Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.56.28 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "2f0e76c3e24df5c207bf018cafb53486",
"name": "M1_20170604_1934_dsRNA_H4_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:56:48"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8088 (max 8089) in '8088'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.2701
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.76828e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1550 frames Cell Events: 70 mean_circ: 0.951053
Ran for 2050 frames Cell Events: 86 mean_circ: 0.952996
Ran for 3050 frames Cell Events: 125 mean_circ: 0.95172
Ran for 3550 frames Cell Events: 142 mean_circ: 0.951725
Ran for 4050 frames Cell Events: 159 mean_circ: 0.952062
Ran for 4550 frames Cell Events: 179 mean_circ: 0.951573
Ran for 5050 frames Cell Events: 194 mean_circ: 0.951371
Ran for 6050 frames Cell Events: 231 mean_circ: 0.950731
Ran for 6550 frames Cell Events: 252 mean_circ: 0.950975
Ran for 9050 frames Cell Events: 363 mean_circ: 0.950173
Ran for 9550 frames Cell Events: 379 mean_circ: 0.950109
Ran for 10050 frames Cell Events: 398 mean_circ: 0.950339
Ran for 10550 frames Cell Events: 416 mean_circ: 0.949964
Ran for 11050 frames Cell Events: 437 mean_circ: 0.948847
Ran for 12050 frames Cell Events: 478 mean_circ: 0.949427
Ran for 12550 frames Cell Events: 497 mean_circ: 0.949637
Ran for 13050 frames Cell Events: 511 mean_circ: 0.949705
Ran for 16050 frames Cell Events: 662 mean_circ: 0.948706
Ran for 17050 frames Cell Events: 710 mean_circ: 0.948626
Ran for 18050 frames Cell Events: 753 mean_circ: 0.94851
Ran for 20550 frames Cell Events: 876 mean_circ: 0.948459
Ran for 21550 frames Cell Events: 921 mean_circ: 0.94789
Ran for 22550 frames Cell Events: 967 mean_circ: 0.947721
Ran for 23050 frames Cell Events: 989 mean_circ: 0.947691
Ran for 23550 frames Cell Events: 1006 mean_circ: 0.947592
Ran for 24050 frames Cell Events: 1028 mean_circ: 0.94767
Ran for 24550 frames Cell Events: 1052 mean_circ: 0.947736
Ran for 25550 frames Cell Events: 1096 mean_circ: 0.947896
Ran for 26050 frames Cell Events: 1121 mean_circ: 0.947737
Ran for 26550 frames Cell Events: 1143 mean_circ: 0.948045
Ran for 27050 frames Cell Events: 1168 mean_circ: 0.947891
Ran for 30050 frames Cell Events: 1322 mean_circ: 0.947852
Ran for 30550 frames Cell Events: 1357 mean_circ: 0.94795
Ran for 34050 frames Cell Events: 1525 mean_circ: 0.947825
Ran for 36550 frames Cell Events: 1663 mean_circ: 0.94773
Ran for 38550 frames Cell Events: 1753 mean_circ: 0.947695
Ran for 39050 frames Cell Events: 1775 mean_circ: 0.947796
Ran for 40050 frames Cell Events: 1805 mean_circ: 0.947754
Ran for 41550 frames Cell Events: 1879 mean_circ: 0.947678
Ran for 42050 frames Cell Events: 1902 mean_circ: 0.94758
Ran for 43050 frames Cell Events: 1969 mean_circ: 0.947688
Ran for 43550 frames Cell Events: 1992 mean_circ: 0.947737
Ran for 44550 frames Cell Events: 2048 mean_circ: 0.947655
Ran for 46050 frames Cell Events: 2105 mean_circ: 0.947513
Ran for 46550 frames Cell Events: 2121 mean_circ: 0.947616
Ran for 47050 frames Cell Events: 2145 mean_circ: 0.94755
Ran for 47550 frames Cell Events: 2163 mean_circ: 0.947561
Ran for 48050 frames Cell Events: 2188 mean_circ: 0.947607
Ran for 48550 frames Cell Events: 2205 mean_circ: 0.947622
Ran for 49550 frames Cell Events: 2266 mean_circ: 0.947653
Ran for 50550 frames Cell Events: 2318 mean_circ: 0.947635
Ran for 51050 frames Cell Events: 2353 mean_circ: 0.947711
Ran for 51550 frames Cell Events: 2373 mean_circ: 0.94767
Ran for 52050 frames Cell Events: 2409 mean_circ: 0.947745
Ran for 53550 frames Cell Events: 2489 mean_circ: 0.947486
Ran for 55050 frames Cell Events: 2562 mean_circ: 0.947462
Ran for 56050 frames Cell Events: 2594 mean_circ: 0.947395
Ran for 57050 frames Cell Events: 2658 mean_circ: 0.947359
Ran for 57550 frames Cell Events: 2680 mean_circ: 0.947242
Ran for 58050 frames Cell Events: 2701 mean_circ: 0.94728
Ran for 59550 frames Cell Events: 2780 mean_circ: 0.947294
Ran for 61050 frames Cell Events: 2843 mean_circ: 0.947254
Ran for 61550 frames Cell Events: 2858 mean_circ: 0.947187
Ran for 63050 frames Cell Events: 2938 mean_circ: 0.947161
Ran for 64550 frames Cell Events: 3019 mean_circ: 0.947245
Ran for 65550 frames Cell Events: 3065 mean_circ: 0.947314
Ran for 66050 frames Cell Events: 3082 mean_circ: 0.947345
Ran for 67050 frames Cell Events: 3123 mean_circ: 0.94736
Ran for 67550 frames Cell Events: 3154 mean_circ: 0.94743
Ran for 68050 frames Cell Events: 3175 mean_circ: 0.947406
Ran for 68550 frames Cell Events: 3202 mean_circ: 0.947376
Ran for 69550 frames Cell Events: 3257 mean_circ: 0.947479
Ran for 70550 frames Cell Events: 3321 mean_circ: 0.94747
Ran for 71550 frames Cell Events: 3362 mean_circ: 0.9475
Ran for 72050 frames Cell Events: 3387 mean_circ: 0.947509
Ran for 73050 frames Cell Events: 3455 mean_circ: 0.947437
Ran for 74050 frames Cell Events: 3508 mean_circ: 0.947535
