Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.17.17 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "2f77449c0eb79a03b4896ae519487e21",
"name": "M1_20170603_1745_dsRNA_C7_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:17:40"
}
}
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.4619
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.67535e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 0 mean_circ: 0.935398
Ran for 550 frames Cell Events: 9 mean_circ: 0.95111
Ran for 1050 frames Cell Events: 32 mean_circ: 0.945874
Ran for 1550 frames Cell Events: 54 mean_circ: 0.953383
Ran for 3050 frames Cell Events: 113 mean_circ: 0.955897
Ran for 4550 frames Cell Events: 189 mean_circ: 0.954134
Ran for 5050 frames Cell Events: 207 mean_circ: 0.953205
Ran for 5550 frames Cell Events: 230 mean_circ: 0.953006
Ran for 6050 frames Cell Events: 252 mean_circ: 0.950805
Ran for 6550 frames Cell Events: 266 mean_circ: 0.950496
Ran for 7050 frames Cell Events: 273 mean_circ: 0.950769
Ran for 7550 frames Cell Events: 290 mean_circ: 0.950109
Ran for 8050 frames Cell Events: 309 mean_circ: 0.950618
Ran for 8550 frames Cell Events: 315 mean_circ: 0.950724
Ran for 9550 frames Cell Events: 356 mean_circ: 0.95115
Ran for 10550 frames Cell Events: 393 mean_circ: 0.95181
Ran for 11050 frames Cell Events: 414 mean_circ: 0.95181
Ran for 14050 frames Cell Events: 549 mean_circ: 0.950176
Ran for 15050 frames Cell Events: 586 mean_circ: 0.949753
Ran for 15550 frames Cell Events: 607 mean_circ: 0.94993
Ran for 16050 frames Cell Events: 632 mean_circ: 0.949724
Ran for 16550 frames Cell Events: 654 mean_circ: 0.949836
Ran for 17050 frames Cell Events: 673 mean_circ: 0.949886
Ran for 17550 frames Cell Events: 691 mean_circ: 0.94988
Ran for 19550 frames Cell Events: 767 mean_circ: 0.950344
Ran for 20050 frames Cell Events: 781 mean_circ: 0.950412
Ran for 20550 frames Cell Events: 800 mean_circ: 0.95065
Ran for 21550 frames Cell Events: 832 mean_circ: 0.950681
Ran for 22050 frames Cell Events: 851 mean_circ: 0.950898
Ran for 22550 frames Cell Events: 868 mean_circ: 0.950877
Ran for 23050 frames Cell Events: 894 mean_circ: 0.950877
Ran for 24550 frames Cell Events: 948 mean_circ: 0.950304
Ran for 27050 frames Cell Events: 1054 mean_circ: 0.950508
Ran for 28550 frames Cell Events: 1123 mean_circ: 0.950423
Ran for 29550 frames Cell Events: 1162 mean_circ: 0.950522
Ran for 30550 frames Cell Events: 1190 mean_circ: 0.950611
Ran for 32050 frames Cell Events: 1251 mean_circ: 0.95042
Ran for 33050 frames Cell Events: 1284 mean_circ: 0.950555
Ran for 33550 frames Cell Events: 1298 mean_circ: 0.950586
Ran for 35050 frames Cell Events: 1357 mean_circ: 0.950614
Ran for 35550 frames Cell Events: 1378 mean_circ: 0.950628
Ran for 37050 frames Cell Events: 1430 mean_circ: 0.950295
Ran for 38550 frames Cell Events: 1493 mean_circ: 0.95024
Ran for 39050 frames Cell Events: 1502 mean_circ: 0.95023
Ran for 40050 frames Cell Events: 1535 mean_circ: 0.949974
