Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.14.23 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "739829ae0d0b002e78b3721e931082c8",
"name": "M1_20170603_1708_dsRNA_b8_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:14:48"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 10000 (max 10001) in
'10000'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.4459
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.67315e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1550 frames Cell Events: 87 mean_circ: 0.941897
Ran for 2550 frames Cell Events: 135 mean_circ: 0.944273
Ran for 3050 frames Cell Events: 169 mean_circ: 0.94398
Ran for 3550 frames Cell Events: 188 mean_circ: 0.944236
Ran for 4550 frames Cell Events: 245 mean_circ: 0.944873
Ran for 5050 frames Cell Events: 279 mean_circ: 0.943524
Ran for 5550 frames Cell Events: 296 mean_circ: 0.944784
Ran for 6050 frames Cell Events: 320 mean_circ: 0.94532
Ran for 6550 frames Cell Events: 347 mean_circ: 0.945619
Ran for 7550 frames Cell Events: 387 mean_circ: 0.946315
Ran for 8050 frames Cell Events: 400 mean_circ: 0.946302
Ran for 10050 frames Cell Events: 498 mean_circ: 0.9459
Ran for 11050 frames Cell Events: 561 mean_circ: 0.945883
Ran for 12050 frames Cell Events: 609 mean_circ: 0.945092
Ran for 12550 frames Cell Events: 632 mean_circ: 0.945291
Ran for 13050 frames Cell Events: 667 mean_circ: 0.945598
Ran for 13550 frames Cell Events: 694 mean_circ: 0.945157
Ran for 14050 frames Cell Events: 718 mean_circ: 0.945407
Ran for 14550 frames Cell Events: 748 mean_circ: 0.945298
Ran for 16050 frames Cell Events: 829 mean_circ: 0.945308
Ran for 16550 frames Cell Events: 863 mean_circ: 0.945235
Ran for 17050 frames Cell Events: 905 mean_circ: 0.944944
Ran for 17550 frames Cell Events: 923 mean_circ: 0.945076
Ran for 18050 frames Cell Events: 940 mean_circ: 0.945099
Ran for 18550 frames Cell Events: 958 mean_circ: 0.944971
Ran for 20050 frames Cell Events: 1027 mean_circ: 0.944945
Ran for 20550 frames Cell Events: 1051 mean_circ: 0.945049
Ran for 22550 frames Cell Events: 1157 mean_circ: 0.945438
Ran for 23550 frames Cell Events: 1202 mean_circ: 0.945652
Ran for 24050 frames Cell Events: 1232 mean_circ: 0.945731
Ran for 25050 frames Cell Events: 1281 mean_circ: 0.945741
Ran for 25550 frames Cell Events: 1307 mean_circ: 0.945582
Ran for 26550 frames Cell Events: 1354 mean_circ: 0.945758
Ran for 28550 frames Cell Events: 1490 mean_circ: 0.945928
Ran for 29550 frames Cell Events: 1546 mean_circ: 0.946089
Ran for 30050 frames Cell Events: 1574 mean_circ: 0.946034
Ran for 30550 frames Cell Events: 1599 mean_circ: 0.94609
Ran for 31050 frames Cell Events: 1615 mean_circ: 0.946056
Ran for 32550 frames Cell Events: 1686 mean_circ: 0.946202
Ran for 34550 frames Cell Events: 1788 mean_circ: 0.946211
Ran for 35050 frames Cell Events: 1809 mean_circ: 0.946211
Ran for 36050 frames Cell Events: 1862 mean_circ: 0.946114
Ran for 40550 frames Cell Events: 2193 mean_circ: 0.94607
