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Added the following fields to resource Figure4_Blood_DNN_Training_1.rtdc in Sorting real-time fluorescence and deformability cytometry (soRT-FDC) - manuscript data
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dc:fluorescence:channels installed with value
dc:online_filter:size_y soft limit with value
dc:online_contour:bin kernel with value
dc:experiment:run index with value
dc:fluorescence:trace median with value
dc:experiment:time with value
dc:experiment:sample with value
dc:fluorescence:signal max with value
dc:fluorescence:laser 3 power with value
dc:imaging:flash device with value
dc:fluorescence:laser 2 power with value
dc:fluorescence:samples per event with value
dc:online_filter:size_y max with value
dc:fluorescence:channel 2 name with value
dc:imaging:roi size x with value
dc:online_filter:size_y min with value
dc:imaging:frame rate with value
dc:online_contour:bin area min with value
dc:online_contour:bin threshold with value
dc:fluorescence:channel count with value
dc:imaging:roi position x with value
dc:online_filter:size_x min with value
dc:fluorescence:bit depth with value
dc:imaging:roi size y with value
dc:fluorescence:signal min with value
dc:setup:channel width with value
dc:fluorescence:sample rate with value
dc:experiment:date with value
dc:setup:module composition with value
dc:fluorescence:lasers installed with value
dc:setup:software version with value
dc:imaging:flash duration with value
dc:fluorescence:channel 3 name with value
dc:online_filter:size_x max with value
dc:imaging:roi position y with value
dc:fluorescence:channel 1 name with value
dc:online_contour:no absdiff with value
dc:imaging:pixel size with value
dc:setup:flow rate with value
dc:setup:identifier with value
dc:fluorescence:laser 3 lambda with value
dc:online_filter:size_x soft limit with value
dc:fluorescence:laser count with value
dc:setup:flow rate sheath with value
dc:fluorescence:laser 2 lambda with value
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dc:setup:chip region with value
dc:experiment:event count with value
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f | 1 | { | f | 1 | { |
2 | "authors": "Ahmad Ahsan Nawaz, Marta Urbanska, Maik Herbig, Martin | 2 | "authors": "Ahmad Ahsan Nawaz, Marta Urbanska, Maik Herbig, Martin | ||
3 | N\u00f6tzel, Martin Kr\u00e4ter, Philipp Rosendahl, Christoph Herold, | 3 | N\u00f6tzel, Martin Kr\u00e4ter, Philipp Rosendahl, Christoph Herold, | ||
4 | Nicole T\u00f6pfner, Marketa Kubankova, Ruchi Goswami, Shada | 4 | Nicole T\u00f6pfner, Marketa Kubankova, Ruchi Goswami, Shada | ||
5 | Abuhattum, Felix Reichel, Paul M\u00fcller, Anna Taubenberger, | 5 | Abuhattum, Felix Reichel, Paul M\u00fcller, Anna Taubenberger, | ||
6 | Salvatore Girardo, Angela Jacobi, Jochen Guck", | 6 | Salvatore Girardo, Angela Jacobi, Jochen Guck", | ||
7 | "creator_user_id": "d76d937b-3601-4559-92da-585a47c257f5", | 7 | "creator_user_id": "d76d937b-3601-4559-92da-585a47c257f5", | ||
8 | "doi": "10.6084/m9.figshare.11302595.v1", | 8 | "doi": "10.6084/m9.figshare.11302595.v1", | ||
9 | "groups": [], | 9 | "groups": [], | ||
