Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_20.08.39 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "ced16d7031613cd0437015809ef56aa5",
"name": "M1_20170523_1849_dsRNA_H2_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "18:09:04"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 10002 (max 10003) in
'10002'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1994
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 7.28801e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 29 mean_circ: 0.96057
Ran for 1550 frames Cell Events: 97 mean_circ: 0.957421
Ran for 2550 frames Cell Events: 185 mean_circ: 0.955402
Ran for 3050 frames Cell Events: 239 mean_circ: 0.955402
Ran for 4050 frames Cell Events: 304 mean_circ: 0.955417
Ran for 4550 frames Cell Events: 336 mean_circ: 0.95365
Ran for 5050 frames Cell Events: 386 mean_circ: 0.95365
Ran for 5550 frames Cell Events: 410 mean_circ: 0.953367
Ran for 6050 frames Cell Events: 434 mean_circ: 0.953687
Ran for 12050 frames Cell Events: 864 mean_circ: 0.953211
Ran for 13550 frames Cell Events: 963 mean_circ: 0.953711
Ran for 14050 frames Cell Events: 995 mean_circ: 0.953591
Ran for 14550 frames Cell Events: 1017 mean_circ: 0.953706
Ran for 16050 frames Cell Events: 1111 mean_circ: 0.953664
Ran for 16550 frames Cell Events: 1146 mean_circ: 0.953954
Ran for 18550 frames Cell Events: 1253 mean_circ: 0.954203
Ran for 20050 frames Cell Events: 1319 mean_circ: 0.95441
Ran for 21050 frames Cell Events: 1389 mean_circ: 0.954062
Ran for 22050 frames Cell Events: 1474 mean_circ: 0.954062
Ran for 24050 frames Cell Events: 1654 mean_circ: 0.95346
Ran for 25050 frames Cell Events: 1717 mean_circ: 0.953216
Ran for 26550 frames Cell Events: 1827 mean_circ: 0.953214
Ran for 27550 frames Cell Events: 1900 mean_circ: 0.953323
Ran for 28550 frames Cell Events: 1981 mean_circ: 0.953323
Ran for 30050 frames Cell Events: 2082 mean_circ: 0.953313
Ran for 30550 frames Cell Events: 2104 mean_circ: 0.953409
Ran for 35050 frames Cell Events: 2447 mean_circ: 0.953489
Ran for 35550 frames Cell Events: 2478 mean_circ: 0.953556
Ran for 37550 frames Cell Events: 2634 mean_circ: 0.953555
Ran for 38050 frames Cell Events: 2671 mean_circ: 0.953535
Ran for 38550 frames Cell Events: 2708 mean_circ: 0.953535
Ran for 39550 frames Cell Events: 2788 mean_circ: 0.953426
Ran for 40050 frames Cell Events: 2821 mean_circ: 0.953384
Ran for 40550 frames Cell Events: 2870 mean_circ: 0.953284
Ran for 41050 frames Cell Events: 2913 mean_circ: 0.953255
Ran for 44050 frames Cell Events: 3154 mean_circ: 0.953313
Ran for 45050 frames Cell Events: 3221 mean_circ: 0.953365
Ran for 45550 frames Cell Events: 3257 mean_circ: 0.953259
Ran for 46550 frames Cell Events: 3341 mean_circ: 0.953214
Ran for 48550 frames Cell Events: 3444 mean_circ: 0.953118
Ran for 49050 frames Cell Events: 3478 mean_circ: 0.953116
Ran for 50050 frames Cell Events: 3550 mean_circ: 0.952972
Ran for 52050 frames Cell Events: 3699 mean_circ: 0.953128
Ran for 52550 frames Cell Events: 3731 mean_circ: 0.953087
Ran for 53050 frames Cell Events: 3767 mean_circ: 0.952955
Ran for 54550 frames Cell Events: 3868 mean_circ: 0.953022
Ran for 57050 frames Cell Events: 4109 mean_circ: 0.952877
Ran for 58050 frames Cell Events: 4191 mean_circ: 0.952801
Ran for 59050 frames Cell Events: 4252 mean_circ: 0.952713
Ran for 60050 frames Cell Events: 4323 mean_circ: 0.952716
Ran for 60550 frames Cell Events: 4364 mean_circ: 0.952718
Ran for 61550 frames Cell Events: 4453 mean_circ: 0.952685
