17:24:36"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9121 (max 9122) in '9121'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1386
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 4.81508e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 2050 frames Cell Events: 103 mean_circ: 0.949936
Ran for 2550 frames Cell Events: 132 mean_circ: 0.949936
Ran for 5550 frames Cell Events: 348 mean_circ: 0.950734
Ran for 6550 frames Cell Events: 413 mean_circ: 0.95123
Ran for 7550 frames Cell Events: 471 mean_circ: 0.951933
Ran for 8050 frames Cell Events: 507 mean_circ: 0.952403
Ran for 9050 frames Cell Events: 599 mean_circ: 0.950681
Ran for 9550 frames Cell Events: 639 mean_circ: 0.951266
Ran for 10550 frames Cell Events: 679 mean_circ: 0.95088
Ran for 11550 frames Cell Events: 717 mean_circ: 0.950684
Ran for 12050 frames Cell Events: 752 mean_circ: 0.950769
Ran for 13050 frames Cell Events: 796 mean_circ: 0.949859
Ran for 14050 frames Cell Events: 849 mean_circ: 0.949921
Ran for 15050 frames Cell Events: 895 mean_circ: 0.950255
Ran for 16050 frames Cell Events: 930 mean_circ: 0.949737
Ran for 16550 frames Cell Events: 967 mean_circ: 0.949302
Ran for 17050 frames Cell Events: 1001 mean_circ: 0.949443
Ran for 17550 frames Cell Events: 1027 mean_circ: 0.949394
Ran for 18050 frames Cell Events: 1049 mean_circ: 0.949356
Ran for 18550 frames Cell Events: 1078 mean_circ: 0.948745
Ran for 19050 frames Cell Events: 1111 mean_circ: 0.948582
Ran for 20050 frames Cell Events: 1164 mean_circ: 0.948238
Ran for 21050 frames Cell Events: 1219 mean_circ: 0.947856
Ran for 22050 frames Cell Events: 1293 mean_circ: 0.948051
Ran for 22550 frames Cell Events: 1333 mean_circ: 0.948252
Ran for 23050 frames Cell Events: 1366 mean_circ: 0.948273
Ran for 23550 frames Cell Events: 1411 mean_circ: 0.948213
Ran for 25550 frames Cell Events: 1549 mean_circ: 0.947609
Ran for 27050 frames Cell Events: 1664 mean_circ: 0.94773
Ran for 29550 frames Cell Events: 1795 mean_circ: 0.947303
Ran for 31050 frames Cell Events: 1862 mean_circ: 0.947211
Ran for 35050 frames Cell Events: 2120 mean_circ: 0.947579
Ran for 35550 frames Cell Events: 2159 mean_circ: 0.947643
Ran for 36550 frames Cell Events: 2208 mean_circ: 0.947577
Ran for 37050 frames Cell Events: 2239 mean_circ: 0.947346
Ran for 38050 frames Cell Events: 2280 mean_circ: 0.94733
Ran for 39550 frames Cell Events: 2357 mean_circ: 0.94719
Ran for 40050 frames Cell Events: 2373 mean_circ: 0.947161
Ran for 41050 frames Cell Events: 2419 mean_circ: 0.947191
Ran for 41550 frames Cell Events: 2447 mean_circ: 0.947213
Ran for 42050 frames Cell Events: 2473 mean_circ: 0.947196
Ran for 42550 frames Cell Events: 2503 mean_circ: 0.946987
Ran for 43550 frames Cell Events: 2567 mean_circ: 0.946988
Ran for 44550 frames Cell Events: 2666 mean_circ: 0.947052
Ran for 45050 frames Cell Events: 2698 mean_circ: 0.947028
Ran for 46050 frames Cell Events: 2769 mean_circ: 0.947126
Ran for 46550 frames Cell Events: 2792 mean_circ: 0.946929
Ran for 47050 frames Cell Events: 2820 mean_circ: 0.946807
Ran for 48050 frames Cell Events: 2890 mean_circ: 0.946855
Ran for 50050 frames Cell Events: 2983 mean_circ: 0.946792
Ran for 50550 frames Cell Events: 3010 mean_circ: 0.946793
Ran for 51050 frames Cell Events: 3027 mean_circ: 0.946671
Ran for 51550 frames Cell Events: 3059 mean_circ: 0.946577
Ran for 52550 frames Cell Events: 3128 mean_circ: 0.946439
Ran for 53550 frames Cell Events: 3174 mean_circ: 0.946276
Ran for 54050 frames Cell Events: 3186 mean_circ: 0.946234
Ran for 54550 frames Cell Events: 3207 mean_circ: 0.946208
Ran for 55050 frames Cell Events: 3220 mean_circ: 0.946244
Ran for 55550 frames Cell Events: 3240 mean_circ: 0.946275
Ran for 56050 frames Cell Events: 3262 mean_circ: 0.946347
Ran for 56550 frames Cell Events: 3286 mean_circ: 0.946462
Ran for 58550 frames Cell Events: 3375 mean_circ: 0.946518
Ran for 59050 frames Cell Events: 3404 mean_circ: 0.946354
Ran for 60550 frames Cell Events: 3484 mean_circ: 0.946385
Ran for 61550 frames Cell Events: 3550 mean_circ: 0.946422
Ran for 63050 frames Cell Events: 3637 mean_circ: 0.946346
Ran for 63550 frames Cell Events: 3656 mean_circ: 0.946373
Ran for 65050 frames Cell Events: 3739 mean_circ: 0.946393
Ran for 65550 frames Cell Events: 3755 mean_circ: 0.946408
Ran for 66050 frames Cell Events: 3784 mean_circ: 0.946506
Ran for 66550 frames Cell Events: 3808 mean_circ: 0.946528
Ran for 67550 frames Cell Events: 3866 mean_circ: 0.946612
Ran for 68050 frames Cell Events: 3894 mean_circ: 0.946555
Ran for 68550 frames Cell Events: 3916 mean_circ: 0.946491
Ran for 69050 frames Cell Events: 3936 mean_circ: 0.946465
Ran for 69550 frames Cell Events: 3966 mean_circ: 0.946454
Ran for 70050 frames Cell Events: 3990 mean_circ: 0.946464
Ran for 70550 frames Cell Events: 4015 mean_circ: 0.946369
