Click on the log titles below to expand the log entries present in this DC resource.

dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "d36571f00bb34b7dc35919d441d05c94",
"name": "M1_20170515_1916_dsRNA_D2_col.tdms"
}
],
"libraries": {
"dclab": "0.59.1",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.6:8b6ee5b, Oct 2 2023 14:57:12",
"implementation": "CPython",
"version": "3.11.6"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "13:26:42"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 303:
Not all features have the same length! Limiting output event count to 8282 (max 8283) in '8282'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.3587
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.92306e+007
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 2550 frames Cell Events: 151 mean_circ: 0.94283
Ran for 3050 frames Cell Events: 185 mean_circ: 0.94283
Ran for 5050 frames Cell Events: 296 mean_circ: 0.942861
Ran for 6550 frames Cell Events: 403 mean_circ: 0.941817
Ran for 11050 frames Cell Events: 705 mean_circ: 0.942216
Ran for 13550 frames Cell Events: 856 mean_circ: 0.942855
Ran for 14550 frames Cell Events: 914 mean_circ: 0.942652
Ran for 17050 frames Cell Events: 1028 mean_circ: 0.942496
Ran for 17550 frames Cell Events: 1066 mean_circ: 0.942331
Ran for 18550 frames Cell Events: 1136 mean_circ: 0.942342
Ran for 19050 frames Cell Events: 1167 mean_circ: 0.942291
Ran for 20050 frames Cell Events: 1223 mean_circ: 0.942392
Ran for 20550 frames Cell Events: 1255 mean_circ: 0.942754
Ran for 23050 frames Cell Events: 1420 mean_circ: 0.942976
Ran for 24050 frames Cell Events: 1482 mean_circ: 0.942863
Ran for 24550 frames Cell Events: 1495 mean_circ: 0.942848
Ran for 25050 frames Cell Events: 1516 mean_circ: 0.942545
Ran for 25550 frames Cell Events: 1546 mean_circ: 0.942436
Ran for 27050 frames Cell Events: 1626 mean_circ: 0.942331
Ran for 27550 frames Cell Events: 1652 mean_circ: 0.942579
Ran for 28050 frames Cell Events: 1677 mean_circ: 0.942631
Ran for 28550 frames Cell Events: 1694 mean_circ: 0.942656
Ran for 29050 frames Cell Events: 1719 mean_circ: 0.942863
Ran for 31550 frames Cell Events: 1856 mean_circ: 0.943039
Ran for 34550 frames Cell Events: 2021 mean_circ: 0.942593
Ran for 35050 frames Cell Events: 2039 mean_circ: 0.942599
Ran for 35550 frames Cell Events: 2063 mean_circ: 0.942457
Ran for 36550 frames Cell Events: 2110 mean_circ: 0.942333
Ran for 37050 frames Cell Events: 2142 mean_circ: 0.942523
Ran for 37550 frames Cell Events: 2165 mean_circ: 0.942648
Ran for 38550 frames Cell Events: 2210 mean_circ: 0.942623
Ran for 39050 frames Cell Events: 2228 mean_circ: 0.942514
Ran for 40550 frames Cell Events: 2309 mean_circ: 0.942458
Ran for 41550 frames Cell Events: 2354 mean_circ: 0.942327
Ran for 42050 frames Cell Events: 2391 mean_circ: 0.942327
Ran for 42550 frames Cell Events: 2421 mean_circ: 0.942208
Ran for 43050 frames Cell Events: 2461 mean_circ: 0.942406
Ran for 43550 frames Cell Events: 2497 mean_circ: 0.942317
Ran for 44050 frames Cell Events: 2509 mean_circ: 0.942522
Ran for 46050 frames Cell Events: 2578 mean_circ: 0.942698
Ran for 48550 frames Cell Events: 2691 mean_circ: 0.943037
Ran for 49550 frames Cell Events: 2758 mean_circ: 0.943182
Ran for 51550 frames Cell Events: 2905 mean_circ: 0.942971
Ran for 52050 frames Cell Events: 2924 mean_circ: 0.943002
Ran for 53050 frames Cell Events: 2962 mean_circ: 0.942998
Ran for 54550 frames Cell Events: 3046 mean_circ: 0.94293
Ran for 55050 frames Cell Events: 3067 mean_circ: 0.9429
Ran for 56050 frames Cell Events: 3119 mean_circ: 0.942846
Ran for 56550 frames Cell Events: 3147 mean_circ: 0.942844
Ran for 58050 frames Cell Events: 3236 mean_circ: 0.942821
Ran for 58550 frames Cell Events: 3265 mean_circ: 0.942887
Ran for 59550 frames Cell Events: 3331 mean_circ: 0.942959
Ran for 61050 frames Cell Events: 3447 mean_circ: 0.943017
Ran for 62550 frames Cell Events: 3561 mean_circ: 0.942725
Ran for 63050 frames Cell Events: 3595 mean_circ: 0.942807
Ran for 63550 frames Cell Events: 3633 mean_circ: 0.942885
Ran for 64050 frames Cell Events: 3665 mean_circ: 0.943022
Ran for 64550 frames Cell Events: 3688 mean_circ: 0.943001
Ran for 65550 frames Cell Events: 3739 mean_circ: 0.94297
