Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_14.13.42 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "97d96a3a693b77a44471a337c1dd92c8",
"name": "M1_20170608_1306_NK1_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "12:14:06"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9485 (max 9486) in '9485'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.6618
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.09061e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 2050 frames Cell Events: 85 mean_circ: 0.942233
Ran for 2550 frames Cell Events: 103 mean_circ: 0.939579
Ran for 3050 frames Cell Events: 122 mean_circ: 0.936793
Ran for 4550 frames Cell Events: 194 mean_circ: 0.937486
Ran for 5050 frames Cell Events: 215 mean_circ: 0.936649
Ran for 7550 frames Cell Events: 331 mean_circ: 0.938427
Ran for 10050 frames Cell Events: 463 mean_circ: 0.938391
Ran for 10550 frames Cell Events: 483 mean_circ: 0.937971
Ran for 11050 frames Cell Events: 506 mean_circ: 0.937932
Ran for 13050 frames Cell Events: 582 mean_circ: 0.938704
Ran for 13550 frames Cell Events: 598 mean_circ: 0.938741
Ran for 16050 frames Cell Events: 714 mean_circ: 0.938174
Ran for 16550 frames Cell Events: 730 mean_circ: 0.938507
Ran for 18550 frames Cell Events: 837 mean_circ: 0.938792
Ran for 21050 frames Cell Events: 938 mean_circ: 0.939323
Ran for 22050 frames Cell Events: 971 mean_circ: 0.93915
Ran for 24050 frames Cell Events: 1060 mean_circ: 0.939168
Ran for 24550 frames Cell Events: 1087 mean_circ: 0.939202
Ran for 26050 frames Cell Events: 1173 mean_circ: 0.939092
Ran for 26550 frames Cell Events: 1195 mean_circ: 0.939092
Ran for 27050 frames Cell Events: 1220 mean_circ: 0.939224
Ran for 28550 frames Cell Events: 1289 mean_circ: 0.939366
Ran for 29550 frames Cell Events: 1329 mean_circ: 0.939269
Ran for 31050 frames Cell Events: 1390 mean_circ: 0.939386
Ran for 31550 frames Cell Events: 1413 mean_circ: 0.939386
Ran for 32050 frames Cell Events: 1445 mean_circ: 0.939489
Ran for 33050 frames Cell Events: 1481 mean_circ: 0.939605
Ran for 34050 frames Cell Events: 1525 mean_circ: 0.93957
Ran for 36050 frames Cell Events: 1629 mean_circ: 0.939705
Ran for 36550 frames Cell Events: 1647 mean_circ: 0.939694
Ran for 37050 frames Cell Events: 1673 mean_circ: 0.939622
Ran for 39550 frames Cell Events: 1793 mean_circ: 0.939615
Ran for 41050 frames Cell Events: 1860 mean_circ: 0.939371
Ran for 42050 frames Cell Events: 1894 mean_circ: 0.939512
Ran for 42550 frames Cell Events: 1910 mean_circ: 0.939512
Ran for 45550 frames Cell Events: 2029 mean_circ: 0.939286
Ran for 46050 frames Cell Events: 2053 mean_circ: 0.939246
Ran for 47050 frames Cell Events: 2086 mean_circ: 0.939477
Ran for 48550 frames Cell Events: 2157 mean_circ: 0.939417
Ran for 50050 frames Cell Events: 2234 mean_circ: 0.939577
Ran for 51050 frames Cell Events: 2285 mean_circ: 0.939288
Ran for 53550 frames Cell Events: 2417 mean_circ: 0.93926
Ran for 54550 frames Cell Events: 2468 mean_circ: 0.939295
Ran for 56050 frames Cell Events: 2543 mean_circ: 0.939505
