Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.25.25 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "b4264dae58b96327db774a6088459314",
"name": "M1_20170603_1936_dsRNA_D7_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:25:46"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8804 (max 8805) in '8804'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0748
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.68202e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 3550 frames Cell Events: 212 mean_circ: 0.950591
Ran for 5050 frames Cell Events: 303 mean_circ: 0.950456
Ran for 5550 frames Cell Events: 336 mean_circ: 0.951452
Ran for 6550 frames Cell Events: 386 mean_circ: 0.952035
Ran for 10050 frames Cell Events: 619 mean_circ: 0.95149
Ran for 10550 frames Cell Events: 639 mean_circ: 0.951583
Ran for 12550 frames Cell Events: 712 mean_circ: 0.950286
Ran for 13050 frames Cell Events: 733 mean_circ: 0.950595
Ran for 13550 frames Cell Events: 755 mean_circ: 0.950821
Ran for 15550 frames Cell Events: 872 mean_circ: 0.949982
Ran for 16550 frames Cell Events: 917 mean_circ: 0.950113
Ran for 17550 frames Cell Events: 964 mean_circ: 0.950184
Ran for 19050 frames Cell Events: 1056 mean_circ: 0.950119
Ran for 19550 frames Cell Events: 1085 mean_circ: 0.949939
Ran for 20550 frames Cell Events: 1144 mean_circ: 0.950077
Ran for 21050 frames Cell Events: 1163 mean_circ: 0.949995
Ran for 22050 frames Cell Events: 1220 mean_circ: 0.949776
Ran for 22550 frames Cell Events: 1245 mean_circ: 0.950074
Ran for 24050 frames Cell Events: 1307 mean_circ: 0.95027
Ran for 24550 frames Cell Events: 1332 mean_circ: 0.950317
Ran for 25050 frames Cell Events: 1349 mean_circ: 0.950248
Ran for 25550 frames Cell Events: 1369 mean_circ: 0.95037
Ran for 26550 frames Cell Events: 1400 mean_circ: 0.950203
Ran for 27050 frames Cell Events: 1421 mean_circ: 0.950251
Ran for 27550 frames Cell Events: 1447 mean_circ: 0.95043
Ran for 28050 frames Cell Events: 1476 mean_circ: 0.950375
Ran for 28550 frames Cell Events: 1508 mean_circ: 0.950459
Ran for 30050 frames Cell Events: 1609 mean_circ: 0.950574
Ran for 31050 frames Cell Events: 1665 mean_circ: 0.950614
Ran for 31550 frames Cell Events: 1694 mean_circ: 0.95062
Ran for 32050 frames Cell Events: 1723 mean_circ: 0.95065
Ran for 32550 frames Cell Events: 1750 mean_circ: 0.950437
Ran for 33550 frames Cell Events: 1802 mean_circ: 0.950147
Ran for 34550 frames Cell Events: 1881 mean_circ: 0.950278
Ran for 35050 frames Cell Events: 1910 mean_circ: 0.950353
Ran for 36050 frames Cell Events: 1966 mean_circ: 0.950334
Ran for 36550 frames Cell Events: 1991 mean_circ: 0.950203
Ran for 37050 frames Cell Events: 2005 mean_circ: 0.950242
Ran for 39050 frames Cell Events: 2116 mean_circ: 0.950368
Ran for 39550 frames Cell Events: 2146 mean_circ: 0.950362
Ran for 40050 frames Cell Events: 2173 mean_circ: 0.950212
Ran for 41050 frames Cell Events: 2217 mean_circ: 0.950246
Ran for 42550 frames Cell Events: 2313 mean_circ: 0.950573
Ran for 45050 frames Cell Events: 2463 mean_circ: 0.950589
Ran for 45550 frames Cell Events: 2478 mean_circ: 0.950526
Ran for 48050 frames Cell Events: 2605 mean_circ: 0.950402
Ran for 49050 frames Cell Events: 2675 mean_circ: 0.950264
Ran for 49550 frames Cell Events: 2700 mean_circ: 0.950171
Ran for 50550 frames Cell Events: 2747 mean_circ: 0.950159
Ran for 51050 frames Cell Events: 2759 mean_circ: 0.950216
Ran for 56050 frames Cell Events: 3046 mean_circ: 0.949974
Ran for 56550 frames Cell Events: 3058 mean_circ: 0.950005
Ran for 57050 frames Cell Events: 3064 mean_circ: 0.950021
Ran for 57550 frames Cell Events: 3086 mean_circ: 0.950085
Ran for 58050 frames Cell Events: 3115 mean_circ: 0.95016
Ran for 59550 frames Cell Events: 3180 mean_circ: 0.950225
Ran for 60050 frames Cell Events: 3201 mean_circ: 0.950257
Ran for 61050 frames Cell Events: 3261 mean_circ: 0.950151
Ran for 61550 frames Cell Events: 3288 mean_circ: 0.950163
Ran for 63050 frames Cell Events: 3367 mean_circ: 0.950073
Ran for 63550 frames Cell Events: 3401 mean_circ: 0.950032
Ran for 64550 frames Cell Events: 3451 mean_circ: 0.950138
Ran for 65050 frames Cell Events: 3474 mean_circ: 0.950084
Ran for 66550 frames Cell Events: 3545 mean_circ: 0.950078
Ran for 67050 frames Cell Events: 3571 mean_circ: 0.950112
Ran for 67550 frames Cell Events: 3588 mean_circ: 0.950162
Ran for 68050 frames Cell Events: 3631 mean_circ: 0.950187
Ran for 69050 frames Cell Events: 3670 mean_circ: 0.950241
Ran for 70550 frames Cell Events: 3743 mean_circ: 0.950252
Ran for 71050 frames Cell Events: 3767 mean_circ: 0.950312
Ran for 71550 frames Cell Events: 3798 mean_circ: 0.950289
Ran for 73050 frames Cell Events: 3874 mean_circ: 0.95037
Ran for 74050 frames Cell Events: 3939 mean_circ: 0.950349
Ran for 75550 frames Cell Events: 4022 mean_circ: 0.950379