Ran for 62550 frames Cell Events: 4506 mean_circ: 0.952731
Ran for 63050 frames Cell Events: 4544 mean_circ: 0.952756
Ran for 65550 frames Cell Events: 4727 mean_circ: 0.952823
Ran for 66050 frames Cell Events: 4738 mean_circ: 0.952853
Ran for 66550 frames Cell Events: 4761 mean_circ: 0.952861
Ran for 67550 frames Cell Events: 4828 mean_circ: 0.952971
Ran for 69050 frames Cell Events: 4917 mean_circ: 0.953008
Ran for 70050 frames Cell Events: 4995 mean_circ: 0.953037
Ran for 70550 frames Cell Events: 5034 mean_circ: 0.953087
Ran for 73550 frames Cell Events: 5300 mean_circ: 0.953067
Ran for 74050 frames Cell Events: 5316 mean_circ: 0.953091
Ran for 75550 frames Cell Events: 5456 mean_circ: 0.952897
Ran for 76550 frames Cell Events: 5545 mean_circ: 0.952734
Ran for 78550 frames Cell Events: 5698 mean_circ: 0.952738
Ran for 79050 frames Cell Events: 5738 mean_circ: 0.952738
Ran for 79550 frames Cell Events: 5770 mean_circ: 0.952665
Ran for 84050 frames Cell Events: 6148 mean_circ: 0.952851
Ran for 84550 frames Cell Events: 6181 mean_circ: 0.952902
Ran for 86050 frames Cell Events: 6291 mean_circ: 0.952928
Ran for 87050 frames Cell Events: 6351 mean_circ: 0.952959
Ran for 87550 frames Cell Events: 6379 mean_circ: 0.953012
Ran for 88050 frames Cell Events: 6414 mean_circ: 0.953026
Ran for 89550 frames Cell Events: 6499 mean_circ: 0.953069
Ran for 90550 frames Cell Events: 6559 mean_circ: 0.953045
Ran for 91550 frames Cell Events: 6627 mean_circ: 0.95303
Ran for 92050 frames Cell Events: 6657 mean_circ: 0.953066
Ran for 93050 frames Cell Events: 6745 mean_circ: 0.952959
Ran for 93550 frames Cell Events: 6780 mean_circ: 0.95288
Ran for 94550 frames Cell Events: 6853 mean_circ: 0.952937
Ran for 95050 frames Cell Events: 6891 mean_circ: 0.952887
Ran for 95550 frames Cell Events: 6920 mean_circ: 0.952946
Ran for 96050 frames Cell Events: 6952 mean_circ: 0.952921
Ran for 98050 frames Cell Events: 7108 mean_circ: 0.953025
Ran for 98550 frames Cell Events: 7150 mean_circ: 0.953025
Ran for 99050 frames Cell Events: 7182 mean_circ: 0.952978
Ran for 100050 frames Cell Events: 7236 mean_circ: 0.952954
Ran for 100550 frames Cell Events: 7260 mean_circ: 0.952927
Ran for 101550 frames Cell Events: 7328 mean_circ: 0.952897
Ran for 102050 frames Cell Events: 7373 mean_circ: 0.952949
Ran for 102550 frames Cell Events: 7418 mean_circ: 0.952896
Ran for 103050 frames Cell Events: 7445 mean_circ: 0.952963
Ran for 108050 frames Cell Events: 7903 mean_circ: 0.952899
Ran for 108550 frames Cell Events: 7936 mean_circ: 0.952849
Ran for 110550 frames Cell Events: 8077 mean_circ: 0.952887
Ran for 112050 frames Cell Events: 8198 mean_circ: 0.952808
Ran for 112550 frames Cell Events: 8230 mean_circ: 0.952794
Ran for 114550 frames Cell Events: 8361 mean_circ: 0.952775
Ran for 116550 frames Cell Events: 8502 mean_circ: 0.952817
Ran for 117050 frames Cell Events: 8534 mean_circ: 0.952747
Ran for 117550 frames Cell Events: 8561 mean_circ: 0.952747
Ran for 121550 frames Cell Events: 8861 mean_circ: 0.952737
Ran for 123050 frames Cell Events: 8995 mean_circ: 0.952839
Ran for 123550 frames Cell Events: 9035 mean_circ: 0.952794
Ran for 125050 frames Cell Events: 9197 mean_circ: 0.952732
Ran for 125550 frames Cell Events: 9237 mean_circ: 0.95273
Ran for 126550 frames Cell Events: 9293 mean_circ: 0.952739
Ran for 129050 frames Cell Events: 9471 mean_circ: 0.952637
Ran for 130050 frames Cell Events: 9569 mean_circ: 0.952686
Ran for 132050 frames Cell Events: 9728 mean_circ: 0.952771
Ran for 134550 frames Cell Events: 9944 mean_circ: 0.952656



