Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_19.34.39 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "bc3e6b68d89148faa7a8c1c61284cb01",
"name": "M1_20170620_1419_dsRNA_W2_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "17:35:03"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9295 (max 9297) in '9295'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.5154
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 5.58499e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 34 mean_circ: 0.906821
Ran for 1050 frames Cell Events: 60 mean_circ: 0.917588
Ran for 2050 frames Cell Events: 115 mean_circ: 0.931938
Ran for 2550 frames Cell Events: 145 mean_circ: 0.933588
Ran for 5050 frames Cell Events: 326 mean_circ: 0.935909
Ran for 6050 frames Cell Events: 411 mean_circ: 0.936189
Ran for 7050 frames Cell Events: 493 mean_circ: 0.936125
Ran for 8050 frames Cell Events: 575 mean_circ: 0.935848
Ran for 11550 frames Cell Events: 801 mean_circ: 0.935016
Ran for 14550 frames Cell Events: 977 mean_circ: 0.934234
Ran for 15050 frames Cell Events: 1007 mean_circ: 0.933811
Ran for 18050 frames Cell Events: 1245 mean_circ: 0.933298
Ran for 18550 frames Cell Events: 1274 mean_circ: 0.932687
Ran for 19550 frames Cell Events: 1347 mean_circ: 0.932693
Ran for 21050 frames Cell Events: 1432 mean_circ: 0.933197
Ran for 21550 frames Cell Events: 1463 mean_circ: 0.933126
Ran for 22050 frames Cell Events: 1504 mean_circ: 0.932813
Ran for 22550 frames Cell Events: 1539 mean_circ: 0.932391
Ran for 23050 frames Cell Events: 1568 mean_circ: 0.932448
Ran for 24050 frames Cell Events: 1649 mean_circ: 0.932544
Ran for 25050 frames Cell Events: 1707 mean_circ: 0.932209
Ran for 27050 frames Cell Events: 1846 mean_circ: 0.932454
Ran for 27550 frames Cell Events: 1879 mean_circ: 0.932589
Ran for 29050 frames Cell Events: 1963 mean_circ: 0.932764
Ran for 29550 frames Cell Events: 1987 mean_circ: 0.932444
Ran for 30050 frames Cell Events: 2004 mean_circ: 0.932446
Ran for 31050 frames Cell Events: 2052 mean_circ: 0.93247
Ran for 32550 frames Cell Events: 2151 mean_circ: 0.932646
Ran for 33550 frames Cell Events: 2218 mean_circ: 0.932785
Ran for 35550 frames Cell Events: 2377 mean_circ: 0.932723
Ran for 36050 frames Cell Events: 2410 mean_circ: 0.932541
Ran for 39550 frames Cell Events: 2673 mean_circ: 0.932471
Ran for 40050 frames Cell Events: 2691 mean_circ: 0.932526
Ran for 42050 frames Cell Events: 2844 mean_circ: 0.932992
Ran for 42550 frames Cell Events: 2878 mean_circ: 0.932988
Ran for 43550 frames Cell Events: 2956 mean_circ: 0.933049
Ran for 44550 frames Cell Events: 3022 mean_circ: 0.933102
Ran for 45050 frames Cell Events: 3048 mean_circ: 0.933185
Ran for 45550 frames Cell Events: 3083 mean_circ: 0.933151
Ran for 46050 frames Cell Events: 3102 mean_circ: 0.933177
Ran for 49050 frames Cell Events: 3286 mean_circ: 0.933096
Ran for 51550 frames Cell Events: 3439 mean_circ: 0.933262
Ran for 52050 frames Cell Events: 3472 mean_circ: 0.933168
Ran for 52550 frames Cell Events: 3492 mean_circ: 0.933156
Ran for 53550 frames Cell Events: 3549 mean_circ: 0.933207
Ran for 54550 frames Cell Events: 3620 mean_circ: 0.933207
Ran for 55550 frames Cell Events: 3687 mean_circ: 0.933318
Ran for 58550 frames Cell Events: 3893 mean_circ: 0.933344
Ran for 59050 frames Cell Events: 3912 mean_circ: 0.933349
Ran for 60050 frames Cell Events: 3990 mean_circ: 0.933298
Ran for 60550 frames Cell Events: 4016 mean_circ: 0.933289
Ran for 61050 frames Cell Events: 4034 mean_circ: 0.933272
Ran for 63050 frames Cell Events: 4148 mean_circ: 0.933233
Ran for 64050 frames Cell Events: 4234 mean_circ: 0.933229
Ran for 68050 frames Cell Events: 4519 mean_circ: 0.933146
Ran for 73050 frames Cell Events: 4852 mean_circ: 0.933083
Ran for 73550 frames Cell Events: 4879 mean_circ: 0.933083
Ran for 74550 frames Cell Events: 4928 mean_circ: 0.933092
Ran for 75050 frames Cell Events: 4966 mean_circ: 0.933144
Ran for 75550 frames Cell Events: 4995 mean_circ: 0.933083
Ran for 80550 frames Cell Events: 5348 mean_circ: 0.932927
Ran for 81050 frames Cell Events: 5383 mean_circ: 0.932927
Ran for 83050 frames Cell Events: 5528 mean_circ: 0.932707
Ran for 84550 frames Cell Events: 5607 mean_circ: 0.932673
Ran for 85550 frames Cell Events: 5657 mean_circ: 0.932623
Ran for 87050 frames Cell Events: 5757 mean_circ: 0.932667
Ran for 88050 frames Cell Events: 5810 mean_circ: 0.932708
Ran for 92050 frames Cell Events: 6069 mean_circ: 0.932535
Ran for 92550 frames Cell Events: 6093 mean_circ: 0.93256
Ran for 93050 frames Cell Events: 6134 mean_circ: 0.93256
Ran for 94550 frames Cell Events: 6214 mean_circ: 0.932458
Ran for 96550 frames Cell Events: 6356 mean_circ: 0.932436
Ran for 98050 frames Cell Events: 6457 mean_circ: 0.932326
Ran for 98550 frames Cell Events: 6489 mean_circ: 0.932348
Ran for 101550 frames Cell Events: 6704 mean_circ: 0.932232
Ran for 107550 frames Cell Events: 7148 mean_circ: 0.93219
Ran for 108550 frames Cell Events: 7217 mean_circ: 0.932142
Ran for 110050 frames Cell Events: 7347 mean_circ: 0.932152
Ran for 110550 frames Cell Events: 7371 mean_circ: 0.932141
Ran for 111550 frames Cell Events: 7433 mean_circ: 0.932144
Ran for 112550 frames Cell Events: 7507 mean_circ: 0.932227
Ran for 113550 frames Cell Events: 7585 mean_circ: 0.932308
Ran for 116050 frames Cell Events: 7761 mean_circ: 0.93222
Ran for 116550 frames Cell Events: 7796 mean_circ: 0.932246
Ran for 118550 frames Cell Events: 7936 mean_circ: 0.932242
Ran for 120050 frames Cell Events: 8046 mean_circ: 0.932339
Ran for 122550 frames Cell Events: 8216 mean_circ: 0.932425
Ran for 123050 frames Cell Events: 8258 mean_circ: 0.932433
Ran for 125550 frames Cell Events: 8435 mean_circ: 0.932576
Ran for 126050 frames Cell Events: 8469 mean_circ: 0.932576
Ran for 133550 frames Cell Events: 8995 mean_circ: 0.932598
Ran for 134050 frames Cell Events: 9017 mean_circ: 0.93255
Ran for 135550 frames Cell Events: 9126 mean_circ: 0.932608