Ran for 76050 frames Cell Events: 4059 mean_circ: 0.950379
Ran for 76550 frames Cell Events: 4083 mean_circ: 0.950426
Ran for 77050 frames Cell Events: 4117 mean_circ: 0.950331
Ran for 77550 frames Cell Events: 4159 mean_circ: 0.950331
Ran for 78050 frames Cell Events: 4177 mean_circ: 0.950272
Ran for 78550 frames Cell Events: 4200 mean_circ: 0.950283
Ran for 80050 frames Cell Events: 4268 mean_circ: 0.95028
Ran for 82050 frames Cell Events: 4376 mean_circ: 0.950235
Ran for 82550 frames Cell Events: 4399 mean_circ: 0.950196
Ran for 83550 frames Cell Events: 4462 mean_circ: 0.950189
Ran for 84550 frames Cell Events: 4500 mean_circ: 0.950211
Ran for 85050 frames Cell Events: 4518 mean_circ: 0.95018
Ran for 85550 frames Cell Events: 4547 mean_circ: 0.950208
Ran for 86050 frames Cell Events: 4571 mean_circ: 0.950221
Ran for 87550 frames Cell Events: 4689 mean_circ: 0.949885
Ran for 88050 frames Cell Events: 4721 mean_circ: 0.949867
Ran for 88550 frames Cell Events: 4758 mean_circ: 0.949777
Ran for 90050 frames Cell Events: 4843 mean_circ: 0.949761
Ran for 90550 frames Cell Events: 4864 mean_circ: 0.949784
Ran for 91050 frames Cell Events: 4893 mean_circ: 0.949803
Ran for 94050 frames Cell Events: 5071 mean_circ: 0.949639
Ran for 95550 frames Cell Events: 5172 mean_circ: 0.949418
Ran for 96550 frames Cell Events: 5247 mean_circ: 0.949391
Ran for 98050 frames Cell Events: 5325 mean_circ: 0.949446
Ran for 98550 frames Cell Events: 5341 mean_circ: 0.949397
Ran for 99050 frames Cell Events: 5368 mean_circ: 0.94942
Ran for 100050 frames Cell Events: 5421 mean_circ: 0.949412
Ran for 100550 frames Cell Events: 5442 mean_circ: 0.949404
Ran for 101550 frames Cell Events: 5511 mean_circ: 0.94943
Ran for 102550 frames Cell Events: 5557 mean_circ: 0.949286
Ran for 103550 frames Cell Events: 5595 mean_circ: 0.9493
Ran for 105050 frames Cell Events: 5681 mean_circ: 0.949332
Ran for 105550 frames Cell Events: 5694 mean_circ: 0.949334
Ran for 107550 frames Cell Events: 5830 mean_circ: 0.949401
Ran for 108050 frames Cell Events: 5858 mean_circ: 0.949436
Ran for 109050 frames Cell Events: 5907 mean_circ: 0.949428
Ran for 110050 frames Cell Events: 5959 mean_circ: 0.949386
Ran for 111550 frames Cell Events: 6013 mean_circ: 0.949348
Ran for 113050 frames Cell Events: 6088 mean_circ: 0.949432
Ran for 113550 frames Cell Events: 6123 mean_circ: 0.949403
Ran for 114550 frames Cell Events: 6172 mean_circ: 0.949409
Ran for 115050 frames Cell Events: 6201 mean_circ: 0.949428
Ran for 115550 frames Cell Events: 6222 mean_circ: 0.949425
Ran for 116050 frames Cell Events: 6243 mean_circ: 0.949425
Ran for 118550 frames Cell Events: 6382 mean_circ: 0.949294
Ran for 122050 frames Cell Events: 6581 mean_circ: 0.94918
Ran for 124050 frames Cell Events: 6698 mean_circ: 0.949183
Ran for 128050 frames Cell Events: 6859 mean_circ: 0.949214
Ran for 128550 frames Cell Events: 6878 mean_circ: 0.949218
Ran for 130050 frames Cell Events: 6964 mean_circ: 0.94912
Ran for 131050 frames Cell Events: 7018 mean_circ: 0.949085
Ran for 132050 frames Cell Events: 7078 mean_circ: 0.949037
Ran for 132550 frames Cell Events: 7109 mean_circ: 0.948995
Ran for 135050 frames Cell Events: 7250 mean_circ: 0.948976
Ran for 137050 frames Cell Events: 7351 mean_circ: 0.948909
Ran for 137550 frames Cell Events: 7378 mean_circ: 0.948878
Ran for 138550 frames Cell Events: 7439 mean_circ: 0.948934
Ran for 140050 frames Cell Events: 7509 mean_circ: 0.9489
Ran for 140550 frames Cell Events: 7525 mean_circ: 0.94892
Ran for 141050 frames Cell Events: 7558 mean_circ: 0.9489
Ran for 141550 frames Cell Events: 7582 mean_circ: 0.948922
Ran for 142550 frames Cell Events: 7629 mean_circ: 0.948953
Ran for 143050 frames Cell Events: 7654 mean_circ: 0.948977
Ran for 143550 frames Cell Events: 7678 mean_circ: 0.948997
Ran for 144550 frames Cell Events: 7729 mean_circ: 0.948963
Ran for 145050 frames Cell Events: 7764 mean_circ: 0.948949
Ran for 146050 frames Cell Events: 7829 mean_circ: 0.948889
Ran for 149050 frames Cell Events: 8045 mean_circ: 0.948947
Ran for 151550 frames Cell Events: 8186 mean_circ: 0.948938
Ran for 153050 frames Cell Events: 8261 mean_circ: 0.948931
Ran for 154050 frames Cell Events: 8313 mean_circ: 0.948829
Ran for 155050 frames Cell Events: 8364 mean_circ: 0.948819
Ran for 155550 frames Cell Events: 8394 mean_circ: 0.948792
Ran for 157050 frames Cell Events: 8464 mean_circ: 0.948822
Ran for 158050 frames Cell Events: 8509 mean_circ: 0.948755
Ran for 160050 frames Cell Events: 8585 mean_circ: 0.948712
Ran for 160550 frames Cell Events: 8597 mean_circ: 0.948702
Ran for 161050 frames Cell Events: 8624 mean_circ: 0.9487
Ran for 162550 frames Cell Events: 8706 mean_circ: 0.94872
Ran for 163550 frames Cell Events: 8761 mean_circ: 0.94867
Ran for 164050 frames Cell Events: 8779 mean_circ: 0.94865