Ran for 67550 frames Cell Events: 3852 mean_circ: 0.943064
Ran for 70050 frames Cell Events: 4015 mean_circ: 0.943061
Ran for 72050 frames Cell Events: 4143 mean_circ: 0.943174
Ran for 73050 frames Cell Events: 4195 mean_circ: 0.943167
Ran for 73550 frames Cell Events: 4223 mean_circ: 0.943171
Ran for 74050 frames Cell Events: 4239 mean_circ: 0.943202
Ran for 76050 frames Cell Events: 4382 mean_circ: 0.943373
Ran for 76550 frames Cell Events: 4404 mean_circ: 0.943366
Ran for 77050 frames Cell Events: 4416 mean_circ: 0.943397
Ran for 79050 frames Cell Events: 4533 mean_circ: 0.943484
Ran for 79550 frames Cell Events: 4563 mean_circ: 0.943529
Ran for 80050 frames Cell Events: 4583 mean_circ: 0.943536
Ran for 81050 frames Cell Events: 4653 mean_circ: 0.943434
Ran for 82050 frames Cell Events: 4719 mean_circ: 0.943161
Ran for 83050 frames Cell Events: 4773 mean_circ: 0.943095
Ran for 84550 frames Cell Events: 4874 mean_circ: 0.942981
Ran for 85550 frames Cell Events: 4926 mean_circ: 0.943028
Ran for 86550 frames Cell Events: 4980 mean_circ: 0.943093
Ran for 87050 frames Cell Events: 5000 mean_circ: 0.943128
Ran for 87550 frames Cell Events: 5028 mean_circ: 0.943129
Ran for 88050 frames Cell Events: 5050 mean_circ: 0.943111
Ran for 89550 frames Cell Events: 5127 mean_circ: 0.942948
Ran for 90050 frames Cell Events: 5153 mean_circ: 0.942935
Ran for 90550 frames Cell Events: 5165 mean_circ: 0.94292
Ran for 91050 frames Cell Events: 5191 mean_circ: 0.942942
Ran for 92050 frames Cell Events: 5246 mean_circ: 0.942944
Ran for 93050 frames Cell Events: 5309 mean_circ: 0.942897
Ran for 93550 frames Cell Events: 5343 mean_circ: 0.942878
Ran for 94050 frames Cell Events: 5372 mean_circ: 0.942878
Ran for 94550 frames Cell Events: 5398 mean_circ: 0.942902
Ran for 96550 frames Cell Events: 5516 mean_circ: 0.942964
Ran for 98550 frames Cell Events: 5630 mean_circ: 0.942923
Ran for 99050 frames Cell Events: 5658 mean_circ: 0.942916
Ran for 99550 frames Cell Events: 5690 mean_circ: 0.942953
Ran for 100050 frames Cell Events: 5717 mean_circ: 0.942956
Ran for 100550 frames Cell Events: 5752 mean_circ: 0.942956
Ran for 102550 frames Cell Events: 5852 mean_circ: 0.942983
Ran for 103050 frames Cell Events: 5866 mean_circ: 0.942995
Ran for 103550 frames Cell Events: 5882 mean_circ: 0.942999
Ran for 105050 frames Cell Events: 5930 mean_circ: 0.942985
Ran for 105550 frames Cell Events: 5958 mean_circ: 0.943013
Ran for 107050 frames Cell Events: 6069 mean_circ: 0.942869
Ran for 107550 frames Cell Events: 6095 mean_circ: 0.942907
Ran for 108550 frames Cell Events: 6134 mean_circ: 0.942968
Ran for 109050 frames Cell Events: 6169 mean_circ: 0.943044
Ran for 109550 frames Cell Events: 6190 mean_circ: 0.943003
Ran for 110550 frames Cell Events: 6245 mean_circ: 0.942923
Ran for 112550 frames Cell Events: 6359 mean_circ: 0.942981
Ran for 113550 frames Cell Events: 6433 mean_circ: 0.943005
Ran for 114050 frames Cell Events: 6451 mean_circ: 0.942959
Ran for 114550 frames Cell Events: 6471 mean_circ: 0.942967
Ran for 117050 frames Cell Events: 6633 mean_circ: 0.942911
Ran for 118550 frames Cell Events: 6725 mean_circ: 0.942952
Ran for 120550 frames Cell Events: 6852 mean_circ: 0.942943
Ran for 122550 frames Cell Events: 6974 mean_circ: 0.942949
Ran for 123550 frames Cell Events: 7033 mean_circ: 0.942985
Ran for 125550 frames Cell Events: 7172 mean_circ: 0.942806
Ran for 127050 frames Cell Events: 7255 mean_circ: 0.942762
Ran for 127550 frames Cell Events: 7296 mean_circ: 0.942691
Ran for 128050 frames Cell Events: 7321 mean_circ: 0.942688
Ran for 128550 frames Cell Events: 7347 mean_circ: 0.942732
Ran for 131050 frames Cell Events: 7489 mean_circ: 0.942655
Ran for 132050 frames Cell Events: 7580 mean_circ: 0.942639
Ran for 133050 frames Cell Events: 7657 mean_circ: 0.942611
Ran for 134550 frames Cell Events: 7761 mean_circ: 0.942561
Ran for 136050 frames Cell Events: 7843 mean_circ: 0.942516
Ran for 138550 frames Cell Events: 8001 mean_circ: 0.942499
Ran for 139550 frames Cell Events: 8060 mean_circ: 0.942427
Ran for 140550 frames Cell Events: 8118 mean_circ: 0.942371
Ran for 142050 frames Cell Events: 8198 mean_circ: 0.942409
Ran for 143050 frames Cell Events: 8283 mean_circ: 0.942371



