Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_19.44.01 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "b9dbf9c206fdc8fb635f1a3c099fe36d",
"name": "M1_20170620_1630_dsRNA_K6_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "17:44:25"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9434 (max 9435) in '9434'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.992
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 5.66386e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 3050 frames Cell Events: 135 mean_circ: 0.95099
Ran for 3550 frames Cell Events: 150 mean_circ: 0.951554
Ran for 4050 frames Cell Events: 168 mean_circ: 0.950112
Ran for 4550 frames Cell Events: 188 mean_circ: 0.950104
Ran for 5050 frames Cell Events: 200 mean_circ: 0.950241
Ran for 6550 frames Cell Events: 263 mean_circ: 0.949628
Ran for 7550 frames Cell Events: 308 mean_circ: 0.949481
Ran for 8550 frames Cell Events: 359 mean_circ: 0.950156
Ran for 9050 frames Cell Events: 380 mean_circ: 0.949727
Ran for 9550 frames Cell Events: 398 mean_circ: 0.950115
Ran for 10050 frames Cell Events: 424 mean_circ: 0.949572
Ran for 10550 frames Cell Events: 450 mean_circ: 0.949362
Ran for 13050 frames Cell Events: 580 mean_circ: 0.950355
Ran for 13550 frames Cell Events: 614 mean_circ: 0.950105
Ran for 14050 frames Cell Events: 629 mean_circ: 0.950344
Ran for 15050 frames Cell Events: 679 mean_circ: 0.950357
Ran for 15550 frames Cell Events: 700 mean_circ: 0.95032
Ran for 16050 frames Cell Events: 715 mean_circ: 0.950347
Ran for 16550 frames Cell Events: 733 mean_circ: 0.950209
Ran for 17550 frames Cell Events: 775 mean_circ: 0.9509
Ran for 18050 frames Cell Events: 791 mean_circ: 0.951055
Ran for 18550 frames Cell Events: 820 mean_circ: 0.951029
Ran for 19550 frames Cell Events: 883 mean_circ: 0.950812
Ran for 20550 frames Cell Events: 925 mean_circ: 0.950875
Ran for 21050 frames Cell Events: 963 mean_circ: 0.950852
Ran for 22050 frames Cell Events: 1001 mean_circ: 0.950796
Ran for 22550 frames Cell Events: 1029 mean_circ: 0.950916
Ran for 23550 frames Cell Events: 1080 mean_circ: 0.95132
Ran for 24550 frames Cell Events: 1131 mean_circ: 0.951468
Ran for 26550 frames Cell Events: 1211 mean_circ: 0.951609
Ran for 27550 frames Cell Events: 1272 mean_circ: 0.951833
Ran for 29050 frames Cell Events: 1343 mean_circ: 0.951745
Ran for 29550 frames Cell Events: 1363 mean_circ: 0.951746
Ran for 30050 frames Cell Events: 1391 mean_circ: 0.951728
Ran for 32050 frames Cell Events: 1508 mean_circ: 0.951341
Ran for 35050 frames Cell Events: 1692 mean_circ: 0.951339
Ran for 36550 frames Cell Events: 1762 mean_circ: 0.951296
Ran for 37550 frames Cell Events: 1805 mean_circ: 0.951428
Ran for 39050 frames Cell Events: 1876 mean_circ: 0.951146
Ran for 39550 frames Cell Events: 1906 mean_circ: 0.951149
Ran for 41550 frames Cell Events: 2008 mean_circ: 0.951061
Ran for 42050 frames Cell Events: 2034 mean_circ: 0.951017
Ran for 43050 frames Cell Events: 2091 mean_circ: 0.950785
Ran for 44050 frames Cell Events: 2124 mean_circ: 0.950623
Ran for 44550 frames Cell Events: 2145 mean_circ: 0.95063