Ran for 40550 frames Cell Events: 1559 mean_circ: 0.949956
Ran for 41050 frames Cell Events: 1575 mean_circ: 0.949966
Ran for 41550 frames Cell Events: 1603 mean_circ: 0.950042
Ran for 43050 frames Cell Events: 1671 mean_circ: 0.950367
Ran for 44050 frames Cell Events: 1703 mean_circ: 0.950471
Ran for 45050 frames Cell Events: 1743 mean_circ: 0.950496
Ran for 46550 frames Cell Events: 1804 mean_circ: 0.950506
Ran for 47050 frames Cell Events: 1823 mean_circ: 0.95044
Ran for 47550 frames Cell Events: 1846 mean_circ: 0.950456
Ran for 48050 frames Cell Events: 1865 mean_circ: 0.950545
Ran for 48550 frames Cell Events: 1882 mean_circ: 0.950575
Ran for 49550 frames Cell Events: 1933 mean_circ: 0.950466
Ran for 50050 frames Cell Events: 1951 mean_circ: 0.950539
Ran for 51050 frames Cell Events: 1991 mean_circ: 0.950563
Ran for 52050 frames Cell Events: 2035 mean_circ: 0.9506
Ran for 52550 frames Cell Events: 2045 mean_circ: 0.950615
Ran for 53050 frames Cell Events: 2063 mean_circ: 0.950634
Ran for 53550 frames Cell Events: 2089 mean_circ: 0.950539
Ran for 54050 frames Cell Events: 2108 mean_circ: 0.950524
Ran for 56050 frames Cell Events: 2194 mean_circ: 0.95054
Ran for 56550 frames Cell Events: 2220 mean_circ: 0.950518
Ran for 58550 frames Cell Events: 2319 mean_circ: 0.950411
Ran for 59050 frames Cell Events: 2332 mean_circ: 0.950386
Ran for 61550 frames Cell Events: 2442 mean_circ: 0.950284
Ran for 62050 frames Cell Events: 2461 mean_circ: 0.950245
Ran for 63050 frames Cell Events: 2508 mean_circ: 0.950201
Ran for 63550 frames Cell Events: 2537 mean_circ: 0.950136
Ran for 65050 frames Cell Events: 2630 mean_circ: 0.949978
Ran for 68050 frames Cell Events: 2772 mean_circ: 0.950084
Ran for 68550 frames Cell Events: 2791 mean_circ: 0.950128
Ran for 69050 frames Cell Events: 2817 mean_circ: 0.950221
Ran for 70050 frames Cell Events: 2865 mean_circ: 0.9502
Ran for 70550 frames Cell Events: 2885 mean_circ: 0.950183
Ran for 71050 frames Cell Events: 2908 mean_circ: 0.950224
Ran for 71550 frames Cell Events: 2917 mean_circ: 0.9501
Ran for 72050 frames Cell Events: 2938 mean_circ: 0.950149
Ran for 72550 frames Cell Events: 2960 mean_circ: 0.950212
Ran for 73050 frames Cell Events: 2977 mean_circ: 0.950175
Ran for 73550 frames Cell Events: 3005 mean_circ: 0.950183
Ran for 74050 frames Cell Events: 3028 mean_circ: 0.950333
Ran for 74550 frames Cell Events: 3036 mean_circ: 0.950288
Ran for 75050 frames Cell Events: 3067 mean_circ: 0.950383
Ran for 75550 frames Cell Events: 3090 mean_circ: 0.950344
Ran for 76050 frames Cell Events: 3110 mean_circ: 0.950392
Ran for 76550 frames Cell Events: 3128 mean_circ: 0.950377
Ran for 77050 frames Cell Events: 3147 mean_circ: 0.950398
Ran for 78550 frames Cell Events: 3209 mean_circ: 0.950431
Ran for 79550 frames Cell Events: 3255 mean_circ: 0.950485
Ran for 81050 frames Cell Events: 3311 mean_circ: 0.950428
Ran for 82550 frames Cell Events: 3368 mean_circ: 0.950257
