Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_20.06.15 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "e95afb3defad8b0a7cdce516093fb4d2",
"name": "M1_20170523_1808_dsRNA_H3_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "18:06:40"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9781 (max 9782) in '9781'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1896
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 7.26338e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 2050 frames Cell Events: 126 mean_circ: 0.930694
Ran for 4050 frames Cell Events: 209 mean_circ: 0.93104
Ran for 5050 frames Cell Events: 269 mean_circ: 0.931283
Ran for 5550 frames Cell Events: 314 mean_circ: 0.931979
Ran for 6550 frames Cell Events: 377 mean_circ: 0.93274
Ran for 9550 frames Cell Events: 562 mean_circ: 0.935732
Ran for 12550 frames Cell Events: 732 mean_circ: 0.934345
Ran for 15550 frames Cell Events: 974 mean_circ: 0.933971
Ran for 20050 frames Cell Events: 1290 mean_circ: 0.935507
Ran for 21550 frames Cell Events: 1379 mean_circ: 0.935798
Ran for 22050 frames Cell Events: 1408 mean_circ: 0.935641
Ran for 23050 frames Cell Events: 1446 mean_circ: 0.935641
Ran for 23550 frames Cell Events: 1480 mean_circ: 0.935661
Ran for 24550 frames Cell Events: 1527 mean_circ: 0.935748
Ran for 25050 frames Cell Events: 1554 mean_circ: 0.935375
Ran for 26050 frames Cell Events: 1617 mean_circ: 0.935585
Ran for 26550 frames Cell Events: 1653 mean_circ: 0.935547
Ran for 27050 frames Cell Events: 1666 mean_circ: 0.935597
Ran for 28550 frames Cell Events: 1767 mean_circ: 0.93616
Ran for 30050 frames Cell Events: 1853 mean_circ: 0.936258
Ran for 30550 frames Cell Events: 1886 mean_circ: 0.936228
Ran for 31550 frames Cell Events: 1928 mean_circ: 0.93634
Ran for 33550 frames Cell Events: 2044 mean_circ: 0.936476
Ran for 34050 frames Cell Events: 2077 mean_circ: 0.936201
Ran for 34550 frames Cell Events: 2122 mean_circ: 0.936046
Ran for 35050 frames Cell Events: 2155 mean_circ: 0.935938
Ran for 35550 frames Cell Events: 2173 mean_circ: 0.935958
Ran for 38550 frames Cell Events: 2373 mean_circ: 0.935799
Ran for 39550 frames Cell Events: 2448 mean_circ: 0.935626
Ran for 40550 frames Cell Events: 2500 mean_circ: 0.935557
Ran for 41550 frames Cell Events: 2538 mean_circ: 0.935538
Ran for 42050 frames Cell Events: 2576 mean_circ: 0.935587
Ran for 43050 frames Cell Events: 2634 mean_circ: 0.935805
Ran for 43550 frames Cell Events: 2658 mean_circ: 0.935871
Ran for 44050 frames Cell Events: 2684 mean_circ: 0.935759
Ran for 45050 frames Cell Events: 2775 mean_circ: 0.935946
Ran for 46050 frames Cell Events: 2840 mean_circ: 0.936123
Ran for 47050 frames Cell Events: 2907 mean_circ: 0.936126
Ran for 47550 frames Cell Events: 2941 mean_circ: 0.936193
Ran for 48550 frames Cell Events: 3005 mean_circ: 0.936123
Ran for 50050 frames Cell Events: 3110 mean_circ: 0.936087
Ran for 50550 frames Cell Events: 3133 mean_circ: 0.936082
Ran for 52050 frames Cell Events: 3210 mean_circ: 0.935972
Ran for 52550 frames Cell Events: 3244 mean_circ: 0.935994
Ran for 53550 frames Cell Events: 3302 mean_circ: 0.935911
Ran for 55050 frames Cell Events: 3382 mean_circ: 0.936002
Ran for 57050 frames Cell Events: 3507 mean_circ: 0.935796
Ran for 57550 frames Cell Events: 3529 mean_circ: 0.935764
Ran for 58050 frames Cell Events: 3542 mean_circ: 0.935756
Ran for 58550 frames Cell Events: 3564 mean_circ: 0.935781
Ran for 59550 frames Cell Events: 3622 mean_circ: 0.935693
Ran for 60550 frames Cell Events: 3689 mean_circ: 0.935883
Ran for 61050 frames Cell Events: 3724 mean_circ: 0.935824
Ran for 62050 frames Cell Events: 3771 mean_circ: 0.935825
Ran for 63050 frames Cell Events: 3839 mean_circ: 0.935899
Ran for 64550 frames Cell Events: 3921 mean_circ: 0.935843
Ran for 65050 frames Cell Events: 3950 mean_circ: 0.935853
Ran for 67050 frames Cell Events: 4056 mean_circ: 0.935926
Ran for 67550 frames Cell Events: 4078 mean_circ: 0.935911
Ran for 69050 frames Cell Events: 4194 mean_circ: 0.935947
Ran for 70050 frames Cell Events: 4268 mean_circ: 0.936003
Ran for 71050 frames Cell Events: 4322 mean_circ: 0.936015
Ran for 71550 frames Cell Events: 4343 mean_circ: 0.93602
Ran for 74550 frames Cell Events: 4541 mean_circ: 0.936064
Ran for 77550 frames Cell Events: 4767 mean_circ: 0.935823
Ran for 78050 frames Cell Events: 4789 mean_circ: 0.935698
