Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.11.35 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "0faf15a549d7385da410aa9a4468e8f6",
"name": "M1_20170603_1637_dsRNA_B1_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:11:55"
}
}
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.393
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.67128e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 0 mean_circ: 0.933633
Ran for 550 frames Cell Events: 12 mean_circ: 0.932623
Ran for 1550 frames Cell Events: 40 mean_circ: 0.920535
Ran for 2050 frames Cell Events: 69 mean_circ: 0.925902
Ran for 2550 frames Cell Events: 81 mean_circ: 0.928666
Ran for 3050 frames Cell Events: 96 mean_circ: 0.930105
Ran for 4050 frames Cell Events: 134 mean_circ: 0.930273
Ran for 4550 frames Cell Events: 161 mean_circ: 0.926993
Ran for 6050 frames Cell Events: 237 mean_circ: 0.926981
Ran for 6550 frames Cell Events: 253 mean_circ: 0.92705
Ran for 7050 frames Cell Events: 276 mean_circ: 0.926632
Ran for 7550 frames Cell Events: 298 mean_circ: 0.926277
Ran for 9550 frames Cell Events: 384 mean_circ: 0.927764
Ran for 11050 frames Cell Events: 455 mean_circ: 0.92785
Ran for 11550 frames Cell Events: 475 mean_circ: 0.927234
Ran for 12050 frames Cell Events: 495 mean_circ: 0.927385
Ran for 13050 frames Cell Events: 528 mean_circ: 0.92708
Ran for 13550 frames Cell Events: 545 mean_circ: 0.927388
Ran for 15550 frames Cell Events: 660 mean_circ: 0.92765
Ran for 16550 frames Cell Events: 703 mean_circ: 0.927942
Ran for 17050 frames Cell Events: 724 mean_circ: 0.92756
Ran for 18050 frames Cell Events: 760 mean_circ: 0.927304
Ran for 19050 frames Cell Events: 795 mean_circ: 0.927376
Ran for 19550 frames Cell Events: 821 mean_circ: 0.927122
Ran for 21550 frames Cell Events: 884 mean_circ: 0.927192
Ran for 22050 frames Cell Events: 907 mean_circ: 0.926795
Ran for 22550 frames Cell Events: 933 mean_circ: 0.926736
Ran for 24050 frames Cell Events: 1014 mean_circ: 0.926968
Ran for 24550 frames Cell Events: 1039 mean_circ: 0.926951
Ran for 25550 frames Cell Events: 1109 mean_circ: 0.927061
Ran for 26550 frames Cell Events: 1172 mean_circ: 0.927068
Ran for 27050 frames Cell Events: 1195 mean_circ: 0.927115
Ran for 27550 frames Cell Events: 1217 mean_circ: 0.92746
Ran for 28550 frames Cell Events: 1257 mean_circ: 0.927112
Ran for 29050 frames Cell Events: 1273 mean_circ: 0.927087
Ran for 29550 frames Cell Events: 1294 mean_circ: 0.927299
Ran for 30050 frames Cell Events: 1325 mean_circ: 0.927383
Ran for 31050 frames Cell Events: 1379 mean_circ: 0.927285
Ran for 34050 frames Cell Events: 1506 mean_circ: 0.927372
Ran for 35050 frames Cell Events: 1551 mean_circ: 0.927374
Ran for 36050 frames Cell Events: 1608 mean_circ: 0.927295
Ran for 36550 frames Cell Events: 1635 mean_circ: 0.927405
Ran for 37050 frames Cell Events: 1671 mean_circ: 0.927421
Ran for 37550 frames Cell Events: 1691 mean_circ: 0.927621