Ran for 41050 frames Cell Events: 2223 mean_circ: 0.946075
Ran for 42050 frames Cell Events: 2292 mean_circ: 0.946414
Ran for 42550 frames Cell Events: 2322 mean_circ: 0.946348
Ran for 43550 frames Cell Events: 2388 mean_circ: 0.946186
Ran for 44550 frames Cell Events: 2451 mean_circ: 0.946384
Ran for 45050 frames Cell Events: 2484 mean_circ: 0.946404
Ran for 45550 frames Cell Events: 2516 mean_circ: 0.9464
Ran for 46050 frames Cell Events: 2538 mean_circ: 0.946235
Ran for 46550 frames Cell Events: 2564 mean_circ: 0.946311
Ran for 47050 frames Cell Events: 2596 mean_circ: 0.946324
Ran for 48050 frames Cell Events: 2653 mean_circ: 0.9464
Ran for 49050 frames Cell Events: 2721 mean_circ: 0.946463
Ran for 49550 frames Cell Events: 2742 mean_circ: 0.946484
Ran for 50050 frames Cell Events: 2773 mean_circ: 0.946357
Ran for 50550 frames Cell Events: 2789 mean_circ: 0.946385
Ran for 51050 frames Cell Events: 2814 mean_circ: 0.946224
Ran for 52050 frames Cell Events: 2864 mean_circ: 0.946204
Ran for 52550 frames Cell Events: 2892 mean_circ: 0.946268
Ran for 54550 frames Cell Events: 3005 mean_circ: 0.946257
Ran for 55550 frames Cell Events: 3072 mean_circ: 0.946446
Ran for 56550 frames Cell Events: 3111 mean_circ: 0.94638
Ran for 57550 frames Cell Events: 3166 mean_circ: 0.946375
Ran for 58550 frames Cell Events: 3231 mean_circ: 0.946278
Ran for 59550 frames Cell Events: 3279 mean_circ: 0.946358
Ran for 60050 frames Cell Events: 3291 mean_circ: 0.946422
Ran for 60550 frames Cell Events: 3320 mean_circ: 0.946432
Ran for 61050 frames Cell Events: 3335 mean_circ: 0.946385
Ran for 61550 frames Cell Events: 3362 mean_circ: 0.946372
Ran for 62550 frames Cell Events: 3427 mean_circ: 0.946405
Ran for 64050 frames Cell Events: 3524 mean_circ: 0.9464
Ran for 65050 frames Cell Events: 3572 mean_circ: 0.946418
Ran for 66050 frames Cell Events: 3640 mean_circ: 0.946469
Ran for 67050 frames Cell Events: 3697 mean_circ: 0.946567
Ran for 67550 frames Cell Events: 3716 mean_circ: 0.946582
Ran for 68050 frames Cell Events: 3738 mean_circ: 0.946594
Ran for 68550 frames Cell Events: 3776 mean_circ: 0.946651
Ran for 69550 frames Cell Events: 3831 mean_circ: 0.946693
Ran for 71550 frames Cell Events: 3921 mean_circ: 0.946704
Ran for 72550 frames Cell Events: 3971 mean_circ: 0.946728
Ran for 73050 frames Cell Events: 4006 mean_circ: 0.946742
Ran for 73550 frames Cell Events: 4039 mean_circ: 0.946759
Ran for 74050 frames Cell Events: 4063 mean_circ: 0.946745
Ran for 75550 frames Cell Events: 4155 mean_circ: 0.946803
Ran for 77050 frames Cell Events: 4229 mean_circ: 0.946806
Ran for 77550 frames Cell Events: 4258 mean_circ: 0.946736
Ran for 78050 frames Cell Events: 4300 mean_circ: 0.946791
Ran for 79050 frames Cell Events: 4356 mean_circ: 0.946847
Ran for 79550 frames Cell Events: 4387 mean_circ: 0.946864
Ran for 80050 frames Cell Events: 4416 mean_circ: 0.946868
Ran for 80550 frames Cell Events: 4442 mean_circ: 0.946905
Ran for 81050 frames Cell Events: 4466 mean_circ: 0.9469
