Figure4d_Blood_Initial.rtdc
| Date of measurement | 2019-06-24 |
|---|---|
| Index of measurement run | 1 |
| Sample name | Figure4d_Blood_Initial |
| Number of recorded events | 3.578e+04 |
| Start time of measurement | 18:28:52 |
| Software | ShapeIn 2.0.8 |
|---|---|
| Modules used | AcCellerator, FluorescenceModule |
| Flow rate in channel | 0.04 µL/s |
| Imaged chip region | channel |
| Medium used | CellCarrier B |
| Sample flow rate | 0.01 µL/s |
| Sheath flow rate | 0.03 µL/s |
| Unique setup identifier | ZMDD-AcC-fe3cdd-2a2250 |
| Width of microfluidic channel | 20 µm |
| Image height | 80 px |
|---|---|
| Image width | 250 px |
| Image x coordinate on sensor | 629 px |
| Image y coordinate on sensor | 512 px |
| Imaging frame rate | 1250 Hz |
| Light source device type | LED (ZMD L1) |
| Light source flash duration | 2 µs |
| Pixel size | 0.34 µm |
| FL1 description | 525/50 |
|---|---|
| FL2 description | 593/46 |
| FL3 description | 700/75 |
| Laser 2 output power | 5 % |
| Laser 2 wavelength | 561 nm |
| Laser 3 output power | 13 % |
| Laser 3 wavelength | 640 nm |
| Lower voltage detection limit | -1 V |
| Number of active channels | 3 |
| Number of active lasers | 2 |
| Number of available channels | 3 |
| Number of available lasers | 3 |
| Rolling median filter size for traces | 3 |
| Samples per event | 265 |
| Trace bit depth | 16 |
| Trace sample rate | 3.125e+05 Hz |
| Upper voltage detection limit | 1 V |
Additional Information
| Field | Value |
|---|---|
| license | Creative Commons Public Domain Dedication |
| created | 3 years ago |
| format | RT-FDC |
| id | f61ba870-4c28-5fce-6ff1-36522278c897 |
| metadata modified | 2 months ago |
| mimetype | RT-DC |
| package id | e61bb335-3523-e539-78d1-0720ad5a99f1 |
| position | 17 |
| s3 available | True |
| s3 url | https://objectstore.hpccloud.mpcdf.mpg.de/circle-5a7a053d-55fb-4f99-960c-f478d0bd418f/resource/f61/ba8/70-4c28-5fce-6ff1-36522278c897 |
| sha256 | be9ec08697f7dedc486f1fe24a3fb483742189b714a133567a31c409929b0362 |
| size | 800.1 MiB |
| dc:experiment:date | 2019-06-24 |
| dc:experiment:event count | 35,776 |
| dc:experiment:run index | 1 |
| dc:experiment:sample | Figure4d_Blood_Initial |
| dc:experiment:time | 18:28:52 |
| dc:fluorescence:bit depth | 16 |
| dc:fluorescence:channel 1 name | 525/50 |
| dc:fluorescence:channel 2 name | 593/46 |
| dc:fluorescence:channel 3 name | 700/75 |
| dc:fluorescence:channel count | 3 |
| dc:fluorescence:channels installed | 3 |
| dc:fluorescence:laser 2 lambda | 561 |
| dc:fluorescence:laser 2 power | 5 |
| dc:fluorescence:laser 3 lambda | 640 |
| dc:fluorescence:laser 3 power | 13 |
| dc:fluorescence:laser count | 2 |
| dc:fluorescence:lasers installed | 3 |
| dc:fluorescence:sample rate | 312,500 |
| dc:fluorescence:samples per event | 265 |
| dc:fluorescence:signal max | 1 |
| dc:fluorescence:signal min | -1 |
| dc:fluorescence:trace median | 3 |
| dc:imaging:flash device | LED (ZMD L1) |
| dc:imaging:flash duration | 2 |
| dc:imaging:frame rate | 1,250 |
| dc:imaging:pixel size | 0.34 |
| dc:imaging:roi position x | 629 |
| dc:imaging:roi position y | 512 |
| dc:imaging:roi size x | 250 |
| dc:imaging:roi size y | 80 |
| dc:online contour:bin area min | 10 |
| dc:online contour:bin kernel | 5 |
| dc:online contour:bin threshold | -6 |
| dc:online contour:image blur | 0 |
| dc:online contour:no absdiff | True |
| dc:online filter:size x max | 80 |
| dc:online filter:size x min | 1 |
| dc:online filter:size x soft limit | False |
| dc:online filter:size y max | 80 |
| dc:online filter:size y min | 1 |
| dc:online filter:size y soft limit | False |
| dc:online filter:target event count | 60,000 |
| dc:setup:channel width | 20 |
| dc:setup:chip region | channel |
| dc:setup:flow rate | 0.04 |
| dc:setup:flow rate sample | 0.01 |
| dc:setup:flow rate sheath | 0.03 |
| dc:setup:identifier | ZMDD-AcC-fe3cdd-2a2250 |
| dc:setup:medium | CellCarrierB |
| dc:setup:module composition | AcCellerator,FluorescenceModule |
| dc:setup:software version | ShapeIn 2.0.8 |