Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_19.48.30 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "3ef3e7b8490897fb11888279671e2d14",
"name": "M1_20170620_1728_dsRNA_W4_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "17:48:50"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8209 (max 8210) in '8209'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.5294
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 5.69825e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 2050 frames Cell Events: 99 mean_circ: 0.940565
Ran for 3050 frames Cell Events: 160 mean_circ: 0.937388
Ran for 3550 frames Cell Events: 186 mean_circ: 0.938765
Ran for 4550 frames Cell Events: 245 mean_circ: 0.937241
Ran for 5050 frames Cell Events: 260 mean_circ: 0.937056
Ran for 5550 frames Cell Events: 292 mean_circ: 0.937013
Ran for 6050 frames Cell Events: 312 mean_circ: 0.937785
Ran for 6550 frames Cell Events: 333 mean_circ: 0.937591
Ran for 7050 frames Cell Events: 355 mean_circ: 0.93791
Ran for 8050 frames Cell Events: 417 mean_circ: 0.93832
Ran for 8550 frames Cell Events: 447 mean_circ: 0.937695
Ran for 9050 frames Cell Events: 463 mean_circ: 0.937772
Ran for 9550 frames Cell Events: 488 mean_circ: 0.938003
Ran for 11050 frames Cell Events: 565 mean_circ: 0.937988
Ran for 12050 frames Cell Events: 631 mean_circ: 0.936967
Ran for 12550 frames Cell Events: 654 mean_circ: 0.937304
Ran for 13050 frames Cell Events: 682 mean_circ: 0.937067
Ran for 13550 frames Cell Events: 708 mean_circ: 0.937134
Ran for 15550 frames Cell Events: 805 mean_circ: 0.938171
Ran for 16050 frames Cell Events: 834 mean_circ: 0.93796
Ran for 16550 frames Cell Events: 865 mean_circ: 0.938087
Ran for 17050 frames Cell Events: 896 mean_circ: 0.937956
Ran for 17550 frames Cell Events: 919 mean_circ: 0.938096
Ran for 18050 frames Cell Events: 945 mean_circ: 0.937644
Ran for 18550 frames Cell Events: 980 mean_circ: 0.937571
Ran for 19550 frames Cell Events: 1037 mean_circ: 0.937028
Ran for 20050 frames Cell Events: 1067 mean_circ: 0.936862
Ran for 20550 frames Cell Events: 1106 mean_circ: 0.937149
Ran for 21050 frames Cell Events: 1129 mean_circ: 0.937202
Ran for 21550 frames Cell Events: 1161 mean_circ: 0.937406
Ran for 23050 frames Cell Events: 1260 mean_circ: 0.937326
Ran for 23550 frames Cell Events: 1284 mean_circ: 0.937124
Ran for 24050 frames Cell Events: 1301 mean_circ: 0.937132
Ran for 24550 frames Cell Events: 1320 mean_circ: 0.937069
Ran for 25050 frames Cell Events: 1347 mean_circ: 0.937173
Ran for 25550 frames Cell Events: 1369 mean_circ: 0.937231
Ran for 26050 frames Cell Events: 1397 mean_circ: 0.93727
Ran for 26550 frames Cell Events: 1419 mean_circ: 0.93724
Ran for 28050 frames Cell Events: 1500 mean_circ: 0.93701
Ran for 29050 frames Cell Events: 1558 mean_circ: 0.936908
Ran for 29550 frames Cell Events: 1594 mean_circ: 0.936919
Ran for 30050 frames Cell Events: 1613 mean_circ: 0.936651
Ran for 30550 frames Cell Events: 1636 mean_circ: 0.936614
Ran for 31050 frames Cell Events: 1661 mean_circ: 0.936668
Ran for 31550 frames Cell Events: 1695 mean_circ: 0.936749
Ran for 32050 frames Cell Events: 1721 mean_circ: 0.936639
Ran for 33050 frames Cell Events: 1763 mean_circ: 0.936567
Ran for 33550 frames Cell Events: 1783 mean_circ: 0.936715
Ran for 34050 frames Cell Events: 1806 mean_circ: 0.936634
Ran for 35550 frames Cell Events: 1892 mean_circ: 0.936704
Ran for 36050 frames Cell Events: 1925 mean_circ: 0.936553
Ran for 37050 frames Cell Events: 1969 mean_circ: 0.936468
Ran for 38050 frames Cell Events: 2019 mean_circ: 0.936369
Ran for 38550 frames Cell Events: 2034 mean_circ: 0.936336
Ran for 39550 frames Cell Events: 2076 mean_circ: 0.936459
Ran for 40050 frames Cell Events: 2087 mean_circ: 0.936166
Ran for 40550 frames Cell Events: 2109 mean_circ: 0.936174
Ran for 41550 frames Cell Events: 2151 mean_circ: 0.936125
Ran for 42550 frames Cell Events: 2211 mean_circ: 0.936081
Ran for 43550 frames Cell Events: 2252 mean_circ: 0.936216
Ran for 44550 frames Cell Events: 2310 mean_circ: 0.936086
Ran for 45550 frames Cell Events: 2376 mean_circ: 0.93621
Ran for 46050 frames Cell Events: 2396 mean_circ: 0.936223
Ran for 46550 frames Cell Events: 2427 mean_circ: 0.936155
Ran for 47050 frames Cell Events: 2451 mean_circ: 0.936156
Ran for 47550 frames Cell Events: 2473 mean_circ: 0.936211
Ran for 48050 frames Cell Events: 2498 mean_circ: 0.93623
Ran for 48550 frames Cell Events: 2527 mean_circ: 0.936246
Ran for 49550 frames Cell Events: 2601 mean_circ: 0.936096
Ran for 50050 frames Cell Events: 2630 mean_circ: 0.936113
Ran for 50550 frames Cell Events: 2648 mean_circ: 0.935933
Ran for 51050 frames Cell Events: 2674 mean_circ: 0.935896
Ran for 52050 frames Cell Events: 2723 mean_circ: 0.936003
Ran for 52550 frames Cell Events: 2746 mean_circ: 0.935951
Ran for 53050 frames Cell Events: 2776 mean_circ: 0.935998
Ran for 53550 frames Cell Events: 2808 mean_circ: 0.93595
Ran for 54550 frames Cell Events: 2871 mean_circ: 0.936032