End this measurment..



Stopping time: 1.6821e+009
****************************************
Measurement finished

Mean Circularitiy: 0.948665
Mean Area: 610.242
Mean FL-1 intensity: 3815.29
Mean FL-2 intensity: 3079.26
Mean FL-3 intensity: 0

frames processed: 161980
Total Time: 82324ms
Number of Runs (new bg img) 0
Frames collected: 164509
Skipped frames: 166999
Rescued Frames: 4651303
Samples DAQ: 41128713
Samples written: 82254666
Samples per Channel: 41127333
FPS: 1998.31

Samples per second: 499579
Expected samples/s: 500000

Events: 8805
Events per second: 106.955
jumped back (PMT): 4062
#Frames read, frequency
# 0 frames :248075
# 1 frames :67287
# 2 frames :3436
# 3 frames :1145
# 4 frames :614
# 5 frames :237
# 6 frames :2407
# 7 frames :1378
# 8 frames :1055
# 9 frames :576
# 10 frames :494
# 11 frames :327
# 12 frames :264
# 13 frames :202
# 14 frames :245
# 15 frames :202
# 16 frames :171
# 17 frames :125
# 18 frames :108
# 19 frames :73
# 20 frames :73
# 21 frames :77
# 22 frames :63
# 23 frames :52
# 24 frames :26
# 25 frames :26
# 26 frames :25
# 27 frames :25
# 28 frames :21
# 29 frames :21
# 30 frames :16
# 31 frames :12
# 32 frames :15
# 33 frames :13
# 34 frames :4
# 35 frames :10
# 36 frames :7
# 37 frames :6
# 38 frames :3
# 39 frames :3
# 40 frames :6
# 41 frames :3
# 42 frames :3
# 43 frames :7
# 45 frames :3
# 46 frames :3
# 47 frames :2
# 48 frames :5
# 49 frames :4
# 50 frames :1
# 51 frames :1
# 54 frames :1
# 55 frames :1
# 57 frames :1
# 58 frames :1
# 59 frames :1
# 66 frames :1
# 70 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0748
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0