Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_19.56.06 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "23f922735b38f8d8c4931a2df1c99470",
"name": "M1_20170523_1538_dsRNA_G2_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:56:28"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8067 (max 8068) in '8067'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0435
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 7.17328e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 3 mean_circ: 0.93868
Ran for 2050 frames Cell Events: 101 mean_circ: 0.951512
Ran for 4550 frames Cell Events: 247 mean_circ: 0.949927
Ran for 7050 frames Cell Events: 377 mean_circ: 0.949897
Ran for 8550 frames Cell Events: 477 mean_circ: 0.950792
Ran for 9050 frames Cell Events: 497 mean_circ: 0.950789
Ran for 9550 frames Cell Events: 531 mean_circ: 0.950736
Ran for 11050 frames Cell Events: 625 mean_circ: 0.950112
Ran for 12050 frames Cell Events: 685 mean_circ: 0.95009
Ran for 13050 frames Cell Events: 738 mean_circ: 0.948807
Ran for 13550 frames Cell Events: 763 mean_circ: 0.948296
Ran for 14050 frames Cell Events: 785 mean_circ: 0.948539
Ran for 14550 frames Cell Events: 808 mean_circ: 0.948998
Ran for 16050 frames Cell Events: 879 mean_circ: 0.949192
Ran for 19550 frames Cell Events: 1107 mean_circ: 0.950163
Ran for 21050 frames Cell Events: 1176 mean_circ: 0.949498
Ran for 25050 frames Cell Events: 1426 mean_circ: 0.949293
Ran for 25550 frames Cell Events: 1456 mean_circ: 0.949052
Ran for 26050 frames Cell Events: 1492 mean_circ: 0.949157
Ran for 26550 frames Cell Events: 1512 mean_circ: 0.949296
Ran for 28050 frames Cell Events: 1595 mean_circ: 0.948827
Ran for 29550 frames Cell Events: 1692 mean_circ: 0.948905
Ran for 30050 frames Cell Events: 1714 mean_circ: 0.948847
Ran for 32050 frames Cell Events: 1845 mean_circ: 0.949103
Ran for 32550 frames Cell Events: 1886 mean_circ: 0.949103
Ran for 33050 frames Cell Events: 1915 mean_circ: 0.949163
Ran for 33550 frames Cell Events: 1938 mean_circ: 0.949068
Ran for 34050 frames Cell Events: 1965 mean_circ: 0.948983
Ran for 35050 frames Cell Events: 2014 mean_circ: 0.948749
Ran for 36550 frames Cell Events: 2101 mean_circ: 0.948732
Ran for 37050 frames Cell Events: 2131 mean_circ: 0.948737
Ran for 40050 frames Cell Events: 2297 mean_circ: 0.948404
Ran for 40550 frames Cell Events: 2314 mean_circ: 0.94846
Ran for 41550 frames Cell Events: 2361 mean_circ: 0.948392
Ran for 44050 frames Cell Events: 2481 mean_circ: 0.948239
Ran for 45050 frames Cell Events: 2534 mean_circ: 0.948244
Ran for 45550 frames Cell Events: 2564 mean_circ: 0.948303
Ran for 46050 frames Cell Events: 2584 mean_circ: 0.948199
Ran for 47050 frames Cell Events: 2646 mean_circ: 0.948102
Ran for 49050 frames Cell Events: 2750 mean_circ: 0.948019
Ran for 49550 frames Cell Events: 2770 mean_circ: 0.947978
Ran for 50550 frames Cell Events: 2817 mean_circ: 0.947927
Ran for 51050 frames Cell Events: 2839 mean_circ: 0.947891
Ran for 53050 frames Cell Events: 2957 mean_circ: 0.947952
Ran for 54050 frames Cell Events: 3012 mean_circ: 0.948
Ran for 55050 frames Cell Events: 3068 mean_circ: 0.947976
Ran for 55550 frames Cell Events: 3098 mean_circ: 0.947987
Ran for 57050 frames Cell Events: 3195 mean_circ: 0.947958
Ran for 57550 frames Cell Events: 3230 mean_circ: 0.948019
Ran for 58550 frames Cell Events: 3294 mean_circ: 0.948128
Ran for 59050 frames Cell Events: 3315 mean_circ: 0.948049
Ran for 60550 frames Cell Events: 3412 mean_circ: 0.947952
Ran for 61550 frames Cell Events: 3466 mean_circ: 0.947825
Ran for 63050 frames Cell Events: 3553 mean_circ: 0.947996
Ran for 66550 frames Cell Events: 3757 mean_circ: 0.948114
Ran for 67550 frames Cell Events: 3813 mean_circ: 0.947919
Ran for 69050 frames Cell Events: 3882 mean_circ: 0.947959
Ran for 69550 frames Cell Events: 3912 mean_circ: 0.947937
Ran for 73050 frames Cell Events: 4144 mean_circ: 0.948026
Ran for 73550 frames Cell Events: 4158 mean_circ: 0.948045