Ran for 55050 frames Cell Events: 2886 mean_circ: 0.93604
Ran for 55550 frames Cell Events: 2904 mean_circ: 0.936095
Ran for 57050 frames Cell Events: 2993 mean_circ: 0.936238
Ran for 58550 frames Cell Events: 3074 mean_circ: 0.936346
Ran for 59550 frames Cell Events: 3129 mean_circ: 0.936251
Ran for 60550 frames Cell Events: 3200 mean_circ: 0.936286
Ran for 61050 frames Cell Events: 3227 mean_circ: 0.936295
Ran for 62550 frames Cell Events: 3311 mean_circ: 0.936358
Ran for 63050 frames Cell Events: 3342 mean_circ: 0.936331
Ran for 63550 frames Cell Events: 3364 mean_circ: 0.936365
Ran for 64050 frames Cell Events: 3379 mean_circ: 0.93633
Ran for 64550 frames Cell Events: 3399 mean_circ: 0.936349
Ran for 65550 frames Cell Events: 3445 mean_circ: 0.936392
Ran for 66050 frames Cell Events: 3475 mean_circ: 0.936375
Ran for 68050 frames Cell Events: 3549 mean_circ: 0.936441
Ran for 70050 frames Cell Events: 3673 mean_circ: 0.936258
Ran for 72050 frames Cell Events: 3782 mean_circ: 0.936388
Ran for 76550 frames Cell Events: 4085 mean_circ: 0.936248
Ran for 77050 frames Cell Events: 4100 mean_circ: 0.936296
Ran for 77550 frames Cell Events: 4116 mean_circ: 0.93625
Ran for 80550 frames Cell Events: 4287 mean_circ: 0.936457
Ran for 81550 frames Cell Events: 4360 mean_circ: 0.936553
Ran for 82550 frames Cell Events: 4413 mean_circ: 0.936456
Ran for 84050 frames Cell Events: 4510 mean_circ: 0.936354
Ran for 85050 frames Cell Events: 4563 mean_circ: 0.936395
Ran for 87050 frames Cell Events: 4672 mean_circ: 0.936566
Ran for 90550 frames Cell Events: 4885 mean_circ: 0.936597
Ran for 94550 frames Cell Events: 5107 mean_circ: 0.936536
Ran for 95050 frames Cell Events: 5123 mean_circ: 0.936573
Ran for 96050 frames Cell Events: 5189 mean_circ: 0.936699
Ran for 97550 frames Cell Events: 5276 mean_circ: 0.936789
Ran for 100050 frames Cell Events: 5426 mean_circ: 0.936819
Ran for 100550 frames Cell Events: 5452 mean_circ: 0.936851
Ran for 101550 frames Cell Events: 5528 mean_circ: 0.936845
Ran for 103050 frames Cell Events: 5614 mean_circ: 0.936798
Ran for 104550 frames Cell Events: 5685 mean_circ: 0.936827
Ran for 105050 frames Cell Events: 5717 mean_circ: 0.936873
Ran for 105550 frames Cell Events: 5747 mean_circ: 0.936888
Ran for 106050 frames Cell Events: 5780 mean_circ: 0.936895
Ran for 106550 frames Cell Events: 5796 mean_circ: 0.936903
Ran for 107050 frames Cell Events: 5829 mean_circ: 0.93683
Ran for 109050 frames Cell Events: 5938 mean_circ: 0.936845
Ran for 109550 frames Cell Events: 5960 mean_circ: 0.936859
Ran for 110550 frames Cell Events: 6041 mean_circ: 0.936871
Ran for 111050 frames Cell Events: 6065 mean_circ: 0.936894
Ran for 111550 frames Cell Events: 6087 mean_circ: 0.93693
Ran for 112550 frames Cell Events: 6156 mean_circ: 0.936941
Ran for 113050 frames Cell Events: 6190 mean_circ: 0.936947
Ran for 114550 frames Cell Events: 6255 mean_circ: 0.936948
Ran for 115050 frames Cell Events: 6275 mean_circ: 0.936925
Ran for 116050 frames Cell Events: 6329 mean_circ: 0.936891
Ran for 116550 frames Cell Events: 6352 mean_circ: 0.936868
Ran for 117550 frames Cell Events: 6408 mean_circ: 0.936881
Ran for 119550 frames Cell Events: 6548 mean_circ: 0.936888
Ran for 120050 frames Cell Events: 6584 mean_circ: 0.936908
Ran for 121550 frames Cell Events: 6667 mean_circ: 0.936986
Ran for 122050 frames Cell Events: 6700 mean_circ: 0.937
Ran for 122550 frames Cell Events: 6725 mean_circ: 0.936973
Ran for 123050 frames Cell Events: 6749 mean_circ: 0.937016
Ran for 124050 frames Cell Events: 6812 mean_circ: 0.937047
Ran for 124550 frames Cell Events: 6833 mean_circ: 0.937077
Ran for 125050 frames Cell Events: 6856 mean_circ: 0.937112
Ran for 126050 frames Cell Events: 6907 mean_circ: 0.937194
Ran for 126550 frames Cell Events: 6944 mean_circ: 0.937177
Ran for 127050 frames Cell Events: 6973 mean_circ: 0.93712
Ran for 128550 frames Cell Events: 7059 mean_circ: 0.93711
Ran for 129550 frames Cell Events: 7126 mean_circ: 0.937005
Ran for 130050 frames Cell Events: 7160 mean_circ: 0.937022
Ran for 130550 frames Cell Events: 7192 mean_circ: 0.936972
Ran for 132550 frames Cell Events: 7308 mean_circ: 0.936957
Ran for 133050 frames Cell Events: 7335 mean_circ: 0.936893
Ran for 133550 frames Cell Events: 7358 mean_circ: 0.936855
Ran for 134050 frames Cell Events: 7389 mean_circ: 0.936906
Ran for 134550 frames Cell Events: 7430 mean_circ: 0.936912
Ran for 135550 frames Cell Events: 7485 mean_circ: 0.936931
Ran for 136050 frames Cell Events: 7514 mean_circ: 0.936986
Ran for 136550 frames Cell Events: 7552 mean_circ: 0.936986
Ran for 137050 frames Cell Events: 7583 mean_circ: 0.937
Ran for 141050 frames Cell Events: 7813 mean_circ: 0.937157
Ran for 141550 frames Cell Events: 7847 mean_circ: 0.937154
Ran for 142050 frames Cell Events: 7889 mean_circ: 0.937121
Ran for 145550 frames Cell Events: 8113 mean_circ: 0.937068
Ran for 146050 frames Cell Events: 8136 mean_circ: 0.937066



