Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_19.44.33 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "fb000c2d7385009e785c87cffdb78e97",
"name": "M1_20170620_1633_dsRNA_K6_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "17:44:57"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9528 (max 9529) in '9528'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0034
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 5.6658e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 48 mean_circ: 0.936202
Ran for 1550 frames Cell Events: 75 mean_circ: 0.933389
Ran for 2050 frames Cell Events: 106 mean_circ: 0.938436
Ran for 2550 frames Cell Events: 140 mean_circ: 0.939674
Ran for 5050 frames Cell Events: 286 mean_circ: 0.938121
Ran for 6050 frames Cell Events: 356 mean_circ: 0.938795
Ran for 6550 frames Cell Events: 391 mean_circ: 0.940315
Ran for 8550 frames Cell Events: 517 mean_circ: 0.940196
Ran for 9050 frames Cell Events: 544 mean_circ: 0.940135
Ran for 9550 frames Cell Events: 573 mean_circ: 0.940296
Ran for 10050 frames Cell Events: 597 mean_circ: 0.939899
Ran for 10550 frames Cell Events: 629 mean_circ: 0.939699
Ran for 11050 frames Cell Events: 660 mean_circ: 0.94032
Ran for 11550 frames Cell Events: 687 mean_circ: 0.940307
Ran for 12050 frames Cell Events: 716 mean_circ: 0.940587
Ran for 12550 frames Cell Events: 746 mean_circ: 0.940631
Ran for 15050 frames Cell Events: 887 mean_circ: 0.940208
Ran for 15550 frames Cell Events: 924 mean_circ: 0.940277
Ran for 16550 frames Cell Events: 961 mean_circ: 0.940118
Ran for 17050 frames Cell Events: 995 mean_circ: 0.940118
Ran for 18050 frames Cell Events: 1046 mean_circ: 0.939939
Ran for 19550 frames Cell Events: 1131 mean_circ: 0.939998
Ran for 20550 frames Cell Events: 1182 mean_circ: 0.939786
Ran for 21050 frames Cell Events: 1201 mean_circ: 0.939392
Ran for 25550 frames Cell Events: 1543 mean_circ: 0.939738
Ran for 26550 frames Cell Events: 1593 mean_circ: 0.939678
Ran for 27050 frames Cell Events: 1627 mean_circ: 0.939752
Ran for 28550 frames Cell Events: 1719 mean_circ: 0.93975
Ran for 30550 frames Cell Events: 1818 mean_circ: 0.939957
Ran for 32550 frames Cell Events: 1944 mean_circ: 0.939996
Ran for 33050 frames Cell Events: 1979 mean_circ: 0.939906
Ran for 35050 frames Cell Events: 2114 mean_circ: 0.940002
Ran for 35550 frames Cell Events: 2136 mean_circ: 0.939986
Ran for 36550 frames Cell Events: 2199 mean_circ: 0.939913
Ran for 37550 frames Cell Events: 2270 mean_circ: 0.939712
Ran for 38550 frames Cell Events: 2342 mean_circ: 0.939697
Ran for 41050 frames Cell Events: 2526 mean_circ: 0.939558
Ran for 41550 frames Cell Events: 2545 mean_circ: 0.939507
Ran for 42550 frames Cell Events: 2616 mean_circ: 0.939542
Ran for 43050 frames Cell Events: 2643 mean_circ: 0.939557
Ran for 46550 frames Cell Events: 2853 mean_circ: 0.939625
Ran for 48050 frames Cell Events: 2935 mean_circ: 0.939825
Ran for 48550 frames Cell Events: 2962 mean_circ: 0.939786
Ran for 49050 frames Cell Events: 2998 mean_circ: 0.939842
Ran for 49550 frames Cell Events: 3022 mean_circ: 0.939888
Ran for 52550 frames Cell Events: 3208 mean_circ: 0.940126
Ran for 54050 frames Cell Events: 3294 mean_circ: 0.940125
Ran for 56050 frames Cell Events: 3425 mean_circ: 0.940207
Ran for 56550 frames Cell Events: 3448 mean_circ: 0.940223
Ran for 57050 frames Cell Events: 3470 mean_circ: 0.940274
Ran for 59050 frames Cell Events: 3600 mean_circ: 0.940441
Ran for 59550 frames Cell Events: 3643 mean_circ: 0.940441
Ran for 60050 frames Cell Events: 3669 mean_circ: 0.940237
Ran for 60550 frames Cell Events: 3688 mean_circ: 0.940206
Ran for 61050 frames Cell Events: 3716 mean_circ: 0.940233
Ran for 62050 frames Cell Events: 3777 mean_circ: 0.940095
Ran for 63550 frames Cell Events: 3893 mean_circ: 0.940138
Ran for 64550 frames Cell Events: 3956 mean_circ: 0.940138
Ran for 67050 frames Cell Events: 4078 mean_circ: 0.940229
Ran for 67550 frames Cell Events: 4111 mean_circ: 0.940207
Ran for 68050 frames Cell Events: 4145 mean_circ: 0.940226
Ran for 68550 frames Cell Events: 4186 mean_circ: 0.940248
Ran for 69050 frames Cell Events: 4211 mean_circ: 0.940215
Ran for 70050 frames Cell Events: 4289 mean_circ: 0.940246
Ran for 71550 frames Cell Events: 4393 mean_circ: 0.940261
Ran for 72550 frames Cell Events: 4465 mean_circ: 0.940166
Ran for 73550 frames Cell Events: 4517 mean_circ: 0.940169
Ran for 74550 frames Cell Events: 4584 mean_circ: 0.940214
Ran for 75550 frames Cell Events: 4657 mean_circ: 0.940058
Ran for 77550 frames Cell Events: 4779 mean_circ: 0.939985
Ran for 78550 frames Cell Events: 4846 mean_circ: 0.939989
Ran for 79550 frames Cell Events: 4896 mean_circ: 0.940042
