Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_19.05.09 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "0442007294214f0050c6b81649d85180",
"name": "M1_20170521_1753_dsRNA_A5_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:05:32"
}
}
dclab-tdms2rtdc-warnings MultipleSamplesPerEventFound
in dclab.rtdc_dataset.fmt_tdms.exc line 101:
Ignoring trace data of
'\\syn03.frontend.storage.int.mpl.mpg.de\guck_division2\Members\Laura\20240219_Kasia_screen_all_folders\20170521_katarzyna_kc167_phospho_screen\20170521_1753_dsRNA_A5_col\M1_20170521_1753_dsRNA_A5_col_traces.tdms'
due to multiple values for samples per event: [300 600]
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.2005
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 5.52624e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 0 mean_circ: 0.927146
Ran for 550 frames Cell Events: 5 mean_circ: 0.954666
Ran for 1050 frames Cell Events: 14 mean_circ: 0.950195
Ran for 1550 frames Cell Events: 27 mean_circ: 0.949852
Ran for 2050 frames Cell Events: 41 mean_circ: 0.944504
Ran for 2550 frames Cell Events: 52 mean_circ: 0.943704
Ran for 3050 frames Cell Events: 63 mean_circ: 0.946336
Ran for 3550 frames Cell Events: 76 mean_circ: 0.947663
Ran for 4050 frames Cell Events: 90 mean_circ: 0.943758
Ran for 4550 frames Cell Events: 94 mean_circ: 0.943563
Ran for 5050 frames Cell Events: 111 mean_circ: 0.944255
Ran for 6050 frames Cell Events: 126 mean_circ: 0.946035
Ran for 6550 frames Cell Events: 140 mean_circ: 0.945573
Ran for 7050 frames Cell Events: 155 mean_circ: 0.944519
Ran for 7550 frames Cell Events: 168 mean_circ: 0.943506
Ran for 8050 frames Cell Events: 185 mean_circ: 0.944113
Ran for 9050 frames Cell Events: 201 mean_circ: 0.943889
Ran for 9550 frames Cell Events: 219 mean_circ: 0.944021
Ran for 10050 frames Cell Events: 231 mean_circ: 0.944226
Ran for 10550 frames Cell Events: 238 mean_circ: 0.94379
Ran for 11550 frames Cell Events: 263 mean_circ: 0.942505
Ran for 12550 frames Cell Events: 277 mean_circ: 0.941742
Ran for 13050 frames Cell Events: 282 mean_circ: 0.941891
Ran for 13550 frames Cell Events: 292 mean_circ: 0.942116
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Ran for 15050 frames Cell Events: 314 mean_circ: 0.943626
Ran for 15550 frames Cell Events: 323 mean_circ: 0.943706
Ran for 16050 frames Cell Events: 341 mean_circ: 0.943155
Ran for 16550 frames Cell Events: 354 mean_circ: 0.943796
Ran for 17050 frames Cell Events: 371 mean_circ: 0.943496
Ran for 18050 frames Cell Events: 391 mean_circ: 0.943969
Ran for 18550 frames Cell Events: 417 mean_circ: 0.942582
Ran for 19050 frames Cell Events: 437 mean_circ: 0.942167
Ran for 19550 frames Cell Events: 445 mean_circ: 0.941835
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Ran for 23050 frames Cell Events: 533 mean_circ: 0.942806
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Ran for 24050 frames Cell Events: 550 mean_circ: 0.9429
Ran for 25550 frames Cell Events: 591 mean_circ: 0.942458
Ran for 26050 frames Cell Events: 598 mean_circ: 0.942043
Ran for 27050 frames Cell Events: 611 mean_circ: 0.942516
Ran for 28050 frames Cell Events: 629 mean_circ: 0.942652
Ran for 29050 frames Cell Events: 647 mean_circ: 0.942706
Ran for 29550 frames Cell Events: 655 mean_circ: 0.94292
Ran for 30050 frames Cell Events: 670 mean_circ: 0.942509
Ran for 30550 frames Cell Events: 689 mean_circ: 0.942671
Ran for 31050 frames Cell Events: 703 mean_circ: 0.942014
Ran for 31550 frames Cell Events: 711 mean_circ: 0.941863
Ran for 32050 frames Cell Events: 731 mean_circ: 0.941872
Ran for 32550 frames Cell Events: 745 mean_circ: 0.941913
Ran for 33050 frames Cell Events: 749 mean_circ: 0.941986
Ran for 33550 frames Cell Events: 759 mean_circ: 0.94199
Ran for 34050 frames Cell Events: 769 mean_circ: 0.942008
Ran for 34550 frames Cell Events: 784 mean_circ: 0.942172
Ran for 36050 frames Cell Events: 820 mean_circ: 0.941928
Ran for 36550 frames Cell Events: 828 mean_circ: 0.94178