10 | "id": "e61bb335-3523-e539-78d1-0720ad5a99f1", | 10 | "id": "e61bb335-3523-e539-78d1-0720ad5a99f1", | ||
11 | "isopen": true, | 11 | "isopen": true, | ||
12 | "license_id": "CC0-1.0", | 12 | "license_id": "CC0-1.0", | ||
13 | "license_title": "Creative Commons Public Domain Dedication", | 13 | "license_title": "Creative Commons Public Domain Dedication", | ||
14 | "license_url": "https://creativecommons.org/publicdomain/zero/1.0/", | 14 | "license_url": "https://creativecommons.org/publicdomain/zero/1.0/", | ||
15 | "metadata_created": "2020-09-16T15:59:26.382535", | 15 | "metadata_created": "2020-09-16T15:59:26.382535", | ||
n | 16 | "metadata_modified": "2020-09-16T16:33:09.296899", | n | 16 | "metadata_modified": "2020-09-16T16:33:10.170143", |
17 | "name": "figshare-11302595-v1", | 17 | "name": "figshare-11302595-v1", | ||
18 | "notes": "This dataset contains results presented in _Nawaz, | 18 | "notes": "This dataset contains results presented in _Nawaz, | ||
19 | Urbanska, Herbig_ et al\nmanuscript entitled \u2018 **Using real-time | 19 | Urbanska, Herbig_ et al\nmanuscript entitled \u2018 **Using real-time | ||
20 | fluorescence and deformability\ncytometry and deep learning to | 20 | fluorescence and deformability\ncytometry and deep learning to | ||
21 | transfer molecular specificity to label-free\nsorting** \u2019 | 21 | transfer molecular specificity to label-free\nsorting** \u2019 | ||
22 | available on bioRxiv (https://doi.org/10.1101/862227) | 22 | available on bioRxiv (https://doi.org/10.1101/862227) | ||
23 | and\nincludes:\n\n(i) 29 '*.rtdc' files containing measurements | 23 | and\nincludes:\n\n(i) 29 '*.rtdc' files containing measurements | ||
24 | performed with real time\nfluorescence and deformability cytometry | 24 | performed with real time\nfluorescence and deformability cytometry | ||
25 | [1,2]\n\n(ii) 1 '*.fcs' file containing a measurement performed with a | 25 | [1,2]\n\n(ii) 1 '*.fcs' file containing a measurement performed with a | ||
26 | standard flow\ncytometer (BD LSR II, BD Biosciences)\n\n(iii) 1 | 26 | standard flow\ncytometer (BD LSR II, BD Biosciences)\n\n(iii) 1 | ||
27 | '*.xlsx' file containing results from AFM-based | 27 | '*.xlsx' file containing results from AFM-based | ||
28 | indentation\nexperiments.\n\nSorting real-time fluorescence and | 28 | indentation\nexperiments.\n\nSorting real-time fluorescence and | ||
29 | deformability cytometry (soRT-FDC) is a\nrobust sorting platform, | 29 | deformability cytometry (soRT-FDC) is a\nrobust sorting platform, | ||
30 | combining image-based morphological cell analysis\nwith mechanical | 30 | combining image-based morphological cell analysis\nwith mechanical | ||
31 | characterisation and subsequent active sorting by feeding\nreal-time | 31 | characterisation and subsequent active sorting by feeding\nreal-time | ||
32 | fluorescence and deformability cytometry (RT-FDC) [2] information | 32 | fluorescence and deformability cytometry (RT-FDC) [2] information | ||
33 | to\na down-stream SSAW-based cell sorter [3].\n\nEach filename of this | 33 | to\na down-stream SSAW-based cell sorter [3].\n\nEach filename of this | ||