End this measurment..



Stopping time: 5.69898e+008
****************************************
Measurement finished

Mean Circularitiy: 0.93699
Mean Area: 668.316
Mean FL-1 intensity: 3343.87
Mean FL-2 intensity: 4923.56
Mean FL-3 intensity: 0

frames processed: 146375
Total Time: 73838.4ms
Number of Runs (new bg img) 0
Frames collected: 147505
Skipped frames: 62567
Rescued Frames: 3612942
Samples DAQ: 36876220
Samples written: 73752222
Samples per Channel: 36876111
FPS: 1997.67

Samples per second: 499417
Expected samples/s: 500000

Events: 8210
Events per second: 111.189
jumped back (PMT): 20
#Frames read, frequency
# 0 frames :225598
# 1 frames :56321
# 2 frames :3369
# 3 frames :1115
# 4 frames :636
# 5 frames :274
# 6 frames :2008
# 7 frames :1416
# 8 frames :915
# 9 frames :572
# 10 frames :427
# 11 frames :394
# 12 frames :379
# 13 frames :198
# 14 frames :200
# 15 frames :250
# 16 frames :148
# 17 frames :125
# 18 frames :94
# 19 frames :73
# 20 frames :63
# 21 frames :59
# 22 frames :48
# 23 frames :40
# 24 frames :24
# 25 frames :36
# 26 frames :28
# 27 frames :15
# 28 frames :8
# 29 frames :16
# 30 frames :11
# 31 frames :13
# 32 frames :15
# 33 frames :8
# 34 frames :8
# 35 frames :10
# 36 frames :6
# 37 frames :4
# 38 frames :5
# 39 frames :4
# 40 frames :4
# 41 frames :2
# 42 frames :3
# 43 frames :1
# 44 frames :2
# 45 frames :3
# 46 frames :4
# 47 frames :4
# 48 frames :2
# 49 frames :2
# 52 frames :1
# 60 frames :1
# 66 frames :1
# 68 frames :1
# 70 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.5294
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0