Ran for 38050 frames Cell Events: 1711 mean_circ: 0.927813
Ran for 38550 frames Cell Events: 1728 mean_circ: 0.927853
Ran for 39550 frames Cell Events: 1776 mean_circ: 0.927787
Ran for 40550 frames Cell Events: 1831 mean_circ: 0.92764
Ran for 43050 frames Cell Events: 1947 mean_circ: 0.927743
Ran for 43550 frames Cell Events: 1975 mean_circ: 0.927737
Ran for 44050 frames Cell Events: 1998 mean_circ: 0.927764
Ran for 45050 frames Cell Events: 2050 mean_circ: 0.927789
Ran for 46050 frames Cell Events: 2115 mean_circ: 0.927811
Ran for 46550 frames Cell Events: 2130 mean_circ: 0.927743
Ran for 48050 frames Cell Events: 2222 mean_circ: 0.927686
Ran for 49050 frames Cell Events: 2281 mean_circ: 0.927958
Ran for 50050 frames Cell Events: 2338 mean_circ: 0.927828
Ran for 50550 frames Cell Events: 2372 mean_circ: 0.927823
Ran for 52550 frames Cell Events: 2492 mean_circ: 0.927783
Ran for 55550 frames Cell Events: 2676 mean_circ: 0.927965
Ran for 56050 frames Cell Events: 2702 mean_circ: 0.928015
Ran for 59550 frames Cell Events: 2876 mean_circ: 0.928027
Ran for 60050 frames Cell Events: 2907 mean_circ: 0.927876
Ran for 62050 frames Cell Events: 3000 mean_circ: 0.927648
Ran for 62550 frames Cell Events: 3008 mean_circ: 0.927614
Ran for 63050 frames Cell Events: 3024 mean_circ: 0.92768
Ran for 64550 frames Cell Events: 3090 mean_circ: 0.927807
Ran for 66050 frames Cell Events: 3157 mean_circ: 0.927705
Ran for 66550 frames Cell Events: 3181 mean_circ: 0.927803
Ran for 67050 frames Cell Events: 3214 mean_circ: 0.927803
Ran for 67550 frames Cell Events: 3232 mean_circ: 0.927751
Ran for 69050 frames Cell Events: 3285 mean_circ: 0.927631
Ran for 69550 frames Cell Events: 3302 mean_circ: 0.927642
Ran for 70050 frames Cell Events: 3335 mean_circ: 0.927561
Ran for 71050 frames Cell Events: 3383 mean_circ: 0.92753
Ran for 72050 frames Cell Events: 3437 mean_circ: 0.927608
Ran for 72550 frames Cell Events: 3461 mean_circ: 0.927602
Ran for 74550 frames Cell Events: 3568 mean_circ: 0.927437
Ran for 77050 frames Cell Events: 3708 mean_circ: 0.927561
Ran for 77550 frames Cell Events: 3730 mean_circ: 0.927525
Ran for 78050 frames Cell Events: 3753 mean_circ: 0.927498
Ran for 79550 frames Cell Events: 3812 mean_circ: 0.927486
Ran for 80050 frames Cell Events: 3832 mean_circ: 0.927556
Ran for 81050 frames Cell Events: 3895 mean_circ: 0.927567
Ran for 81550 frames Cell Events: 3914 mean_circ: 0.927557
Ran for 83050 frames Cell Events: 3960 mean_circ: 0.927638
Ran for 84050 frames Cell Events: 4012 mean_circ: 0.92762
Ran for 85550 frames Cell Events: 4077 mean_circ: 0.927644
Ran for 88050 frames Cell Events: 4200 mean_circ: 0.927617
Ran for 88550 frames Cell Events: 4219 mean_circ: 0.927664
Ran for 89050 frames Cell Events: 4238 mean_circ: 0.927606
Ran for 90550 frames Cell Events: 4298 mean_circ: 0.927583
Ran for 91550 frames Cell Events: 4352 mean_circ: 0.927545
Ran for 92550 frames Cell Events: 4406 mean_circ: 0.927598
Ran for 93050 frames Cell Events: 4427 mean_circ: 0.927554
