Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.51.43 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "9def54bcde64864f685166679e99cab7",
"name": "M1_20170604_1837_dsRNA_E3_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:52:05"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8720 (max 8721) in '8720'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.2915
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.76486e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 4 mean_circ: 0.920317
Ran for 550 frames Cell Events: 25 mean_circ: 0.941363
Ran for 1050 frames Cell Events: 49 mean_circ: 0.93825
Ran for 2050 frames Cell Events: 114 mean_circ: 0.940788
Ran for 3050 frames Cell Events: 168 mean_circ: 0.941695
Ran for 3550 frames Cell Events: 197 mean_circ: 0.943139
Ran for 4550 frames Cell Events: 259 mean_circ: 0.94341
Ran for 5550 frames Cell Events: 308 mean_circ: 0.943843
Ran for 7050 frames Cell Events: 398 mean_circ: 0.943251
Ran for 11050 frames Cell Events: 637 mean_circ: 0.942873
Ran for 12050 frames Cell Events: 688 mean_circ: 0.943579
Ran for 13050 frames Cell Events: 741 mean_circ: 0.944291
Ran for 13550 frames Cell Events: 770 mean_circ: 0.944497
Ran for 14050 frames Cell Events: 796 mean_circ: 0.94472
Ran for 14550 frames Cell Events: 822 mean_circ: 0.944769
Ran for 15550 frames Cell Events: 880 mean_circ: 0.94465
Ran for 19550 frames Cell Events: 1158 mean_circ: 0.944424
Ran for 20050 frames Cell Events: 1184 mean_circ: 0.944303
Ran for 20550 frames Cell Events: 1215 mean_circ: 0.94442
Ran for 21550 frames Cell Events: 1266 mean_circ: 0.944661
Ran for 22050 frames Cell Events: 1294 mean_circ: 0.944189
Ran for 22550 frames Cell Events: 1337 mean_circ: 0.944128
Ran for 23050 frames Cell Events: 1359 mean_circ: 0.944425
Ran for 24550 frames Cell Events: 1478 mean_circ: 0.944269
Ran for 25050 frames Cell Events: 1515 mean_circ: 0.943853
Ran for 28050 frames Cell Events: 1717 mean_circ: 0.94387
Ran for 29050 frames Cell Events: 1782 mean_circ: 0.943402
Ran for 30050 frames Cell Events: 1834 mean_circ: 0.943593
Ran for 31050 frames Cell Events: 1893 mean_circ: 0.94383
Ran for 31550 frames Cell Events: 1931 mean_circ: 0.943909
Ran for 33050 frames Cell Events: 2023 mean_circ: 0.943929
Ran for 33550 frames Cell Events: 2048 mean_circ: 0.943912
Ran for 34550 frames Cell Events: 2115 mean_circ: 0.943995
Ran for 35550 frames Cell Events: 2171 mean_circ: 0.944109
Ran for 36050 frames Cell Events: 2217 mean_circ: 0.944238
Ran for 38550 frames Cell Events: 2395 mean_circ: 0.944282
Ran for 39550 frames Cell Events: 2447 mean_circ: 0.944431
Ran for 42050 frames Cell Events: 2621 mean_circ: 0.944399
Ran for 43550 frames Cell Events: 2726 mean_circ: 0.944401
Ran for 46050 frames Cell Events: 2889 mean_circ: 0.944081
Ran for 47550 frames Cell Events: 3000 mean_circ: 0.944011
Ran for 50050 frames Cell Events: 3119 mean_circ: 0.943888
Ran for 50550 frames Cell Events: 3148 mean_circ: 0.943688
Ran for 51050 frames Cell Events: 3186 mean_circ: 0.943708
Ran for 52050 frames Cell Events: 3265 mean_circ: 0.943392
Ran for 52550 frames Cell Events: 3293 mean_circ: 0.943392
Ran for 53550 frames Cell Events: 3357 mean_circ: 0.943358
Ran for 54050 frames Cell Events: 3393 mean_circ: 0.943251
Ran for 54550 frames Cell Events: 3417 mean_circ: 0.943241
Ran for 55550 frames Cell Events: 3475 mean_circ: 0.943202
Ran for 57050 frames Cell Events: 3594 mean_circ: 0.943144
Ran for 58550 frames Cell Events: 3721 mean_circ: 0.943113
Ran for 59050 frames Cell Events: 3762 mean_circ: 0.943061
Ran for 59550 frames Cell Events: 3800 mean_circ: 0.943014
Ran for 61050 frames Cell Events: 3893 mean_circ: 0.942949
Ran for 61550 frames Cell Events: 3919 mean_circ: 0.943029