End this measurment.
because stop condition (time/nr of cells) was met

Stopping time: 7.28869e+008
****************************************
Measurement finished

Mean Circularitiy: 0.952686
Mean Area: 594.515
Mean FL-1 intensity: 3471.56
Mean FL-2 intensity: 4288.82
Mean FL-3 intensity: 0

frames processed: 129307
Total Time: 67795.1ms
Number of Runs (new bg img) 0
Frames collected: 135436
Skipped frames: 127601
Rescued Frames: 4184291
Samples DAQ: 33864346
Samples written: 67717660
Samples per Channel: 33858830
FPS: 1997.73

Samples per second: 499429
Expected samples/s: 500000

Events: 10003
Events per second: 147.548
jumped back (PMT): 2593
#Frames read, frequency
# 0 frames :224577
# 1 frames :34700
# 2 frames :1915
# 3 frames :765
# 4 frames :392
# 5 frames :216
# 6 frames :1430
# 7 frames :865
# 8 frames :751
# 9 frames :467
# 10 frames :411
# 11 frames :269
# 12 frames :265
# 13 frames :225
# 14 frames :253
# 15 frames :205
# 16 frames :188
# 17 frames :131
# 18 frames :127
# 19 frames :84
# 20 frames :104
# 21 frames :111
# 22 frames :93
# 23 frames :85
# 24 frames :80
# 25 frames :64
# 26 frames :52
# 27 frames :50
# 28 frames :42
# 29 frames :37
# 30 frames :41
# 31 frames :36
# 32 frames :27
# 33 frames :35
# 34 frames :34
# 35 frames :22
# 36 frames :23
# 37 frames :16
# 38 frames :21
# 39 frames :15
# 40 frames :17
# 41 frames :10
# 42 frames :23
# 43 frames :17
# 44 frames :9
# 45 frames :15
# 46 frames :12
# 47 frames :12
# 48 frames :5
# 49 frames :11
# 50 frames :7
# 51 frames :7
# 52 frames :5
# 53 frames :8
# 54 frames :8
# 55 frames :3
# 56 frames :5
# 57 frames :2
# 58 frames :7
# 59 frames :1
# 60 frames :4
# 61 frames :8
# 62 frames :1
# 63 frames :3
# 64 frames :3
# 66 frames :1
# 67 frames :3
# 68 frames :3
# 69 frames :3
# 70 frames :2
# 72 frames :1
# 74 frames :1
# 76 frames :1
# 79 frames :1
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# 86 frames :3
# 88 frames :1
# 90 frames :1
# 92 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1994
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0