Changes
On September 16, 2020 at 7:35:21 PM +0200, not logged in:
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Added the following fields to resource 2016-03-02_M6_reservoir_efe92abf.rtdc in Polyacrylamide beads measured with RT-DC at different flow rates
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dc:setup:flow rate with value
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f | 1 | { | f | 1 | { |
2 | "authors": "Paul M\u00fcller, Christoph Herold, Maik Herbig, Jochen | 2 | "authors": "Paul M\u00fcller, Christoph Herold, Maik Herbig, Jochen | ||
3 | Guck", | 3 | Guck", | ||
4 | "creator_user_id": "d76d937b-3601-4559-92da-585a47c257f5", | 4 | "creator_user_id": "d76d937b-3601-4559-92da-585a47c257f5", | ||
5 | "doi": "10.6084/m9.figshare.12721436.v1", | 5 | "doi": "10.6084/m9.figshare.12721436.v1", | ||
6 | "groups": [], | 6 | "groups": [], | ||
7 | "id": "c7a98a04-4e0a-98a7-fb0b-eca379d1f219", | 7 | "id": "c7a98a04-4e0a-98a7-fb0b-eca379d1f219", | ||
8 | "isopen": true, | 8 | "isopen": true, | ||
9 | "license_id": "CC0-1.0", | 9 | "license_id": "CC0-1.0", | ||
10 | "license_title": "Creative Commons Public Domain Dedication", | 10 | "license_title": "Creative Commons Public Domain Dedication", | ||
11 | "license_url": "https://creativecommons.org/publicdomain/zero/1.0/", | 11 | "license_url": "https://creativecommons.org/publicdomain/zero/1.0/", | ||
12 | "metadata_created": "2020-09-16T16:02:44.208067", | 12 | "metadata_created": "2020-09-16T16:02:44.208067", | ||
n | 13 | "metadata_modified": "2020-09-16T17:35:21.186693", | n | 13 | "metadata_modified": "2020-09-16T17:35:21.860684", |
14 | "name": "figshare-12721436-v1", | 14 | "name": "figshare-12721436-v1", | ||
15 | "notes": "Polyacrylamide hydrogel beads from the same production | 15 | "notes": "Polyacrylamide hydrogel beads from the same production | ||
16 | batch were measured\nwith real-time deformability cytometry (RT-DC) at | 16 | batch were measured\nwith real-time deformability cytometry (RT-DC) at | ||
17 | different flow rates (0.04,\n0.08, and 0.12 \u00b5L/s). The beads were | 17 | different flow rates (0.04,\n0.08, and 0.12 \u00b5L/s). The beads were | ||
18 | designed to have a Young's modulus of\napproximately 4.3 \u00b1 1.5 | 18 | designed to have a Young's modulus of\napproximately 4.3 \u00b1 1.5 | ||
19 | kPa. These measurements were used to illustrate the\ncorrection of | 19 | kPa. These measurements were used to illustrate the\ncorrection of | ||
20 | systematic errors for the computation of the Young's modulus in\nRT-DC | 20 | systematic errors for the computation of the Young's modulus in\nRT-DC | ||
21 | (figure 10 in [1]). The reservoir measurement was added for the sake | 21 | (figure 10 in [1]). The reservoir measurement was added for the sake | ||
22 | of\ncompleteness. The files were converted from the .tdms file format | 22 | of\ncompleteness. The files were converted from the .tdms file format | ||
23 | to the .rtdc\nfile format using DCKit 0.10.0 [2] and dclab 0.27.8 [3]. | 23 | to the .rtdc\nfile format using DCKit 0.10.0 [2] and dclab 0.27.8 [3]. | ||
24 | \n \nBefore analysis, the data should be filtered for porosity (1.0 < | 24 | \n \nBefore analysis, the data should be filtered for porosity (1.0 < | ||
25 | area_ratio <\n1.1) and deformation (0 < deform < 0.035). This is | 25 | area_ratio <\n1.1) and deformation (0 < deform < 0.035). This is | ||
26 | necessary, because events\nwere not always detected correctly | 26 | necessary, because events\nwere not always detected correctly | ||