Ran for 84550 frames Cell Events: 3479 mean_circ: 0.950188
Ran for 85050 frames Cell Events: 3504 mean_circ: 0.950188
Ran for 85550 frames Cell Events: 3527 mean_circ: 0.950161
Ran for 87050 frames Cell Events: 3597 mean_circ: 0.950274
Ran for 88550 frames Cell Events: 3665 mean_circ: 0.950217
Ran for 89550 frames Cell Events: 3717 mean_circ: 0.95025
Ran for 90050 frames Cell Events: 3737 mean_circ: 0.950226
Ran for 90550 frames Cell Events: 3754 mean_circ: 0.950266
Ran for 91050 frames Cell Events: 3775 mean_circ: 0.950333
Ran for 92050 frames Cell Events: 3813 mean_circ: 0.950378
Ran for 92550 frames Cell Events: 3837 mean_circ: 0.95025
Ran for 94550 frames Cell Events: 3951 mean_circ: 0.950166
Ran for 96050 frames Cell Events: 4014 mean_circ: 0.950219
Ran for 97550 frames Cell Events: 4080 mean_circ: 0.950297
Ran for 98550 frames Cell Events: 4131 mean_circ: 0.950351
Ran for 100050 frames Cell Events: 4186 mean_circ: 0.950408
Ran for 100550 frames Cell Events: 4211 mean_circ: 0.950366
Ran for 101050 frames Cell Events: 4236 mean_circ: 0.950348
Ran for 102050 frames Cell Events: 4284 mean_circ: 0.950397
Ran for 102550 frames Cell Events: 4310 mean_circ: 0.950427
Ran for 103050 frames Cell Events: 4329 mean_circ: 0.950422
Ran for 105050 frames Cell Events: 4417 mean_circ: 0.950463
Ran for 106050 frames Cell Events: 4466 mean_circ: 0.950576
Ran for 107050 frames Cell Events: 4515 mean_circ: 0.950549
Ran for 107550 frames Cell Events: 4531 mean_circ: 0.950573
Ran for 108550 frames Cell Events: 4568 mean_circ: 0.950618
Ran for 109050 frames Cell Events: 4588 mean_circ: 0.950615
Ran for 109550 frames Cell Events: 4611 mean_circ: 0.95059
Ran for 110050 frames Cell Events: 4628 mean_circ: 0.950603
Ran for 110550 frames Cell Events: 4650 mean_circ: 0.950586
Ran for 111050 frames Cell Events: 4671 mean_circ: 0.950575
Ran for 111550 frames Cell Events: 4684 mean_circ: 0.950571
Ran for 112050 frames Cell Events: 4701 mean_circ: 0.950559
Ran for 113050 frames Cell Events: 4757 mean_circ: 0.950609
Ran for 113550 frames Cell Events: 4782 mean_circ: 0.950601
Ran for 114050 frames Cell Events: 4809 mean_circ: 0.950507
Ran for 116050 frames Cell Events: 4915 mean_circ: 0.950474
Ran for 118550 frames Cell Events: 5060 mean_circ: 0.950393
Ran for 120550 frames Cell Events: 5133 mean_circ: 0.950354
Ran for 121050 frames Cell Events: 5155 mean_circ: 0.950358
Ran for 121550 frames Cell Events: 5172 mean_circ: 0.950384
Ran for 122550 frames Cell Events: 5218 mean_circ: 0.950412
Ran for 123050 frames Cell Events: 5237 mean_circ: 0.950448
Ran for 126550 frames Cell Events: 5391 mean_circ: 0.950431
Ran for 127050 frames Cell Events: 5419 mean_circ: 0.95046
Ran for 128050 frames Cell Events: 5459 mean_circ: 0.95049
Ran for 129050 frames Cell Events: 5502 mean_circ: 0.950506
Ran for 129550 frames Cell Events: 5519 mean_circ: 0.950453
Ran for 131050 frames Cell Events: 5580 mean_circ: 0.950454