Ran for 46050 frames Cell Events: 2250 mean_circ: 0.950422
Ran for 46550 frames Cell Events: 2290 mean_circ: 0.950535
Ran for 47050 frames Cell Events: 2324 mean_circ: 0.950358
Ran for 48050 frames Cell Events: 2361 mean_circ: 0.95032
Ran for 50550 frames Cell Events: 2509 mean_circ: 0.950527
Ran for 51050 frames Cell Events: 2533 mean_circ: 0.95059
Ran for 51550 frames Cell Events: 2560 mean_circ: 0.950576
Ran for 52550 frames Cell Events: 2613 mean_circ: 0.950627
Ran for 53550 frames Cell Events: 2672 mean_circ: 0.950582
Ran for 54050 frames Cell Events: 2703 mean_circ: 0.950589
Ran for 55550 frames Cell Events: 2790 mean_circ: 0.950788
Ran for 57050 frames Cell Events: 2858 mean_circ: 0.950674
Ran for 58550 frames Cell Events: 2937 mean_circ: 0.950643
Ran for 59050 frames Cell Events: 2956 mean_circ: 0.950599
Ran for 59550 frames Cell Events: 2977 mean_circ: 0.950513
Ran for 60550 frames Cell Events: 3032 mean_circ: 0.950477
Ran for 61050 frames Cell Events: 3046 mean_circ: 0.950469
Ran for 61550 frames Cell Events: 3077 mean_circ: 0.950419
Ran for 62050 frames Cell Events: 3085 mean_circ: 0.950426
Ran for 62550 frames Cell Events: 3106 mean_circ: 0.950449
Ran for 63050 frames Cell Events: 3123 mean_circ: 0.95039
Ran for 64050 frames Cell Events: 3163 mean_circ: 0.950399
Ran for 65550 frames Cell Events: 3257 mean_circ: 0.950268
Ran for 66050 frames Cell Events: 3284 mean_circ: 0.950255
Ran for 66550 frames Cell Events: 3306 mean_circ: 0.95031
Ran for 67050 frames Cell Events: 3324 mean_circ: 0.950278
Ran for 67550 frames Cell Events: 3352 mean_circ: 0.950246
Ran for 68050 frames Cell Events: 3362 mean_circ: 0.95024
Ran for 69050 frames Cell Events: 3415 mean_circ: 0.95037
Ran for 69550 frames Cell Events: 3445 mean_circ: 0.950368
Ran for 70050 frames Cell Events: 3479 mean_circ: 0.950355
Ran for 71050 frames Cell Events: 3519 mean_circ: 0.950183
Ran for 71550 frames Cell Events: 3548 mean_circ: 0.950243
Ran for 72050 frames Cell Events: 3571 mean_circ: 0.950254
Ran for 72550 frames Cell Events: 3595 mean_circ: 0.950262
Ran for 73050 frames Cell Events: 3616 mean_circ: 0.950303
Ran for 74550 frames Cell Events: 3696 mean_circ: 0.950276
Ran for 75550 frames Cell Events: 3752 mean_circ: 0.950249
Ran for 76550 frames Cell Events: 3790 mean_circ: 0.950242
Ran for 77050 frames Cell Events: 3824 mean_circ: 0.95019
Ran for 78050 frames Cell Events: 3862 mean_circ: 0.95015
Ran for 78550 frames Cell Events: 3898 mean_circ: 0.950131
Ran for 79050 frames Cell Events: 3907 mean_circ: 0.950118
Ran for 79550 frames Cell Events: 3928 mean_circ: 0.950112
Ran for 80050 frames Cell Events: 3938 mean_circ: 0.950073
Ran for 81550 frames Cell Events: 3994 mean_circ: 0.950051
Ran for 82050 frames Cell Events: 4012 mean_circ: 0.950049
Ran for 84050 frames Cell Events: 4127 mean_circ: 0.950041
Ran for 84550 frames Cell Events: 4153 mean_circ: 0.950029
Ran for 85050 frames Cell Events: 4184 mean_circ: 0.950051
Ran for 85550 frames Cell Events: 4205 mean_circ: 0.950075
Ran for 86050 frames Cell Events: 4223 mean_circ: 0.950083
Ran for 88550 frames Cell Events: 4326 mean_circ: 0.949925
Ran for 89050 frames Cell Events: 4335 mean_circ: 0.949821