Ran for 81050 frames Cell Events: 5002 mean_circ: 0.939836
Ran for 82050 frames Cell Events: 5081 mean_circ: 0.939717
Ran for 82550 frames Cell Events: 5121 mean_circ: 0.939735
Ran for 83550 frames Cell Events: 5178 mean_circ: 0.939728
Ran for 84050 frames Cell Events: 5208 mean_circ: 0.939718
Ran for 85050 frames Cell Events: 5256 mean_circ: 0.939738
Ran for 86550 frames Cell Events: 5337 mean_circ: 0.939645
Ran for 87050 frames Cell Events: 5361 mean_circ: 0.939629
Ran for 87550 frames Cell Events: 5400 mean_circ: 0.939629
Ran for 88050 frames Cell Events: 5438 mean_circ: 0.939547
Ran for 89050 frames Cell Events: 5479 mean_circ: 0.939526
Ran for 89550 frames Cell Events: 5506 mean_circ: 0.939554
Ran for 90050 frames Cell Events: 5533 mean_circ: 0.93953
Ran for 90550 frames Cell Events: 5555 mean_circ: 0.939483
Ran for 91050 frames Cell Events: 5592 mean_circ: 0.939483
Ran for 91550 frames Cell Events: 5601 mean_circ: 0.939543
Ran for 92050 frames Cell Events: 5636 mean_circ: 0.939587
Ran for 93050 frames Cell Events: 5699 mean_circ: 0.939573
Ran for 95050 frames Cell Events: 5829 mean_circ: 0.93953
Ran for 96050 frames Cell Events: 5890 mean_circ: 0.939586
Ran for 96550 frames Cell Events: 5913 mean_circ: 0.939633
Ran for 97550 frames Cell Events: 5977 mean_circ: 0.939524
Ran for 101050 frames Cell Events: 6205 mean_circ: 0.939526
Ran for 102550 frames Cell Events: 6311 mean_circ: 0.939517
Ran for 104050 frames Cell Events: 6397 mean_circ: 0.93944
Ran for 105550 frames Cell Events: 6495 mean_circ: 0.939417
Ran for 106550 frames Cell Events: 6555 mean_circ: 0.939383
Ran for 107050 frames Cell Events: 6579 mean_circ: 0.939421
Ran for 108050 frames Cell Events: 6644 mean_circ: 0.939396
Ran for 108550 frames Cell Events: 6660 mean_circ: 0.939376
Ran for 110050 frames Cell Events: 6746 mean_circ: 0.93948
Ran for 111050 frames Cell Events: 6808 mean_circ: 0.939479
Ran for 111550 frames Cell Events: 6838 mean_circ: 0.939468
Ran for 112050 frames Cell Events: 6876 mean_circ: 0.939486
Ran for 113050 frames Cell Events: 6953 mean_circ: 0.939486
Ran for 114050 frames Cell Events: 7016 mean_circ: 0.939519
Ran for 115550 frames Cell Events: 7109 mean_circ: 0.93956
Ran for 116050 frames Cell Events: 7136 mean_circ: 0.939568
Ran for 116550 frames Cell Events: 7161 mean_circ: 0.939577
Ran for 118550 frames Cell Events: 7260 mean_circ: 0.939566
Ran for 119050 frames Cell Events: 7281 mean_circ: 0.939557
Ran for 119550 frames Cell Events: 7299 mean_circ: 0.939602
Ran for 121050 frames Cell Events: 7408 mean_circ: 0.939442
Ran for 121550 frames Cell Events: 7440 mean_circ: 0.93948
Ran for 122050 frames Cell Events: 7471 mean_circ: 0.939429
Ran for 123050 frames Cell Events: 7534 mean_circ: 0.939448
Ran for 123550 frames Cell Events: 7561 mean_circ: 0.939457
Ran for 124550 frames Cell Events: 7609 mean_circ: 0.939416
Ran for 125050 frames Cell Events: 7626 mean_circ: 0.939429
Ran for 125550 frames Cell Events: 7652 mean_circ: 0.939369
Ran for 126050 frames Cell Events: 7682 mean_circ: 0.939381
Ran for 127050 frames Cell Events: 7738 mean_circ: 0.939403
Ran for 128550 frames Cell Events: 7835 mean_circ: 0.939454
Ran for 129050 frames Cell Events: 7861 mean_circ: 0.93941
Ran for 130050 frames Cell Events: 7901 mean_circ: 0.939419
Ran for 130550 frames Cell Events: 7931 mean_circ: 0.939411
Ran for 131050 frames Cell Events: 7956 mean_circ: 0.939408
Ran for 132550 frames Cell Events: 8065 mean_circ: 0.939438
Ran for 133550 frames Cell Events: 8130 mean_circ: 0.939422
Ran for 134550 frames Cell Events: 8198 mean_circ: 0.939434
Ran for 137050 frames Cell Events: 8353 mean_circ: 0.939518
Ran for 140050 frames Cell Events: 8568 mean_circ: 0.93951
Ran for 140550 frames Cell Events: 8595 mean_circ: 0.939525
Ran for 142550 frames Cell Events: 8723 mean_circ: 0.939437
Ran for 144550 frames Cell Events: 8876 mean_circ: 0.939445
Ran for 145050 frames Cell Events: 8907 mean_circ: 0.939443
Ran for 146050 frames Cell Events: 8968 mean_circ: 0.939451
Ran for 146550 frames Cell Events: 9001 mean_circ: 0.939479
Ran for 147050 frames Cell Events: 9022 mean_circ: 0.939477
Ran for 148050 frames Cell Events: 9082 mean_circ: 0.9395
Ran for 149050 frames Cell Events: 9126 mean_circ: 0.939519
Ran for 149550 frames Cell Events: 9141 mean_circ: 0.939539
Ran for 150050 frames Cell Events: 9171 mean_circ: 0.939554
Ran for 150550 frames Cell Events: 9202 mean_circ: 0.93957
Ran for 151050 frames Cell Events: 9229 mean_circ: 0.939528
Ran for 153550 frames Cell Events: 9417 mean_circ: 0.939478
Ran for 155050 frames Cell Events: 9486 mean_circ: 0.939545
Ran for 155550 frames Cell Events: 9518 mean_circ: 0.939535