Ran for 37050 frames Cell Events: 846 mean_circ: 0.941886
Ran for 38050 frames Cell Events: 876 mean_circ: 0.942033
Ran for 39050 frames Cell Events: 901 mean_circ: 0.94203
Ran for 39550 frames Cell Events: 905 mean_circ: 0.942142
Ran for 40550 frames Cell Events: 933 mean_circ: 0.941728
Ran for 42050 frames Cell Events: 981 mean_circ: 0.94166
Ran for 42550 frames Cell Events: 988 mean_circ: 0.941196
Ran for 43050 frames Cell Events: 1004 mean_circ: 0.940967
Ran for 43550 frames Cell Events: 1016 mean_circ: 0.940815
Ran for 44050 frames Cell Events: 1030 mean_circ: 0.940477
Ran for 45050 frames Cell Events: 1052 mean_circ: 0.940663
Ran for 45550 frames Cell Events: 1064 mean_circ: 0.940721
Ran for 46050 frames Cell Events: 1073 mean_circ: 0.940832
Ran for 46550 frames Cell Events: 1084 mean_circ: 0.940773
Ran for 47050 frames Cell Events: 1102 mean_circ: 0.941071
Ran for 47550 frames Cell Events: 1112 mean_circ: 0.941164
Ran for 48050 frames Cell Events: 1122 mean_circ: 0.941136
Ran for 48550 frames Cell Events: 1131 mean_circ: 0.941264
Ran for 49050 frames Cell Events: 1145 mean_circ: 0.941154
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Ran for 50550 frames Cell Events: 1195 mean_circ: 0.941186
Ran for 51550 frames Cell Events: 1213 mean_circ: 0.941026
Ran for 52050 frames Cell Events: 1222 mean_circ: 0.940814
Ran for 53050 frames Cell Events: 1242 mean_circ: 0.940933
Ran for 53550 frames Cell Events: 1251 mean_circ: 0.941046
Ran for 54050 frames Cell Events: 1261 mean_circ: 0.941078
Ran for 55550 frames Cell Events: 1280 mean_circ: 0.940848
Ran for 56050 frames Cell Events: 1289 mean_circ: 0.940834
Ran for 56550 frames Cell Events: 1296 mean_circ: 0.940722
Ran for 57050 frames Cell Events: 1305 mean_circ: 0.940675
Ran for 58050 frames Cell Events: 1329 mean_circ: 0.940695
Ran for 58550 frames Cell Events: 1344 mean_circ: 0.94058
Ran for 59050 frames Cell Events: 1355 mean_circ: 0.940625
Ran for 59550 frames Cell Events: 1371 mean_circ: 0.940716
Ran for 60050 frames Cell Events: 1379 mean_circ: 0.940768
Ran for 60550 frames Cell Events: 1390 mean_circ: 0.940792
Ran for 61050 frames Cell Events: 1398 mean_circ: 0.940694
Ran for 61550 frames Cell Events: 1409 mean_circ: 0.940737
Ran for 62050 frames Cell Events: 1419 mean_circ: 0.940736
Ran for 62550 frames Cell Events: 1440 mean_circ: 0.940993
Ran for 63050 frames Cell Events: 1460 mean_circ: 0.940759
Ran for 63550 frames Cell Events: 1467 mean_circ: 0.940739
Ran for 64550 frames Cell Events: 1495 mean_circ: 0.940456
Ran for 65550 frames Cell Events: 1514 mean_circ: 0.940518
Ran for 66050 frames Cell Events: 1539 mean_circ: 0.940759
Ran for 67050 frames Cell Events: 1562 mean_circ: 0.940932
Ran for 67550 frames Cell Events: 1582 mean_circ: 0.940996
Ran for 68050 frames Cell Events: 1590 mean_circ: 0.941051
Ran for 69050 frames Cell Events: 1606 mean_circ: 0.941161
Ran for 70050 frames Cell Events: 1625 mean_circ: 0.941113
Ran for 70550 frames Cell Events: 1635 mean_circ: 0.941105
Ran for 71550 frames Cell Events: 1654 mean_circ: 0.941136
Ran for 72050 frames Cell Events: 1671 mean_circ: 0.941104
Ran for 72550 frames Cell Events: 1679 mean_circ: 0.941101
Ran for 73550 frames Cell Events: 1703 mean_circ: 0.941122
Ran for 74550 frames Cell Events: 1725 mean_circ: 0.941269
Ran for 75050 frames Cell Events: 1742 mean_circ: 0.94133
Ran for 76050 frames Cell Events: 1767 mean_circ: 0.941292
Ran for 76550 frames Cell Events: 1779 mean_circ: 0.94141
Ran for 77050 frames Cell Events: 1790 mean_circ: 0.941405
Ran for 77550 frames Cell Events: 1810 mean_circ: 0.941505
Ran for 78550 frames Cell Events: 1837 mean_circ: 0.941506
Ran for 79050 frames Cell Events: 1858 mean_circ: 0.941507
Ran for 79550 frames Cell Events: 1869 mean_circ: 0.941388
Ran for 80050 frames Cell Events: 1876 mean_circ: 0.941301
Ran for 80550 frames Cell Events: 1887 mean_circ: 0.941125
Ran for 81050 frames Cell Events: 1895 mean_circ: 0.94105