Ran for 74550 frames Cell Events: 4211 mean_circ: 0.94804
Ran for 75050 frames Cell Events: 4236 mean_circ: 0.947965
Ran for 75550 frames Cell Events: 4259 mean_circ: 0.947985
Ran for 76550 frames Cell Events: 4304 mean_circ: 0.947986
Ran for 78050 frames Cell Events: 4394 mean_circ: 0.947917
Ran for 78550 frames Cell Events: 4426 mean_circ: 0.947918
Ran for 79050 frames Cell Events: 4455 mean_circ: 0.947992
Ran for 79550 frames Cell Events: 4493 mean_circ: 0.948037
Ran for 80550 frames Cell Events: 4537 mean_circ: 0.947958
Ran for 81550 frames Cell Events: 4590 mean_circ: 0.947956
Ran for 82050 frames Cell Events: 4617 mean_circ: 0.947985
Ran for 82550 frames Cell Events: 4653 mean_circ: 0.94796
Ran for 83050 frames Cell Events: 4691 mean_circ: 0.947885
Ran for 83550 frames Cell Events: 4725 mean_circ: 0.94789
Ran for 84550 frames Cell Events: 4788 mean_circ: 0.947915
Ran for 85550 frames Cell Events: 4843 mean_circ: 0.947877
Ran for 86050 frames Cell Events: 4874 mean_circ: 0.947865
Ran for 87050 frames Cell Events: 4925 mean_circ: 0.947905
Ran for 87550 frames Cell Events: 4951 mean_circ: 0.947855
Ran for 88050 frames Cell Events: 4977 mean_circ: 0.947902
Ran for 89550 frames Cell Events: 5068 mean_circ: 0.948041
Ran for 90550 frames Cell Events: 5126 mean_circ: 0.948113
Ran for 92050 frames Cell Events: 5193 mean_circ: 0.948177
Ran for 93050 frames Cell Events: 5263 mean_circ: 0.94822
Ran for 93550 frames Cell Events: 5292 mean_circ: 0.948206
Ran for 94550 frames Cell Events: 5334 mean_circ: 0.948153
Ran for 95050 frames Cell Events: 5360 mean_circ: 0.948177
Ran for 95550 frames Cell Events: 5382 mean_circ: 0.948169
Ran for 96550 frames Cell Events: 5432 mean_circ: 0.948166
Ran for 98050 frames Cell Events: 5528 mean_circ: 0.948257
Ran for 99050 frames Cell Events: 5588 mean_circ: 0.948168
Ran for 100050 frames Cell Events: 5648 mean_circ: 0.948169
Ran for 100550 frames Cell Events: 5679 mean_circ: 0.948211
Ran for 101050 frames Cell Events: 5703 mean_circ: 0.948258
Ran for 101550 frames Cell Events: 5733 mean_circ: 0.948264
Ran for 104050 frames Cell Events: 5871 mean_circ: 0.948341
Ran for 105050 frames Cell Events: 5922 mean_circ: 0.94834
Ran for 106550 frames Cell Events: 6009 mean_circ: 0.948287
Ran for 107550 frames Cell Events: 6067 mean_circ: 0.948323
Ran for 108050 frames Cell Events: 6097 mean_circ: 0.948309
Ran for 108550 frames Cell Events: 6131 mean_circ: 0.948325
Ran for 109050 frames Cell Events: 6144 mean_circ: 0.948349
Ran for 111050 frames Cell Events: 6277 mean_circ: 0.948519
Ran for 111550 frames Cell Events: 6299 mean_circ: 0.948486
Ran for 112550 frames Cell Events: 6364 mean_circ: 0.948428
Ran for 114550 frames Cell Events: 6485 mean_circ: 0.948535
Ran for 115050 frames Cell Events: 6506 mean_circ: 0.948545
Ran for 115550 frames Cell Events: 6535 mean_circ: 0.948488
Ran for 117050 frames Cell Events: 6620 mean_circ: 0.948493
Ran for 118050 frames Cell Events: 6672 mean_circ: 0.948542
Ran for 120050 frames Cell Events: 6804 mean_circ: 0.94851
Ran for 120550 frames Cell Events: 6826 mean_circ: 0.948513
Ran for 121050 frames Cell Events: 6859 mean_circ: 0.948506
Ran for 123550 frames Cell Events: 6998 mean_circ: 0.948363
Ran for 124550 frames Cell Events: 7066 mean_circ: 0.948383
Ran for 126050 frames Cell Events: 7146 mean_circ: 0.948392
Ran for 127550 frames Cell Events: 7225 mean_circ: 0.948397
Ran for 129050 frames Cell Events: 7337 mean_circ: 0.948476
Ran for 130050 frames Cell Events: 7405 mean_circ: 0.94844
Ran for 132050 frames Cell Events: 7514 mean_circ: 0.948332
Ran for 135050 frames Cell Events: 7683 mean_circ: 0.948287
Ran for 135550 frames Cell Events: 7706 mean_circ: 0.948323
Ran for 138550 frames Cell Events: 7861 mean_circ: 0.948299
Ran for 139050 frames Cell Events: 7885 mean_circ: 0.948338
Ran for 141050 frames Cell Events: 8020 mean_circ: 0.948337
Ran for 141550 frames Cell Events: 8055 mean_circ: 0.948293