End this measurment..



Stopping time: 3.93023e+007
****************************************
Measurement finished

Mean Circularitiy: 0.94237
Mean Area: 686.198
Mean FL-1 intensity: 3684.95
Mean FL-2 intensity: 4956.49
Mean FL-3 intensity: 0

frames processed: 138976
Total Time: 71617.6ms
Number of Runs (new bg img) 0
Frames collected: 143071
Skipped frames: 115865
Rescued Frames: 5172168
Samples DAQ: 35767646
Samples written: 71535196
Samples per Channel: 35767598
FPS: 1997.71

Samples per second: 499424
Expected samples/s: 500000

Events: 8283
Events per second: 115.656
jumped back (PMT): 2694
#Frames read, frequency
# 0 frames :229133
# 1 frames :44699
# 2 frames :2248
# 3 frames :935
# 4 frames :581
# 5 frames :260
# 6 frames :824
# 7 frames :1239
# 8 frames :746
# 9 frames :593
# 10 frames :696
# 11 frames :412
# 12 frames :285
# 13 frames :189
# 14 frames :203
# 15 frames :218
# 16 frames :188
# 17 frames :166
# 18 frames :128
# 19 frames :99
# 20 frames :93
# 21 frames :93
# 22 frames :87
# 23 frames :59
# 24 frames :86
# 25 frames :52
# 26 frames :42
# 27 frames :37
# 28 frames :42
# 29 frames :30
# 30 frames :40
# 31 frames :31
# 32 frames :18
# 33 frames :19
# 34 frames :16
# 35 frames :17
# 36 frames :23
# 37 frames :15
# 38 frames :14
# 39 frames :13
# 40 frames :8
# 41 frames :9
# 42 frames :9
# 43 frames :7
# 44 frames :10
# 45 frames :7
# 46 frames :5
# 47 frames :6
# 48 frames :4
# 49 frames :3
# 50 frames :9
# 51 frames :3
# 52 frames :2
# 53 frames :6
# 54 frames :4
# 56 frames :2
# 57 frames :3
# 58 frames :2
# 59 frames :1
# 60 frames :1
# 62 frames :4
# 63 frames :2
# 65 frames :2
# 66 frames :1
# 69 frames :1
# 70 frames :3
# 72 frames :1
# 73 frames :1
# 75 frames :1
# 76 frames :1
# 77 frames :1
# 78 frames :1
# 79 frames :1
# 80 frames :2
# 85 frames :1
# 93 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.3587
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0