Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.34.11 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "7541c15150a492fd84e9556df2e35a6b",
"name": "M1_20170604_1442_dsRNA_G1_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:34:33"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9003 (max 9004) in '9003'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.7764
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.75076e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 82 mean_circ: 0.947156
Ran for 1550 frames Cell Events: 106 mean_circ: 0.94777
Ran for 2050 frames Cell Events: 132 mean_circ: 0.945876
Ran for 3050 frames Cell Events: 187 mean_circ: 0.946943
Ran for 4050 frames Cell Events: 269 mean_circ: 0.946943
Ran for 5550 frames Cell Events: 402 mean_circ: 0.948672
Ran for 6550 frames Cell Events: 464 mean_circ: 0.948456
Ran for 8050 frames Cell Events: 549 mean_circ: 0.947963
Ran for 10550 frames Cell Events: 730 mean_circ: 0.948517
Ran for 12050 frames Cell Events: 826 mean_circ: 0.948291
Ran for 12550 frames Cell Events: 854 mean_circ: 0.947873
Ran for 14050 frames Cell Events: 956 mean_circ: 0.946751
Ran for 15050 frames Cell Events: 1026 mean_circ: 0.947132
Ran for 15550 frames Cell Events: 1047 mean_circ: 0.947304
Ran for 16050 frames Cell Events: 1074 mean_circ: 0.947386
Ran for 17050 frames Cell Events: 1137 mean_circ: 0.94772
Ran for 17550 frames Cell Events: 1163 mean_circ: 0.948022
Ran for 18050 frames Cell Events: 1181 mean_circ: 0.948165
Ran for 19550 frames Cell Events: 1278 mean_circ: 0.948382
Ran for 21050 frames Cell Events: 1364 mean_circ: 0.948322
Ran for 21550 frames Cell Events: 1394 mean_circ: 0.948338
Ran for 22550 frames Cell Events: 1457 mean_circ: 0.948249
Ran for 23050 frames Cell Events: 1484 mean_circ: 0.948355
Ran for 23550 frames Cell Events: 1520 mean_circ: 0.948345
Ran for 24550 frames Cell Events: 1592 mean_circ: 0.948495
Ran for 26550 frames Cell Events: 1733 mean_circ: 0.948516
Ran for 27050 frames Cell Events: 1759 mean_circ: 0.948323
Ran for 27550 frames Cell Events: 1773 mean_circ: 0.948263
Ran for 28050 frames Cell Events: 1814 mean_circ: 0.948255
Ran for 30550 frames Cell Events: 2000 mean_circ: 0.947941
Ran for 31050 frames Cell Events: 2038 mean_circ: 0.948111
Ran for 31550 frames Cell Events: 2062 mean_circ: 0.948136
Ran for 32050 frames Cell Events: 2092 mean_circ: 0.94817
Ran for 32550 frames Cell Events: 2112 mean_circ: 0.948142
Ran for 34050 frames Cell Events: 2219 mean_circ: 0.948044
Ran for 36050 frames Cell Events: 2373 mean_circ: 0.948201
Ran for 36550 frames Cell Events: 2397 mean_circ: 0.948224
Ran for 37050 frames Cell Events: 2434 mean_circ: 0.948279
Ran for 38550 frames Cell Events: 2536 mean_circ: 0.948444
Ran for 39050 frames Cell Events: 2562 mean_circ: 0.948294
Ran for 40550 frames Cell Events: 2682 mean_circ: 0.948144
Ran for 41050 frames Cell Events: 2716 mean_circ: 0.948148
Ran for 43050 frames Cell Events: 2834 mean_circ: 0.94816
Ran for 44550 frames Cell Events: 2925 mean_circ: 0.948294
Ran for 46050 frames Cell Events: 3036 mean_circ: 0.948143
Ran for 46550 frames Cell Events: 3064 mean_circ: 0.94814
Ran for 47550 frames Cell Events: 3128 mean_circ: 0.948163
Ran for 50050 frames Cell Events: 3276 mean_circ: 0.948227
Ran for 51050 frames Cell Events: 3349 mean_circ: 0.948181
Ran for 55050 frames Cell Events: 3651 mean_circ: 0.947902
Ran for 55550 frames Cell Events: 3678 mean_circ: 0.94778
Ran for 58050 frames Cell Events: 3828 mean_circ: 0.94786
Ran for 59050 frames Cell Events: 3894 mean_circ: 0.947838
Ran for 59550 frames Cell Events: 3917 mean_circ: 0.947841
Ran for 60050 frames Cell Events: 3948 mean_circ: 0.947883
Ran for 62050 frames Cell Events: 4096 mean_circ: 0.947714
Ran for 63050 frames Cell Events: 4153 mean_circ: 0.947721
Ran for 67050 frames Cell Events: 4409 mean_circ: 0.947689
Ran for 67550 frames Cell Events: 4431 mean_circ: 0.947688
Ran for 68550 frames Cell Events: 4499 mean_circ: 0.947669
Ran for 72550 frames Cell Events: 4825 mean_circ: 0.947592
Ran for 73550 frames Cell Events: 4906 mean_circ: 0.947598
Ran for 74050 frames Cell Events: 4940 mean_circ: 0.947665
Ran for 75050 frames Cell Events: 5006 mean_circ: 0.947755
Ran for 76050 frames Cell Events: 5074 mean_circ: 0.947722
Ran for 78050 frames Cell Events: 5222 mean_circ: 0.947784
Ran for 78550 frames Cell Events: 5245 mean_circ: 0.947803
Ran for 81550 frames Cell Events: 5480 mean_circ: 0.947907
Ran for 87050 frames Cell Events: 5887 mean_circ: 0.948007
Ran for 88550 frames Cell Events: 5997 mean_circ: 0.948017
Ran for 90050 frames Cell Events: 6116 mean_circ: 0.94796
Ran for 90550 frames Cell Events: 6138 mean_circ: 0.947939
Ran for 91050 frames Cell Events: 6173 mean_circ: 0.947882
Ran for 92550 frames Cell Events: 6279 mean_circ: 0.947912
Ran for 93050 frames Cell Events: 6323 mean_circ: 0.947895
Ran for 94050 frames Cell Events: 6368 mean_circ: 0.947893
Ran for 95550 frames Cell Events: 6465 mean_circ: 0.94784
Ran for 97050 frames Cell Events: 6547 mean_circ: 0.947842
Ran for 99050 frames Cell Events: 6703 mean_circ: 0.947809
Ran for 99550 frames Cell Events: 6736 mean_circ: 0.947809
Ran for 100550 frames Cell Events: 6789 mean_circ: 0.947884
Ran for 102550 frames Cell Events: 6935 mean_circ: 0.94783
Ran for 103050 frames Cell Events: 6964 mean_circ: 0.947819
Ran for 103550 frames Cell Events: 7001 mean_circ: 0.947805
Ran for 104050 frames Cell Events: 7037 mean_circ: 0.947795
Ran for 105050 frames Cell Events: 7108 mean_circ: 0.947807
Ran for 106050 frames Cell Events: 7181 mean_circ: 0.947814
Ran for 106550 frames Cell Events: 7205 mean_circ: 0.947823
Ran for 107550 frames Cell Events: 7267 mean_circ: 0.947812
Ran for 109550 frames Cell Events: 7400 mean_circ: 0.947825
Ran for 111050 frames Cell Events: 7490 mean_circ: 0.94788
Ran for 112550 frames Cell Events: 7575 mean_circ: 0.947866
Ran for 113550 frames Cell Events: 7644 mean_circ: 0.947866
Ran for 115050 frames Cell Events: 7757 mean_circ: 0.947811
Ran for 115550 frames Cell Events: 7802 mean_circ: 0.94781
Ran for 116050 frames Cell Events: 7834 mean_circ: 0.947838
Ran for 117550 frames Cell Events: 7945 mean_circ: 0.947854
Ran for 122050 frames Cell Events: 8249 mean_circ: 0.947599
Ran for 122550 frames Cell Events: 8275 mean_circ: 0.947607
Ran for 123550 frames Cell Events: 8334 mean_circ: 0.947623
Ran for 125050 frames Cell Events: 8437 mean_circ: 0.947555
Ran for 126050 frames Cell Events: 8513 mean_circ: 0.947558
Ran for 126550 frames Cell Events: 8535 mean_circ: 0.947541
Ran for 129050 frames Cell Events: 8717 mean_circ: 0.947582
Ran for 130550 frames Cell Events: 8856 mean_circ: 0.947582
Ran for 131550 frames Cell Events: 8945 mean_circ: 0.947582