27 | (incorrect contour data) due to lack of\nimaging contrast. \n \n \n | 27 | (incorrect contour data) due to lack of\nimaging contrast. \n \n \n | ||
28 | **References:** \n[1] Christoph Herold. Mapping of Deformation to | 28 | **References:** \n[1] Christoph Herold. Mapping of Deformation to | ||
29 | Apparent Young\u2019s Modulus in\nReal-Time Deformability Cytometry. | 29 | Apparent Young\u2019s Modulus in\nReal-Time Deformability Cytometry. | ||
30 | ArXiv e-prints 1704.00572 [cond-mat.soft],\n2017. arXiv:1704.00572v1. | 30 | ArXiv e-prints 1704.00572 [cond-mat.soft],\n2017. arXiv:1704.00572v1. | ||
31 | \n[2] https://github.com/ZELLMECHANIK-DRESDEN/DCKit \n[3] | 31 | \n[2] https://github.com/ZELLMECHANIK-DRESDEN/DCKit \n[3] | ||
32 | https://github.com/ZellMechanik-Dresden/dclab \n \n \n **SHA256 | 32 | https://github.com/ZellMechanik-Dresden/dclab \n \n \n **SHA256 | ||
33 | sums:** | 33 | sums:** | ||
34 | cc61d10c89969c1f8fa2c55ec933b35\n2016-03-02_M3_0.0400uls_22f26fe5.rtdc | 34 | cc61d10c89969c1f8fa2c55ec933b35\n2016-03-02_M3_0.0400uls_22f26fe5.rtdc | ||
35 | 9e39414ab9d4ccafa3097d229e2e31d\n2016-03-02_M4_0.0800uls_1e1e8234.rtdc | 35 | 9e39414ab9d4ccafa3097d229e2e31d\n2016-03-02_M4_0.0800uls_1e1e8234.rtdc | ||
36 | b3d5f5e8d3ec32d641aa4f26d09cdd0\n2016-03-02_M5_0.1200uls_d387ab27.rtdc | 36 | b3d5f5e8d3ec32d641aa4f26d09cdd0\n2016-03-02_M5_0.1200uls_d387ab27.rtdc | ||
37 | a71e12f7c0d41bbf0545e0a02\n2016-03-02_M6_reservoir_efe92abf.rtdc\n\n", | 37 | a71e12f7c0d41bbf0545e0a02\n2016-03-02_M6_reservoir_efe92abf.rtdc\n\n", | ||
38 | "num_resources": 5, | 38 | "num_resources": 5, | ||
39 | "num_tags": 0, | 39 | "num_tags": 0, | ||
40 | "organization": { | 40 | "organization": { | ||
41 | "approval_status": "approved", | 41 | "approval_status": "approved", | ||
42 | "created": "2020-09-16T15:58:45.009115", | 42 | "created": "2020-09-16T15:58:45.009115", | ||
43 | "description": "This lab contains selected data imported from | 43 | "description": "This lab contains selected data imported from | ||
44 | figshare. If you would like your dataset to appear here, please send | 44 | figshare. If you would like your dataset to appear here, please send | ||
45 | the figshare DOI to Paul M\u00fcller.", | 45 | the figshare DOI to Paul M\u00fcller.", | ||
46 | "id": "5a7a053d-55fb-4f99-960c-f478d0bd418f", | 46 | "id": "5a7a053d-55fb-4f99-960c-f478d0bd418f", | ||
47 | "image_url": "", | 47 | "image_url": "", | ||
48 | "is_organization": true, | 48 | "is_organization": true, | ||
49 | "name": "figshare-import", | 49 | "name": "figshare-import", | ||
50 | "state": "active", | 50 | "state": "active", | ||
51 | "title": "Figshare mirror", | 51 | "title": "Figshare mirror", | ||
52 | "type": "organization" | 52 | "type": "organization" | ||
53 | }, | 53 | }, | ||
54 | "owner_org": "5a7a053d-55fb-4f99-960c-f478d0bd418f", | 54 | "owner_org": "5a7a053d-55fb-4f99-960c-f478d0bd418f", | ||
55 | "private": false, | 55 | "private": false, | ||
56 | "references": "arxiv:1704.00572, | 56 | "references": "arxiv:1704.00572, | ||
57 | https://github.com/ZELLMECHANIK-DRESDEN/DCKit, | 57 | https://github.com/ZELLMECHANIK-DRESDEN/DCKit, | ||
58 | https://github.com/ZellMechanik-Dresden/dclab", | 58 | https://github.com/ZellMechanik-Dresden/dclab", | ||
59 | "relationships_as_object": [], | 59 | "relationships_as_object": [], | ||
60 | "relationships_as_subject": [], | 60 | "relationships_as_subject": [], | ||