End this measurment..



Stopping time: 5.58568e+008
****************************************
Measurement finished

Mean Circularitiy: 0.932664
Mean Area: 641.576
Mean FL-1 intensity: 3768.8
Mean FL-2 intensity: 3310.26
Mean FL-3 intensity: 0

frames processed: 135003
Total Time: 69051.3ms
Number of Runs (new bg img) 0
Frames collected: 137924
Skipped frames: 6949
Rescued Frames: 1034140
Samples DAQ: 34480912
Samples written: 68961722
Samples per Channel: 34480861
FPS: 1997.41

Samples per second: 499351
Expected samples/s: 500000

Events: 9297
Events per second: 134.639
jumped back (PMT): 23
#Frames read, frequency
# 0 frames :224342
# 1 frames :32816
# 2 frames :2433
# 3 frames :973
# 4 frames :636
# 5 frames :349
# 6 frames :1397
# 7 frames :985
# 8 frames :734
# 9 frames :584
# 10 frames :550
# 11 frames :411
# 12 frames :320
# 13 frames :221
# 14 frames :226
# 15 frames :253
# 16 frames :216
# 17 frames :165
# 18 frames :142
# 19 frames :111
# 20 frames :110
# 21 frames :100
# 22 frames :85
# 23 frames :81
# 24 frames :81
# 25 frames :69
# 26 frames :57
# 27 frames :65
# 28 frames :36
# 29 frames :45
# 30 frames :22
# 31 frames :32
# 32 frames :37
# 33 frames :34
# 34 frames :22
# 35 frames :21
# 36 frames :22
# 37 frames :11
# 38 frames :15
# 39 frames :15
# 40 frames :14
# 41 frames :11
# 42 frames :9
# 43 frames :8
# 44 frames :15
# 45 frames :6
# 46 frames :12
# 47 frames :3
# 48 frames :6
# 49 frames :2
# 50 frames :6
# 51 frames :3
# 52 frames :3
# 53 frames :3
# 54 frames :5
# 55 frames :5
# 56 frames :4
# 58 frames :2
# 59 frames :3
# 60 frames :1
# 61 frames :2
# 62 frames :2
# 63 frames :1
# 64 frames :4
# 67 frames :1
# 70 frames :2
# 77 frames :2
# 85 frames :2
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.5154
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0