34 | dataset starts with the name of the figure to which the\ndatafile | 34 | dataset starts with the name of the figure to which the\ndatafile | ||
35 | corresponds. The measured samples are briefly described below. | 35 | corresponds. The measured samples are briefly described below. | ||
36 | The\n'Initial' measurements refer to the samples used for sorting, and | 36 | The\n'Initial' measurements refer to the samples used for sorting, and | ||
37 | the 'Target'\nmeasurements to the samples collected in the target | 37 | the 'Target'\nmeasurements to the samples collected in the target | ||
38 | outlet after sorting. For\nmore detailed description of samples please | 38 | outlet after sorting. For\nmore detailed description of samples please | ||
39 | refer to the manuscript.\n\nThe '*.rtdc' files are HDF5 files and can | 39 | refer to the manuscript.\n\nThe '*.rtdc' files are HDF5 files and can | ||
40 | be analysed using a Python library\ncalled dclab [4], or a software | 40 | be analysed using a Python library\ncalled dclab [4], or a software | ||
41 | called Shape-Out [5]. They can also be opened\nusing other HDF viewer | 41 | called Shape-Out [5]. They can also be opened\nusing other HDF viewer | ||
42 | programs.\n\n[1] Otto et al., \"Real-time deformability cytometry: | 42 | programs.\n\n[1] Otto et al., \"Real-time deformability cytometry: | ||
43 | on-the-fly cell\nmechanical phenotyping\". Nature Methods, | 43 | on-the-fly cell\nmechanical phenotyping\". Nature Methods, | ||
44 | 12(3):199\u2013202, 2015.\ndoi:10.1038/nmeth.3281.\n\n[2] Rosendahl et | 44 | 12(3):199\u2013202, 2015.\ndoi:10.1038/nmeth.3281.\n\n[2] Rosendahl et | ||
45 | al., \"Real-time fluorescence and deformability cytometry\".\nNature | 45 | al., \"Real-time fluorescence and deformability cytometry\".\nNature | ||
46 | Methods, 15(5):355\u2013358, 2018. doi:10.1038/nmeth.4639.\n\n[3] | 46 | Methods, 15(5):355\u2013358, 2018. doi:10.1038/nmeth.4639.\n\n[3] | ||
47 | Nawaz et al., \u201cAcoustofluidic Fluorescence Activated Cell | 47 | Nawaz et al., \u201cAcoustofluidic Fluorescence Activated Cell | ||
48 | Sorter\u201d.\nAnalitycial Chemistry 87(24): 12051\u201312058, 2015. | 48 | Sorter\u201d.\nAnalitycial Chemistry 87(24): 12051\u201312058, 2015. | ||
49 | doi:\ndoi.org/10.1021/acs.analchem.5b02398\n\n[4] | 49 | doi:\ndoi.org/10.1021/acs.analchem.5b02398\n\n[4] | ||
50 | https://github.com/ZellMechanik-Dresden/dclab\n\n[5] | 50 | https://github.com/ZellMechanik-Dresden/dclab\n\n[5] | ||
51 | https://github.com/ZellMechanik-Dresden/ShapeOut\n\n | 51 | https://github.com/ZellMechanik-Dresden/ShapeOut\n\n | ||
52 | \n\n**_Figure1d_01_Beads_FL_Initial.rtdc, | 52 | \n\n**_Figure1d_01_Beads_FL_Initial.rtdc, | ||
53 | Figure1d_02_Beads_FL_Target.rtdc_**\n\nsample: 1:5 mixture of | 53 | Figure1d_02_Beads_FL_Target.rtdc_**\n\nsample: 1:5 mixture of | ||
54 | polyacrylamide microgel beads labeled with\nAlexaFluor488 and | 54 | polyacrylamide microgel beads labeled with\nAlexaFluor488 and | ||
55 | unlabeled ones, these beads were produced in house; sorted\nfor | 55 | unlabeled ones, these beads were produced in house; sorted\nfor | ||
56 | fluorescence\n\nsorting gates: FL-1 maximum 1000 \u2013 10000, area | 56 | fluorescence\n\nsorting gates: FL-1 maximum 1000 \u2013 10000, area | ||