Ran for 93550 frames Cell Events: 4450 mean_circ: 0.927571
Ran for 95550 frames Cell Events: 4552 mean_circ: 0.927626
Ran for 97050 frames Cell Events: 4629 mean_circ: 0.927532
Ran for 98050 frames Cell Events: 4671 mean_circ: 0.927586
Ran for 99050 frames Cell Events: 4714 mean_circ: 0.927589
Ran for 100550 frames Cell Events: 4814 mean_circ: 0.927636
Ran for 101550 frames Cell Events: 4880 mean_circ: 0.927699
Ran for 102050 frames Cell Events: 4907 mean_circ: 0.927652
Ran for 103050 frames Cell Events: 4963 mean_circ: 0.927661
Ran for 103550 frames Cell Events: 4987 mean_circ: 0.927573
Ran for 104550 frames Cell Events: 5025 mean_circ: 0.927588
Ran for 106550 frames Cell Events: 5138 mean_circ: 0.927439
Ran for 107050 frames Cell Events: 5159 mean_circ: 0.927448
Ran for 108550 frames Cell Events: 5260 mean_circ: 0.927309
Ran for 111050 frames Cell Events: 5404 mean_circ: 0.92738
Ran for 111550 frames Cell Events: 5429 mean_circ: 0.927412
Ran for 113050 frames Cell Events: 5502 mean_circ: 0.927383
Ran for 113550 frames Cell Events: 5527 mean_circ: 0.927299
Ran for 115050 frames Cell Events: 5625 mean_circ: 0.927342
Ran for 115550 frames Cell Events: 5660 mean_circ: 0.927369
Ran for 116550 frames Cell Events: 5715 mean_circ: 0.927265
Ran for 117550 frames Cell Events: 5780 mean_circ: 0.9272
Ran for 118050 frames Cell Events: 5808 mean_circ: 0.927179
Ran for 119050 frames Cell Events: 5867 mean_circ: 0.927162
Ran for 119550 frames Cell Events: 5900 mean_circ: 0.927119
Ran for 122050 frames Cell Events: 6045 mean_circ: 0.927016
Ran for 122550 frames Cell Events: 6075 mean_circ: 0.927016
Ran for 124550 frames Cell Events: 6177 mean_circ: 0.926849
Ran for 126550 frames Cell Events: 6276 mean_circ: 0.926787
Ran for 128050 frames Cell Events: 6366 mean_circ: 0.92677
Ran for 128550 frames Cell Events: 6387 mean_circ: 0.926781
Ran for 129050 frames Cell Events: 6400 mean_circ: 0.926757
Ran for 130550 frames Cell Events: 6508 mean_circ: 0.926694
Ran for 131050 frames Cell Events: 6533 mean_circ: 0.926765
Ran for 132050 frames Cell Events: 6581 mean_circ: 0.926796
Ran for 132550 frames Cell Events: 6605 mean_circ: 0.926788
Ran for 133550 frames Cell Events: 6656 mean_circ: 0.926768
Ran for 135550 frames Cell Events: 6758 mean_circ: 0.926731
Ran for 136050 frames Cell Events: 6791 mean_circ: 0.926747
Ran for 137050 frames Cell Events: 6835 mean_circ: 0.92678
Ran for 138550 frames Cell Events: 6920 mean_circ: 0.926619
Ran for 140050 frames Cell Events: 6989 mean_circ: 0.926623
Ran for 140550 frames Cell Events: 7017 mean_circ: 0.926555
Ran for 141050 frames Cell Events: 7031 mean_circ: 0.92658
Ran for 143050 frames Cell Events: 7128 mean_circ: 0.926535
Ran for 143550 frames Cell Events: 7146 mean_circ: 0.926498
Ran for 144050 frames Cell Events: 7162 mean_circ: 0.926444
Ran for 144550 frames Cell Events: 7176 mean_circ: 0.926456
Ran for 145050 frames Cell Events: 7195 mean_circ: 0.926438