End this measurment..



Stopping time: 1.75082e+009
****************************************
Measurement finished

Mean Circularitiy: 0.947598
Mean Area: 624.339
Mean FL-1 intensity: 3756.82
Mean FL-2 intensity: 4337.86
Mean FL-3 intensity: 0

frames processed: 130662
Total Time: 66329.7ms
Number of Runs (new bg img) 0
Frames collected: 132544
Skipped frames: 26563
Rescued Frames: 554227
Samples DAQ: 33136085
Samples written: 66272052
Samples per Channel: 33136026
FPS: 1998.26

Samples per second: 499566
Expected samples/s: 500000

Events: 9004
Events per second: 135.746
jumped back (PMT): 2813
#Frames read, frequency
# 0 frames :216581
# 1 frames :37618
# 2 frames :2248
# 3 frames :807
# 4 frames :493
# 5 frames :237
# 6 frames :1273
# 7 frames :923
# 8 frames :751
# 9 frames :482
# 10 frames :521
# 11 frames :404
# 12 frames :254
# 13 frames :166
# 14 frames :245
# 15 frames :196
# 16 frames :215
# 17 frames :181
# 18 frames :134
# 19 frames :87
# 20 frames :110
# 21 frames :93
# 22 frames :92
# 23 frames :78
# 24 frames :61
# 25 frames :63
# 26 frames :48
# 27 frames :50
# 28 frames :35
# 29 frames :50
# 30 frames :29
# 31 frames :31
# 32 frames :23
# 33 frames :22
# 34 frames :15
# 35 frames :13
# 36 frames :16
# 37 frames :18
# 38 frames :14
# 39 frames :14
# 40 frames :15
# 41 frames :10
# 42 frames :8
# 43 frames :13
# 44 frames :9
# 45 frames :6
# 46 frames :7
# 47 frames :11
# 48 frames :5
# 49 frames :3
# 50 frames :6
# 51 frames :3
# 52 frames :3
# 53 frames :1
# 54 frames :1
# 55 frames :4
# 56 frames :3
# 57 frames :2
# 58 frames :2
# 59 frames :1
# 60 frames :3
# 61 frames :1
# 62 frames :1
# 64 frames :1
# 65 frames :2
# 70 frames :1
# 72 frames :1
# 74 frames :1
# 76 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.7764
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0