57 | ratio: 1.0 - 1.1\n\n \n\n**_Figure1d_03_Beads_Size_Initial.rtdc, | 57 | ratio: 1.0 - 1.1\n\n \n\n**_Figure1d_03_Beads_Size_Initial.rtdc, | ||
58 | Figure1d_04_Beads_Size_Target.rtdc_**\n\nsample: 1:6 mixture of 13.79 | 58 | Figure1d_04_Beads_Size_Target.rtdc_**\n\nsample: 1:6 mixture of 13.79 | ||
59 | \u00b1 0.59 \u03bcm silica beads (SiO2-F-L3519-1;\nMicroparticles, | 59 | \u00b1 0.59 \u03bcm silica beads (SiO2-F-L3519-1;\nMicroparticles, | ||
60 | Germany) and 17.23 \u00b1 0.24 \u03bcm poly(methyl methacrylate) | 60 | Germany) and 17.23 \u00b1 0.24 \u03bcm poly(methyl methacrylate) | ||
61 | beads\n(PMMM-F-B1423; Microparticles); sorted for size\n\nsorting | 61 | beads\n(PMMM-F-B1423; Microparticles); sorted for size\n\nsorting | ||
62 | gates: size 220 \u2013 300 \u03bcm2, def 0.00 \u2013 0.01, are ratio | 62 | gates: size 220 \u2013 300 \u03bcm2, def 0.00 \u2013 0.01, are ratio | ||
63 | 1.0 \u2013 1.1\n\n | 63 | 1.0 \u2013 1.1\n\n | ||
64 | ize_Initial.rtdc,\nFigure1d_06_Beads_DefSize_Target.rtdc_**\n\nsample: | 64 | ize_Initial.rtdc,\nFigure1d_06_Beads_DefSize_Target.rtdc_**\n\nsample: | ||
65 | a mixture of two polyacrylamide microgel bead populations of | 65 | a mixture of two polyacrylamide microgel bead populations of | ||
66 | different\nstiffness, these beads were produced in house; sorted for | 66 | different\nstiffness, these beads were produced in house; sorted for | ||
67 | deformation and size\n\nsorting gates: size 95 \u2013 105 \u03bcm2, | 67 | deformation and size\n\nsorting gates: size 95 \u2013 105 \u03bcm2, | ||
68 | def 0.000 \u2013 0.016, are ratio 1.0 \u2013 1.1\n\n | 68 | def 0.000 \u2013 0.016, are ratio 1.0 \u2013 1.1\n\n | ||
69 | \n\n**_Figure2b_Blood_Initial_Inlet.rtdc_**\n\nsample: RBC-depleted | 69 | \n\n**_Figure2b_Blood_Initial_Inlet.rtdc_**\n\nsample: RBC-depleted | ||
70 | blood sample analysed in the inlet region of the chip\n\n | 70 | blood sample analysed in the inlet region of the chip\n\n | ||
71 | \n\n**_Figure2c_Blood_Initial_Channel.rtdc_**\n\nsample: RBC-depleted | 71 | \n\n**_Figure2c_Blood_Initial_Channel.rtdc_**\n\nsample: RBC-depleted | ||
72 | blood sample analysed in the usual ROI at the end of the\nconstricting | 72 | blood sample analysed in the usual ROI at the end of the\nconstricting | ||
73 | channel\n\n \n\n**_Figure2d_Blood_Target_RBCs.rtdc_**\n\nsample: | 73 | channel\n\n \n\n**_Figure2d_Blood_Target_RBCs.rtdc_**\n\nsample: | ||
74 | RBC-depleted blood, sorted RBCs\n\nsorting gates: size 25 \u2013 65 | 74 | RBC-depleted blood, sorted RBCs\n\nsorting gates: size 25 \u2013 65 | ||
75 | \u03bcm2, def 0.16 \u2013 0.40, are ratio 1.0 \u2013 1.1\n\n | 75 | \u03bcm2, def 0.16 \u2013 0.40, are ratio 1.0 \u2013 1.1\n\n | ||
76 | \n\n**_Figure2e_Blood_Target_ly.rtdc_**\n\nsample: RBC-depleted blood, | 76 | \n\n**_Figure2e_Blood_Target_ly.rtdc_**\n\nsample: RBC-depleted blood, | ||
77 | sorted lymphocytes\n\nsorting gates: size 25 \u2013 45 \u03bcm2, def | 77 | sorted lymphocytes\n\nsorting gates: size 25 \u2013 45 \u03bcm2, def | ||
78 | 0.00 \u2013 0.10, are ratio 1.0 \u2013 1.1\n\n | 78 | 0.00 \u2013 0.10, are ratio 1.0 \u2013 1.1\n\n | ||