Ran for 81550 frames Cell Events: 1906 mean_circ: 0.941114
Ran for 82050 frames Cell Events: 1921 mean_circ: 0.941223
Ran for 82550 frames Cell Events: 1933 mean_circ: 0.941232
Ran for 83050 frames Cell Events: 1942 mean_circ: 0.9412
Ran for 84550 frames Cell Events: 1969 mean_circ: 0.941387
Ran for 85050 frames Cell Events: 1972 mean_circ: 0.941382
Ran for 85550 frames Cell Events: 1980 mean_circ: 0.94139
Ran for 86050 frames Cell Events: 1991 mean_circ: 0.941295
Ran for 86550 frames Cell Events: 2001 mean_circ: 0.941339
Ran for 87050 frames Cell Events: 2010 mean_circ: 0.94129
Ran for 88050 frames Cell Events: 2032 mean_circ: 0.941257
Ran for 88550 frames Cell Events: 2039 mean_circ: 0.941292
Ran for 89050 frames Cell Events: 2049 mean_circ: 0.941306
Ran for 89550 frames Cell Events: 2065 mean_circ: 0.941219
Ran for 90050 frames Cell Events: 2080 mean_circ: 0.941057
Ran for 90550 frames Cell Events: 2089 mean_circ: 0.941078
Ran for 91050 frames Cell Events: 2095 mean_circ: 0.94115
Ran for 91550 frames Cell Events: 2109 mean_circ: 0.941102
Ran for 92550 frames Cell Events: 2130 mean_circ: 0.941174
Ran for 93050 frames Cell Events: 2140 mean_circ: 0.941201
Ran for 94050 frames Cell Events: 2167 mean_circ: 0.941344
Ran for 94550 frames Cell Events: 2176 mean_circ: 0.941319
Ran for 95050 frames Cell Events: 2189 mean_circ: 0.941373
Ran for 95550 frames Cell Events: 2200 mean_circ: 0.941274
Ran for 96550 frames Cell Events: 2219 mean_circ: 0.941199
Ran for 97050 frames Cell Events: 2226 mean_circ: 0.94118
Ran for 97550 frames Cell Events: 2234 mean_circ: 0.94122
Ran for 98050 frames Cell Events: 2247 mean_circ: 0.941201
Ran for 98550 frames Cell Events: 2258 mean_circ: 0.94116
Ran for 99050 frames Cell Events: 2267 mean_circ: 0.941159
Ran for 99550 frames Cell Events: 2282 mean_circ: 0.941044
Ran for 100050 frames Cell Events: 2304 mean_circ: 0.940979
Ran for 100550 frames Cell Events: 2315 mean_circ: 0.941065
Ran for 101050 frames Cell Events: 2329 mean_circ: 0.940955
Ran for 102050 frames Cell Events: 2363 mean_circ: 0.940873
Ran for 103550 frames Cell Events: 2413 mean_circ: 0.940922
Ran for 104050 frames Cell Events: 2423 mean_circ: 0.940962
Ran for 105050 frames Cell Events: 2457 mean_circ: 0.940939
Ran for 106050 frames Cell Events: 2487 mean_circ: 0.940919
Ran for 107050 frames Cell Events: 2504 mean_circ: 0.941001
Ran for 107550 frames Cell Events: 2511 mean_circ: 0.941
Ran for 108050 frames Cell Events: 2520 mean_circ: 0.940979
Ran for 108550 frames Cell Events: 2533 mean_circ: 0.94098
Ran for 109050 frames Cell Events: 2541 mean_circ: 0.941005
Ran for 109550 frames Cell Events: 2550 mean_circ: 0.940992
Ran for 110050 frames Cell Events: 2563 mean_circ: 0.94104
Ran for 110550 frames Cell Events: 2576 mean_circ: 0.941042
Ran for 111050 frames Cell Events: 2590 mean_circ: 0.941121
Ran for 111550 frames Cell Events: 2610 mean_circ: 0.941102
Ran for 112050 frames Cell Events: 2629 mean_circ: 0.941181
Ran for 112550 frames Cell Events: 2636 mean_circ: 0.941131
Ran for 113550 frames Cell Events: 2660 mean_circ: 0.941079
Ran for 114050 frames Cell Events: 2668 mean_circ: 0.941094
Ran for 114550 frames Cell Events: 2683 mean_circ: 0.941136
Ran for 115050 frames Cell Events: 2694 mean_circ: 0.941194
Ran for 116050 frames Cell Events: 2719 mean_circ: 0.941213
Ran for 117050 frames Cell Events: 2738 mean_circ: 0.941234
Ran for 117550 frames Cell Events: 2750 mean_circ: 0.941181
Ran for 118050 frames Cell Events: 2761 mean_circ: 0.941148
Ran for 118550 frames Cell Events: 2773 mean_circ: 0.941145
Ran for 119550 frames Cell Events: 2783 mean_circ: 0.941193
Ran for 120050 frames Cell Events: 2802 mean_circ: 0.941253
Ran for 120550 frames Cell Events: 2807 mean_circ: 0.941278
Ran for 121050 frames Cell Events: 2816 mean_circ: 0.941324
Ran for 121550 frames Cell Events: 2822 mean_circ: 0.94134
Ran for 123050 frames Cell Events: 2848 mean_circ: 0.941424
Ran for 124050 frames Cell Events: 2856 mean_circ: 0.941402
