Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_14.04.23 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "4df84ef4065c24d2264ef6e1be5baec5",
"name": "M1_20170606_2021_dsRNA_NK2_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "12:04:47"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9668 (max 9669) in '9668'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.2327
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.94393e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1550 frames Cell Events: 75 mean_circ: 0.94709
Ran for 2050 frames Cell Events: 84 mean_circ: 0.949214
Ran for 2550 frames Cell Events: 103 mean_circ: 0.947828
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Ran for 4050 frames Cell Events: 188 mean_circ: 0.950282
Ran for 4550 frames Cell Events: 217 mean_circ: 0.950483
Ran for 5550 frames Cell Events: 248 mean_circ: 0.950959
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Ran for 6550 frames Cell Events: 283 mean_circ: 0.951765
Ran for 7050 frames Cell Events: 298 mean_circ: 0.95233
Ran for 7550 frames Cell Events: 317 mean_circ: 0.952102
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Ran for 11050 frames Cell Events: 459 mean_circ: 0.94838
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Ran for 15050 frames Cell Events: 658 mean_circ: 0.948483
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Ran for 74050 frames Cell Events: 2983 mean_circ: 0.949043
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Ran for 76550 frames Cell Events: 3067 mean_circ: 0.94911
Ran for 77550 frames Cell Events: 3096 mean_circ: 0.949115
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Ran for 84050 frames Cell Events: 3321 mean_circ: 0.94914
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Ran for 87550 frames Cell Events: 3504 mean_circ: 0.94909
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Ran for 91050 frames Cell Events: 3684 mean_circ: 0.949207
Ran for 91550 frames Cell Events: 3703 mean_circ: 0.949155
Ran for 92050 frames Cell Events: 3728 mean_circ: 0.949182
Ran for 94050 frames Cell Events: 3854 mean_circ: 0.949044
Ran for 94550 frames Cell Events: 3865 mean_circ: 0.949062
Ran for 95550 frames Cell Events: 3892 mean_circ: 0.949073
Ran for 96050 frames Cell Events: 3913 mean_circ: 0.949071
Ran for 96550 frames Cell Events: 3943 mean_circ: 0.949002
Ran for 97550 frames Cell Events: 3998 mean_circ: 0.948961
Ran for 99050 frames Cell Events: 4072 mean_circ: 0.948964
Ran for 99550 frames Cell Events: 4104 mean_circ: 0.948966
Ran for 100050 frames Cell Events: 4119 mean_circ: 0.948983
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Ran for 101050 frames Cell Events: 4144 mean_circ: 0.948968
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Ran for 102050 frames Cell Events: 4188 mean_circ: 0.948999
Ran for 103550 frames Cell Events: 4289 mean_circ: 0.948897
Ran for 104050 frames Cell Events: 4316 mean_circ: 0.948839
Ran for 105050 frames Cell Events: 4347 mean_circ: 0.948743
Ran for 107550 frames Cell Events: 4474 mean_circ: 0.948906
Ran for 108050 frames Cell Events: 4498 mean_circ: 0.94889
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Ran for 112550 frames Cell Events: 4655 mean_circ: 0.948987
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Ran for 116050 frames Cell Events: 4793 mean_circ: 0.94908
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Ran for 118050 frames Cell Events: 4852 mean_circ: 0.949108
Ran for 119550 frames Cell Events: 4911 mean_circ: 0.949096
Ran for 120050 frames Cell Events: 4922 mean_circ: 0.949131
Ran for 121050 frames Cell Events: 4969 mean_circ: 0.949128
Ran for 121550 frames Cell Events: 4997 mean_circ: 0.949106
Ran for 122050 frames Cell Events: 5027 mean_circ: 0.949077
Ran for 122550 frames Cell Events: 5056 mean_circ: 0.949053
Ran for 123050 frames Cell Events: 5071 mean_circ: 0.949088
Ran for 123550 frames Cell Events: 5085 mean_circ: 0.949095
Ran for 124550 frames Cell Events: 5123 mean_circ: 0.949171
Ran for 125050 frames Cell Events: 5134 mean_circ: 0.949164
Ran for 126050 frames Cell Events: 5156 mean_circ: 0.94912
Ran for 127050 frames Cell Events: 5191 mean_circ: 0.949184
Ran for 127550 frames Cell Events: 5202 mean_circ: 0.94912
Ran for 128550 frames Cell Events: 5250 mean_circ: 0.949154
Ran for 131050 frames Cell Events: 5343 mean_circ: 0.949147
Ran for 131550 frames Cell Events: 5360 mean_circ: 0.949123
Ran for 132050 frames Cell Events: 5371 mean_circ: 0.949131
Ran for 132550 frames Cell Events: 5391 mean_circ: 0.949131
Ran for 133550 frames Cell Events: 5434 mean_circ: 0.949086
Ran for 134050 frames Cell Events: 5446 mean_circ: 0.94912
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Ran for 138050 frames Cell Events: 5614 mean_circ: 0.949015
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Ran for 141050 frames Cell Events: 5746 mean_circ: 0.949064
Ran for 142550 frames Cell Events: 5804 mean_circ: 0.949183
Ran for 143050 frames Cell Events: 5824 mean_circ: 0.949196
Ran for 143550 frames Cell Events: 5839 mean_circ: 0.949266
Ran for 144050 frames Cell Events: 5862 mean_circ: 0.94923
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Ran for 146550 frames Cell Events: 5950 mean_circ: 0.949198
Ran for 147550 frames Cell Events: 5991 mean_circ: 0.949192
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Ran for 153050 frames Cell Events: 6215 mean_circ: 0.949306
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Ran for 206550 frames Cell Events: 8642 mean_circ: 0.949245
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Ran for 207550 frames Cell Events: 8688 mean_circ: 0.949182
Ran for 208050 frames Cell Events: 8714 mean_circ: 0.949203
Ran for 209050 frames Cell Events: 8769 mean_circ: 0.949169
Ran for 209550 frames Cell Events: 8805 mean_circ: 0.949137
Ran for 210050 frames Cell Events: 8828 mean_circ: 0.949155
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Ran for 211550 frames Cell Events: 8897 mean_circ: 0.949102
Ran for 212050 frames Cell Events: 8914 mean_circ: 0.949118
Ran for 213050 frames Cell Events: 8955 mean_circ: 0.949098
Ran for 214050 frames Cell Events: 9010 mean_circ: 0.949055
Ran for 214550 frames Cell Events: 9057 mean_circ: 0.94905
Ran for 215050 frames Cell Events: 9074 mean_circ: 0.949018
Ran for 216550 frames Cell Events: 9134 mean_circ: 0.948992
Ran for 218050 frames Cell Events: 9202 mean_circ: 0.948994
Ran for 221550 frames Cell Events: 9348 mean_circ: 0.948986
Ran for 222550 frames Cell Events: 9385 mean_circ: 0.948949
Ran for 223050 frames Cell Events: 9403 mean_circ: 0.948927
Ran for 223550 frames Cell Events: 9425 mean_circ: 0.948919
Ran for 224050 frames Cell Events: 9445 mean_circ: 0.948872
Ran for 224550 frames Cell Events: 9459 mean_circ: 0.948872
Ran for 225050 frames Cell Events: 9480 mean_circ: 0.948873
Ran for 225550 frames Cell Events: 9500 mean_circ: 0.948905
Ran for 226550 frames Cell Events: 9534 mean_circ: 0.948927
Ran for 227050 frames Cell Events: 9553 mean_circ: 0.948953
Ran for 227550 frames Cell Events: 9582 mean_circ: 0.948932
Ran for 228050 frames Cell Events: 9598 mean_circ: 0.948931
Ran for 228550 frames Cell Events: 9614 mean_circ: 0.948924
Ran for 229550 frames Cell Events: 9649 mean_circ: 0.948934