79 | \n\n**_Figure2f_Blood_Target_my.rtdc_**\n\nsample: RBC-depleted blood, | 79 | \n\n**_Figure2f_Blood_Target_my.rtdc_**\n\nsample: RBC-depleted blood, | ||
80 | sorted myeloid cells\n\nsorting gates: size 53 \u2013 120 \u03bcm2, | 80 | sorted myeloid cells\n\nsorting gates: size 53 \u2013 120 \u03bcm2, | ||
81 | def 0.00 \u2013 0.15, are ratio 1.0 \u2013 1.1\n\n | 81 | def 0.00 \u2013 0.15, are ratio 1.0 \u2013 1.1\n\n | ||
82 | \n\n**_Figure3_Blood_Initial.rtdc, | 82 | \n\n**_Figure3_Blood_Initial.rtdc, | ||
83 | Figure3_Blood_Target_neuBr.rtdc_**\n\nsample: RBC-depleted blood, | 83 | Figure3_Blood_Target_neuBr.rtdc_**\n\nsample: RBC-depleted blood, | ||
84 | brightness-based neutrophils sorting*\n\nsorting gates: size 56 \u2013 | 84 | brightness-based neutrophils sorting*\n\nsorting gates: size 56 \u2013 | ||
85 | 100 \u03bcm2, brightness 75 \u2013 78, are ratio 1.0 \u2013 1.1\n\n | 85 | 100 \u03bcm2, brightness 75 \u2013 78, are ratio 1.0 \u2013 1.1\n\n | ||
86 | \n\n**_Figure4_Blood_DNN_Training(1).rtdc, | 86 | \n\n**_Figure4_Blood_DNN_Training(1).rtdc, | ||
87 | Figure4_Blood_DNN_Training(2).rtdc_**\n\nsample: RBC-depleted blood, | 87 | Figure4_Blood_DNN_Training(2).rtdc_**\n\nsample: RBC-depleted blood, | ||
88 | datasets for training of deep neural network\n(DNN)*\n\n | 88 | datasets for training of deep neural network\n(DNN)*\n\n | ||
89 | \n\n**_Figure4_Blood_DNN_Validation.rtdc_**\n\nsample: RBC-depleted | 89 | \n\n**_Figure4_Blood_DNN_Validation.rtdc_**\n\nsample: RBC-depleted | ||
90 | blood, dataset for validation of trained DNN*\n\n | 90 | blood, dataset for validation of trained DNN*\n\n | ||
91 | \n\n**_Figure4d_Blood_Initial.rtdc, | 91 | \n\n**_Figure4d_Blood_Initial.rtdc, | ||
92 | Figure4e_Blood_Target_neuDNN.rtdc_**\n\nsample: RBC-depleted blood, | 92 | Figure4e_Blood_Target_neuDNN.rtdc_**\n\nsample: RBC-depleted blood, | ||
93 | DNN-based neutrophil sorting*\n\n \n\n*for identification of | 93 | DNN-based neutrophil sorting*\n\n \n\n*for identification of | ||
94 | neutrophils (CD66+/CD14\u2212), these samples were stained with | 94 | neutrophils (CD66+/CD14\u2212), these samples were stained with | ||
95 | APC-conjugated anti-human CD14 (dilution 1:20, #17- 809 0149-42, | 95 | APC-conjugated anti-human CD14 (dilution 1:20, #17- 809 0149-42, | ||
96 | eBioscience, CA, USA; recorded with FL-3 channel) and PE-conjugated | 96 | eBioscience, CA, USA; recorded with FL-3 channel) and PE-conjugated | ||
97 | anti-human CD66a/c/e (dilution 1:40, 810 #34303, BioLegend, CA, USA; | 97 | anti-human CD66a/c/e (dilution 1:40, 810 #34303, BioLegend, CA, USA; | ||
98 | recorded with FL-2 channel); for cross-talk compensation of the | 98 | recorded with FL-2 channel); for cross-talk compensation of the | ||
99 | fluorescence signal in channels FL-2 and FL-3 for these samples | 99 | fluorescence signal in channels FL-2 and FL-3 for these samples | ||
100 | introduce the following correction factor in the ShapeOut software: | 100 | introduce the following correction factor in the ShapeOut software: | ||
101 | spill from channel 2 to 3 = 0.025, spill from channel 3 to 2 = | 101 | spill from channel 2 to 3 = 0.025, spill from channel 3 to 2 = | ||
