20170521_1651_phos1_A2.rtdc
| Date of measurement | 2017-05-21 |
|---|---|
| Index of measurement run | 1 |
| Sample name | 20170521_1651_dsRNA_A2_col |
| Number of recorded events | 8035 |
| Start time of measurement | 16:51:10 |
| Unique measurement identifier | None-afc3 |
| Software | fRT-DC V1.06170206 | dclab 0.60.3 |
|---|---|
| Modules used | Cell_Flow_2, Fluor |
| Flow rate in channel | 0.04 µL/s |
| Imaged chip region | channel |
| Width of microfluidic channel | 20 µm |
| Image height | 96 px |
|---|---|
| Image width | 256 px |
| Image x coordinate on sensor | 504 px |
| Image y coordinate on sensor | 472 px |
| Imaging frame rate | 2000 Hz |
| Light source device type | LED (undefined) |
| Light source flash duration | 2 µs |
| Pixel size | 0.34 µm |
| FL1 description | FL1 |
|---|---|
| FL2 description | FL2 |
| Laser 1 output power | 10 % |
| Laser 1 wavelength | 488 nm |
| Laser 2 output power | 35 % |
| Laser 2 wavelength | 561 nm |
| Laser 3 output power | 0 % |
| Laser 3 wavelength | 640 nm |
| Lower voltage detection limit | -1 V |
| Number of active channels | 2 |
| Number of active lasers | 2 |
| Number of available channels | 3 |
| Number of available lasers | 3 |
| Rolling median filter size for traces | 5 |
| Samples per event | 300 |
| Trace bit depth | 16 |
| Trace sample rate | 5e+05 Hz |
| Upper voltage detection limit | 1 V |
Additional Information
| Field | Value |
|---|---|
| license | Creative Commons Public Domain Dedication |
| created | 2 months ago |
| etag | 9573daec2baffb14943f895aecccfe43 |
| format | RT-FDC |
| id | 09034d1e-7077-493f-b661-e7fbee208f4e |
| metadata modified | 2 months ago |
| mimetype | RT-DC |
| package id | 9383e05b-c6bc-4bbf-a7ef-8de6b80ebdb2 |
| position | 1 |
| s3 available | True |
| s3 url | https://objectstore.hpccloud.mpcdf.mpg.de/circle-5d2846fc-f12d-45de-96d1-e1eea06e16c7/resource/090/34d/1e-7077-493f-b661-e7fbee208f4e |
| sha256 | 2cd8135bca15821b6b3bf0d75274f1977723e709b7eb5c5a4a25cf90a98ec204 |
| size | 140.5 MiB |
| dc:experiment:date | 2017-05-21 |
| dc:experiment:event count | 8,035 |
| dc:experiment:run identifier | None-afc3 |
| dc:experiment:run index | 1 |
| dc:experiment:sample | 20170521_1651_dsRNA_A2_col |
| dc:experiment:time | 16:51:10 |
| dc:fluorescence:bit depth | 16 |
| dc:fluorescence:channel 1 name | FL1 |
| dc:fluorescence:channel 2 name | FL2 |
| dc:fluorescence:channel count | 2 |
| dc:fluorescence:channels installed | 3 |
| dc:fluorescence:laser 1 lambda | 488 |
| dc:fluorescence:laser 1 power | 10 |
| dc:fluorescence:laser 2 lambda | 561 |
| dc:fluorescence:laser 2 power | 35 |
| dc:fluorescence:laser 3 lambda | 640 |
| dc:fluorescence:laser 3 power | 0 |
| dc:fluorescence:laser count | 2 |
| dc:fluorescence:lasers installed | 3 |
| dc:fluorescence:sample rate | 500,000 |
| dc:fluorescence:samples per event | 300 |
| dc:fluorescence:signal max | 1 |
| dc:fluorescence:signal min | -1 |
| dc:fluorescence:trace median | 5 |
| dc:imaging:flash device | LED (undefined) |
| dc:imaging:flash duration | 2 |
| dc:imaging:frame rate | 2,000 |
| dc:imaging:pixel size | 0.34 |
| dc:imaging:roi position x | 504 |
| dc:imaging:roi position y | 472 |
| dc:imaging:roi size x | 256 |
| dc:imaging:roi size y | 96 |
| dc:online contour:bin area min | 50 |
| dc:online contour:bin kernel | 5 |
| dc:online contour:bin threshold | 6 |
| dc:online contour:image blur | 0 |
| dc:online contour:no absdiff | True |
| dc:setup:channel width | 20 |
| dc:setup:chip region | channel |
| dc:setup:flow rate | 0.04 |
| dc:setup:module composition | Cell_Flow_2, Fluor |
| dc:setup:software version | fRT-DC V1.06170206 | dclab 0.60.3 |
| sp:cells:cell type | KC167 |
| sp:cells:live | True |
| sp:cells:organism | Drosophila melanogaster |
| sp:chip:channel width | 20 |
| sp:experiment:buffer osmolality | 325 |
| sp:experiment:buffer ph | 6.8 |
| sp:experiment:concentration | 5 |
| sp:identifiers:labels | GFP tdTomato |
| sp:identifiers:tags | NLS-GFP |