Ran for 56550 frames Cell Events: 2570 mean_circ: 0.939535
Ran for 59050 frames Cell Events: 2699 mean_circ: 0.939602
Ran for 61050 frames Cell Events: 2789 mean_circ: 0.939639
Ran for 61550 frames Cell Events: 2810 mean_circ: 0.93957
Ran for 62050 frames Cell Events: 2822 mean_circ: 0.939555
Ran for 62550 frames Cell Events: 2837 mean_circ: 0.939536
Ran for 63050 frames Cell Events: 2856 mean_circ: 0.939581
Ran for 64050 frames Cell Events: 2899 mean_circ: 0.939685
Ran for 65050 frames Cell Events: 2942 mean_circ: 0.939676
Ran for 65550 frames Cell Events: 2959 mean_circ: 0.939698
Ran for 66050 frames Cell Events: 2989 mean_circ: 0.939687
Ran for 67550 frames Cell Events: 3071 mean_circ: 0.939691
Ran for 68550 frames Cell Events: 3119 mean_circ: 0.939784
Ran for 69050 frames Cell Events: 3139 mean_circ: 0.939894
Ran for 69550 frames Cell Events: 3161 mean_circ: 0.939861
Ran for 70550 frames Cell Events: 3200 mean_circ: 0.939858
Ran for 73050 frames Cell Events: 3327 mean_circ: 0.939918
Ran for 74550 frames Cell Events: 3408 mean_circ: 0.939927
Ran for 76050 frames Cell Events: 3477 mean_circ: 0.93994
Ran for 77550 frames Cell Events: 3541 mean_circ: 0.940041
Ran for 78050 frames Cell Events: 3567 mean_circ: 0.940005
Ran for 79050 frames Cell Events: 3633 mean_circ: 0.940052
Ran for 79550 frames Cell Events: 3647 mean_circ: 0.940039
Ran for 81050 frames Cell Events: 3716 mean_circ: 0.939863
Ran for 81550 frames Cell Events: 3738 mean_circ: 0.939852
Ran for 82050 frames Cell Events: 3767 mean_circ: 0.939907
Ran for 85550 frames Cell Events: 3917 mean_circ: 0.939806
Ran for 87550 frames Cell Events: 4007 mean_circ: 0.939825
Ran for 88050 frames Cell Events: 4033 mean_circ: 0.939855
Ran for 88550 frames Cell Events: 4053 mean_circ: 0.939872
Ran for 91050 frames Cell Events: 4157 mean_circ: 0.939959
Ran for 91550 frames Cell Events: 4183 mean_circ: 0.939981
Ran for 92050 frames Cell Events: 4195 mean_circ: 0.940009
Ran for 93050 frames Cell Events: 4229 mean_circ: 0.940105
Ran for 93550 frames Cell Events: 4251 mean_circ: 0.940133
Ran for 94050 frames Cell Events: 4273 mean_circ: 0.940161
Ran for 95050 frames Cell Events: 4318 mean_circ: 0.940186
Ran for 96550 frames Cell Events: 4377 mean_circ: 0.940235
Ran for 98550 frames Cell Events: 4478 mean_circ: 0.940295
Ran for 100050 frames Cell Events: 4548 mean_circ: 0.940332
Ran for 101050 frames Cell Events: 4592 mean_circ: 0.940262
Ran for 102050 frames Cell Events: 4633 mean_circ: 0.94033
Ran for 104050 frames Cell Events: 4720 mean_circ: 0.940325
Ran for 105550 frames Cell Events: 4790 mean_circ: 0.940399
Ran for 107550 frames Cell Events: 4886 mean_circ: 0.940314
Ran for 108050 frames Cell Events: 4903 mean_circ: 0.940331
Ran for 110050 frames Cell Events: 4986 mean_circ: 0.940347
Ran for 110550 frames Cell Events: 5003 mean_circ: 0.940368
Ran for 113550 frames Cell Events: 5130 mean_circ: 0.940547
Ran for 115050 frames Cell Events: 5212 mean_circ: 0.940532
Ran for 118050 frames Cell Events: 5359 mean_circ: 0.940472
Ran for 119050 frames Cell Events: 5406 mean_circ: 0.94043