Ran for 124550 frames Cell Events: 2879 mean_circ: 0.941379
Ran for 125050 frames Cell Events: 2889 mean_circ: 0.941436
Ran for 125550 frames Cell Events: 2904 mean_circ: 0.941447
Ran for 126050 frames Cell Events: 2915 mean_circ: 0.941517
Ran for 126550 frames Cell Events: 2929 mean_circ: 0.941525
Ran for 127550 frames Cell Events: 2950 mean_circ: 0.941631
Ran for 129050 frames Cell Events: 2976 mean_circ: 0.941692
Ran for 129550 frames Cell Events: 2984 mean_circ: 0.941694
Ran for 130050 frames Cell Events: 2994 mean_circ: 0.941691
Ran for 130550 frames Cell Events: 3008 mean_circ: 0.941731
Ran for 131050 frames Cell Events: 3027 mean_circ: 0.941626
Ran for 131550 frames Cell Events: 3038 mean_circ: 0.941736
Ran for 132050 frames Cell Events: 3048 mean_circ: 0.941713
Ran for 133050 frames Cell Events: 3072 mean_circ: 0.941778
Ran for 133550 frames Cell Events: 3084 mean_circ: 0.941845
Ran for 134050 frames Cell Events: 3097 mean_circ: 0.941838
Ran for 134550 frames Cell Events: 3107 mean_circ: 0.941864
Ran for 135050 frames Cell Events: 3117 mean_circ: 0.941882
Ran for 135550 frames Cell Events: 3128 mean_circ: 0.941895
Ran for 136050 frames Cell Events: 3140 mean_circ: 0.941955
Ran for 136550 frames Cell Events: 3157 mean_circ: 0.941909
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Ran for 138050 frames Cell Events: 3180 mean_circ: 0.942008
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Ran for 141550 frames Cell Events: 3303 mean_circ: 0.941733
Ran for 142050 frames Cell Events: 3322 mean_circ: 0.941648
Ran for 143050 frames Cell Events: 3349 mean_circ: 0.941731
Ran for 144550 frames Cell Events: 3396 mean_circ: 0.941706
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Ran for 146550 frames Cell Events: 3443 mean_circ: 0.94178
Ran for 147550 frames Cell Events: 3469 mean_circ: 0.941844
Ran for 148050 frames Cell Events: 3484 mean_circ: 0.941829
Ran for 148550 frames Cell Events: 3495 mean_circ: 0.941856
Ran for 149050 frames Cell Events: 3509 mean_circ: 0.941875
Ran for 150050 frames Cell Events: 3531 mean_circ: 0.941821
Ran for 150550 frames Cell Events: 3542 mean_circ: 0.94183
Ran for 151050 frames Cell Events: 3557 mean_circ: 0.941841
Ran for 151550 frames Cell Events: 3575 mean_circ: 0.941863
Ran for 152050 frames Cell Events: 3587 mean_circ: 0.941869
Ran for 152550 frames Cell Events: 3596 mean_circ: 0.941892
Ran for 153050 frames Cell Events: 3608 mean_circ: 0.941902
Ran for 153550 frames Cell Events: 3615 mean_circ: 0.941918
Ran for 154550 frames Cell Events: 3642 mean_circ: 0.941949
Ran for 155550 frames Cell Events: 3664 mean_circ: 0.942012
Ran for 156050 frames Cell Events: 3682 mean_circ: 0.941999
Ran for 156550 frames Cell Events: 3691 mean_circ: 0.942006
Ran for 157050 frames Cell Events: 3701 mean_circ: 0.94202
Ran for 157550 frames Cell Events: 3712 mean_circ: 0.942061
Ran for 158050 frames Cell Events: 3721 mean_circ: 0.942073
Ran for 159050 frames Cell Events: 3743 mean_circ: 0.942115
Ran for 159550 frames Cell Events: 3747 mean_circ: 0.942137
Ran for 160050 frames Cell Events: 3756 mean_circ: 0.942091
Ran for 161050 frames Cell Events: 3772 mean_circ: 0.942071
Ran for 161550 frames Cell Events: 3784 mean_circ: 0.942067
Ran for 162050 frames Cell Events: 3801 mean_circ: 0.941949
Ran for 162550 frames Cell Events: 3817 mean_circ: 0.941931
Ran for 164050 frames Cell Events: 3863 mean_circ: 0.942011
Ran for 164550 frames Cell Events: 3878 mean_circ: 0.942008
Ran for 165050 frames Cell Events: 3884 mean_circ: 0.94195
Ran for 165550 frames Cell Events: 3896 mean_circ: 0.941922
Ran for 166050 frames Cell Events: 3905 mean_circ: 0.941859
Ran for 166550 frames Cell Events: 3935 mean_circ: 0.941883
Ran for 167050 frames Cell Events: 3953 mean_circ: 0.941838
Ran for 167550 frames Cell Events: 3962 mean_circ: 0.941792
Ran for 168550 frames Cell Events: 3982 mean_circ: 0.941793
Ran for 169050 frames Cell Events: 3998 mean_circ: 0.941789
Ran for 169550 frames Cell Events: 4007 mean_circ: 0.941823
