M2_data.rtdc
0.32ul/s
| Date of measurement | 2019-03-16 |
|---|---|
| Index of measurement run | 2 |
| Sample name | Unstained |
| Number of recorded events | 1.001e+04 |
| Start time of measurement | 13:03:42 |
| Software | ShapeIn 2.0.5 |
|---|---|
| Modules used | AcCellerator, FluorescenceModule |
| Flow rate in channel | 0.32 µL/s |
| Imaged chip region | channel |
| Medium used | 0.49% MC-PBS |
| Sample flow rate | 0.08 µL/s |
| Sheath flow rate | 0.24 µL/s |
| Unique setup identifier | ZMDD-AcC-524148-45fde0 |
| Width of microfluidic channel | 30 µm |
| Image height | 100 px |
|---|---|
| Image width | 250 px |
| Image x coordinate on sensor | 701 px |
| Image y coordinate on sensor | 512 px |
| Imaging frame rate | 4000 Hz |
| Light source device type | LED (ZMD L1) |
| Light source flash duration | 2 µs |
| Pixel size | 0.34 µm |
| FL1 description | 525/50 (e.g. FITC, GFP) |
|---|---|
| FL2 description | 593/46 (e.g. PE, mCherry) |
| Laser 2 output power | 20 % |
| Laser 2 wavelength | 561 nm |
| Lower voltage detection limit | -1 V |
| Number of active channels | 2 |
| Number of active lasers | 1 |
| Number of available channels | 3 |
| Number of available lasers | 3 |
| Rolling median filter size for traces | 7 |
| Samples per event | 239 |
| Trace bit depth | 16 |
| Trace sample rate | 1e+06 Hz |
| Upper voltage detection limit | 1 V |
Additional Information
| Field | Value |
|---|---|
| license | Creative Commons Attribution-NonCommercial 4.0 |
| created | 4 years ago |
| format | RT-FDC |
| id | b766b1d8-6750-48d4-8f4a-a9293c3c3826 |
| metadata modified | 2 years ago |
| mimetype | RT-DC |
| package id | 4ef461c9-61bc-4e24-a30c-5345fbde159a |
| position | 1 |
| s3 available | True |
| s3 url | https://objectstore.hpccloud.mpcdf.mpg.de/circle-b255d141-a13c-42c5-9558-9951ac8cf233/resource/b76/6b1/d8-6750-48d4-8f4a-a9293c3c3826 |
| sha256 | 7681fa11740093d4dbbee850adb10d66bcfce53e91d2864cccef6592c8587758 |
| size | 261.6 MiB |
| dc:experiment:date | 2019-03-16 |
| dc:experiment:event count | 10,009 |
| dc:experiment:run index | 2 |
| dc:experiment:sample | Unstained |
| dc:experiment:time | 13:03:42 |
| dc:fluorescence:bit depth | 16 |
| dc:fluorescence:channel 1 name | 525/50 (e.g. FITC, GFP) |
| dc:fluorescence:channel 2 name | 593/46 (e.g. PE, mCherry) |
| dc:fluorescence:channel count | 2 |
| dc:fluorescence:channels installed | 3 |
| dc:fluorescence:laser 2 lambda | 561 |
| dc:fluorescence:laser 2 power | 20 |
| dc:fluorescence:laser count | 1 |
| dc:fluorescence:lasers installed | 3 |
| dc:fluorescence:sample rate | 1,000,000 |
| dc:fluorescence:samples per event | 239 |
| dc:fluorescence:signal max | 1 |
| dc:fluorescence:signal min | -1 |
| dc:fluorescence:trace median | 7 |
| dc:imaging:flash device | LED (ZMD L1) |
| dc:imaging:flash duration | 2 |
| dc:imaging:frame rate | 4,000 |
| dc:imaging:pixel size | 0.34 |
| dc:imaging:roi position x | 701 |
| dc:imaging:roi position y | 512 |
| dc:imaging:roi size x | 250 |
| dc:imaging:roi size y | 100 |
| dc:online contour:bin area min | 10 |
| dc:online contour:bin kernel | 5 |
| dc:online contour:bin threshold | -6 |
| dc:online contour:image blur | 0 |
| dc:online contour:no absdiff | True |
| dc:online filter:size x max | 80 |
| dc:online filter:size x min | 1 |
| dc:online filter:size x soft limit | False |
| dc:online filter:size y max | 80 |
| dc:online filter:size y min | 1 |
| dc:online filter:size y soft limit | False |
| dc:online filter:target event count | 10,000 |
| dc:setup:channel width | 30 |
| dc:setup:chip region | channel |
| dc:setup:flow rate | 0.32 |
| dc:setup:flow rate sample | 0.08 |
| dc:setup:flow rate sheath | 0.24 |
| dc:setup:identifier | ZMDD-AcC-524148-45fde0 |
| dc:setup:medium | CellCarrier |
| dc:setup:module composition | AcCellerator,FluorescenceModule |
| dc:setup:software version | ShapeIn 2.0.5 |