Ran for 119550 frames Cell Events: 5429 mean_circ: 0.940398
Ran for 120050 frames Cell Events: 5458 mean_circ: 0.9404
Ran for 121550 frames Cell Events: 5520 mean_circ: 0.940414
Ran for 125050 frames Cell Events: 5682 mean_circ: 0.940213
Ran for 126550 frames Cell Events: 5744 mean_circ: 0.940247
Ran for 127050 frames Cell Events: 5772 mean_circ: 0.940277
Ran for 128550 frames Cell Events: 5837 mean_circ: 0.940368
Ran for 129550 frames Cell Events: 5873 mean_circ: 0.940338
Ran for 130050 frames Cell Events: 5896 mean_circ: 0.940384
Ran for 130550 frames Cell Events: 5906 mean_circ: 0.940393
Ran for 131050 frames Cell Events: 5929 mean_circ: 0.940446
Ran for 132050 frames Cell Events: 5982 mean_circ: 0.940495
Ran for 133050 frames Cell Events: 6026 mean_circ: 0.940541
Ran for 134050 frames Cell Events: 6066 mean_circ: 0.940541
Ran for 135550 frames Cell Events: 6117 mean_circ: 0.940579
Ran for 136050 frames Cell Events: 6145 mean_circ: 0.940585
Ran for 137550 frames Cell Events: 6207 mean_circ: 0.940573
Ran for 141550 frames Cell Events: 6386 mean_circ: 0.940673
Ran for 142050 frames Cell Events: 6405 mean_circ: 0.940687
Ran for 145550 frames Cell Events: 6563 mean_circ: 0.940644
Ran for 147050 frames Cell Events: 6620 mean_circ: 0.940631
Ran for 147550 frames Cell Events: 6657 mean_circ: 0.940631
Ran for 150050 frames Cell Events: 6775 mean_circ: 0.940732
Ran for 151550 frames Cell Events: 6839 mean_circ: 0.940739
Ran for 153050 frames Cell Events: 6904 mean_circ: 0.940746
Ran for 154550 frames Cell Events: 6983 mean_circ: 0.940725
Ran for 155050 frames Cell Events: 7004 mean_circ: 0.94072
Ran for 156050 frames Cell Events: 7039 mean_circ: 0.940747
Ran for 156550 frames Cell Events: 7052 mean_circ: 0.940732
Ran for 158550 frames Cell Events: 7135 mean_circ: 0.940796
Ran for 160050 frames Cell Events: 7223 mean_circ: 0.940877
Ran for 160550 frames Cell Events: 7256 mean_circ: 0.940967
Ran for 161050 frames Cell Events: 7284 mean_circ: 0.940972
Ran for 161550 frames Cell Events: 7311 mean_circ: 0.940964
Ran for 162050 frames Cell Events: 7337 mean_circ: 0.940974
Ran for 163050 frames Cell Events: 7373 mean_circ: 0.941005
Ran for 164050 frames Cell Events: 7415 mean_circ: 0.940966
Ran for 166050 frames Cell Events: 7513 mean_circ: 0.940999
Ran for 166550 frames Cell Events: 7536 mean_circ: 0.940996
Ran for 170050 frames Cell Events: 7681 mean_circ: 0.941069
Ran for 171050 frames Cell Events: 7728 mean_circ: 0.941021
Ran for 171550 frames Cell Events: 7741 mean_circ: 0.941023
Ran for 175550 frames Cell Events: 7893 mean_circ: 0.941099
Ran for 176050 frames Cell Events: 7909 mean_circ: 0.941075
Ran for 176550 frames Cell Events: 7934 mean_circ: 0.941115
Ran for 178550 frames Cell Events: 8036 mean_circ: 0.941199
Ran for 179050 frames Cell Events: 8055 mean_circ: 0.941211
Ran for 179550 frames Cell Events: 8077 mean_circ: 0.941228
Ran for 180050 frames Cell Events: 8100 mean_circ: 0.941242
Ran for 180550 frames Cell Events: 8118 mean_circ: 0.941236
Ran for 181050 frames Cell Events: 8140 mean_circ: 0.941243