Ran for 170050 frames Cell Events: 4018 mean_circ: 0.941832
Ran for 170550 frames Cell Events: 4031 mean_circ: 0.941877
Ran for 172050 frames Cell Events: 4065 mean_circ: 0.941834
Ran for 172550 frames Cell Events: 4076 mean_circ: 0.941846
Ran for 173550 frames Cell Events: 4103 mean_circ: 0.941811
Ran for 174050 frames Cell Events: 4115 mean_circ: 0.941836
Ran for 174550 frames Cell Events: 4123 mean_circ: 0.941779
Ran for 175550 frames Cell Events: 4146 mean_circ: 0.941765
Ran for 176050 frames Cell Events: 4154 mean_circ: 0.941762
Ran for 176550 frames Cell Events: 4162 mean_circ: 0.941789
Ran for 177050 frames Cell Events: 4177 mean_circ: 0.941764
Ran for 177550 frames Cell Events: 4186 mean_circ: 0.941702
Ran for 178050 frames Cell Events: 4199 mean_circ: 0.94167
Ran for 178550 frames Cell Events: 4212 mean_circ: 0.941582
Ran for 179050 frames Cell Events: 4228 mean_circ: 0.941639
Ran for 180050 frames Cell Events: 4258 mean_circ: 0.941738
Ran for 180550 frames Cell Events: 4267 mean_circ: 0.941748
Ran for 181050 frames Cell Events: 4278 mean_circ: 0.941747
Ran for 181550 frames Cell Events: 4295 mean_circ: 0.941771
Ran for 182550 frames Cell Events: 4320 mean_circ: 0.941797
Ran for 183050 frames Cell Events: 4337 mean_circ: 0.941785
Ran for 183550 frames Cell Events: 4357 mean_circ: 0.941719
Ran for 184050 frames Cell Events: 4374 mean_circ: 0.941819
Ran for 184550 frames Cell Events: 4393 mean_circ: 0.941845
Ran for 185050 frames Cell Events: 4402 mean_circ: 0.941839
Ran for 185550 frames Cell Events: 4417 mean_circ: 0.94179
Ran for 186050 frames Cell Events: 4436 mean_circ: 0.941784
Ran for 186550 frames Cell Events: 4450 mean_circ: 0.941812
Ran for 187050 frames Cell Events: 4462 mean_circ: 0.941658
Ran for 187550 frames Cell Events: 4469 mean_circ: 0.941648
Ran for 188050 frames Cell Events: 4482 mean_circ: 0.941688
Ran for 188550 frames Cell Events: 4500 mean_circ: 0.941648
Ran for 189050 frames Cell Events: 4516 mean_circ: 0.941615
Ran for 189550 frames Cell Events: 4528 mean_circ: 0.941545
Ran for 190050 frames Cell Events: 4548 mean_circ: 0.941608
Ran for 190550 frames Cell Events: 4560 mean_circ: 0.941548
Ran for 191050 frames Cell Events: 4573 mean_circ: 0.941583
Ran for 191550 frames Cell Events: 4597 mean_circ: 0.941636
Ran for 192050 frames Cell Events: 4614 mean_circ: 0.941612
Ran for 192550 frames Cell Events: 4624 mean_circ: 0.94164
Ran for 193550 frames Cell Events: 4649 mean_circ: 0.941662
Ran for 194050 frames Cell Events: 4666 mean_circ: 0.94168
Ran for 194550 frames Cell Events: 4683 mean_circ: 0.941678
Ran for 195050 frames Cell Events: 4703 mean_circ: 0.941652
Ran for 195550 frames Cell Events: 4709 mean_circ: 0.941633
Ran for 196050 frames Cell Events: 4724 mean_circ: 0.941635
Ran for 196550 frames Cell Events: 4736 mean_circ: 0.941654
Ran for 197050 frames Cell Events: 4749 mean_circ: 0.94165
Ran for 198050 frames Cell Events: 4779 mean_circ: 0.941728
Ran for 198550 frames Cell Events: 4789 mean_circ: 0.941724
Ran for 199050 frames Cell Events: 4797 mean_circ: 0.941687
Ran for 200050 frames Cell Events: 4817 mean_circ: 0.941684
Ran for 201050 frames Cell Events: 4842 mean_circ: 0.941559
Ran for 201550 frames Cell Events: 4847 mean_circ: 0.941556
Ran for 202050 frames Cell Events: 4858 mean_circ: 0.94157
Ran for 202550 frames Cell Events: 4869 mean_circ: 0.941577
Ran for 203050 frames Cell Events: 4879 mean_circ: 0.941602
Ran for 203550 frames Cell Events: 4887 mean_circ: 0.941621
Ran for 204050 frames Cell Events: 4893 mean_circ: 0.941622
Ran for 204550 frames Cell Events: 4901 mean_circ: 0.94166
Ran for 205050 frames Cell Events: 4924 mean_circ: 0.941561
Ran for 206050 frames Cell Events: 4962 mean_circ: 0.941583
Ran for 206550 frames Cell Events: 4968 mean_circ: 0.94155
Ran for 207050 frames Cell Events: 4978 mean_circ: 0.941516
Ran for 207550 frames Cell Events: 4991 mean_circ: 0.941496
Ran for 208050 frames Cell Events: 5003 mean_circ: 0.941516