End this measurment..



Stopping time: 5.66658e+008
****************************************
Measurement finished

Mean Circularitiy: 0.939541
Mean Area: 646.827
Mean FL-1 intensity: 3882.24
Mean FL-2 intensity: 3741.75
Mean FL-3 intensity: 0

frames processed: 154033
Total Time: 78008.6ms
Number of Runs (new bg img) 0
Frames collected: 155853
Skipped frames: 51773
Rescued Frames: 2874367
Samples DAQ: 38963171
Samples written: 77926280
Samples per Channel: 38963140
FPS: 1997.9

Samples per second: 499473
Expected samples/s: 500000

Events: 9529
Events per second: 122.153
jumped back (PMT): 21
#Frames read, frequency
# 0 frames :246408
# 1 frames :50786
# 2 frames :3683
# 3 frames :1079
# 4 frames :812
# 5 frames :334
# 6 frames :1628
# 7 frames :1825
# 8 frames :1232
# 9 frames :808
# 10 frames :565
# 11 frames :353
# 12 frames :309
# 13 frames :195
# 14 frames :198
# 15 frames :265
# 16 frames :211
# 17 frames :172
# 18 frames :115
# 19 frames :64
# 20 frames :82
# 21 frames :64
# 22 frames :61
# 23 frames :50
# 24 frames :51
# 25 frames :50
# 26 frames :28
# 27 frames :18
# 28 frames :23
# 29 frames :22
# 30 frames :18
# 31 frames :19
# 32 frames :20
# 33 frames :13
# 34 frames :13
# 35 frames :15
# 36 frames :8
# 37 frames :4
# 38 frames :6
# 39 frames :9
# 40 frames :1
# 41 frames :1
# 42 frames :1
# 43 frames :6
# 44 frames :2
# 45 frames :3
# 46 frames :4
# 47 frames :3
# 48 frames :3
# 49 frames :3
# 50 frames :2
# 51 frames :2
# 52 frames :2
# 53 frames :1
# 54 frames :2
# 56 frames :1
# 57 frames :1
# 61 frames :1
# 62 frames :2
# 67 frames :1
# 68 frames :1
# 77 frames :1
# 88 frames :2
# 99 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0034
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0