Ran for 181550 frames Cell Events: 8160 mean_circ: 0.941257
Ran for 185550 frames Cell Events: 8336 mean_circ: 0.94121
Ran for 186050 frames Cell Events: 8352 mean_circ: 0.941224
Ran for 189050 frames Cell Events: 8490 mean_circ: 0.941312
Ran for 192050 frames Cell Events: 8621 mean_circ: 0.941336
Ran for 192550 frames Cell Events: 8644 mean_circ: 0.941336
Ran for 193050 frames Cell Events: 8664 mean_circ: 0.94132
Ran for 194550 frames Cell Events: 8731 mean_circ: 0.941306
Ran for 195050 frames Cell Events: 8750 mean_circ: 0.941314
Ran for 196050 frames Cell Events: 8793 mean_circ: 0.941374
Ran for 199550 frames Cell Events: 8939 mean_circ: 0.941383
Ran for 200550 frames Cell Events: 8978 mean_circ: 0.941409
Ran for 202050 frames Cell Events: 9032 mean_circ: 0.941436
Ran for 202550 frames Cell Events: 9049 mean_circ: 0.941412
Ran for 207550 frames Cell Events: 9320 mean_circ: 0.941447
Ran for 208550 frames Cell Events: 9364 mean_circ: 0.941411
Ran for 209550 frames Cell Events: 9402 mean_circ: 0.941416
Ran for 210050 frames Cell Events: 9416 mean_circ: 0.941414
Ran for 211550 frames Cell Events: 9473 mean_circ: 0.941415



End this measurment..



Stopping time: 2.09072e+009
****************************************
Measurement finished

Mean Circularitiy: 0.941403
Mean Area: 637.611
Mean FL-1 intensity: 4194.29
Mean FL-2 intensity: 5130.96
Mean FL-3 intensity: 0

frames processed: 206781
Total Time: 106030ms
Number of Runs (new bg img) 0
Frames collected: 211891
Skipped frames: 5110
Rescued Frames: 128688
Samples DAQ: 52975799
Samples written: 105945136
Samples per Channel: 52972568
FPS: 1998.4

Samples per second: 499599
Expected samples/s: 500000

Events: 9486
Events per second: 89.465
jumped back (PMT): 3267
#Frames read, frequency
# 0 frames :345393
# 1 frames :62889
# 2 frames :2982
# 3 frames :1464
# 4 frames :918
# 5 frames :609
# 6 frames :412
# 7 frames :1226
# 8 frames :1025
# 9 frames :863
# 10 frames :731
# 11 frames :676
# 12 frames :570
# 13 frames :441
# 14 frames :340
# 15 frames :270
# 16 frames :226
# 17 frames :210
# 18 frames :280
# 19 frames :258
# 20 frames :216
# 21 frames :185
# 22 frames :159
# 23 frames :145
# 24 frames :118
# 25 frames :68
# 26 frames :80
# 27 frames :82
# 28 frames :63
# 29 frames :85
# 30 frames :49
# 31 frames :63
# 32 frames :48
# 33 frames :31
# 34 frames :30
# 35 frames :31
# 36 frames :36
# 37 frames :14
# 38 frames :23
# 39 frames :23
# 40 frames :26
# 41 frames :17
# 42 frames :21
# 43 frames :17
# 44 frames :11
# 45 frames :11
# 46 frames :10
# 47 frames :9
# 48 frames :14
# 49 frames :8
# 50 frames :10
# 51 frames :13
# 52 frames :3
# 53 frames :2
# 54 frames :7
# 55 frames :3
# 56 frames :4
# 57 frames :10
# 58 frames :4
# 59 frames :1
# 60 frames :5
# 61 frames :6
# 62 frames :3
# 63 frames :5
# 64 frames :1
# 65 frames :2
# 66 frames :1
# 67 frames :2
# 69 frames :2
# 72 frames :1
# 73 frames :3
# 75 frames :2
# 77 frames :1
# 79 frames :2
# 86 frames :1
# 96 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.6618
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0