Ran for 209550 frames Cell Events: 5054 mean_circ: 0.941492
Ran for 210050 frames Cell Events: 5068 mean_circ: 0.941487
Ran for 210550 frames Cell Events: 5076 mean_circ: 0.941501
Ran for 211550 frames Cell Events: 5098 mean_circ: 0.941486
Ran for 212050 frames Cell Events: 5112 mean_circ: 0.941537
Ran for 213550 frames Cell Events: 5153 mean_circ: 0.941509
Ran for 214050 frames Cell Events: 5159 mean_circ: 0.941499
Ran for 215550 frames Cell Events: 5178 mean_circ: 0.941319
Ran for 216050 frames Cell Events: 5190 mean_circ: 0.941369
Ran for 216550 frames Cell Events: 5202 mean_circ: 0.94142
Ran for 217050 frames Cell Events: 5213 mean_circ: 0.941396
Ran for 217550 frames Cell Events: 5229 mean_circ: 0.941379
Ran for 218050 frames Cell Events: 5246 mean_circ: 0.941381
Ran for 218550 frames Cell Events: 5269 mean_circ: 0.941381
Ran for 219050 frames Cell Events: 5281 mean_circ: 0.941362
Ran for 220550 frames Cell Events: 5317 mean_circ: 0.941317
Ran for 221050 frames Cell Events: 5333 mean_circ: 0.941357
Ran for 221550 frames Cell Events: 5349 mean_circ: 0.94142
Ran for 222050 frames Cell Events: 5355 mean_circ: 0.941436
Ran for 222550 frames Cell Events: 5373 mean_circ: 0.941446
Ran for 223050 frames Cell Events: 5384 mean_circ: 0.941438
Ran for 223550 frames Cell Events: 5392 mean_circ: 0.941429
Ran for 224050 frames Cell Events: 5408 mean_circ: 0.941443
Ran for 224550 frames Cell Events: 5417 mean_circ: 0.941471
Ran for 225050 frames Cell Events: 5440 mean_circ: 0.941506
Ran for 225550 frames Cell Events: 5446 mean_circ: 0.941504
Ran for 226050 frames Cell Events: 5462 mean_circ: 0.941484
Ran for 226550 frames Cell Events: 5472 mean_circ: 0.941505
Ran for 227050 frames Cell Events: 5494 mean_circ: 0.941517
Ran for 227550 frames Cell Events: 5505 mean_circ: 0.941549
Ran for 228550 frames Cell Events: 5531 mean_circ: 0.941552
Ran for 229050 frames Cell Events: 5545 mean_circ: 0.941531
Ran for 229550 frames Cell Events: 5559 mean_circ: 0.941578
Ran for 230050 frames Cell Events: 5573 mean_circ: 0.941571
Ran for 230550 frames Cell Events: 5586 mean_circ: 0.941573
Ran for 231050 frames Cell Events: 5594 mean_circ: 0.941568
Ran for 232050 frames Cell Events: 5623 mean_circ: 0.941561
Ran for 233050 frames Cell Events: 5651 mean_circ: 0.941531
Ran for 234050 frames Cell Events: 5684 mean_circ: 0.941569
Ran for 234550 frames Cell Events: 5702 mean_circ: 0.941588
Ran for 235050 frames Cell Events: 5715 mean_circ: 0.941588
Ran for 236050 frames Cell Events: 5731 mean_circ: 0.941588
Ran for 237050 frames Cell Events: 5752 mean_circ: 0.941578
Ran for 237550 frames Cell Events: 5769 mean_circ: 0.941556
Ran for 238050 frames Cell Events: 5780 mean_circ: 0.941556
Ran for 238550 frames Cell Events: 5792 mean_circ: 0.941555
Ran for 239050 frames Cell Events: 5802 mean_circ: 0.941554
Ran for 239550 frames Cell Events: 5815 mean_circ: 0.941589
Ran for 240050 frames Cell Events: 5826 mean_circ: 0.941569
Ran for 240550 frames Cell Events: 5839 mean_circ: 0.941524
Ran for 242050 frames Cell Events: 5890 mean_circ: 0.941474
Ran for 242550 frames Cell Events: 5906 mean_circ: 0.941483
Ran for 243050 frames Cell Events: 5924 mean_circ: 0.941456
Ran for 244050 frames Cell Events: 5949 mean_circ: 0.941471
Ran for 244550 frames Cell Events: 5955 mean_circ: 0.941469
Ran for 245050 frames Cell Events: 5964 mean_circ: 0.941509
Ran for 246550 frames Cell Events: 6004 mean_circ: 0.941579
Ran for 247550 frames Cell Events: 6030 mean_circ: 0.941524
Ran for 248050 frames Cell Events: 6041 mean_circ: 0.941556
Ran for 249050 frames Cell Events: 6077 mean_circ: 0.941599
Ran for 249550 frames Cell Events: 6084 mean_circ: 0.94161
Ran for 250050 frames Cell Events: 6113 mean_circ: 0.941627
Ran for 250550 frames Cell Events: 6124 mean_circ: 0.941655
Ran for 251050 frames Cell Events: 6140 mean_circ: 0.941659
Ran for 252050 frames Cell Events: 6165 mean_circ: 0.941703
Ran for 252550 frames Cell Events: 6179 mean_circ: 0.941659