Ran for 71050 frames Cell Events: 4039 mean_circ: 0.9464
Ran for 71550 frames Cell Events: 4069 mean_circ: 0.946273
Ran for 72050 frames Cell Events: 4089 mean_circ: 0.946256
Ran for 73050 frames Cell Events: 4145 mean_circ: 0.94621
Ran for 73550 frames Cell Events: 4175 mean_circ: 0.946219
Ran for 74050 frames Cell Events: 4204 mean_circ: 0.946278
Ran for 75050 frames Cell Events: 4251 mean_circ: 0.946231
Ran for 76050 frames Cell Events: 4319 mean_circ: 0.946137
Ran for 76550 frames Cell Events: 4334 mean_circ: 0.946172
Ran for 77050 frames Cell Events: 4355 mean_circ: 0.946162
Ran for 77550 frames Cell Events: 4385 mean_circ: 0.946087
Ran for 78550 frames Cell Events: 4429 mean_circ: 0.946076
Ran for 79550 frames Cell Events: 4485 mean_circ: 0.946154
Ran for 80050 frames Cell Events: 4505 mean_circ: 0.946176
Ran for 80550 frames Cell Events: 4529 mean_circ: 0.946162
Ran for 81050 frames Cell Events: 4568 mean_circ: 0.946201
Ran for 81550 frames Cell Events: 4606 mean_circ: 0.946199
Ran for 82050 frames Cell Events: 4649 mean_circ: 0.946211
Ran for 83050 frames Cell Events: 4705 mean_circ: 0.946219
Ran for 84550 frames Cell Events: 4800 mean_circ: 0.946277
Ran for 85050 frames Cell Events: 4826 mean_circ: 0.946313
Ran for 86050 frames Cell Events: 4884 mean_circ: 0.946294
Ran for 86550 frames Cell Events: 4901 mean_circ: 0.946282
Ran for 90050 frames Cell Events: 5176 mean_circ: 0.946373
Ran for 90550 frames Cell Events: 5209 mean_circ: 0.946419
Ran for 92050 frames Cell Events: 5339 mean_circ: 0.946509
Ran for 93550 frames Cell Events: 5437 mean_circ: 0.946494
Ran for 94050 frames Cell Events: 5452 mean_circ: 0.946564
Ran for 94550 frames Cell Events: 5485 mean_circ: 0.946624
Ran for 95550 frames Cell Events: 5521 mean_circ: 0.946597
Ran for 96050 frames Cell Events: 5564 mean_circ: 0.946562
Ran for 96550 frames Cell Events: 5598 mean_circ: 0.946554
Ran for 97050 frames Cell Events: 5622 mean_circ: 0.94658
Ran for 97550 frames Cell Events: 5648 mean_circ: 0.946519
Ran for 98550 frames Cell Events: 5722 mean_circ: 0.946527
Ran for 99550 frames Cell Events: 5794 mean_circ: 0.94656
Ran for 100050 frames Cell Events: 5819 mean_circ: 0.946543
Ran for 100550 frames Cell Events: 5851 mean_circ: 0.94653
Ran for 102050 frames Cell Events: 5944 mean_circ: 0.946539
Ran for 102550 frames Cell Events: 5971 mean_circ: 0.946541
Ran for 103050 frames Cell Events: 6003 mean_circ: 0.946493
Ran for 103550 frames Cell Events: 6040 mean_circ: 0.946506
Ran for 104050 frames Cell Events: 6063 mean_circ: 0.946528
Ran for 104550 frames Cell Events: 6102 mean_circ: 0.946545
Ran for 105050 frames Cell Events: 6147 mean_circ: 0.946604
Ran for 106550 frames Cell Events: 6281 mean_circ: 0.946581
Ran for 107050 frames Cell Events: 6319 mean_circ: 0.946478
Ran for 108050 frames Cell Events: 6375 mean_circ: 0.946486
Ran for 108550 frames Cell Events: 6400 mean_circ: 0.946497
Ran for 109550 frames Cell Events: 6460 mean_circ: 0.946524
Ran for 110550 frames Cell Events: 6499 mean_circ: 0.946452
Ran for 111550 frames Cell Events: 6555 mean_circ: 0.946439
Ran for 112050 frames Cell Events: 6596 mean_circ: 0.946458
Ran for 112550 frames Cell Events: 6624 mean_circ: 0.946446
Ran for 116050 frames Cell Events: 6891 mean_circ: 0.946306
Ran for 118550 frames Cell Events: 7061 mean_circ: 0.946418
Ran for 119050 frames Cell Events: 7085 mean_circ: 0.946411
Ran for 119550 frames Cell Events: 7122 mean_circ: 0.9464
Ran for 120050 frames Cell Events: 7160 mean_circ: 0.9464
Ran for 122050 frames Cell Events: 7338 mean_circ: 0.946275
Ran for 123550 frames Cell Events: 7454 mean_circ: 0.946133
Ran for 124550 frames Cell Events: 7528 mean_circ: 0.946143
Ran for 125550 frames Cell Events: 7582 mean_circ: 0.946115
Ran for 126550 frames Cell Events: 7652 mean_circ: 0.946164
Ran for 127050 frames Cell Events: 7687 mean_circ: 0.946162
Ran for 128050 frames Cell Events: 7763 mean_circ: 0.946259
Ran for 128550 frames Cell Events: 7784 mean_circ: 0.946283
Ran for 129050 frames Cell Events: 7812 mean_circ: 0.946257
Ran for 130050 frames Cell Events: 7890 mean_circ: 0.946183
Ran for 131550 frames Cell Events: 7996 mean_circ: 0.946126
Ran for 133050 frames Cell Events: 8117 mean_circ: 0.946171
Ran for 136050 frames Cell Events: 8321 mean_circ: 0.946077
Ran for 136550 frames Cell Events: 8358 mean_circ: 0.946075
Ran for 138550 frames Cell Events: 8513 mean_circ: 0.946013
Ran for 140050 frames Cell Events: 8631 mean_circ: 0.946004
Ran for 140550 frames Cell Events: 8669 mean_circ: 0.945956
Ran for 142050 frames Cell Events: 8782 mean_circ: 0.94588
Ran for 143050 frames Cell Events: 8855 mean_circ: 0.945911
Ran for 143550 frames Cell Events: 8889 mean_circ: 0.94591
Ran for 146050 frames Cell Events: 9050 mean_circ: 0.945893
Ran for 147050 frames Cell Events: 9112 mean_circ: 0.945898



