Figure1d_01_Beads_FL_Initial.rtdc
| Date of measurement | 2019-05-11 |
|---|---|
| Index of measurement run | 1 |
| Sample name | Figure1d_01_Beads_FL_Initial |
| Number of recorded events | 5.045e+04 |
| Start time of measurement | 19:29:59 |
| Software | ShapeIn 2.0.7 |
|---|---|
| Modules used | AcCellerator, FluorescenceModule |
| Flow rate in channel | 0.04 µL/s |
| Imaged chip region | channel |
| Medium used | 0.59% MC-PBS |
| Sample flow rate | 0.01 µL/s |
| Sheath flow rate | 0.03 µL/s |
| Unique setup identifier | ZMDD-AcC-fe3cdd-2a2250 |
| Width of microfluidic channel | 20 µm |
| Image height | 40 px |
|---|---|
| Image width | 100 px |
| Image x coordinate on sensor | 629 px |
| Image y coordinate on sensor | 512 px |
| Imaging frame rate | 2500 Hz |
| Light source device type | LED (ZMD L1) |
| Light source flash duration | 2 µs |
| Pixel size | 0.68 µm |
| FL1 description | 525/50 |
|---|---|
| Laser 1 output power | 35 % |
| Laser 1 wavelength | 488 nm |
| Lower voltage detection limit | -1 V |
| Number of active channels | 1 |
| Number of active lasers | 1 |
| Number of available channels | 3 |
| Number of available lasers | 3 |
| Rolling median filter size for traces | 7 |
| Samples per event | 680 |
| Trace bit depth | 16 |
| Trace sample rate | 1e+06 Hz |
| Upper voltage detection limit | 1 V |
Additional Information
| Field | Value |
|---|---|
| license | Creative Commons Public Domain Dedication |
| created | 4 years ago |
| format | RT-FDC |
| id | 57d796ef-6936-7a3f-ddcf-6b82903f4722 |
| metadata modified | 1 year ago |
| mimetype | RT-DC |
| package id | e61bb335-3523-e539-78d1-0720ad5a99f1 |
| position | 1 |
| s3 available | True |
| s3 url | https://objectstore.hpccloud.mpcdf.mpg.de/circle-5a7a053d-55fb-4f99-960c-f478d0bd418f/resource/57d/796/ef-6936-7a3f-ddcf-6b82903f4722 |
| sha256 | 066cd56b79db4078039d3ad2af60c008a32c63c491fdc2af107acc175c4135c6 |
| size | 332.1 MiB |
| dc:experiment:date | 2019-05-11 |
| dc:experiment:event count | 50,453 |
| dc:experiment:run index | 1 |
| dc:experiment:sample | Figure1d_01_Beads_FL_Initial |
| dc:experiment:time | 19:29:59 |
| dc:fluorescence:bit depth | 16 |
| dc:fluorescence:channel 1 name | 525/50 |
| dc:fluorescence:channel count | 1 |
| dc:fluorescence:channels installed | 3 |
| dc:fluorescence:laser 1 lambda | 488 |
| dc:fluorescence:laser 1 power | 35 |
| dc:fluorescence:laser count | 1 |
| dc:fluorescence:lasers installed | 3 |
| dc:fluorescence:sample rate | 1,000,000 |
| dc:fluorescence:samples per event | 680 |
| dc:fluorescence:signal max | 1 |
| dc:fluorescence:signal min | -1 |
| dc:fluorescence:trace median | 7 |
| dc:imaging:flash device | LED (ZMD L1) |
| dc:imaging:flash duration | 2 |
| dc:imaging:frame rate | 2,500 |
| dc:imaging:pixel size | 0.68 |
| dc:imaging:roi position x | 629 |
| dc:imaging:roi position y | 512 |
| dc:imaging:roi size x | 100 |
| dc:imaging:roi size y | 40 |
| dc:online contour:bin area min | 10 |
| dc:online contour:bin kernel | 5 |
| dc:online contour:bin threshold | -6 |
| dc:online contour:image blur | 0 |
| dc:online contour:no absdiff | True |
| dc:online filter:area ratio max | 1.2 |
| dc:online filter:area ratio min | 1 |
| dc:online filter:area ratio soft limit | True |
| dc:online filter:area um max | 150 |
| dc:online filter:area um min | 70 |
| dc:online filter:area um soft limit | True |
| dc:online filter:fl1 max max | 10,000 |
| dc:online filter:fl1 max min | 500 |
| dc:online filter:fl1 max soft limit | True |
| dc:online filter:size x max | 80 |
| dc:online filter:size x min | 1 |
| dc:online filter:size x soft limit | False |
| dc:online filter:size y max | 80 |
| dc:online filter:size y min | 1 |
| dc:online filter:size y soft limit | False |
| dc:setup:channel width | 20 |
| dc:setup:chip region | channel |
| dc:setup:flow rate | 0.04 |
| dc:setup:flow rate sample | 0.01 |
| dc:setup:flow rate sheath | 0.03 |
| dc:setup:identifier | ZMDD-AcC-fe3cdd-2a2250 |
| dc:setup:medium | CellCarrierB |
| dc:setup:module composition | AcCellerator,FluorescenceModule |
| dc:setup:software version | ShapeIn 2.0.7 |