Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_19.36.08 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "931c5d4ef250b48adbac2da677105d07",
"name": "M1_20170620_1442_dsRNA_T4_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "17:36:34"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 10000 (max 10001) in
'10000'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.6959
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 5.59901e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 24 mean_circ: 0.935575
Ran for 1050 frames Cell Events: 64 mean_circ: 0.94983
Ran for 2050 frames Cell Events: 135 mean_circ: 0.945949
Ran for 4050 frames Cell Events: 277 mean_circ: 0.950348
Ran for 5050 frames Cell Events: 367 mean_circ: 0.950348
Ran for 6050 frames Cell Events: 445 mean_circ: 0.950652
Ran for 9050 frames Cell Events: 639 mean_circ: 0.949052
Ran for 9550 frames Cell Events: 681 mean_circ: 0.948878
Ran for 10050 frames Cell Events: 732 mean_circ: 0.94947
Ran for 12050 frames Cell Events: 876 mean_circ: 0.94891
Ran for 13050 frames Cell Events: 952 mean_circ: 0.94861
Ran for 14550 frames Cell Events: 1048 mean_circ: 0.949028
Ran for 15050 frames Cell Events: 1083 mean_circ: 0.949199
Ran for 16550 frames Cell Events: 1203 mean_circ: 0.94939
Ran for 17550 frames Cell Events: 1270 mean_circ: 0.949839
Ran for 18550 frames Cell Events: 1349 mean_circ: 0.949655
Ran for 19550 frames Cell Events: 1436 mean_circ: 0.949683
Ran for 20550 frames Cell Events: 1510 mean_circ: 0.949907
Ran for 24550 frames Cell Events: 1793 mean_circ: 0.949257
Ran for 25550 frames Cell Events: 1874 mean_circ: 0.949287
Ran for 29050 frames Cell Events: 2136 mean_circ: 0.949662
Ran for 29550 frames Cell Events: 2168 mean_circ: 0.949651
Ran for 30050 frames Cell Events: 2200 mean_circ: 0.949627
Ran for 30550 frames Cell Events: 2239 mean_circ: 0.949601
Ran for 31050 frames Cell Events: 2268 mean_circ: 0.949612
Ran for 32550 frames Cell Events: 2370 mean_circ: 0.949823
Ran for 34050 frames Cell Events: 2482 mean_circ: 0.949971
Ran for 36050 frames Cell Events: 2606 mean_circ: 0.949915
Ran for 39550 frames Cell Events: 2853 mean_circ: 0.949814
Ran for 42550 frames Cell Events: 3095 mean_circ: 0.949793
Ran for 43550 frames Cell Events: 3155 mean_circ: 0.949647
Ran for 44050 frames Cell Events: 3195 mean_circ: 0.949586
Ran for 45550 frames Cell Events: 3331 mean_circ: 0.949606
Ran for 48550 frames Cell Events: 3540 mean_circ: 0.949641
Ran for 49550 frames Cell Events: 3602 mean_circ: 0.949673
Ran for 50550 frames Cell Events: 3686 mean_circ: 0.949702
Ran for 51050 frames Cell Events: 3715 mean_circ: 0.949752
Ran for 51550 frames Cell Events: 3741 mean_circ: 0.949752
Ran for 52050 frames Cell Events: 3772 mean_circ: 0.949752
Ran for 53050 frames Cell Events: 3844 mean_circ: 0.949755
Ran for 54550 frames Cell Events: 3943 mean_circ: 0.949775
Ran for 57050 frames Cell Events: 4131 mean_circ: 0.949814
Ran for 57550 frames Cell Events: 4156 mean_circ: 0.949821
Ran for 58050 frames Cell Events: 4188 mean_circ: 0.9499
Ran for 59050 frames Cell Events: 4247 mean_circ: 0.949909
Ran for 59550 frames Cell Events: 4287 mean_circ: 0.94987
Ran for 62050 frames Cell Events: 4465 mean_circ: 0.949957
Ran for 62550 frames Cell Events: 4505 mean_circ: 0.949952
Ran for 64050 frames Cell Events: 4614 mean_circ: 0.949946
Ran for 66050 frames Cell Events: 4770 mean_circ: 0.949888
Ran for 66550 frames Cell Events: 4800 mean_circ: 0.949871
Ran for 68050 frames Cell Events: 4905 mean_circ: 0.949821
Ran for 70050 frames Cell Events: 5087 mean_circ: 0.949911
Ran for 70550 frames Cell Events: 5131 mean_circ: 0.949911
Ran for 71550 frames Cell Events: 5204 mean_circ: 0.949911
Ran for 72050 frames Cell Events: 5244 mean_circ: 0.949881
Ran for 75050 frames Cell Events: 5499 mean_circ: 0.949728
Ran for 76050 frames Cell Events: 5573 mean_circ: 0.949769
Ran for 77050 frames Cell Events: 5651 mean_circ: 0.949832
Ran for 77550 frames Cell Events: 5681 mean_circ: 0.94987
Ran for 78550 frames Cell Events: 5765 mean_circ: 0.949908
Ran for 79050 frames Cell Events: 5790 mean_circ: 0.949887
Ran for 80050 frames Cell Events: 5873 mean_circ: 0.949758
Ran for 80550 frames Cell Events: 5911 mean_circ: 0.949771
Ran for 86050 frames Cell Events: 6322 mean_circ: 0.949769
Ran for 88050 frames Cell Events: 6464 mean_circ: 0.949775
Ran for 90550 frames Cell Events: 6633 mean_circ: 0.949775
Ran for 91050 frames Cell Events: 6658 mean_circ: 0.94979
Ran for 93050 frames Cell Events: 6823 mean_circ: 0.949783
Ran for 94550 frames Cell Events: 6923 mean_circ: 0.949863
Ran for 97050 frames Cell Events: 7076 mean_circ: 0.949833
Ran for 97550 frames Cell Events: 7109 mean_circ: 0.949835
Ran for 99050 frames Cell Events: 7198 mean_circ: 0.949881
Ran for 100550 frames Cell Events: 7322 mean_circ: 0.949805
Ran for 101050 frames Cell Events: 7368 mean_circ: 0.949805
Ran for 103050 frames Cell Events: 7536 mean_circ: 0.949794
Ran for 103550 frames Cell Events: 7578 mean_circ: 0.949771
Ran for 106550 frames Cell Events: 7819 mean_circ: 0.949579
Ran for 107050 frames Cell Events: 7859 mean_circ: 0.949615
Ran for 107550 frames Cell Events: 7888 mean_circ: 0.949613
Ran for 108050 frames Cell Events: 7922 mean_circ: 0.949597
Ran for 108550 frames Cell Events: 7956 mean_circ: 0.949584
Ran for 110550 frames Cell Events: 8076 mean_circ: 0.949608
Ran for 113050 frames Cell Events: 8281 mean_circ: 0.949536
Ran for 114550 frames Cell Events: 8390 mean_circ: 0.949491
Ran for 116050 frames Cell Events: 8523 mean_circ: 0.949479
Ran for 116550 frames Cell Events: 8557 mean_circ: 0.949376
Ran for 119050 frames Cell Events: 8756 mean_circ: 0.949415
Ran for 121550 frames Cell Events: 8925 mean_circ: 0.949269
Ran for 122050 frames Cell Events: 8969 mean_circ: 0.949222
Ran for 122550 frames Cell Events: 8987 mean_circ: 0.94923
Ran for 124050 frames Cell Events: 9121 mean_circ: 0.949187
Ran for 124550 frames Cell Events: 9166 mean_circ: 0.949187
Ran for 125050 frames Cell Events: 9194 mean_circ: 0.949214
Ran for 126050 frames Cell Events: 9267 mean_circ: 0.949243
Ran for 127550 frames Cell Events: 9363 mean_circ: 0.949269
Ran for 128050 frames Cell Events: 9409 mean_circ: 0.949269
Ran for 129550 frames Cell Events: 9519 mean_circ: 0.949268
Ran for 130550 frames Cell Events: 9583 mean_circ: 0.949217
Ran for 133550 frames Cell Events: 9788 mean_circ: 0.949253
Ran for 134050 frames Cell Events: 9806 mean_circ: 0.949207
Ran for 135050 frames Cell Events: 9874 mean_circ: 0.949225
Ran for 136050 frames Cell Events: 9927 mean_circ: 0.94921