102 | 0.100\n\n \n\n**_SFigure2a_BeadMix_RT-FDC.rtdc, | 102 | 0.100\n\n \n\n**_SFigure2a_BeadMix_RT-FDC.rtdc, | ||
103 | igure2b_BeadMix_soRT-FDC.rtdc,\nSFigure2c_BeadMix_FC.fcs_**\n\nsample: | 103 | igure2b_BeadMix_soRT-FDC.rtdc,\nSFigure2c_BeadMix_FC.fcs_**\n\nsample: | ||
104 | a mixture of 4 bead types with different diameter, each made | 104 | a mixture of 4 bead types with different diameter, each made | ||
105 | of\ndifferent material:\n\n9.78 \u00b1 0.15 \u03bcm melamine beads | 105 | of\ndifferent material:\n\n9.78 \u00b1 0.15 \u03bcm melamine beads | ||
106 | (MF-FluoBlau-L948),\n\n13.79 \u00b1 0.59 \u03bcm silica beads | 106 | (MF-FluoBlau-L948),\n\n13.79 \u00b1 0.59 \u03bcm silica beads | ||
107 | (SiO2-F-L3519-1),\n\n15.21 \u00b1 0.31 \u03bcm polystyrene beads | 107 | (SiO2-F-L3519-1),\n\n15.21 \u00b1 0.31 \u03bcm polystyrene beads | ||
108 | (PS/Q-F-KM194),\n\n17.23 \u00b1 0.24 \u03bcm poly(methyl methacrylate) | 108 | (PS/Q-F-KM194),\n\n17.23 \u00b1 0.24 \u03bcm poly(methyl methacrylate) | ||
109 | beads (PMMM-F-B1423),\n\nall purchased from Microparticles, | 109 | beads (PMMM-F-B1423),\n\nall purchased from Microparticles, | ||
110 | Germany.\n\n \n\n**_SFigure3a_Beads_FLDef_Initial.rtdc, | 110 | Germany.\n\n \n\n**_SFigure3a_Beads_FLDef_Initial.rtdc, | ||
111 | SFigure3a_Beads_FLDef_Target.rtdc_**\n\nsample: a mixture of | 111 | SFigure3a_Beads_FLDef_Target.rtdc_**\n\nsample: a mixture of | ||
112 | fluorescent and non-fluorescent polyacrylamide beads with\ndifferent | 112 | fluorescent and non-fluorescent polyacrylamide beads with\ndifferent | ||
113 | mechanical properties, these beads were produced in house; sorted\nfor | 113 | mechanical properties, these beads were produced in house; sorted\nfor | ||
114 | fluorescence and deformation\n\nsorting gates: FL-1 maximum 1000 | 114 | fluorescence and deformation\n\nsorting gates: FL-1 maximum 1000 | ||
115 | \u2013 10000, def 0.01 \u2013 0.02, are ratio 1.0 \u2013 1.1\n\n | 115 | \u2013 10000, def 0.01 \u2013 0.02, are ratio 1.0 \u2013 1.1\n\n | ||
116 | \n\n**_SFigure3b_Beads_BrSize_Initial.rtdc, | 116 | \n\n**_SFigure3b_Beads_BrSize_Initial.rtdc, | ||
117 | SFigure3b_Beads_BrSize_Target.rtdc_**\n\nsample: a mixture of 4 bead | 117 | SFigure3b_Beads_BrSize_Target.rtdc_**\n\nsample: a mixture of 4 bead | ||
118 | types as in SFigure2; sorted for brightness and\nsize\n\nsorting | 118 | types as in SFigure2; sorted for brightness and\nsize\n\nsorting | ||
119 | gates: size 160 \u2013 240 \u03bcm2, brightness 75 \u2013 85, are | 119 | gates: size 160 \u2013 240 \u03bcm2, brightness 75 \u2013 85, are | ||
120 | ratio 1.0 \u2013 1.1\n\n | 120 | ratio 1.0 \u2013 1.1\n\n | ||
121 | ize_Initial.rtdc,\nSFigure4b_Kc167_HL60_Size_Target.rtdc_**\n\nsample: | 121 | ize_Initial.rtdc,\nSFigure4b_Kc167_HL60_Size_Target.rtdc_**\n\nsample: | ||
122 | a mixture of Kc167 _Drosophila_ cells and HL60/S4 human | 122 | a mixture of Kc167 _Drosophila_ cells and HL60/S4 human | ||
123 | promyelocytic\nleukaemia cells, sorted for size\n\nsorting gates: size | 123 | promyelocytic\nleukaemia cells, sorted for size\n\nsorting gates: size | ||