Ran for 76050 frames Cell Events: 3611 mean_circ: 0.94762
Ran for 77050 frames Cell Events: 3650 mean_circ: 0.947626
Ran for 77550 frames Cell Events: 3680 mean_circ: 0.947659
Ran for 79050 frames Cell Events: 3775 mean_circ: 0.947596
Ran for 80050 frames Cell Events: 3833 mean_circ: 0.947693
Ran for 80550 frames Cell Events: 3859 mean_circ: 0.947667
Ran for 81050 frames Cell Events: 3874 mean_circ: 0.947648
Ran for 81550 frames Cell Events: 3900 mean_circ: 0.947621
Ran for 82050 frames Cell Events: 3928 mean_circ: 0.947661
Ran for 83550 frames Cell Events: 4033 mean_circ: 0.947735
Ran for 85550 frames Cell Events: 4164 mean_circ: 0.947777
Ran for 86550 frames Cell Events: 4215 mean_circ: 0.947745
Ran for 87050 frames Cell Events: 4231 mean_circ: 0.947798
Ran for 87550 frames Cell Events: 4259 mean_circ: 0.947794
Ran for 88550 frames Cell Events: 4323 mean_circ: 0.947808
Ran for 89050 frames Cell Events: 4351 mean_circ: 0.947828
Ran for 89550 frames Cell Events: 4371 mean_circ: 0.947822
Ran for 90050 frames Cell Events: 4397 mean_circ: 0.947851
Ran for 90550 frames Cell Events: 4428 mean_circ: 0.947864
Ran for 91050 frames Cell Events: 4462 mean_circ: 0.947913
Ran for 91550 frames Cell Events: 4485 mean_circ: 0.947849
Ran for 93050 frames Cell Events: 4573 mean_circ: 0.947974
Ran for 94050 frames Cell Events: 4620 mean_circ: 0.948062
Ran for 94550 frames Cell Events: 4640 mean_circ: 0.94804
Ran for 95050 frames Cell Events: 4664 mean_circ: 0.947997
Ran for 96050 frames Cell Events: 4720 mean_circ: 0.948043
Ran for 98550 frames Cell Events: 4855 mean_circ: 0.948129
Ran for 99050 frames Cell Events: 4876 mean_circ: 0.948097
Ran for 100050 frames Cell Events: 4934 mean_circ: 0.948118
Ran for 100550 frames Cell Events: 4955 mean_circ: 0.948088
Ran for 101050 frames Cell Events: 4972 mean_circ: 0.948143
Ran for 102050 frames Cell Events: 5025 mean_circ: 0.948148
Ran for 103050 frames Cell Events: 5085 mean_circ: 0.948252
Ran for 103550 frames Cell Events: 5106 mean_circ: 0.948211
Ran for 104050 frames Cell Events: 5142 mean_circ: 0.948237
Ran for 104550 frames Cell Events: 5162 mean_circ: 0.948304
Ran for 105050 frames Cell Events: 5181 mean_circ: 0.948289
Ran for 105550 frames Cell Events: 5189 mean_circ: 0.948283
Ran for 107550 frames Cell Events: 5284 mean_circ: 0.948275
Ran for 108550 frames Cell Events: 5342 mean_circ: 0.948337
Ran for 111050 frames Cell Events: 5469 mean_circ: 0.948418
Ran for 111550 frames Cell Events: 5498 mean_circ: 0.948376
Ran for 112050 frames Cell Events: 5529 mean_circ: 0.948414
Ran for 114550 frames Cell Events: 5677 mean_circ: 0.948425
Ran for 115050 frames Cell Events: 5713 mean_circ: 0.948456
Ran for 118050 frames Cell Events: 5882 mean_circ: 0.948386
Ran for 119550 frames Cell Events: 5970 mean_circ: 0.948449
Ran for 120050 frames Cell Events: 6005 mean_circ: 0.948418
Ran for 120550 frames Cell Events: 6033 mean_circ: 0.948449
Ran for 121050 frames Cell Events: 6061 mean_circ: 0.948456
Ran for 121550 frames Cell Events: 6078 mean_circ: 0.948458
Ran for 123050 frames Cell Events: 6128 mean_circ: 0.948479
Ran for 123550 frames Cell Events: 6156 mean_circ: 0.948495
Ran for 125050 frames Cell Events: 6233 mean_circ: 0.948523
Ran for 128050 frames Cell Events: 6400 mean_circ: 0.948667
Ran for 129050 frames Cell Events: 6466 mean_circ: 0.948604
Ran for 129550 frames Cell Events: 6496 mean_circ: 0.948658
Ran for 130050 frames Cell Events: 6512 mean_circ: 0.948687
Ran for 135050 frames Cell Events: 6754 mean_circ: 0.948799
Ran for 135550 frames Cell Events: 6785 mean_circ: 0.948768
Ran for 138050 frames Cell Events: 6915 mean_circ: 0.94874
Ran for 139550 frames Cell Events: 6996 mean_circ: 0.948766
Ran for 140050 frames Cell Events: 7024 mean_circ: 0.948701
Ran for 140550 frames Cell Events: 7046 mean_circ: 0.948699
Ran for 141550 frames Cell Events: 7134 mean_circ: 0.948645
Ran for 143050 frames Cell Events: 7229 mean_circ: 0.948567
Ran for 143550 frames Cell Events: 7259 mean_circ: 0.94853
Ran for 144050 frames Cell Events: 7296 mean_circ: 0.948543
Ran for 145050 frames Cell Events: 7348 mean_circ: 0.948475
Ran for 146050 frames Cell Events: 7399 mean_circ: 0.948543
Ran for 146550 frames Cell Events: 7424 mean_circ: 0.94856
Ran for 147050 frames Cell Events: 7446 mean_circ: 0.948572
Ran for 148550 frames Cell Events: 7553 mean_circ: 0.94847
Ran for 150550 frames Cell Events: 7684 mean_circ: 0.948419
Ran for 151550 frames Cell Events: 7735 mean_circ: 0.948413
Ran for 152050 frames Cell Events: 7750 mean_circ: 0.948454
Ran for 153050 frames Cell Events: 7797 mean_circ: 0.948466
Ran for 153550 frames Cell Events: 7816 mean_circ: 0.948459
Ran for 155050 frames Cell Events: 7877 mean_circ: 0.948499
Ran for 155550 frames Cell Events: 7909 mean_circ: 0.948496
Ran for 156050 frames Cell Events: 7938 mean_circ: 0.948479
Ran for 156550 frames Cell Events: 7961 mean_circ: 0.94849
Ran for 158550 frames Cell Events: 8052 mean_circ: 0.948531
Ran for 159050 frames Cell Events: 8088 mean_circ: 0.948546