Ran for 78550 frames Cell Events: 4815 mean_circ: 0.935725
Ran for 79050 frames Cell Events: 4859 mean_circ: 0.935806
Ran for 80050 frames Cell Events: 4929 mean_circ: 0.935692
Ran for 81050 frames Cell Events: 5000 mean_circ: 0.935701
Ran for 82050 frames Cell Events: 5069 mean_circ: 0.935752
Ran for 83050 frames Cell Events: 5134 mean_circ: 0.935654
Ran for 85050 frames Cell Events: 5221 mean_circ: 0.935689
Ran for 85550 frames Cell Events: 5252 mean_circ: 0.93573
Ran for 86050 frames Cell Events: 5274 mean_circ: 0.935743
Ran for 86550 frames Cell Events: 5302 mean_circ: 0.935752
Ran for 87050 frames Cell Events: 5338 mean_circ: 0.935472
Ran for 87550 frames Cell Events: 5357 mean_circ: 0.93548
Ran for 88050 frames Cell Events: 5374 mean_circ: 0.935484
Ran for 89550 frames Cell Events: 5475 mean_circ: 0.935366
Ran for 90550 frames Cell Events: 5535 mean_circ: 0.935412
Ran for 91550 frames Cell Events: 5617 mean_circ: 0.935443
Ran for 92050 frames Cell Events: 5649 mean_circ: 0.935475
Ran for 92550 frames Cell Events: 5686 mean_circ: 0.935501
Ran for 93050 frames Cell Events: 5730 mean_circ: 0.935466
Ran for 93550 frames Cell Events: 5754 mean_circ: 0.935497
Ran for 94550 frames Cell Events: 5826 mean_circ: 0.935534
Ran for 95550 frames Cell Events: 5906 mean_circ: 0.935573
Ran for 96550 frames Cell Events: 5957 mean_circ: 0.935733
Ran for 97050 frames Cell Events: 5984 mean_circ: 0.935727
Ran for 99550 frames Cell Events: 6150 mean_circ: 0.93573
Ran for 100550 frames Cell Events: 6224 mean_circ: 0.93573
Ran for 103550 frames Cell Events: 6432 mean_circ: 0.935581
Ran for 104550 frames Cell Events: 6501 mean_circ: 0.93556
Ran for 105050 frames Cell Events: 6542 mean_circ: 0.935551
Ran for 106050 frames Cell Events: 6624 mean_circ: 0.93559
Ran for 106550 frames Cell Events: 6666 mean_circ: 0.935589
Ran for 107050 frames Cell Events: 6698 mean_circ: 0.935636
Ran for 107550 frames Cell Events: 6731 mean_circ: 0.935573
Ran for 108050 frames Cell Events: 6745 mean_circ: 0.935595
Ran for 108550 frames Cell Events: 6772 mean_circ: 0.935595
Ran for 109550 frames Cell Events: 6840 mean_circ: 0.935577
Ran for 111550 frames Cell Events: 6949 mean_circ: 0.935606
Ran for 112550 frames Cell Events: 7022 mean_circ: 0.935598
Ran for 115550 frames Cell Events: 7255 mean_circ: 0.935418
Ran for 116050 frames Cell Events: 7278 mean_circ: 0.935449
Ran for 117550 frames Cell Events: 7369 mean_circ: 0.935372
Ran for 118550 frames Cell Events: 7430 mean_circ: 0.93532
Ran for 119050 frames Cell Events: 7462 mean_circ: 0.935233
Ran for 119550 frames Cell Events: 7515 mean_circ: 0.935233
Ran for 122050 frames Cell Events: 7677 mean_circ: 0.935176
Ran for 123050 frames Cell Events: 7737 mean_circ: 0.935163
Ran for 123550 frames Cell Events: 7771 mean_circ: 0.935192
Ran for 124550 frames Cell Events: 7822 mean_circ: 0.935229
Ran for 125550 frames Cell Events: 7871 mean_circ: 0.935282
Ran for 126050 frames Cell Events: 7895 mean_circ: 0.935316
Ran for 128550 frames Cell Events: 8060 mean_circ: 0.935211
Ran for 129550 frames Cell Events: 8125 mean_circ: 0.935306
Ran for 130050 frames Cell Events: 8150 mean_circ: 0.935352
Ran for 131050 frames Cell Events: 8212 mean_circ: 0.935321
Ran for 131550 frames Cell Events: 8252 mean_circ: 0.93529
Ran for 132550 frames Cell Events: 8305 mean_circ: 0.935291
Ran for 134050 frames Cell Events: 8409 mean_circ: 0.935228
Ran for 134550 frames Cell Events: 8438 mean_circ: 0.935209
Ran for 136050 frames Cell Events: 8539 mean_circ: 0.935279
Ran for 136550 frames Cell Events: 8575 mean_circ: 0.935285
Ran for 137550 frames Cell Events: 8660 mean_circ: 0.935233
Ran for 139050 frames Cell Events: 8752 mean_circ: 0.935291
Ran for 139550 frames Cell Events: 8773 mean_circ: 0.935306
Ran for 140550 frames Cell Events: 8845 mean_circ: 0.935321
Ran for 142050 frames Cell Events: 8938 mean_circ: 0.935232
Ran for 142550 frames Cell Events: 8967 mean_circ: 0.935228
Ran for 144050 frames Cell Events: 9079 mean_circ: 0.935277
Ran for 144550 frames Cell Events: 9102 mean_circ: 0.935359
Ran for 145550 frames Cell Events: 9144 mean_circ: 0.935361
Ran for 148050 frames Cell Events: 9332 mean_circ: 0.93538
Ran for 149050 frames Cell Events: 9390 mean_circ: 0.935423
Ran for 151550 frames Cell Events: 9522 mean_circ: 0.935415
Ran for 153550 frames Cell Events: 9658 mean_circ: 0.935453
Ran for 154050 frames Cell Events: 9683 mean_circ: 0.935464