End this measurment..



Stopping time: 4.81582e+008
****************************************
Measurement finished

Mean Circularitiy: 0.945877
Mean Area: 632.536
Mean FL-1 intensity: 4017.33
Mean FL-2 intensity: 3851.96
Mean FL-3 intensity: 0

frames processed: 146982
Total Time: 73740.2ms
Number of Runs (new bg img) 0
Frames collected: 147259
Skipped frames: 14631
Rescued Frames: 2191797
Samples DAQ: 36816117
Samples written: 73629620
Samples per Channel: 36814810
FPS: 1997

Samples per second: 499250
Expected samples/s: 500000

Events: 9122
Events per second: 123.705
jumped back (PMT): 4648
#Frames read, frequency
# 0 frames :223294
# 1 frames :57298
# 2 frames :3506
# 3 frames :1359
# 4 frames :503
# 5 frames :2229
# 6 frames :1368
# 7 frames :1016
# 8 frames :582
# 9 frames :396
# 10 frames :695
# 11 frames :423
# 12 frames :360
# 13 frames :226
# 14 frames :186
# 15 frames :165
# 16 frames :154
# 17 frames :120
# 18 frames :104
# 19 frames :76
# 20 frames :64
# 21 frames :47
# 22 frames :52
# 23 frames :46
# 24 frames :25
# 25 frames :29
# 26 frames :21
# 27 frames :23
# 28 frames :13
# 29 frames :12
# 30 frames :10
# 31 frames :8
# 32 frames :17
# 33 frames :9
# 34 frames :4
# 35 frames :3
# 36 frames :8
# 37 frames :2
# 38 frames :1
# 39 frames :2
# 41 frames :1
# 42 frames :1
# 43 frames :1
# 44 frames :1
# 45 frames :1
# 46 frames :1
# 47 frames :1
# 49 frames :1
# 52 frames :1
# 53 frames :2
# 59 frames :1
# 60 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1386
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0