End this measurment..



Stopping time: 1.94405e+009
****************************************
Measurement finished

Mean Circularitiy: 0.948936
Mean Area: 645.454
Mean FL-1 intensity: 3979.72
Mean FL-2 intensity: 4670.35
Mean FL-3 intensity: 0

frames processed: 229614
Total Time: 115013ms
Number of Runs (new bg img) 0
Frames collected: 229900
Skipped frames: 142293
Rescued Frames: 3750026
Samples DAQ: 57475353
Samples written: 114949724
Samples per Channel: 57474862
FPS: 1998.91

Samples per second: 499727
Expected samples/s: 500000

Events: 9669
Events per second: 84.0691
jumped back (PMT): 4505
#Frames read, frequency
# 0 frames :338681
# 1 frames :103143
# 2 frames :4551
# 3 frames :1742
# 4 frames :1096
# 5 frames :1324
# 6 frames :1928
# 7 frames :1408
# 8 frames :888
# 9 frames :632
# 10 frames :588
# 11 frames :589
# 12 frames :557
# 13 frames :437
# 14 frames :354
# 15 frames :240
# 16 frames :239
# 17 frames :227
# 18 frames :211
# 19 frames :144
# 20 frames :110
# 21 frames :81
# 22 frames :63
# 23 frames :60
# 24 frames :69
# 25 frames :43
# 26 frames :45
# 27 frames :26
# 28 frames :39
# 29 frames :29
# 30 frames :32
# 31 frames :19
# 32 frames :19
# 33 frames :25
# 34 frames :13
# 35 frames :12
# 36 frames :7
# 37 frames :6
# 38 frames :7
# 39 frames :8
# 40 frames :4
# 41 frames :5
# 42 frames :2
# 43 frames :2
# 44 frames :2
# 45 frames :2
# 46 frames :5
# 47 frames :4
# 48 frames :1
# 49 frames :2
# 50 frames :1
# 52 frames :1
# 55 frames :2
# 58 frames :1
# 66 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.2327
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0