Ran for 62550 frames Cell Events: 3982 mean_circ: 0.943045
Ran for 63050 frames Cell Events: 4003 mean_circ: 0.943021
Ran for 64550 frames Cell Events: 4104 mean_circ: 0.943063
Ran for 65550 frames Cell Events: 4161 mean_circ: 0.943033
Ran for 67550 frames Cell Events: 4279 mean_circ: 0.943076
Ran for 68050 frames Cell Events: 4312 mean_circ: 0.943047
Ran for 69550 frames Cell Events: 4437 mean_circ: 0.943002
Ran for 70550 frames Cell Events: 4486 mean_circ: 0.942921
Ran for 71550 frames Cell Events: 4538 mean_circ: 0.942983
Ran for 72050 frames Cell Events: 4564 mean_circ: 0.942967
Ran for 73050 frames Cell Events: 4619 mean_circ: 0.942928
Ran for 73550 frames Cell Events: 4637 mean_circ: 0.942935
Ran for 74050 frames Cell Events: 4674 mean_circ: 0.942943
Ran for 75550 frames Cell Events: 4731 mean_circ: 0.942956
Ran for 76550 frames Cell Events: 4776 mean_circ: 0.942878
Ran for 77550 frames Cell Events: 4830 mean_circ: 0.942787
Ran for 79550 frames Cell Events: 4974 mean_circ: 0.942941
Ran for 83050 frames Cell Events: 5189 mean_circ: 0.9429
Ran for 84050 frames Cell Events: 5245 mean_circ: 0.942975
Ran for 84550 frames Cell Events: 5266 mean_circ: 0.942945
Ran for 86050 frames Cell Events: 5362 mean_circ: 0.942981
Ran for 86550 frames Cell Events: 5376 mean_circ: 0.943013
Ran for 87550 frames Cell Events: 5420 mean_circ: 0.943071
Ran for 88050 frames Cell Events: 5451 mean_circ: 0.943113
Ran for 89050 frames Cell Events: 5521 mean_circ: 0.94297
Ran for 90550 frames Cell Events: 5591 mean_circ: 0.94294
Ran for 92050 frames Cell Events: 5679 mean_circ: 0.943075
Ran for 92550 frames Cell Events: 5700 mean_circ: 0.94308
Ran for 93050 frames Cell Events: 5726 mean_circ: 0.943058
Ran for 94050 frames Cell Events: 5782 mean_circ: 0.943036
Ran for 96050 frames Cell Events: 5913 mean_circ: 0.94302
Ran for 96550 frames Cell Events: 5940 mean_circ: 0.943014
Ran for 101550 frames Cell Events: 6238 mean_circ: 0.942982
Ran for 102050 frames Cell Events: 6260 mean_circ: 0.942894
Ran for 104550 frames Cell Events: 6408 mean_circ: 0.942942
Ran for 105050 frames Cell Events: 6425 mean_circ: 0.942943
Ran for 108050 frames Cell Events: 6639 mean_circ: 0.943019
Ran for 108550 frames Cell Events: 6679 mean_circ: 0.943019
Ran for 109050 frames Cell Events: 6717 mean_circ: 0.943058
Ran for 110050 frames Cell Events: 6785 mean_circ: 0.943056
Ran for 110550 frames Cell Events: 6805 mean_circ: 0.943066
Ran for 111550 frames Cell Events: 6877 mean_circ: 0.943058
Ran for 113050 frames Cell Events: 6953 mean_circ: 0.943
Ran for 113550 frames Cell Events: 6979 mean_circ: 0.942968
Ran for 114550 frames Cell Events: 7033 mean_circ: 0.942912
Ran for 115050 frames Cell Events: 7064 mean_circ: 0.942912
Ran for 117050 frames Cell Events: 7200 mean_circ: 0.942961
Ran for 120550 frames Cell Events: 7427 mean_circ: 0.942995
Ran for 121050 frames Cell Events: 7442 mean_circ: 0.943005
Ran for 123050 frames Cell Events: 7578 mean_circ: 0.943015
Ran for 123550 frames Cell Events: 7603 mean_circ: 0.943052
Ran for 128550 frames Cell Events: 7953 mean_circ: 0.94308
Ran for 129050 frames Cell Events: 7975 mean_circ: 0.943072
Ran for 130050 frames Cell Events: 8041 mean_circ: 0.943089
Ran for 130550 frames Cell Events: 8072 mean_circ: 0.943108
Ran for 131050 frames Cell Events: 8106 mean_circ: 0.943108
Ran for 131550 frames Cell Events: 8134 mean_circ: 0.943087
Ran for 132550 frames Cell Events: 8181 mean_circ: 0.943113
Ran for 134050 frames Cell Events: 8284 mean_circ: 0.943096
Ran for 136050 frames Cell Events: 8416 mean_circ: 0.943031
Ran for 137050 frames Cell Events: 8488 mean_circ: 0.943059
Ran for 138550 frames Cell Events: 8588 mean_circ: 0.943064