End this measurment.
because stop condition (time/nr of cells) was met

Stopping time: 5.59969e+008
****************************************
Measurement finished

Mean Circularitiy: 0.94923
Mean Area: 610.77
Mean FL-1 intensity: 3772.01
Mean FL-2 intensity: 3071.87
Mean FL-3 intensity: 0

frames processed: 131641
Total Time: 68530.8ms
Number of Runs (new bg img) 0
Frames collected: 136841
Skipped frames: 17055
Rescued Frames: 1327611
Samples DAQ: 34218013
Samples written: 68420314
Samples per Channel: 34210157
FPS: 1996.78

Samples per second: 499194
Expected samples/s: 500000

Events: 10001
Events per second: 145.934
jumped back (PMT): 2966
#Frames read, frequency
# 0 frames :215802
# 1 frames :28430
# 2 frames :2134
# 3 frames :822
# 4 frames :553
# 5 frames :296
# 6 frames :1029
# 7 frames :1057
# 8 frames :645
# 9 frames :515
# 10 frames :574
# 11 frames :345
# 12 frames :303
# 13 frames :205
# 14 frames :206
# 15 frames :243
# 16 frames :206
# 17 frames :156
# 18 frames :151
# 19 frames :145
# 20 frames :108
# 21 frames :101
# 22 frames :100
# 23 frames :88
# 24 frames :94
# 25 frames :71
# 26 frames :50
# 27 frames :69
# 28 frames :52
# 29 frames :54
# 30 frames :45
# 31 frames :50
# 32 frames :34
# 33 frames :31
# 34 frames :29
# 35 frames :36
# 36 frames :25
# 37 frames :29
# 38 frames :28
# 39 frames :23
# 40 frames :24
# 41 frames :16
# 42 frames :10
# 43 frames :16
# 44 frames :15
# 45 frames :8
# 46 frames :8
# 47 frames :8
# 48 frames :5
# 49 frames :9
# 50 frames :11
# 51 frames :7
# 52 frames :4
# 53 frames :9
# 54 frames :4
# 55 frames :5
# 56 frames :3
# 58 frames :1
# 59 frames :2
# 60 frames :1
# 61 frames :3
# 62 frames :1
# 63 frames :1
# 64 frames :3
# 65 frames :3
# 66 frames :1
# 67 frames :3
# 69 frames :3
# 70 frames :1
# 75 frames :2
# 79 frames :1
# 80 frames :1
# 82 frames :1
# 84 frames :1
# 85 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.6959
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0