Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_19.39.40 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "ceaf5fc8ffdfc69f6bff3831f31a2748",
"name": "M1_20170620_1528_dsRNA_W6_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "17:40:00"
}
}
dclab-tdms2rtdc-warnings MultipleSamplesPerEventFound
in dclab.rtdc_dataset.fmt_tdms.exc line 101:
Ignoring trace data of
'\\syn03.frontend.storage.int.mpl.mpg.de\guck_division2\Members\Laura\20240219_Kasia_screen_all_folders\20170620_katarzyna_kc167_phospho_screen2\20170620_1528_dsRNA_W6_faster\M1_20170620_1528_dsRNA_W6_faster_traces.tdms'
due to multiple values for samples per event: [200 400]
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8172 (max 8174) in '8172'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.8348
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 5.62662e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 37 mean_circ: 0.894816
Ran for 1050 frames Cell Events: 65 mean_circ: 0.922134
Ran for 3050 frames Cell Events: 183 mean_circ: 0.938792
Ran for 3550 frames Cell Events: 205 mean_circ: 0.938953
Ran for 5050 frames Cell Events: 263 mean_circ: 0.939898
Ran for 6050 frames Cell Events: 302 mean_circ: 0.940988
Ran for 6550 frames Cell Events: 327 mean_circ: 0.940434
Ran for 9050 frames Cell Events: 477 mean_circ: 0.939827
Ran for 9550 frames Cell Events: 508 mean_circ: 0.939942
Ran for 10550 frames Cell Events: 565 mean_circ: 0.939709
Ran for 11050 frames Cell Events: 583 mean_circ: 0.940227
Ran for 12050 frames Cell Events: 631 mean_circ: 0.940442
Ran for 13550 frames Cell Events: 717 mean_circ: 0.940622
Ran for 14050 frames Cell Events: 748 mean_circ: 0.940752
Ran for 15550 frames Cell Events: 820 mean_circ: 0.941347
Ran for 16050 frames Cell Events: 841 mean_circ: 0.941442
Ran for 17550 frames Cell Events: 928 mean_circ: 0.94187
Ran for 18050 frames Cell Events: 950 mean_circ: 0.941972
Ran for 18550 frames Cell Events: 976 mean_circ: 0.941973
Ran for 20550 frames Cell Events: 1087 mean_circ: 0.942113
Ran for 22550 frames Cell Events: 1234 mean_circ: 0.941809
Ran for 23050 frames Cell Events: 1251 mean_circ: 0.941993
Ran for 23550 frames Cell Events: 1276 mean_circ: 0.941886
Ran for 24550 frames Cell Events: 1325 mean_circ: 0.941516
Ran for 25050 frames Cell Events: 1356 mean_circ: 0.941572
Ran for 25550 frames Cell Events: 1379 mean_circ: 0.941601
Ran for 26550 frames Cell Events: 1433 mean_circ: 0.941441
Ran for 27550 frames Cell Events: 1490 mean_circ: 0.941568
Ran for 28050 frames Cell Events: 1512 mean_circ: 0.941725
Ran for 28550 frames Cell Events: 1534 mean_circ: 0.941833
Ran for 29050 frames Cell Events: 1559 mean_circ: 0.941732
Ran for 30550 frames Cell Events: 1626 mean_circ: 0.94151
Ran for 31050 frames Cell Events: 1647 mean_circ: 0.94118
Ran for 32550 frames Cell Events: 1732 mean_circ: 0.941165
Ran for 33050 frames Cell Events: 1756 mean_circ: 0.941171
Ran for 34050 frames Cell Events: 1788 mean_circ: 0.941451
Ran for 34550 frames Cell Events: 1815 mean_circ: 0.941436
Ran for 35050 frames Cell Events: 1843 mean_circ: 0.941233
Ran for 36050 frames Cell Events: 1886 mean_circ: 0.941076