End this measurment..



Stopping time: 7.26416e+008
****************************************
Measurement finished

Mean Circularitiy: 0.935391
Mean Area: 606.477
Mean FL-1 intensity: 3736.1
Mean FL-2 intensity: 5267.49
Mean FL-3 intensity: 0

frames processed: 151635
Total Time: 77857.1ms
Number of Runs (new bg img) 0
Frames collected: 155545
Skipped frames: 107105
Rescued Frames: 3708716
Samples DAQ: 38886156
Samples written: 77772220
Samples per Channel: 38886110
FPS: 1997.83

Samples per second: 499455
Expected samples/s: 500000

Events: 9782
Events per second: 125.641
jumped back (PMT): 25
#Frames read, frequency
# 0 frames :243706
# 1 frames :54074
# 2 frames :2990
# 3 frames :954
# 4 frames :519
# 5 frames :219
# 6 frames :2310
# 7 frames :1264
# 8 frames :1216
# 9 frames :624
# 10 frames :397
# 11 frames :242
# 12 frames :264
# 13 frames :193
# 14 frames :274
# 15 frames :202
# 16 frames :172
# 17 frames :113
# 18 frames :111
# 19 frames :74
# 20 frames :80
# 21 frames :88
# 22 frames :68
# 23 frames :56
# 24 frames :55
# 25 frames :46
# 26 frames :43
# 27 frames :29
# 28 frames :45
# 29 frames :31
# 30 frames :27
# 31 frames :22
# 32 frames :16
# 33 frames :14
# 34 frames :24
# 35 frames :18
# 36 frames :13
# 37 frames :10
# 38 frames :8
# 39 frames :4
# 40 frames :8
# 41 frames :5
# 42 frames :7
# 43 frames :5
# 44 frames :10
# 45 frames :3
# 46 frames :4
# 47 frames :4
# 48 frames :4
# 49 frames :1
# 50 frames :1
# 51 frames :2
# 52 frames :2
# 53 frames :2
# 54 frames :1
# 55 frames :2
# 56 frames :1
# 57 frames :2
# 58 frames :3
# 59 frames :2
# 60 frames :1
# 61 frames :1
# 62 frames :1
# 66 frames :2
# 67 frames :1
# 68 frames :1
# 69 frames :1
# 72 frames :1
# 80 frames :1
# 90 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1896
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0