Ran for 145550 frames Cell Events: 7216 mean_circ: 0.926438
Ran for 148550 frames Cell Events: 7360 mean_circ: 0.926396
Ran for 149550 frames Cell Events: 7414 mean_circ: 0.926392
Ran for 150050 frames Cell Events: 7431 mean_circ: 0.926362
Ran for 150550 frames Cell Events: 7444 mean_circ: 0.926386
Ran for 151550 frames Cell Events: 7500 mean_circ: 0.926287
Ran for 152050 frames Cell Events: 7530 mean_circ: 0.926339
Ran for 152550 frames Cell Events: 7555 mean_circ: 0.926347
Ran for 153050 frames Cell Events: 7581 mean_circ: 0.926326
Ran for 154550 frames Cell Events: 7660 mean_circ: 0.926367
Ran for 155550 frames Cell Events: 7708 mean_circ: 0.926333
Ran for 156550 frames Cell Events: 7758 mean_circ: 0.92626
Ran for 157050 frames Cell Events: 7784 mean_circ: 0.926232
Ran for 157550 frames Cell Events: 7817 mean_circ: 0.926207
Ran for 159550 frames Cell Events: 7897 mean_circ: 0.926124
Ran for 160050 frames Cell Events: 7925 mean_circ: 0.926124
Ran for 161050 frames Cell Events: 7984 mean_circ: 0.926167
Ran for 161550 frames Cell Events: 7999 mean_circ: 0.926172
Ran for 162050 frames Cell Events: 8021 mean_circ: 0.926228
Ran for 162550 frames Cell Events: 8040 mean_circ: 0.926214
Ran for 163050 frames Cell Events: 8059 mean_circ: 0.926216



End this measurment..



Stopping time: 1.67136e+009
****************************************
Measurement finished

Mean Circularitiy: 0.926219
Mean Area: 719.476
Mean FL-1 intensity: 4126.15
Mean FL-2 intensity: 4289.87
Mean FL-3 intensity: 0

frames processed: 162513
Total Time: 81752.3ms
Number of Runs (new bg img) 0
Frames collected: 163355
Skipped frames: 58701
Rescued Frames: 1853783
Samples DAQ: 40840868
Samples written: 81677298
Samples per Channel: 40838649
FPS: 1998.17

Samples per second: 499541
Expected samples/s: 500000

Events: 8076
Events per second: 98.7862
jumped back (PMT): 3436
#Frames read, frequency
# 0 frames :253521
# 1 frames :59453
# 2 frames :3354
# 3 frames :1172
# 4 frames :683
# 5 frames :494
# 6 frames :942
# 7 frames :1504
# 8 frames :928
# 9 frames :609
# 10 frames :705
# 11 frames :573
# 12 frames :398
# 13 frames :231
# 14 frames :193
# 15 frames :214
# 16 frames :224
# 17 frames :167
# 18 frames :171
# 19 frames :128
# 20 frames :83
# 21 frames :87
# 22 frames :72
# 23 frames :88
# 24 frames :48
# 25 frames :54
# 26 frames :36
# 27 frames :41
# 28 frames :24
# 29 frames :22
# 30 frames :19
# 31 frames :22
# 32 frames :15
# 33 frames :20
# 34 frames :16
# 35 frames :12
# 36 frames :16
# 37 frames :9
# 38 frames :12
# 39 frames :6
# 40 frames :5
# 41 frames :3
# 42 frames :7
# 43 frames :7
# 44 frames :2
# 45 frames :3
# 46 frames :2
# 47 frames :2
# 48 frames :4
# 49 frames :2
# 50 frames :1
# 51 frames :1
# 52 frames :3
# 53 frames :1
# 54 frames :3
# 55 frames :1
# 57 frames :2
# 59 frames :1
# 61 frames :1
# 62 frames :1
# 69 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.393
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0