20230313_FCR3_panned_unstained.rtdc
| Date of measurement | 2023-03-13 |
|---|---|
| Index of measurement run | 1 |
| Sample name | 20230313_FCR3_panned_unstained |
| Number of recorded events | 1.543e+05 |
| Start time of measurement | 11:37:13 |
| Software | 2.3.4.54 | dclab 0.48.1 |
|---|---|
| Modules used | AcCellerator, FluorescenceModule |
| Flow rate in channel | 0.02 µL/s |
| Imaged chip region | channel |
| Mean chip temperature | 23 °C |
| Medium used | 0.59% MC-PBS |
| Sample flow rate | 0.005 µL/s |
| Sheath flow rate | 0.015 µL/s |
| Unique setup identifier | ZMDD-AcC-774f84-3da488 |
| Width of microfluidic channel | 20 µm |
| Image height | 80 px |
|---|---|
| Image width | 250 px |
| Image x coordinate on sensor | 533 px |
| Image y coordinate on sensor | 470 px |
| Imaging frame rate | 625 Hz |
| Light source device type | LED (ZMD L1) |
| Light source flash duration | 2 µs |
| Pixel size | 0.339 µm |
| Baseline offset channel 1 | -25 |
|---|---|
| Baseline offset channel 2 | -5 |
| Baseline offset channel 3 | -1 |
| FL1 description | 525/50 |
| FL2 description | 593/46 |
| FL3 description | 700/75 |
| Laser 1 output power | 100 % |
| Laser 1 wavelength | 488 nm |
| Laser 3 output power | 100 % |
| Laser 3 wavelength | 640 nm |
| Lower voltage detection limit | -1 V |
| Number of active channels | 3 |
| Number of active lasers | 2 |
| Number of available channels | 3 |
| Number of available lasers | 3 |
| Rolling median filter size for traces | 5 |
| Samples per event | 1059 |
| Trace bit depth | 16 |
| Trace sample rate | 6.25e+05 Hz |
| Upper voltage detection limit | 1 V |
Additional Information
| Field | Value |
|---|---|
| license | Creative Commons Public Domain Dedication |
| created | 2 years ago |
| format | RT-FDC |
| id | 130adc8a-1619-4abd-b450-4da1b6b0007b |
| metadata modified | 1 year ago |
| mimetype | RT-DC |
| package id | 9c71a85e-edff-45f7-bd89-ec51b7a4efea |
| position | 2 |
| s3 available | True |
| s3 url | https://objectstore.hpccloud.mpcdf.mpg.de/circle-ff780b54-103c-4827-a19a-8efce15ba307/resource/130/adc/8a-1619-4abd-b450-4da1b6b0007b |
| sha256 | e103c30caae01c2a1b1f4243919ad8bc74a11be5003ef433fce630ab2657324d |
| size | 1.7 GiB |
| dc:experiment:date | 2023-03-13 |
| dc:experiment:event count | 154,279 |
| dc:experiment:run index | 1 |
| dc:experiment:sample | 20230313_FCR3_panned_unstained |
| dc:experiment:time | 11:37:13 |
| dc:fluorescence:baseline 1 offset | -25 |
| dc:fluorescence:baseline 2 offset | -5 |
| dc:fluorescence:baseline 3 offset | -1 |
| dc:fluorescence:bit depth | 16 |
| dc:fluorescence:channel 1 name | 525/50 |
| dc:fluorescence:channel 2 name | 593/46 |
| dc:fluorescence:channel 3 name | 700/75 |
| dc:fluorescence:channel count | 3 |
| dc:fluorescence:channels installed | 3 |
| dc:fluorescence:laser 1 lambda | 488 |
| dc:fluorescence:laser 1 power | 100 |
| dc:fluorescence:laser 3 lambda | 640 |
| dc:fluorescence:laser 3 power | 100 |
| dc:fluorescence:laser count | 2 |
| dc:fluorescence:lasers installed | 3 |
| dc:fluorescence:sample rate | 625,000 |
| dc:fluorescence:samples per event | 1,059 |
| dc:fluorescence:signal max | 1 |
| dc:fluorescence:signal min | -1 |
| dc:fluorescence:trace median | 5 |
| dc:imaging:flash device | LED (ZMD L1) |
| dc:imaging:flash duration | 2 |
| dc:imaging:frame rate | 625 |
| dc:imaging:pixel size | 0.339 |
| dc:imaging:roi position x | 533 |
| dc:imaging:roi position y | 470 |
| dc:imaging:roi size x | 250 |
| dc:imaging:roi size y | 80 |
| dc:online contour:bg empty | True |
| dc:online contour:bin area min | 10 |
| dc:online contour:bin kernel | 5 |
| dc:online contour:bin threshold | -6 |
| dc:online contour:image blur | 0 |
| dc:online contour:no absdiff | True |
| dc:setup:channel width | 20 |
| dc:setup:chip region | channel |
| dc:setup:flow rate | 0.02 |
| dc:setup:flow rate sample | 0.005 |
| dc:setup:flow rate sheath | 0.015 |
| dc:setup:identifier | ZMDD-AcC-774f84-3da488 |
| dc:setup:medium | CellCarrierB |
| dc:setup:module composition | AcCellerator,FluorescenceModule |
| dc:setup:software version | 2.3.4.54 | dclab 0.48.1 |
| dc:setup:temperature | 23 |