Experiment
Date of measurement 2019-06-14
Index of measurement run 2
Sample name Thymus TG
Number of recorded events 4.892e+04
Start time of measurement 18:39:08

Setup
Software ShapeIn 2.0.8 | dclab 0.38.2 | dclab 0.47.2
Modules used AcCellerator, FluorescenceModule
Flow rate in channel 0.12 µL/s
Imaged chip region channel
Medium used 0.59% MC-PBS
Sample flow rate 0.03 µL/s
Sheath flow rate 0.09 µL/s
Unique setup identifier ZMDD-AcC-47be2b-bd1d61
Width of microfluidic channel 30 µm

Imaging
Image height 100 px
Image width 250 px
Image x coordinate on sensor 605 px
Image y coordinate on sensor 580 px
Imaging frame rate 1600 Hz
Light source device type LED (ZMD L1)
Light source flash duration 2 µs
Pixel size 0.339 µm

Fluorescence
FL1 description 525/50 Green
FL2 description 593/46 Yellow
FL3 description 700/75 Red
Laser 1 output power 30 %
Laser 1 wavelength 488 nm
Laser 2 output power 30 %
Laser 2 wavelength 561 nm
Laser 3 output power 30 %
Laser 3 wavelength 640 nm
Lower voltage detection limit -1 V
Number of active channels 3
Number of active lasers 3
Number of available channels 3
Number of available lasers 3
Rolling median filter size for traces 5
Samples per event 397
Trace bit depth 16
Trace sample rate 6.25e+05 Hz
Upper voltage detection limit 1 V

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