DCOR

dclab-tdms2rtdc

{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "3afc201485e2f34f2a671cf632bf2388",
"name": "M3_data.tdms"
}
],
"libraries": {
"dclab": "0.57.4",
"h5py": "3.10.0",
"imageio": "2.10.3",
"nptdms": "1.8.0",
"numpy": "1.26.3"
},
"python": {
"build": "tags/v3.11.6:8b6ee5b, Oct 2 2023 14:57:12",
"implementation": "CPython",
"version": "3.11.6"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-02-21",
"time": "21:48:58"
}
}

dclab-tdms2rtdc-warnings

MultipleSamplesPerEventFound
in dclab.rtdc_dataset.fmt_tdms.exc line 101:
Ignoring trace data of
'\\syn03.frontend.storage.int.mpl.mpg.de\guck_division2\Members\Laura\20240219_Kasia_screen_all_folders\20180731_Conny_Kinases_Set1\CONTROL\M3_data_traces.tdms'
due to multiple values for samples per event: [ 424 425 426 5526 6800 7226 7650 7652
8074 8076 8500 8926 9348 9350 9352 9774 9776 10200 10624 10626 11474 11476 11900 12324
12326 12750 12752 13176 13600 14026 14450 14452 14874 14876 15300 15724 15726 16574 17000 17424
17426 17850 18274 19550 19552 19976 20824 20826 21248 21676 22100 23374 29326]

M3_camera.ini

[Framerate]
Shutter Time = 20
Frame Rate = 1250
Tap Mode = 10x8 Bit
Tap Code = 6

[ROI]
x-pos = 629
y-pos = 512
width = 250
height = 80

M3_events.txt

[EVENT LOG]

M3_para.ini

[General]
Software Version = ShapeIn 2.0.4
Measurement Number = 3
Shutter Time LED [us] = 2
Sample Name = CONTROL
Flow Rate [ul/s] = 0.04
Sheath Flow Rate [ul/s] = 0.03
Sample Flow Rate [ul/s] = 0.01
Region = "Channel"
Channel width [um] = 20
Video Frame Offset = 0
Buffer Medium = CellCarrier
Date [YYYY-MM-DD] = 2018-07-31
Start Time [hh:mm:ss] = 15:27:14

[Image]
Pix Size = 0.34
Thresh = -6
Trig Thresh = 10

[Gating]
Min Length= 3
Max Length= 80
Min y= 3
Max y= 80

[FLUOR]
Samplerate [sps] = 500000
Bitdepthraw = 16
Format = int16
ADCmin [V] = -1
ADCmax [V] = 1
FL-daqCode = 3
Laser1 488 nm Power [%] = 32
Laser2 561 nm Power [%] = 100

M3_SoftwareSettings.ini

[Gating]
Stop_N_Events_ON=true
Stop_N_Events=10000
Stop_Time_ON=false
Stop_Time=15
Object_MinLength=3
Object_MaxLength=80
Object_Length_soft_ON=false
Object_Length_ON=true
Object_MinHeight=3
Object_MaxHeigth=80
Object_Height_soft_ON=false
Object_Height_ON=true
Object_MinAspect=1
Object_MaxAspect=2
Object_Aspect_soft_ON=false
Object_Aspect_ON=false
Object_MinArea=18
Object_MaxArea=1500
Object_Area_soft_ON=false
Object_Area_ON=false
Object_MinDefo=0
Object_MaxDefo=0.2
Object_Defo_soft_ON=false
Object_Defo_ON=false
Object_MinPoro=1
Object_MaxPoro=1.05
Object_Poro_soft_ON=false
Object_Poro_ON=false
FL1_MinIntens=500
FL1_MaxIntens=33000
FL1_soft_ON=true
FL1_ON=false
FL2_MinIntens=500
FL2_MaxIntens=33000
FL2_soft_ON=true
FL2_ON=false
FL3_MinIntens=0
FL3_MaxIntens=33000
FL3_soft_ON=false
FL3_ON=false

[Pump]
Sample_Syringe_Volume=1000
Sample_Syringe_Lenght=57
Sheath_Syringe_Volume=1000
Sheath_Syringe_Lenght=57
Flic_FillRate=0.1
Tube_FillRate=1
Take_Up_Rate=-2
SheathToSample_Ratio=3
automatic_FlowRate=false
standard_FlowRate1=0.04
standard_FlowRate2=0.06
standard_FlowRate3=0.08

[Detection]
Gray_Threshold=6
Pixel_Threshold=10

[ROI]
use_manual_ROI=false
Width=250
Height=80
fps=1250

[SampleProp]
Channel_Size=20
Channel_Size_ID=1
Buffer_Medium_ID=0

[Project]
Last_Exp_Folder=C:\\Users\\Public\\Experiments\\20180731_Conny_Kinases_Set1\\CONTROL

[Measurement]
Measurement_Type_ID=0

[Fluorescence]
Laser1_On=true
Laser2_On=true
Laser3_On=false
Detector1_On=true
Detector2_On=true
Detector3_On=false
Laser1_Power=32
Laser2_Power=100
Laser3_Power=0

[Maintenance]
Pixel_Resolution=0.34
NI_fg_name=img0
numOfChanInstalled=3
numOfLasInstalled=3
comLaser1=COM13
comLaser2=COM12
comLaser3=COM11
FL1dev=pci/ai4
FL2dev=pci/ai5
FL3dev=pci/ai6
Trigger_name=pci/ctr0
AI_StartTrigger_name=/pci/ai/StartTrigger
Interlock_name=pci/port1/line1
Watchdog_name=pci
WatchdogDO_name=pci/port0/line7
Laser1dev=
Laser2dev=
Laser3dev=
invert_pmt=1
TotalAvailableSamplerate=1000000
median_pmt=1
FL1_name=525/50
FL2_name=593/46
FL3_name=700/75
Laser1_name=488 nm
Laser2_name=561 nm
Laser3_name=640 nm
VelocityFactor=3
x_pos=629
y_pos=512

[MaintenanceD]
Version=ShapeIn 2.0.4
x_pos_ff=640
y_pos_ff=512
x_size_ff=1280
y_size_ff=1024
threshold1=180
fps_ff=25
Vmin_AI=-1
Vmax_AI=1
rollingAverage=100
mwPlotUpdateRate=40
mwPumpUpdateRate=100
MinContourLength=8
bin_ops=5
blur=0
DefaultFileVersion=3
blood_relax=0