Ran for 81550 frames Cell Events: 4486 mean_circ: 0.9469
Ran for 82550 frames Cell Events: 4527 mean_circ: 0.946946
Ran for 83050 frames Cell Events: 4563 mean_circ: 0.947004
Ran for 85050 frames Cell Events: 4684 mean_circ: 0.946767
Ran for 85550 frames Cell Events: 4710 mean_circ: 0.946789
Ran for 87050 frames Cell Events: 4827 mean_circ: 0.946748
Ran for 88050 frames Cell Events: 4886 mean_circ: 0.946671
Ran for 89050 frames Cell Events: 4950 mean_circ: 0.946639
Ran for 90550 frames Cell Events: 5057 mean_circ: 0.946715
Ran for 94550 frames Cell Events: 5325 mean_circ: 0.946686
Ran for 96050 frames Cell Events: 5401 mean_circ: 0.946805
Ran for 97550 frames Cell Events: 5481 mean_circ: 0.946662
Ran for 100050 frames Cell Events: 5635 mean_circ: 0.946583
Ran for 100550 frames Cell Events: 5657 mean_circ: 0.946612
Ran for 101050 frames Cell Events: 5683 mean_circ: 0.946558
Ran for 103550 frames Cell Events: 5862 mean_circ: 0.946484
Ran for 104550 frames Cell Events: 5918 mean_circ: 0.94642
Ran for 107550 frames Cell Events: 6064 mean_circ: 0.946387
Ran for 108050 frames Cell Events: 6085 mean_circ: 0.946388
Ran for 108550 frames Cell Events: 6124 mean_circ: 0.946408
Ran for 109550 frames Cell Events: 6182 mean_circ: 0.946425
Ran for 110050 frames Cell Events: 6208 mean_circ: 0.94644
Ran for 111550 frames Cell Events: 6296 mean_circ: 0.946407
Ran for 113050 frames Cell Events: 6409 mean_circ: 0.946366
Ran for 114050 frames Cell Events: 6465 mean_circ: 0.946386
Ran for 116550 frames Cell Events: 6640 mean_circ: 0.94634
Ran for 118550 frames Cell Events: 6742 mean_circ: 0.946348
Ran for 119050 frames Cell Events: 6772 mean_circ: 0.946369
Ran for 119550 frames Cell Events: 6797 mean_circ: 0.946386
Ran for 120050 frames Cell Events: 6817 mean_circ: 0.946372
Ran for 120550 frames Cell Events: 6835 mean_circ: 0.946341
Ran for 121050 frames Cell Events: 6872 mean_circ: 0.946299
Ran for 121550 frames Cell Events: 6911 mean_circ: 0.946327
Ran for 122050 frames Cell Events: 6944 mean_circ: 0.946333
Ran for 128550 frames Cell Events: 7341 mean_circ: 0.946273
Ran for 129050 frames Cell Events: 7370 mean_circ: 0.946285
Ran for 130050 frames Cell Events: 7437 mean_circ: 0.946219
Ran for 131050 frames Cell Events: 7496 mean_circ: 0.94623
Ran for 132050 frames Cell Events: 7552 mean_circ: 0.946233
Ran for 132550 frames Cell Events: 7577 mean_circ: 0.946246
Ran for 134550 frames Cell Events: 7717 mean_circ: 0.946247
Ran for 135050 frames Cell Events: 7750 mean_circ: 0.946218
Ran for 135550 frames Cell Events: 7789 mean_circ: 0.946222
Ran for 140050 frames Cell Events: 8020 mean_circ: 0.946298
Ran for 140550 frames Cell Events: 8048 mean_circ: 0.946325
Ran for 142050 frames Cell Events: 8139 mean_circ: 0.946384
Ran for 142550 frames Cell Events: 8161 mean_circ: 0.946399
Ran for 144050 frames Cell Events: 8274 mean_circ: 0.946369
Ran for 144550 frames Cell Events: 8298 mean_circ: 0.946376
Ran for 145050 frames Cell Events: 8330 mean_circ: 0.946353
Ran for 148050 frames Cell Events: 8495 mean_circ: 0.946356