Ran for 132050 frames Cell Events: 5616 mean_circ: 0.950453
Ran for 132550 frames Cell Events: 5630 mean_circ: 0.95047
Ran for 133550 frames Cell Events: 5680 mean_circ: 0.95048
Ran for 134050 frames Cell Events: 5699 mean_circ: 0.950493
Ran for 135050 frames Cell Events: 5735 mean_circ: 0.950504
Ran for 137050 frames Cell Events: 5829 mean_circ: 0.950477
Ran for 139550 frames Cell Events: 5933 mean_circ: 0.950386
Ran for 140050 frames Cell Events: 5955 mean_circ: 0.950383
Ran for 141050 frames Cell Events: 6003 mean_circ: 0.950318
Ran for 141550 frames Cell Events: 6024 mean_circ: 0.950296
Ran for 142050 frames Cell Events: 6041 mean_circ: 0.950298
Ran for 142550 frames Cell Events: 6068 mean_circ: 0.950295
Ran for 143050 frames Cell Events: 6086 mean_circ: 0.950313
Ran for 143550 frames Cell Events: 6102 mean_circ: 0.9503
Ran for 144550 frames Cell Events: 6137 mean_circ: 0.950275
Ran for 145550 frames Cell Events: 6182 mean_circ: 0.950338
Ran for 147050 frames Cell Events: 6268 mean_circ: 0.95037
Ran for 147550 frames Cell Events: 6295 mean_circ: 0.95038
Ran for 148050 frames Cell Events: 6318 mean_circ: 0.950403
Ran for 153050 frames Cell Events: 6546 mean_circ: 0.950407
Ran for 153550 frames Cell Events: 6564 mean_circ: 0.95041
Ran for 154550 frames Cell Events: 6607 mean_circ: 0.950358
Ran for 155550 frames Cell Events: 6661 mean_circ: 0.950383
Ran for 156050 frames Cell Events: 6690 mean_circ: 0.950348
Ran for 157550 frames Cell Events: 6760 mean_circ: 0.950292
Ran for 158050 frames Cell Events: 6779 mean_circ: 0.950294
Ran for 158550 frames Cell Events: 6797 mean_circ: 0.950327
Ran for 159050 frames Cell Events: 6829 mean_circ: 0.950305
Ran for 160050 frames Cell Events: 6866 mean_circ: 0.950308
Ran for 160550 frames Cell Events: 6889 mean_circ: 0.950297
Ran for 161050 frames Cell Events: 6912 mean_circ: 0.950287
Ran for 161550 frames Cell Events: 6941 mean_circ: 0.95029
Ran for 162050 frames Cell Events: 6956 mean_circ: 0.950329
Ran for 162550 frames Cell Events: 6969 mean_circ: 0.950329
Ran for 163050 frames Cell Events: 6988 mean_circ: 0.950334
Ran for 164050 frames Cell Events: 7030 mean_circ: 0.950383
Ran for 164550 frames Cell Events: 7059 mean_circ: 0.950388
Ran for 166050 frames Cell Events: 7121 mean_circ: 0.950391
Ran for 168050 frames Cell Events: 7229 mean_circ: 0.950396
Ran for 169050 frames Cell Events: 7280 mean_circ: 0.950429
Ran for 171050 frames Cell Events: 7368 mean_circ: 0.950442
Ran for 171550 frames Cell Events: 7387 mean_circ: 0.950443
Ran for 174550 frames Cell Events: 7551 mean_circ: 0.950522
Ran for 176550 frames Cell Events: 7645 mean_circ: 0.95048
Ran for 177050 frames Cell Events: 7671 mean_circ: 0.950507
Ran for 179550 frames Cell Events: 7807 mean_circ: 0.950495
Ran for 181050 frames Cell Events: 7885 mean_circ: 0.950564
Ran for 182050 frames Cell Events: 7943 mean_circ: 0.950558
Ran for 183050 frames Cell Events: 7992 mean_circ: 0.950575
Ran for 184050 frames Cell Events: 8045 mean_circ: 0.950601