End this measurment..



Stopping time: 1.76493e+009
****************************************
Measurement finished

Mean Circularitiy: 0.943004
Mean Area: 629.342
Mean FL-1 intensity: 5328.59
Mean FL-2 intensity: 5666.69
Mean FL-3 intensity: 0

frames processed: 138127
Total Time: 70272.1ms
Number of Runs (new bg img) 0
Frames collected: 140432
Skipped frames: 130186
Rescued Frames: 4520591
Samples DAQ: 35109689
Samples written: 70215686
Samples per Channel: 35107843
FPS: 1998.4

Samples per second: 499598
Expected samples/s: 500000

Events: 8721
Events per second: 124.103
jumped back (PMT): 2957
#Frames read, frequency
# 0 frames :227889
# 1 frames :37992
# 2 frames :2443
# 3 frames :1020
# 4 frames :633
# 5 frames :326
# 6 frames :781
# 7 frames :1148
# 8 frames :838
# 9 frames :629
# 10 frames :656
# 11 frames :525
# 12 frames :369
# 13 frames :242
# 14 frames :200
# 15 frames :191
# 16 frames :200
# 17 frames :171
# 18 frames :136
# 19 frames :133
# 20 frames :82
# 21 frames :105
# 22 frames :72
# 23 frames :78
# 24 frames :61
# 25 frames :59
# 26 frames :50
# 27 frames :54
# 28 frames :46
# 29 frames :35
# 30 frames :30
# 31 frames :32
# 32 frames :29
# 33 frames :18
# 34 frames :18
# 35 frames :24
# 36 frames :17
# 37 frames :16
# 38 frames :14
# 39 frames :15
# 40 frames :5
# 41 frames :12
# 42 frames :13
# 43 frames :15
# 44 frames :8
# 45 frames :5
# 46 frames :12
# 47 frames :9
# 48 frames :4
# 49 frames :3
# 50 frames :2
# 52 frames :4
# 54 frames :1
# 55 frames :1
# 56 frames :4
# 57 frames :1
# 59 frames :3
# 60 frames :2
# 61 frames :5
# 62 frames :1
# 65 frames :1
# 67 frames :1
# 69 frames :1
# 70 frames :1
# 71 frames :1
# 73 frames :1
# 86 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.2915
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0