Ran for 148550 frames Cell Events: 8520 mean_circ: 0.946368
Ran for 149050 frames Cell Events: 8555 mean_circ: 0.94639
Ran for 150550 frames Cell Events: 8647 mean_circ: 0.946379
Ran for 151050 frames Cell Events: 8675 mean_circ: 0.946372
Ran for 151550 frames Cell Events: 8699 mean_circ: 0.946341
Ran for 152550 frames Cell Events: 8761 mean_circ: 0.946349
Ran for 157050 frames Cell Events: 9058 mean_circ: 0.946347
Ran for 158050 frames Cell Events: 9111 mean_circ: 0.946353
Ran for 158550 frames Cell Events: 9129 mean_circ: 0.946362
Ran for 159050 frames Cell Events: 9152 mean_circ: 0.946359
Ran for 160050 frames Cell Events: 9202 mean_circ: 0.946344
Ran for 160550 frames Cell Events: 9232 mean_circ: 0.946334
Ran for 161550 frames Cell Events: 9307 mean_circ: 0.946348
Ran for 162050 frames Cell Events: 9331 mean_circ: 0.946319
Ran for 163050 frames Cell Events: 9383 mean_circ: 0.946275
Ran for 163550 frames Cell Events: 9411 mean_circ: 0.946254
Ran for 164050 frames Cell Events: 9432 mean_circ: 0.946268
Ran for 166050 frames Cell Events: 9554 mean_circ: 0.946251
Ran for 168550 frames Cell Events: 9739 mean_circ: 0.946187
Ran for 169050 frames Cell Events: 9754 mean_circ: 0.946183



End this measurment.
because stop condition (time/nr of cells) was met

Stopping time: 1.67323e+009
****************************************
Measurement finished

Mean Circularitiy: 0.946265
Mean Area: 652.945
Mean FL-1 intensity: 3964.92
Mean FL-2 intensity: 3106.35
Mean FL-3 intensity: 0

frames processed: 171052
Total Time: 86457.1ms
Number of Runs (new bg img) 0
Frames collected: 172787
Skipped frames: 81817
Rescued Frames: 2345545
Samples DAQ: 43196788
Samples written: 86393252
Samples per Channel: 43196626
FPS: 1998.53

Samples per second: 499631
Expected samples/s: 500000

Events: 10001
Events per second: 115.676
jumped back (PMT): 3837
#Frames read, frequency
# 0 frames :276915
# 1 frames :53966
# 2 frames :3237
# 3 frames :1337
# 4 frames :829
# 5 frames :460
# 6 frames :1308
# 7 frames :1350
# 8 frames :955
# 9 frames :703
# 10 frames :824
# 11 frames :656
# 12 frames :419
# 13 frames :276
# 14 frames :268
# 15 frames :251
# 16 frames :231
# 17 frames :208
# 18 frames :157
# 19 frames :126
# 20 frames :114
# 21 frames :88
# 22 frames :85
# 23 frames :69
# 24 frames :60
# 25 frames :69
# 26 frames :51
# 27 frames :38
# 28 frames :37
# 29 frames :39
# 30 frames :48
# 31 frames :36
# 32 frames :24
# 33 frames :21
# 34 frames :14
# 35 frames :11
# 36 frames :19
# 37 frames :18
# 38 frames :18
# 39 frames :10
# 40 frames :4
# 41 frames :9
# 42 frames :14
# 43 frames :7
# 44 frames :5
# 45 frames :6
# 46 frames :4
# 47 frames :4
# 48 frames :8
# 49 frames :6
# 50 frames :3
# 51 frames :3
# 52 frames :3
# 53 frames :3
# 54 frames :1
# 56 frames :3
# 58 frames :3
# 60 frames :1
# 62 frames :1
# 67 frames :1
# 76 frames :1
# 79 frames :2
# 85 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.4459
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0