Ran for 253550 frames Cell Events: 6188 mean_circ: 0.941676
Ran for 254050 frames Cell Events: 6197 mean_circ: 0.941683
Ran for 254550 frames Cell Events: 6207 mean_circ: 0.94169
Ran for 255050 frames Cell Events: 6224 mean_circ: 0.941702
Ran for 255550 frames Cell Events: 6237 mean_circ: 0.9417
Ran for 256550 frames Cell Events: 6264 mean_circ: 0.941713
Ran for 257050 frames Cell Events: 6278 mean_circ: 0.941729
Ran for 257550 frames Cell Events: 6285 mean_circ: 0.941732
Ran for 258050 frames Cell Events: 6300 mean_circ: 0.941746
Ran for 258550 frames Cell Events: 6311 mean_circ: 0.941774
Ran for 259050 frames Cell Events: 6321 mean_circ: 0.941763
Ran for 259550 frames Cell Events: 6336 mean_circ: 0.941778
Ran for 260550 frames Cell Events: 6367 mean_circ: 0.941794
Ran for 261050 frames Cell Events: 6378 mean_circ: 0.941811
Ran for 261550 frames Cell Events: 6394 mean_circ: 0.941833
Ran for 262550 frames Cell Events: 6426 mean_circ: 0.941813
Ran for 263050 frames Cell Events: 6444 mean_circ: 0.941742
Ran for 264050 frames Cell Events: 6467 mean_circ: 0.941775
Ran for 264550 frames Cell Events: 6480 mean_circ: 0.94175
Ran for 265050 frames Cell Events: 6488 mean_circ: 0.941756
Ran for 265550 frames Cell Events: 6502 mean_circ: 0.941777
Ran for 266050 frames Cell Events: 6511 mean_circ: 0.941792
Ran for 266550 frames Cell Events: 6522 mean_circ: 0.941782
Ran for 267050 frames Cell Events: 6532 mean_circ: 0.941786
Ran for 267550 frames Cell Events: 6540 mean_circ: 0.941759
Ran for 268050 frames Cell Events: 6563 mean_circ: 0.941759
Ran for 268550 frames Cell Events: 6570 mean_circ: 0.94176
Ran for 269550 frames Cell Events: 6598 mean_circ: 0.941737
Ran for 271050 frames Cell Events: 6648 mean_circ: 0.94171
Ran for 271550 frames Cell Events: 6670 mean_circ: 0.941762
Ran for 272550 frames Cell Events: 6693 mean_circ: 0.941747
Ran for 273550 frames Cell Events: 6706 mean_circ: 0.941715
Ran for 274050 frames Cell Events: 6723 mean_circ: 0.94168
Ran for 274550 frames Cell Events: 6745 mean_circ: 0.941658
Ran for 275050 frames Cell Events: 6765 mean_circ: 0.941686
Ran for 276050 frames Cell Events: 6796 mean_circ: 0.941741
Ran for 276550 frames Cell Events: 6803 mean_circ: 0.941749
Ran for 277550 frames Cell Events: 6836 mean_circ: 0.94179
Ran for 279050 frames Cell Events: 6878 mean_circ: 0.941816
Ran for 279550 frames Cell Events: 6895 mean_circ: 0.941853
Ran for 280550 frames Cell Events: 6922 mean_circ: 0.941821
Ran for 281050 frames Cell Events: 6930 mean_circ: 0.941817
Ran for 281550 frames Cell Events: 6944 mean_circ: 0.941804
Ran for 282050 frames Cell Events: 6958 mean_circ: 0.941807
Ran for 282550 frames Cell Events: 6969 mean_circ: 0.941816
Ran for 283050 frames Cell Events: 6983 mean_circ: 0.941829
Ran for 283550 frames Cell Events: 6999 mean_circ: 0.941855
Ran for 284050 frames Cell Events: 7005 mean_circ: 0.941859
Ran for 284550 frames Cell Events: 7014 mean_circ: 0.941856
Ran for 285050 frames Cell Events: 7018 mean_circ: 0.941858
Ran for 285550 frames Cell Events: 7028 mean_circ: 0.941839
Ran for 286050 frames Cell Events: 7049 mean_circ: 0.94186
Ran for 286550 frames Cell Events: 7062 mean_circ: 0.941899
Ran for 287050 frames Cell Events: 7083 mean_circ: 0.941884
Ran for 287550 frames Cell Events: 7091 mean_circ: 0.941906
Ran for 288050 frames Cell Events: 7103 mean_circ: 0.941862
Ran for 288550 frames Cell Events: 7124 mean_circ: 0.941897
Ran for 290050 frames Cell Events: 7170 mean_circ: 0.941837
Ran for 290550 frames Cell Events: 7180 mean_circ: 0.941844
Ran for 291050 frames Cell Events: 7192 mean_circ: 0.941856
Ran for 291550 frames Cell Events: 7206 mean_circ: 0.941859
Ran for 292050 frames Cell Events: 7213 mean_circ: 0.941865
Ran for 292550 frames Cell Events: 7223 mean_circ: 0.94188
Ran for 293550 frames Cell Events: 7264 mean_circ: 0.941865
Ran for 294050 frames Cell Events: 7278 mean_circ: 0.941889
Ran for 295050 frames Cell Events: 7293 mean_circ: 0.941908