End this measurment..



Stopping time: 1.76836e+009
****************************************
Measurement finished

Mean Circularitiy: 0.948515
Mean Area: 589.386
Mean FL-1 intensity: 3499.07
Mean FL-2 intensity: 3806.44
Mean FL-3 intensity: 0

frames processed: 156602
Total Time: 79614.5ms
Number of Runs (new bg img) 0
Frames collected: 159100
Skipped frames: 152369
Rescued Frames: 5434813
Samples DAQ: 39775633
Samples written: 79549752
Samples per Channel: 39774876
FPS: 1998.38

Samples per second: 499593
Expected samples/s: 500000

Events: 8089
Events per second: 101.602
jumped back (PMT): 3622
#Frames read, frequency
# 0 frames :247653
# 1 frames :56365
# 2 frames :3208
# 3 frames :1183
# 4 frames :713
# 5 frames :374
# 6 frames :1165
# 7 frames :1420
# 8 frames :931
# 9 frames :636
# 10 frames :590
# 11 frames :456
# 12 frames :332
# 13 frames :264
# 14 frames :215
# 15 frames :209
# 16 frames :194
# 17 frames :143
# 18 frames :122
# 19 frames :113
# 20 frames :83
# 21 frames :100
# 22 frames :83
# 23 frames :56
# 24 frames :59
# 25 frames :37
# 26 frames :41
# 27 frames :34
# 28 frames :35
# 29 frames :26
# 30 frames :27
# 31 frames :30
# 32 frames :19
# 33 frames :16
# 34 frames :15
# 35 frames :9
# 36 frames :12
# 37 frames :12
# 38 frames :11
# 39 frames :12
# 40 frames :11
# 41 frames :5
# 42 frames :6
# 43 frames :7
# 44 frames :5
# 45 frames :7
# 46 frames :4
# 47 frames :3
# 48 frames :4
# 49 frames :3
# 50 frames :6
# 51 frames :2
# 52 frames :1
# 53 frames :1
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# 55 frames :1
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# 62 frames :1
# 65 frames :1
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# 69 frames :2
# 70 frames :1
# 75 frames :1
# 86 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.2701
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0