Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_18.17.32 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "44ddd315d365922027bbf75e705fd882",
"name": "M1_20170518_2102_NK3_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "16:17:57"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 7992 (max 7993) in '7992'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 27.5087
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.04813e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 28 mean_circ: 0.951355
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Ran for 3050 frames Cell Events: 84 mean_circ: 0.950343
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Ran for 91550 frames Cell Events: 3045 mean_circ: 0.940729
Ran for 92050 frames Cell Events: 3065 mean_circ: 0.940714
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Ran for 96050 frames Cell Events: 3194 mean_circ: 0.940665
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Ran for 107050 frames Cell Events: 3599 mean_circ: 0.940391
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Ran for 123050 frames Cell Events: 4167 mean_circ: 0.939907
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Ran for 124550 frames Cell Events: 4219 mean_circ: 0.940052
Ran for 125050 frames Cell Events: 4235 mean_circ: 0.940045
Ran for 126050 frames Cell Events: 4266 mean_circ: 0.940073
Ran for 126550 frames Cell Events: 4276 mean_circ: 0.940091
Ran for 127550 frames Cell Events: 4317 mean_circ: 0.940111
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Ran for 195550 frames Cell Events: 6900 mean_circ: 0.939492
Ran for 196050 frames Cell Events: 6916 mean_circ: 0.939457
Ran for 196550 frames Cell Events: 6927 mean_circ: 0.939449
Ran for 197550 frames Cell Events: 6964 mean_circ: 0.939395
Ran for 198550 frames Cell Events: 7014 mean_circ: 0.939402
Ran for 199050 frames Cell Events: 7032 mean_circ: 0.939412
Ran for 200050 frames Cell Events: 7071 mean_circ: 0.939356
Ran for 200550 frames Cell Events: 7088 mean_circ: 0.939352
Ran for 201050 frames Cell Events: 7119 mean_circ: 0.939352
Ran for 201550 frames Cell Events: 7131 mean_circ: 0.939367
Ran for 202050 frames Cell Events: 7163 mean_circ: 0.939403
Ran for 202550 frames Cell Events: 7184 mean_circ: 0.939438
Ran for 203550 frames Cell Events: 7231 mean_circ: 0.939357
Ran for 204550 frames Cell Events: 7276 mean_circ: 0.93939
Ran for 205550 frames Cell Events: 7316 mean_circ: 0.939411
Ran for 206050 frames Cell Events: 7338 mean_circ: 0.939372
Ran for 206550 frames Cell Events: 7360 mean_circ: 0.939363
Ran for 208550 frames Cell Events: 7423 mean_circ: 0.939423
Ran for 209050 frames Cell Events: 7450 mean_circ: 0.939455
Ran for 209550 frames Cell Events: 7463 mean_circ: 0.939425
Ran for 211050 frames Cell Events: 7497 mean_circ: 0.939421
Ran for 211550 frames Cell Events: 7517 mean_circ: 0.939427
Ran for 212050 frames Cell Events: 7535 mean_circ: 0.93944
Ran for 213550 frames Cell Events: 7593 mean_circ: 0.939472
Ran for 214050 frames Cell Events: 7608 mean_circ: 0.939478
Ran for 214550 frames Cell Events: 7627 mean_circ: 0.93948
Ran for 215550 frames Cell Events: 7657 mean_circ: 0.939469
Ran for 216050 frames Cell Events: 7679 mean_circ: 0.939495
Ran for 216550 frames Cell Events: 7694 mean_circ: 0.93946
Ran for 217550 frames Cell Events: 7718 mean_circ: 0.939445
Ran for 218050 frames Cell Events: 7741 mean_circ: 0.939445
Ran for 218550 frames Cell Events: 7767 mean_circ: 0.939456
Ran for 219550 frames Cell Events: 7823 mean_circ: 0.939488
Ran for 220050 frames Cell Events: 7849 mean_circ: 0.939432
Ran for 220550 frames Cell Events: 7865 mean_circ: 0.93948
Ran for 221050 frames Cell Events: 7878 mean_circ: 0.939501
Ran for 221550 frames Cell Events: 7900 mean_circ: 0.939509
Ran for 223050 frames Cell Events: 7963 mean_circ: 0.939551
Ran for 223550 frames Cell Events: 7986 mean_circ: 0.939554



End this measurment..



Stopping time: 3.04925e+008
****************************************
Measurement finished

Mean Circularitiy: 0.93954
Mean Area: 698.929
Mean FL-1 intensity: 2976.8
Mean FL-2 intensity: 4916.1
Mean FL-3 intensity: 0

frames processed: 222894
Total Time: 111982ms
Number of Runs (new bg img) 0
Frames collected: 223845
Skipped frames: 100768
Rescued Frames: 5489586
Samples DAQ: 55961314
Samples written: 111922236
Samples per Channel: 55961118
FPS: 1998.93

Samples per second: 499732
Expected samples/s: 500000

Events: 7993
Events per second: 71.3774
jumped back (PMT): 4818
#Frames read, frequency
# 0 frames :313442
# 1 frames :117888
# 2 frames :4651
# 3 frames :1610
# 4 frames :889
# 5 frames :365
# 6 frames :1243
# 7 frames :2445
# 8 frames :1079
# 9 frames :657
# 10 frames :981
# 11 frames :497
# 12 frames :396
# 13 frames :288
# 14 frames :178
# 15 frames :198
# 16 frames :137
# 17 frames :136
# 18 frames :91
# 19 frames :74
# 20 frames :58
# 21 frames :48
# 22 frames :44
# 23 frames :40
# 24 frames :37
# 25 frames :20
# 26 frames :20
# 27 frames :22
# 28 frames :14
# 29 frames :20
# 30 frames :11
# 31 frames :11
# 32 frames :10
# 33 frames :5
# 34 frames :7
# 35 frames :4
# 36 frames :2
# 37 frames :1
# 38 frames :1
# 40 frames :2
# 41 frames :2
# 42 frames :2
# 44 frames :1
# 45 frames :1
# 46 frames :1
# 54 frames :1
# 56 frames :1
# 61 frames :1
# 64 frames :1
# 68 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 27.5087
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0