61 | "resources": [ | 61 | "resources": [ | ||
62 | { | 62 | { | ||
63 | "cache_last_updated": null, | 63 | "cache_last_updated": null, | ||
64 | "cache_url": null, | 64 | "cache_url": null, | ||
65 | "created": "2020-09-16T16:02:45.190901", | 65 | "created": "2020-09-16T16:02:45.190901", | ||
66 | "dc:experiment:date": "2016-03-03", | 66 | "dc:experiment:date": "2016-03-03", | ||
67 | "dc:experiment:event count": 4544, | 67 | "dc:experiment:event count": 4544, | ||
68 | "dc:experiment:run index": 3, | 68 | "dc:experiment:run index": 3, | ||
69 | "dc:experiment:sample": "S1 beads | 69 | "dc:experiment:sample": "S1 beads | ||
70 | 0.04\u2009\u00b5Ls\u207b\u00b9", | 70 | 0.04\u2009\u00b5Ls\u207b\u00b9", | ||
71 | "dc:experiment:time": "15:01:00", | 71 | "dc:experiment:time": "15:01:00", | ||
72 | "dc:imaging:flash device": "LED (undefined)", | 72 | "dc:imaging:flash device": "LED (undefined)", | ||
73 | "dc:imaging:flash duration": 2.0, | 73 | "dc:imaging:flash duration": 2.0, | ||
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79 | "dc:imaging:roi size y": 80, | 79 | "dc:imaging:roi size y": 80, | ||
80 | "dc:online_contour:bin area min": 50, | 80 | "dc:online_contour:bin area min": 50, | ||
81 | "dc:online_contour:bin kernel": 5, | 81 | "dc:online_contour:bin kernel": 5, | ||
82 | "dc:online_contour:bin threshold": 3, | 82 | "dc:online_contour:bin threshold": 3, | ||
83 | "dc:online_contour:image blur": 0, | 83 | "dc:online_contour:image blur": 0, | ||
84 | "dc:online_contour:no absdiff": true, | 84 | "dc:online_contour:no absdiff": true, | ||
85 | "dc:setup:channel width": 20.0, | 85 | "dc:setup:channel width": 20.0, | ||
86 | "dc:setup:chip region": "channel", | 86 | "dc:setup:chip region": "channel", | ||
87 | "dc:setup:flow rate": 0.04, | 87 | "dc:setup:flow rate": 0.04, | ||
88 | "dc:setup:flow rate sample": 0.01, | 88 | "dc:setup:flow rate sample": 0.01, | ||
89 | "dc:setup:flow rate sheath": 0.03, | 89 | "dc:setup:flow rate sheath": 0.03, | ||
90 | "dc:setup:identifier": "RTDC1", | 90 | "dc:setup:identifier": "RTDC1", | ||
91 | "dc:setup:medium": "CellCarrier", | 91 | "dc:setup:medium": "CellCarrier", | ||
92 | "dc:setup:module composition": "AcCellerator", | 92 | "dc:setup:module composition": "AcCellerator", | ||
93 | "dc:setup:software version": "dclab 0.27.8 | dclab 0.27.8", | 93 | "dc:setup:software version": "dclab 0.27.8 | dclab 0.27.8", | ||
94 | "dc:setup:temperature": 23.0, | 94 | "dc:setup:temperature": 23.0, | ||
95 | "description": null, | 95 | "description": null, | ||
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113 | "url_type": "upload" | 113 | "url_type": "upload" | ||
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115 | { | 115 | { | ||
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118 | "created": "2020-09-16T16:02:47.217831", | 118 | "created": "2020-09-16T16:02:47.217831", | ||
119 | "dc:experiment:date": "2016-03-03", | 119 | "dc:experiment:date": "2016-03-03", | ||
120 | "dc:experiment:event count": 5006, | 120 | "dc:experiment:event count": 5006, | ||
121 | "dc:experiment:run index": 4, | 121 | "dc:experiment:run index": 4, | ||
122 | "dc:experiment:sample": "S1 beads | 122 | "dc:experiment:sample": "S1 beads | ||
123 | 0.08\u2009\u00b5Ls\u207b\u00b9", | 123 | 0.08\u2009\u00b5Ls\u207b\u00b9", | ||
124 | "dc:experiment:time": "15:03:00", | 124 | "dc:experiment:time": "15:03:00", | ||
125 | "dc:imaging:flash device": "LED (undefined)", | 125 | "dc:imaging:flash device": "LED (undefined)", | ||