Ran for 184550 frames Cell Events: 8069 mean_circ: 0.950603
Ran for 185050 frames Cell Events: 8093 mean_circ: 0.950592
Ran for 186550 frames Cell Events: 8176 mean_circ: 0.950552
Ran for 187550 frames Cell Events: 8237 mean_circ: 0.950598
Ran for 188550 frames Cell Events: 8279 mean_circ: 0.950631
Ran for 189550 frames Cell Events: 8335 mean_circ: 0.950637
Ran for 190050 frames Cell Events: 8365 mean_circ: 0.950649
Ran for 191550 frames Cell Events: 8455 mean_circ: 0.95066
Ran for 192050 frames Cell Events: 8485 mean_circ: 0.950682
Ran for 192550 frames Cell Events: 8510 mean_circ: 0.950682
Ran for 195050 frames Cell Events: 8638 mean_circ: 0.950717
Ran for 196050 frames Cell Events: 8696 mean_circ: 0.950741
Ran for 196550 frames Cell Events: 8722 mean_circ: 0.950751
Ran for 197050 frames Cell Events: 8751 mean_circ: 0.950718
Ran for 198050 frames Cell Events: 8806 mean_circ: 0.950713
Ran for 199050 frames Cell Events: 8853 mean_circ: 0.950732
Ran for 200050 frames Cell Events: 8898 mean_circ: 0.950757
Ran for 200550 frames Cell Events: 8931 mean_circ: 0.950779
Ran for 201050 frames Cell Events: 8952 mean_circ: 0.950801
Ran for 204550 frames Cell Events: 9099 mean_circ: 0.950838



End this measurment..



Stopping time: 1.67545e+009
****************************************
Measurement finished

Mean Circularitiy: 0.950829
Mean Area: 606.454
Mean FL-1 intensity: 3606.48
Mean FL-2 intensity: 3817.94
Mean FL-3 intensity: 0

frames processed: 203808
Total Time: 102410ms
Number of Runs (new bg img) 0
Frames collected: 204681
Skipped frames: 104216
Rescued Frames: 2907168
Samples DAQ: 51170854
Samples written: 102340508
Samples per Channel: 51170254
FPS: 1998.64

Samples per second: 499661
Expected samples/s: 500000

Events: 9103
Events per second: 88.8879
jumped back (PMT): 3799
#Frames read, frequency
# 0 frames :316832
# 1 frames :76420
# 2 frames :3716
# 3 frames :1500
# 4 frames :942
# 5 frames :603
# 6 frames :1203
# 7 frames :1435
# 8 frames :1126
# 9 frames :813
# 10 frames :802
# 11 frames :608
# 12 frames :598
# 13 frames :372
# 14 frames :276
# 15 frames :255
# 16 frames :247
# 17 frames :209
# 18 frames :185
# 19 frames :156
# 20 frames :139
# 21 frames :124
# 22 frames :126
# 23 frames :88
# 24 frames :63
# 25 frames :69
# 26 frames :51
# 27 frames :50
# 28 frames :33
# 29 frames :26
# 30 frames :29
# 31 frames :35
# 32 frames :22
# 33 frames :27
# 34 frames :22
# 35 frames :17
# 36 frames :14
# 37 frames :9
# 38 frames :21
# 39 frames :9
# 40 frames :12
# 41 frames :7
# 42 frames :4
# 43 frames :4
# 44 frames :6
# 45 frames :3
# 46 frames :5
# 47 frames :1
# 49 frames :6
# 50 frames :3
# 51 frames :1
# 52 frames :1
# 53 frames :1
# 54 frames :2
# 57 frames :1
# 60 frames :2
# 61 frames :1
# 68 frames :1
# 70 frames :1
# 72 frames :1
# 78 frames :1
# 83 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.4619
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0