Ran for 36550 frames Cell Events: 1906 mean_circ: 0.940957
Ran for 37050 frames Cell Events: 1928 mean_circ: 0.941032
Ran for 37550 frames Cell Events: 1965 mean_circ: 0.940962
Ran for 38050 frames Cell Events: 1992 mean_circ: 0.940962
Ran for 39050 frames Cell Events: 2038 mean_circ: 0.940957
Ran for 39550 frames Cell Events: 2063 mean_circ: 0.940824
Ran for 40050 frames Cell Events: 2089 mean_circ: 0.940886
Ran for 40550 frames Cell Events: 2113 mean_circ: 0.940913
Ran for 41050 frames Cell Events: 2130 mean_circ: 0.940864
Ran for 42050 frames Cell Events: 2177 mean_circ: 0.940729
Ran for 42550 frames Cell Events: 2195 mean_circ: 0.940749
Ran for 43050 frames Cell Events: 2215 mean_circ: 0.940843
Ran for 43550 frames Cell Events: 2239 mean_circ: 0.940947
Ran for 44550 frames Cell Events: 2292 mean_circ: 0.940885
Ran for 45050 frames Cell Events: 2317 mean_circ: 0.940903
Ran for 46550 frames Cell Events: 2398 mean_circ: 0.941082
Ran for 47050 frames Cell Events: 2416 mean_circ: 0.941008
Ran for 48550 frames Cell Events: 2480 mean_circ: 0.941067
Ran for 50050 frames Cell Events: 2557 mean_circ: 0.940846
Ran for 50550 frames Cell Events: 2585 mean_circ: 0.940877
Ran for 51050 frames Cell Events: 2605 mean_circ: 0.940888
Ran for 51550 frames Cell Events: 2633 mean_circ: 0.940956
Ran for 52050 frames Cell Events: 2660 mean_circ: 0.940958
Ran for 53050 frames Cell Events: 2719 mean_circ: 0.941034
Ran for 53550 frames Cell Events: 2750 mean_circ: 0.941113
Ran for 54550 frames Cell Events: 2777 mean_circ: 0.941109
Ran for 55050 frames Cell Events: 2809 mean_circ: 0.941157
Ran for 57050 frames Cell Events: 2855 mean_circ: 0.941229
Ran for 57550 frames Cell Events: 2879 mean_circ: 0.940916
Ran for 58550 frames Cell Events: 2931 mean_circ: 0.941066
Ran for 59050 frames Cell Events: 2951 mean_circ: 0.941049
Ran for 63550 frames Cell Events: 3199 mean_circ: 0.941269
Ran for 64050 frames Cell Events: 3224 mean_circ: 0.941304
Ran for 64550 frames Cell Events: 3246 mean_circ: 0.941313
Ran for 65550 frames Cell Events: 3284 mean_circ: 0.941347
Ran for 67550 frames Cell Events: 3397 mean_circ: 0.94146
Ran for 68050 frames Cell Events: 3425 mean_circ: 0.941429
Ran for 68550 frames Cell Events: 3464 mean_circ: 0.941427
Ran for 69550 frames Cell Events: 3511 mean_circ: 0.941503
Ran for 72050 frames Cell Events: 3656 mean_circ: 0.941595
Ran for 72550 frames Cell Events: 3682 mean_circ: 0.941643
Ran for 73050 frames Cell Events: 3705 mean_circ: 0.941755
Ran for 74550 frames Cell Events: 3803 mean_circ: 0.941567
Ran for 75050 frames Cell Events: 3829 mean_circ: 0.941623
Ran for 77050 frames Cell Events: 3935 mean_circ: 0.941659
Ran for 77550 frames Cell Events: 3962 mean_circ: 0.941747
Ran for 78550 frames Cell Events: 4018 mean_circ: 0.941709
Ran for 79550 frames Cell Events: 4064 mean_circ: 0.941677
Ran for 81550 frames Cell Events: 4178 mean_circ: 0.941899
Ran for 82050 frames Cell Events: 4207 mean_circ: 0.941749
Ran for 82550 frames Cell Events: 4241 mean_circ: 0.941717
Ran for 83050 frames Cell Events: 4262 mean_circ: 0.941682
Ran for 85050 frames Cell Events: 4388 mean_circ: 0.941634
Ran for 86550 frames Cell Events: 4500 mean_circ: 0.941482