126 | "dc:imaging:flash duration": 2.0, | 126 | "dc:imaging:flash duration": 2.0, | ||
127 | "dc:imaging:frame rate": 2000.0, | 127 | "dc:imaging:frame rate": 2000.0, | ||
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134 | "dc:online_contour:bin kernel": 5, | 134 | "dc:online_contour:bin kernel": 5, | ||
135 | "dc:online_contour:bin threshold": 3, | 135 | "dc:online_contour:bin threshold": 3, | ||
136 | "dc:online_contour:image blur": 0, | 136 | "dc:online_contour:image blur": 0, | ||
137 | "dc:online_contour:no absdiff": true, | 137 | "dc:online_contour:no absdiff": true, | ||
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139 | "dc:setup:chip region": "channel", | 139 | "dc:setup:chip region": "channel", | ||
140 | "dc:setup:flow rate": 0.08, | 140 | "dc:setup:flow rate": 0.08, | ||
141 | "dc:setup:flow rate sample": 0.02, | 141 | "dc:setup:flow rate sample": 0.02, | ||
142 | "dc:setup:flow rate sheath": 0.06, | 142 | "dc:setup:flow rate sheath": 0.06, | ||
143 | "dc:setup:identifier": "RTDC1", | 143 | "dc:setup:identifier": "RTDC1", | ||
144 | "dc:setup:medium": "CellCarrier", | 144 | "dc:setup:medium": "CellCarrier", | ||
145 | "dc:setup:module composition": "AcCellerator", | 145 | "dc:setup:module composition": "AcCellerator", | ||
146 | "dc:setup:software version": "dclab 0.27.8 | dclab 0.27.8", | 146 | "dc:setup:software version": "dclab 0.27.8 | dclab 0.27.8", | ||
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168 | { | 168 | { | ||
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171 | "created": "2020-09-16T16:02:49.868644", | 171 | "created": "2020-09-16T16:02:49.868644", | ||
172 | "dc:experiment:date": "2016-03-03", | 172 | "dc:experiment:date": "2016-03-03", | ||
173 | "dc:experiment:event count": 7560, | 173 | "dc:experiment:event count": 7560, | ||
174 | "dc:experiment:run index": 5, | 174 | "dc:experiment:run index": 5, | ||
175 | "dc:experiment:sample": "S1 beads | 175 | "dc:experiment:sample": "S1 beads | ||
176 | 0.12\u2009\u00b5Ls\u207b\u00b9", | 176 | 0.12\u2009\u00b5Ls\u207b\u00b9", | ||
177 | "dc:experiment:time": "15:06:00", | 177 | "dc:experiment:time": "15:06:00", | ||
178 | "dc:imaging:flash device": "LED (undefined)", | 178 | "dc:imaging:flash device": "LED (undefined)", | ||
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187 | "dc:online_contour:bin kernel": 5, | 187 | "dc:online_contour:bin kernel": 5, | ||
188 | "dc:online_contour:bin threshold": 3, | 188 | "dc:online_contour:bin threshold": 3, | ||
189 | "dc:online_contour:image blur": 0, | 189 | "dc:online_contour:image blur": 0, | ||
190 | "dc:online_contour:no absdiff": true, | 190 | "dc:online_contour:no absdiff": true, | ||
191 | "dc:setup:channel width": 20.0, | 191 | "dc:setup:channel width": 20.0, | ||
192 | "dc:setup:chip region": "channel", | 192 | "dc:setup:chip region": "channel", | ||
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195 | "dc:setup:flow rate sheath": 0.09, | 195 | "dc:setup:flow rate sheath": 0.09, | ||
196 | "dc:setup:identifier": "RTDC1", | 196 | "dc:setup:identifier": "RTDC1", | ||
197 | "dc:setup:medium": "CellCarrier", | 197 | "dc:setup:medium": "CellCarrier", | ||
198 | "dc:setup:module composition": "AcCellerator", | 198 | "dc:setup:module composition": "AcCellerator", | ||
199 | "dc:setup:software version": "dclab 0.27.8 | dclab 0.27.8", | 199 | "dc:setup:software version": "dclab 0.27.8 | dclab 0.27.8", | ||
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