124 | 25 \u2013 77 \u03bcm2, def 0.00 \u2013 0.15, are ratio 1.0 \u2013 | 124 | 25 \u2013 77 \u03bcm2, def 0.00 \u2013 0.15, are ratio 1.0 \u2013 | ||
125 | 1.1\n\n \n\n**_SFigure5a_RBC_Def_Initial.rtdc, | 125 | 1.1\n\n \n\n**_SFigure5a_RBC_Def_Initial.rtdc, | ||
126 | 5b_RBC_Def_Target1.rtdc,\nSFigure5c_RBC_Def_Target2.rtdc_**\n\nsample: | 126 | 5b_RBC_Def_Target1.rtdc,\nSFigure5c_RBC_Def_Target2.rtdc_**\n\nsample: | ||
127 | blood anticoagulated with citrate, RBCs sorted for | 127 | blood anticoagulated with citrate, RBCs sorted for | ||
128 | deformation\n\nsorting gates: def 0.15 \u2013 0.40 (Target1) / def | 128 | deformation\n\nsorting gates: def 0.15 \u2013 0.40 (Target1) / def | ||
129 | 0.00 \u2013 0.10 (Target2), are\nratio 1.0 \u2013 1.2\n\n | 129 | 0.00 \u2013 0.10 (Target2), are\nratio 1.0 \u2013 1.2\n\n | ||
130 | \n\n**_SFigure7_AFM_AfterSorting.xlsx_**\n\nsample: HL60/S4 cells | 130 | \n\n**_SFigure7_AFM_AfterSorting.xlsx_**\n\nsample: HL60/S4 cells | ||
131 | collected after sorting experiment; the initial sample\nwas prepared | 131 | collected after sorting experiment; the initial sample\nwas prepared | ||
132 | as for the soRT-FDC experiment but not loaded onto the chip, | 132 | as for the soRT-FDC experiment but not loaded onto the chip, | ||
133 | the\ndefault sample was collected in the default outlet, i.e., run | 133 | the\ndefault sample was collected in the default outlet, i.e., run | ||
134 | through the\nsorting chip but not exposed to SSAW, and the target | 134 | through the\nsorting chip but not exposed to SSAW, and the target | ||
135 | sample corresponds to\ncells exposed to SSAW; reported values | 135 | sample corresponds to\ncells exposed to SSAW; reported values | ||
136 | correspond to apparent Young\u2019s modulus\nestimated from AFM | 136 | correspond to apparent Young\u2019s modulus\nestimated from AFM | ||
137 | indentation experiments\n\n \n\n", | 137 | indentation experiments\n\n \n\n", | ||
138 | "num_resources": 32, | 138 | "num_resources": 32, | ||
139 | "num_tags": 0, | 139 | "num_tags": 0, | ||
140 | "organization": { | 140 | "organization": { | ||
141 | "approval_status": "approved", | 141 | "approval_status": "approved", | ||
142 | "created": "2020-09-16T15:58:45.009115", | 142 | "created": "2020-09-16T15:58:45.009115", | ||
143 | "description": "This lab contains selected data imported from | 143 | "description": "This lab contains selected data imported from | ||
144 | figshare. If you would like your dataset to appear here, please send | 144 | figshare. If you would like your dataset to appear here, please send | ||
145 | the figshare DOI to Paul M\u00fcller.", | 145 | the figshare DOI to Paul M\u00fcller.", | ||
146 | "id": "5a7a053d-55fb-4f99-960c-f478d0bd418f", | 146 | "id": "5a7a053d-55fb-4f99-960c-f478d0bd418f", | ||
147 | "image_url": "", | 147 | "image_url": "", | ||
148 | "is_organization": true, | 148 | "is_organization": true, | ||
149 | "name": "figshare-import", | 149 | "name": "figshare-import", | ||
150 | "state": "active", | 150 | "state": "active", | ||
151 | "title": "Figshare mirror", | 151 | "title": "Figshare mirror", | ||
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