Ran for 87050 frames Cell Events: 4527 mean_circ: 0.941498
Ran for 87550 frames Cell Events: 4549 mean_circ: 0.941537
Ran for 88550 frames Cell Events: 4614 mean_circ: 0.941625
Ran for 89050 frames Cell Events: 4637 mean_circ: 0.941681
Ran for 89550 frames Cell Events: 4667 mean_circ: 0.941696
Ran for 90550 frames Cell Events: 4702 mean_circ: 0.941693
Ran for 91050 frames Cell Events: 4719 mean_circ: 0.941717
Ran for 92050 frames Cell Events: 4776 mean_circ: 0.941709
Ran for 92550 frames Cell Events: 4805 mean_circ: 0.94171
Ran for 93050 frames Cell Events: 4827 mean_circ: 0.941736
Ran for 93550 frames Cell Events: 4847 mean_circ: 0.94173
Ran for 95550 frames Cell Events: 4961 mean_circ: 0.941631
Ran for 96050 frames Cell Events: 4986 mean_circ: 0.941671
Ran for 97550 frames Cell Events: 5069 mean_circ: 0.941658
Ran for 98550 frames Cell Events: 5127 mean_circ: 0.941719
Ran for 102550 frames Cell Events: 5368 mean_circ: 0.941593
Ran for 103050 frames Cell Events: 5386 mean_circ: 0.941613
Ran for 104050 frames Cell Events: 5454 mean_circ: 0.941668
Ran for 105550 frames Cell Events: 5539 mean_circ: 0.941685
Ran for 106550 frames Cell Events: 5596 mean_circ: 0.941674
Ran for 107050 frames Cell Events: 5619 mean_circ: 0.941645
Ran for 107550 frames Cell Events: 5645 mean_circ: 0.941629
Ran for 108550 frames Cell Events: 5693 mean_circ: 0.941628
Ran for 109050 frames Cell Events: 5705 mean_circ: 0.941653
Ran for 110550 frames Cell Events: 5775 mean_circ: 0.941652
Ran for 111050 frames Cell Events: 5796 mean_circ: 0.941633
Ran for 111550 frames Cell Events: 5813 mean_circ: 0.941682
Ran for 112550 frames Cell Events: 5859 mean_circ: 0.941652
Ran for 113550 frames Cell Events: 5910 mean_circ: 0.9416
Ran for 114550 frames Cell Events: 5948 mean_circ: 0.941655
Ran for 115050 frames Cell Events: 5982 mean_circ: 0.941646
Ran for 115550 frames Cell Events: 6007 mean_circ: 0.941657
Ran for 116050 frames Cell Events: 6038 mean_circ: 0.941691
Ran for 117550 frames Cell Events: 6140 mean_circ: 0.941698
Ran for 118050 frames Cell Events: 6164 mean_circ: 0.941726
Ran for 119050 frames Cell Events: 6214 mean_circ: 0.941673
Ran for 119550 frames Cell Events: 6235 mean_circ: 0.941711
Ran for 120050 frames Cell Events: 6258 mean_circ: 0.94174
Ran for 120550 frames Cell Events: 6281 mean_circ: 0.941736
Ran for 121050 frames Cell Events: 6306 mean_circ: 0.941739
Ran for 123050 frames Cell Events: 6422 mean_circ: 0.941868
Ran for 123550 frames Cell Events: 6446 mean_circ: 0.941847
Ran for 124050 frames Cell Events: 6475 mean_circ: 0.941844
Ran for 125550 frames Cell Events: 6543 mean_circ: 0.941789
Ran for 126050 frames Cell Events: 6569 mean_circ: 0.941739
Ran for 126550 frames Cell Events: 6593 mean_circ: 0.941765
Ran for 127050 frames Cell Events: 6617 mean_circ: 0.941774
Ran for 128050 frames Cell Events: 6663 mean_circ: 0.941745
Ran for 128550 frames Cell Events: 6684 mean_circ: 0.941744
Ran for 131050 frames Cell Events: 6809 mean_circ: 0.941657
Ran for 132550 frames Cell Events: 6903 mean_circ: 0.941786
Ran for 133050 frames Cell Events: 6921 mean_circ: 0.941796
Ran for 134050 frames Cell Events: 6963 mean_circ: 0.941787