Ran for 90050 frames Cell Events: 4377 mean_circ: 0.94975
Ran for 90550 frames Cell Events: 4397 mean_circ: 0.949767
Ran for 92550 frames Cell Events: 4496 mean_circ: 0.949691
Ran for 93050 frames Cell Events: 4512 mean_circ: 0.949697
Ran for 94050 frames Cell Events: 4565 mean_circ: 0.949732
Ran for 94550 frames Cell Events: 4590 mean_circ: 0.949712
Ran for 95550 frames Cell Events: 4642 mean_circ: 0.94971
Ran for 97050 frames Cell Events: 4719 mean_circ: 0.949693
Ran for 97550 frames Cell Events: 4742 mean_circ: 0.949544
Ran for 100550 frames Cell Events: 4926 mean_circ: 0.949539
Ran for 101050 frames Cell Events: 4953 mean_circ: 0.949557
Ran for 102050 frames Cell Events: 5011 mean_circ: 0.949526
Ran for 102550 frames Cell Events: 5026 mean_circ: 0.949524
Ran for 103050 frames Cell Events: 5051 mean_circ: 0.949539
Ran for 104050 frames Cell Events: 5095 mean_circ: 0.949582
Ran for 108050 frames Cell Events: 5351 mean_circ: 0.949588
Ran for 108550 frames Cell Events: 5369 mean_circ: 0.949634
Ran for 109050 frames Cell Events: 5410 mean_circ: 0.949555
Ran for 109550 frames Cell Events: 5439 mean_circ: 0.949566
Ran for 110050 frames Cell Events: 5468 mean_circ: 0.949601
Ran for 111050 frames Cell Events: 5511 mean_circ: 0.949598
Ran for 111550 frames Cell Events: 5537 mean_circ: 0.949602
Ran for 113050 frames Cell Events: 5635 mean_circ: 0.949601
Ran for 113550 frames Cell Events: 5665 mean_circ: 0.949596
Ran for 115050 frames Cell Events: 5755 mean_circ: 0.949583
Ran for 116550 frames Cell Events: 5850 mean_circ: 0.949608
Ran for 117050 frames Cell Events: 5879 mean_circ: 0.949596
Ran for 119550 frames Cell Events: 6052 mean_circ: 0.949631
Ran for 121050 frames Cell Events: 6133 mean_circ: 0.949676
Ran for 122050 frames Cell Events: 6192 mean_circ: 0.949713
Ran for 123550 frames Cell Events: 6266 mean_circ: 0.949724
Ran for 124050 frames Cell Events: 6298 mean_circ: 0.949739
Ran for 126550 frames Cell Events: 6423 mean_circ: 0.94971
Ran for 128050 frames Cell Events: 6498 mean_circ: 0.949658
Ran for 129550 frames Cell Events: 6580 mean_circ: 0.949693
Ran for 130050 frames Cell Events: 6606 mean_circ: 0.949669
Ran for 130550 frames Cell Events: 6638 mean_circ: 0.949667
Ran for 131050 frames Cell Events: 6654 mean_circ: 0.949645
Ran for 131550 frames Cell Events: 6689 mean_circ: 0.949676
Ran for 132550 frames Cell Events: 6749 mean_circ: 0.94963
Ran for 134050 frames Cell Events: 6851 mean_circ: 0.949655
Ran for 134550 frames Cell Events: 6882 mean_circ: 0.94967
Ran for 136050 frames Cell Events: 6983 mean_circ: 0.949709
Ran for 137050 frames Cell Events: 7037 mean_circ: 0.949736
Ran for 138050 frames Cell Events: 7117 mean_circ: 0.949717
Ran for 139050 frames Cell Events: 7182 mean_circ: 0.949727
Ran for 139550 frames Cell Events: 7209 mean_circ: 0.94974
Ran for 141050 frames Cell Events: 7310 mean_circ: 0.949748
Ran for 141550 frames Cell Events: 7349 mean_circ: 0.949694
Ran for 142550 frames Cell Events: 7411 mean_circ: 0.949685
Ran for 143050 frames Cell Events: 7428 mean_circ: 0.949709
Ran for 144050 frames Cell Events: 7484 mean_circ: 0.949693