Ran for 295550 frames Cell Events: 7305 mean_circ: 0.941867
Ran for 296050 frames Cell Events: 7313 mean_circ: 0.941849
Ran for 297050 frames Cell Events: 7335 mean_circ: 0.941864
Ran for 298050 frames Cell Events: 7350 mean_circ: 0.941881
Ran for 298550 frames Cell Events: 7367 mean_circ: 0.9419
Ran for 299050 frames Cell Events: 7378 mean_circ: 0.941888
Ran for 299550 frames Cell Events: 7388 mean_circ: 0.941865
Ran for 300050 frames Cell Events: 7399 mean_circ: 0.941895
Ran for 301050 frames Cell Events: 7412 mean_circ: 0.941891
Ran for 301550 frames Cell Events: 7428 mean_circ: 0.941906
Ran for 302050 frames Cell Events: 7438 mean_circ: 0.941922
Ran for 302550 frames Cell Events: 7448 mean_circ: 0.941906
Ran for 303050 frames Cell Events: 7458 mean_circ: 0.94188
Ran for 304050 frames Cell Events: 7499 mean_circ: 0.941922
Ran for 304550 frames Cell Events: 7508 mean_circ: 0.941931
Ran for 306050 frames Cell Events: 7551 mean_circ: 0.941989
Ran for 306550 frames Cell Events: 7565 mean_circ: 0.942001
Ran for 307050 frames Cell Events: 7575 mean_circ: 0.942003
Ran for 307550 frames Cell Events: 7591 mean_circ: 0.942005
Ran for 308550 frames Cell Events: 7627 mean_circ: 0.942072
Ran for 309050 frames Cell Events: 7644 mean_circ: 0.942104
Ran for 309550 frames Cell Events: 7662 mean_circ: 0.942128
Ran for 310050 frames Cell Events: 7670 mean_circ: 0.942136
Ran for 310550 frames Cell Events: 7684 mean_circ: 0.942134
Ran for 311550 frames Cell Events: 7715 mean_circ: 0.942133
Ran for 312050 frames Cell Events: 7729 mean_circ: 0.942132
Ran for 312550 frames Cell Events: 7755 mean_circ: 0.942126
Ran for 313550 frames Cell Events: 7785 mean_circ: 0.942143
Ran for 314050 frames Cell Events: 7798 mean_circ: 0.942157
Ran for 314550 frames Cell Events: 7815 mean_circ: 0.942198
Ran for 315050 frames Cell Events: 7828 mean_circ: 0.942183
Ran for 315550 frames Cell Events: 7839 mean_circ: 0.942168
Ran for 316050 frames Cell Events: 7853 mean_circ: 0.942175
Ran for 317050 frames Cell Events: 7888 mean_circ: 0.942186
Ran for 317550 frames Cell Events: 7901 mean_circ: 0.942196
Ran for 318050 frames Cell Events: 7907 mean_circ: 0.942148
Ran for 318550 frames Cell Events: 7928 mean_circ: 0.942162
Ran for 319550 frames Cell Events: 7956 mean_circ: 0.942163
Ran for 320550 frames Cell Events: 7973 mean_circ: 0.942112
Ran for 321050 frames Cell Events: 7985 mean_circ: 0.942118
Ran for 321550 frames Cell Events: 7993 mean_circ: 0.942134



End this measurment..



Stopping time: 5.52786e+008
****************************************
Measurement finished

Mean Circularitiy: 0.94213
Mean Area: 603.263
Mean FL-1 intensity: 2846.63
Mean FL-2 intensity: 3865.74
Mean FL-3 intensity: 0

frames processed: 317615
Total Time: 161366ms
Number of Runs (new bg img) 0
Frames collected: 322624
Skipped frames: 62822
Rescued Frames: 3001726
Samples DAQ: 80656065
Samples written: 161312048
Samples per Channel: 80656024
FPS: 1999.33

Samples per second: 499832
Expected samples/s: 500000

Events: 8025
Events per second: 49.7315
jumped back (PMT): 5652
#Frames read, frequency
# 0 frames :432505
# 1 frames :192909
# 2 frames :5958
# 3 frames :1903
# 4 frames :1067
# 5 frames :731
# 6 frames :1166
# 7 frames :2908
# 8 frames :1328
# 9 frames :879
# 10 frames :725
# 11 frames :661
# 12 frames :537
# 13 frames :430
# 14 frames :290
# 15 frames :246
# 16 frames :191
# 17 frames :171
# 18 frames :117
# 19 frames :82
# 20 frames :47
# 21 frames :54
# 22 frames :43
# 23 frames :33
# 24 frames :26
# 25 frames :32
# 26 frames :21
# 27 frames :18
# 28 frames :19
# 29 frames :20
# 30 frames :10
# 31 frames :7
# 32 frames :5
# 33 frames :6
# 34 frames :1
# 35 frames :4
# 36 frames :4
# 37 frames :9
# 38 frames :1
# 39 frames :2
# 40 frames :2
# 41 frames :1
# 42 frames :1
# 43 frames :1
# 46 frames :1
# 49 frames :2
# 51 frames :1
# 55 frames :1
# 60 frames :1
# 71 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.2005
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0