Ran for 134550 frames Cell Events: 6986 mean_circ: 0.941775
Ran for 135050 frames Cell Events: 7003 mean_circ: 0.94181
Ran for 135550 frames Cell Events: 7030 mean_circ: 0.941787
Ran for 136050 frames Cell Events: 7055 mean_circ: 0.941844
Ran for 136550 frames Cell Events: 7073 mean_circ: 0.941855
Ran for 137050 frames Cell Events: 7097 mean_circ: 0.941855
Ran for 138050 frames Cell Events: 7158 mean_circ: 0.941809
Ran for 138550 frames Cell Events: 7181 mean_circ: 0.941821
Ran for 139050 frames Cell Events: 7213 mean_circ: 0.941721
Ran for 140050 frames Cell Events: 7276 mean_circ: 0.941754
Ran for 140550 frames Cell Events: 7304 mean_circ: 0.94178
Ran for 141550 frames Cell Events: 7347 mean_circ: 0.941687
Ran for 143050 frames Cell Events: 7419 mean_circ: 0.941645
Ran for 144050 frames Cell Events: 7479 mean_circ: 0.941668
Ran for 144550 frames Cell Events: 7503 mean_circ: 0.941664
Ran for 145050 frames Cell Events: 7533 mean_circ: 0.941698
Ran for 145550 frames Cell Events: 7562 mean_circ: 0.941662
Ran for 147050 frames Cell Events: 7656 mean_circ: 0.941629
Ran for 148550 frames Cell Events: 7736 mean_circ: 0.941619
Ran for 149050 frames Cell Events: 7760 mean_circ: 0.941636
Ran for 151050 frames Cell Events: 7853 mean_circ: 0.941551
Ran for 151550 frames Cell Events: 7878 mean_circ: 0.941597
Ran for 152550 frames Cell Events: 7930 mean_circ: 0.94162
Ran for 153550 frames Cell Events: 7991 mean_circ: 0.941629
Ran for 155550 frames Cell Events: 8073 mean_circ: 0.941579
Ran for 157050 frames Cell Events: 8149 mean_circ: 0.941567
Ran for 157550 frames Cell Events: 8158 mean_circ: 0.941575



End this measurment..



Stopping time: 5.62741e+008
****************************************
Measurement finished

Mean Circularitiy: 0.941576
Mean Area: 591.926
Mean FL-1 intensity: 3495.64
Mean FL-2 intensity: 4232.65
Mean FL-3 intensity: 0

frames processed: 155146
Total Time: 78970.9ms
Number of Runs (new bg img) 0
Frames collected: 157813
Skipped frames: 37384
Rescued Frames: 1940075
Samples DAQ: 39455069
Samples written: 78906508
Samples per Channel: 39453254
FPS: 1998.37

Samples per second: 499593
Expected samples/s: 500000

Events: 8175
Events per second: 103.519
jumped back (PMT): 22
#Frames read, frequency
# 0 frames :235370
# 1 frames :65227
# 2 frames :4069
# 3 frames :1013
# 4 frames :679
# 5 frames :284
# 6 frames :1951
# 7 frames :2037
# 8 frames :1108
# 9 frames :694
# 10 frames :414
# 11 frames :297
# 12 frames :235
# 13 frames :234
# 14 frames :202
# 15 frames :181
# 16 frames :135
# 17 frames :100
# 18 frames :63
# 19 frames :67
# 20 frames :42
# 21 frames :51
# 22 frames :40
# 23 frames :37
# 24 frames :21
# 25 frames :34
# 26 frames :19
# 27 frames :22
# 28 frames :9
# 29 frames :10
# 30 frames :9
# 31 frames :5
# 32 frames :7
# 33 frames :2
# 34 frames :6
# 36 frames :1
# 37 frames :2
# 38 frames :2
# 39 frames :4
# 40 frames :2
# 41 frames :1
# 42 frames :1
# 43 frames :3
# 44 frames :3
# 45 frames :1
# 46 frames :1
# 50 frames :1
# 54 frames :1
# 58 frames :1
# 62 frames :1
# 79 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.8348
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0