End this measurment..



Stopping time: 7.17399e+008
****************************************
Measurement finished

Mean Circularitiy: 0.948303
Mean Area: 599.24
Mean FL-1 intensity: 2049.87
Mean FL-2 intensity: 2628.34
Mean FL-3 intensity: 0

frames processed: 139132
Total Time: 70939.8ms
Number of Runs (new bg img) 0
Frames collected: 141756
Skipped frames: 53462
Rescued Frames: 1951930
Samples DAQ: 35440596
Samples written: 70878068
Samples per Channel: 35439034
FPS: 1998.26

Samples per second: 499565
Expected samples/s: 500000

Events: 8068
Events per second: 113.73
jumped back (PMT): 2814
#Frames read, frequency
# 0 frames :226503
# 1 frames :45728
# 2 frames :2480
# 3 frames :926
# 4 frames :571
# 5 frames :322
# 6 frames :1075
# 7 frames :989
# 8 frames :731
# 9 frames :558
# 10 frames :612
# 11 frames :415
# 12 frames :298
# 13 frames :192
# 14 frames :213
# 15 frames :224
# 16 frames :194
# 17 frames :177
# 18 frames :141
# 19 frames :120
# 20 frames :94
# 21 frames :100
# 22 frames :86
# 23 frames :71
# 24 frames :60
# 25 frames :60
# 26 frames :42
# 27 frames :36
# 28 frames :43
# 29 frames :23
# 30 frames :26
# 31 frames :31
# 32 frames :26
# 33 frames :25
# 34 frames :22
# 35 frames :16
# 36 frames :13
# 37 frames :15
# 38 frames :10
# 39 frames :9
# 40 frames :13
# 41 frames :8
# 42 frames :6
# 43 frames :4
# 44 frames :4
# 45 frames :8
# 46 frames :8
# 47 frames :4
# 48 frames :1
# 49 frames :3
# 50 frames :2
# 51 frames :2
# 52 frames :4
# 53 frames :6
# 54 frames :4
# 55 frames :1
# 56 frames :2
# 57 frames :3
# 58 frames :2
# 59 frames :2
# 60 frames :2
# 62 frames :3
# 63 frames :1
# 64 frames :1
# 65 frames :1
# 67 frames :1
# 68 frames :1
# 70 frames :1
# 72 frames :1
# 75 frames :1
# 77 frames :1
# 79 frames :1
# 86 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0435
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0