Ran for 147050 frames Cell Events: 7687 mean_circ: 0.949525
Ran for 148050 frames Cell Events: 7760 mean_circ: 0.94955
Ran for 149550 frames Cell Events: 7864 mean_circ: 0.949644
Ran for 151550 frames Cell Events: 7960 mean_circ: 0.94969
Ran for 152050 frames Cell Events: 7991 mean_circ: 0.94971
Ran for 153050 frames Cell Events: 8052 mean_circ: 0.949701
Ran for 153550 frames Cell Events: 8087 mean_circ: 0.949663
Ran for 154550 frames Cell Events: 8152 mean_circ: 0.949654
Ran for 159050 frames Cell Events: 8446 mean_circ: 0.949574
Ran for 159550 frames Cell Events: 8470 mean_circ: 0.949607
Ran for 160550 frames Cell Events: 8533 mean_circ: 0.949507
Ran for 161050 frames Cell Events: 8561 mean_circ: 0.949508
Ran for 161550 frames Cell Events: 8585 mean_circ: 0.949511
Ran for 162050 frames Cell Events: 8614 mean_circ: 0.949519
Ran for 162550 frames Cell Events: 8638 mean_circ: 0.949485
Ran for 163550 frames Cell Events: 8678 mean_circ: 0.94952
Ran for 165050 frames Cell Events: 8759 mean_circ: 0.9495
Ran for 166050 frames Cell Events: 8830 mean_circ: 0.949504
Ran for 167550 frames Cell Events: 8908 mean_circ: 0.949529
Ran for 168550 frames Cell Events: 8969 mean_circ: 0.949485
Ran for 169550 frames Cell Events: 9032 mean_circ: 0.949465
Ran for 170050 frames Cell Events: 9054 mean_circ: 0.94949
Ran for 170550 frames Cell Events: 9076 mean_circ: 0.949512
Ran for 173550 frames Cell Events: 9267 mean_circ: 0.949557



End this measurment..



Stopping time: 5.66474e+008
****************************************
Measurement finished

Mean Circularitiy: 0.949566
Mean Area: 652.76
Mean FL-1 intensity: 4028.75
Mean FL-2 intensity: 3502.03
Mean FL-3 intensity: 0

frames processed: 174324
Total Time: 88243ms
Number of Runs (new bg img) 0
Frames collected: 176318
Skipped frames: 49953
Rescued Frames: 2785571
Samples DAQ: 44079388
Samples written: 88158722
Samples per Channel: 44079361
FPS: 1998.1

Samples per second: 499522
Expected samples/s: 500000

Events: 9435
Events per second: 106.921
jumped back (PMT): 5084
#Frames read, frequency
# 0 frames :270354
# 1 frames :66101
# 2 frames :4659
# 3 frames :1230
# 4 frames :872
# 5 frames :381
# 6 frames :1537
# 7 frames :2090
# 8 frames :1289
# 9 frames :822
# 10 frames :682
# 11 frames :345
# 12 frames :277
# 13 frames :236
# 14 frames :217
# 15 frames :257
# 16 frames :198
# 17 frames :142
# 18 frames :129
# 19 frames :78
# 20 frames :60
# 21 frames :82
# 22 frames :55
# 23 frames :60
# 24 frames :60
# 25 frames :35
# 26 frames :30
# 27 frames :31
# 28 frames :23
# 29 frames :18
# 30 frames :14
# 31 frames :8
# 32 frames :18
# 33 frames :12
# 34 frames :11
# 35 frames :11
# 36 frames :9
# 37 frames :6
# 38 frames :6
# 39 frames :6
# 40 frames :4
# 41 frames :1
# 42 frames :1
# 43 frames :5
# 44 frames :5
# 45 frames :3
# 47 frames :1
# 48 frames :1
# 49 frames :1
# 51 frames :2
# 54 frames :2
# 55 frames :1
# 56 frames :1
# 57 frames :2
# 61 frames :1
# 62 frames :2
# 63 frames :1
# 67 frames :1
# 71 frames :1
# 72 frames :1
# 88 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.992
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0