Lung_Enzymatic_RUN1.rtdc
| Date of measurement | 2022-04-28 |
|---|---|
| Index of measurement run | 1 |
| Sample name | lung_1_digested_unstained |
| Number of recorded events | 9.561e+04 |
| Start time of measurement | 13:46:23 |
| Software | 2.3.3.54 | dclab 0.47.2 |
|---|---|
| Modules used | AcCellerator, FluorescenceModule |
| Flow rate in channel | 0.12 µL/s |
| Imaged chip region | channel |
| Mean chip temperature | 25.9 °C |
| Medium used | CellCarrier B |
| Sample flow rate | 0.03 µL/s |
| Sheath flow rate | 0.09 µL/s |
| Unique setup identifier | ZMDD-AcC-774f84-3da488 |
| Width of microfluidic channel | 30 µm |
| Image height | 100 px |
|---|---|
| Image width | 250 px |
| Image x coordinate on sensor | 533 px |
| Image y coordinate on sensor | 470 px |
| Imaging frame rate | 1600 Hz |
| Light source device type | LED (ZMD L1) |
| Light source flash duration | 2 µs |
| Pixel size | 0.339 µm |
| Baseline offset channel 2 | -22 |
|---|---|
| Baseline offset channel 3 | -4 |
| FL2 description | 593/46 |
| FL3 description | 700/75 |
| Laser 2 output power | 2 % |
| Laser 2 wavelength | 561 nm |
| Lower voltage detection limit | -1 V |
| Number of active channels | 2 |
| Number of active lasers | 1 |
| Number of available channels | 3 |
| Number of available lasers | 3 |
| Rolling median filter size for traces | 7 |
| Samples per event | 635 |
| Trace bit depth | 16 |
| Trace sample rate | 1e+06 Hz |
| Upper voltage detection limit | 1 V |
Additional Information
| Field | Value |
|---|---|
| license | Creative Commons Public Domain Dedication |
| created | 1 year ago |
| format | RT-FDC |
| id | ea61eeec-3d58-4a37-8825-3a576ed86e15 |
| metadata modified | 7 months ago |
| mimetype | RT-DC |
| package id | 03c49f24-8d41-445b-aae3-a18790bfe82e |
| position | 11 |
| s3 available | True |
| s3 url | https://objectstore.hpccloud.mpcdf.mpg.de/circle-13748aef-a5b5-47b4-b86d-8a0c1ff49035/resource/ea6/1ee/ec-3d58-4a37-8825-3a576ed86e15 |
| sha256 | dbac9f9a120938bc15b301a4a4b56a1033699e7b25137691f4b1314339aed441 |
| size | 1.3 GiB |
| dc:experiment:date | 2022-04-28 |
| dc:experiment:event count | 95,610 |
| dc:experiment:run index | 1 |
| dc:experiment:sample | lung_1_digested_unstained |
| dc:experiment:time | 13:46:23 |
| dc:fluorescence:baseline 2 offset | -22 |
| dc:fluorescence:baseline 3 offset | -4 |
| dc:fluorescence:bit depth | 16 |
| dc:fluorescence:channel 2 name | 593/46 |
| dc:fluorescence:channel 3 name | 700/75 |
| dc:fluorescence:channel count | 2 |
| dc:fluorescence:channels installed | 3 |
| dc:fluorescence:laser 2 lambda | 561 |
| dc:fluorescence:laser 2 power | 2 |
| dc:fluorescence:laser count | 1 |
| dc:fluorescence:lasers installed | 3 |
| dc:fluorescence:sample rate | 1,000,000 |
| dc:fluorescence:samples per event | 635 |
| dc:fluorescence:signal max | 1 |
| dc:fluorescence:signal min | -1 |
| dc:fluorescence:trace median | 7 |
| dc:imaging:flash device | LED (ZMD L1) |
| dc:imaging:flash duration | 2 |
| dc:imaging:frame rate | 1,600 |
| dc:imaging:pixel size | 0.339 |
| dc:imaging:roi position x | 533 |
| dc:imaging:roi position y | 470 |
| dc:imaging:roi size x | 250 |
| dc:imaging:roi size y | 100 |
| dc:online contour:bg empty | True |
| dc:online contour:bin area min | 10 |
| dc:online contour:bin kernel | 5 |
| dc:online contour:bin threshold | -6 |
| dc:online contour:image blur | 0 |
| dc:online contour:no absdiff | True |
| dc:setup:channel width | 30 |
| dc:setup:chip region | channel |
| dc:setup:flow rate | 0.12 |
| dc:setup:flow rate sample | 0.03 |
| dc:setup:flow rate sheath | 0.09 |
| dc:setup:identifier | ZMDD-AcC-774f84-3da488 |
| dc:setup:medium | CellCarrierB |
| dc:setup:module composition | AcCellerator,FluorescenceModule |
| dc:setup:software version | 2.3.3.54 | dclab 0.47.2 |
| dc:setup:temperature | 25.9 |