Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_18.04.41 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "cfc43504584c4396a18c5c4df97ea168",
"name": "M1_20170518_1810_i6_retest.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "16:05:09"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8125 (max 8126) in '8125'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 27.3734
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.94465e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 63 mean_circ: 0.941368
Ran for 1550 frames Cell Events: 88 mean_circ: 0.944194
Ran for 2050 frames Cell Events: 103 mean_circ: 0.944412
Ran for 2550 frames Cell Events: 128 mean_circ: 0.945138
Ran for 3050 frames Cell Events: 153 mean_circ: 0.944289
Ran for 4050 frames Cell Events: 203 mean_circ: 0.947095
Ran for 4550 frames Cell Events: 225 mean_circ: 0.945887
Ran for 5050 frames Cell Events: 250 mean_circ: 0.945918
Ran for 5550 frames Cell Events: 280 mean_circ: 0.946684
Ran for 6050 frames Cell Events: 319 mean_circ: 0.947774
Ran for 6550 frames Cell Events: 345 mean_circ: 0.94856
Ran for 7050 frames Cell Events: 372 mean_circ: 0.948634
Ran for 7550 frames Cell Events: 396 mean_circ: 0.94905
Ran for 10050 frames Cell Events: 538 mean_circ: 0.949061
Ran for 10550 frames Cell Events: 556 mean_circ: 0.948647
Ran for 13550 frames Cell Events: 735 mean_circ: 0.949061
Ran for 14550 frames Cell Events: 803 mean_circ: 0.948681
Ran for 15550 frames Cell Events: 849 mean_circ: 0.948857
Ran for 16050 frames Cell Events: 873 mean_circ: 0.949187
Ran for 16550 frames Cell Events: 889 mean_circ: 0.949009
Ran for 17050 frames Cell Events: 925 mean_circ: 0.949142
Ran for 18050 frames Cell Events: 965 mean_circ: 0.948592
Ran for 18550 frames Cell Events: 984 mean_circ: 0.948594
Ran for 19050 frames Cell Events: 1014 mean_circ: 0.948696
Ran for 20050 frames Cell Events: 1064 mean_circ: 0.948955
Ran for 24050 frames Cell Events: 1313 mean_circ: 0.949069
Ran for 24550 frames Cell Events: 1339 mean_circ: 0.949126
Ran for 25550 frames Cell Events: 1397 mean_circ: 0.948691
Ran for 27050 frames Cell Events: 1470 mean_circ: 0.949031
Ran for 28050 frames Cell Events: 1532 mean_circ: 0.948814
Ran for 28550 frames Cell Events: 1559 mean_circ: 0.948941
Ran for 29550 frames Cell Events: 1604 mean_circ: 0.948995
Ran for 30050 frames Cell Events: 1625 mean_circ: 0.948883
Ran for 30550 frames Cell Events: 1644 mean_circ: 0.948802
Ran for 31050 frames Cell Events: 1667 mean_circ: 0.948888
Ran for 32550 frames Cell Events: 1750 mean_circ: 0.949052
Ran for 33050 frames Cell Events: 1768 mean_circ: 0.949105
Ran for 34550 frames Cell Events: 1885 mean_circ: 0.949066
Ran for 35050 frames Cell Events: 1911 mean_circ: 0.949172
Ran for 35550 frames Cell Events: 1941 mean_circ: 0.949096
Ran for 37050 frames Cell Events: 2033 mean_circ: 0.948937
Ran for 37550 frames Cell Events: 2063 mean_circ: 0.948949
Ran for 38050 frames Cell Events: 2086 mean_circ: 0.948806
Ran for 40550 frames Cell Events: 2212 mean_circ: 0.948799
Ran for 41050 frames Cell Events: 2236 mean_circ: 0.948619
Ran for 41550 frames Cell Events: 2259 mean_circ: 0.948559
Ran for 42050 frames Cell Events: 2279 mean_circ: 0.948559
Ran for 42550 frames Cell Events: 2300 mean_circ: 0.948624
Ran for 43050 frames Cell Events: 2315 mean_circ: 0.948624
Ran for 44050 frames Cell Events: 2359 mean_circ: 0.948584
Ran for 45050 frames Cell Events: 2431 mean_circ: 0.948494
Ran for 45550 frames Cell Events: 2463 mean_circ: 0.948655
Ran for 46050 frames Cell Events: 2496 mean_circ: 0.948697
Ran for 46550 frames Cell Events: 2536 mean_circ: 0.948794
Ran for 48050 frames Cell Events: 2606 mean_circ: 0.948676
Ran for 49550 frames Cell Events: 2712 mean_circ: 0.948308
Ran for 50550 frames Cell Events: 2782 mean_circ: 0.948234
Ran for 51550 frames Cell Events: 2856 mean_circ: 0.948075
Ran for 52050 frames Cell Events: 2888 mean_circ: 0.94799
Ran for 52550 frames Cell Events: 2915 mean_circ: 0.947907
Ran for 53050 frames Cell Events: 2943 mean_circ: 0.947862
Ran for 53550 frames Cell Events: 2973 mean_circ: 0.947944
Ran for 54550 frames Cell Events: 3031 mean_circ: 0.948035
Ran for 56550 frames Cell Events: 3165 mean_circ: 0.947731
Ran for 57050 frames Cell Events: 3188 mean_circ: 0.947651
Ran for 57550 frames Cell Events: 3208 mean_circ: 0.947658
Ran for 58550 frames Cell Events: 3247 mean_circ: 0.947741
Ran for 59050 frames Cell Events: 3265 mean_circ: 0.947792
Ran for 60050 frames Cell Events: 3313 mean_circ: 0.947831
Ran for 61050 frames Cell Events: 3356 mean_circ: 0.94786
Ran for 61550 frames Cell Events: 3379 mean_circ: 0.947917
Ran for 62050 frames Cell Events: 3416 mean_circ: 0.947952
Ran for 63050 frames Cell Events: 3466 mean_circ: 0.947798
Ran for 64550 frames Cell Events: 3532 mean_circ: 0.947821
Ran for 65050 frames Cell Events: 3553 mean_circ: 0.947759
Ran for 66050 frames Cell Events: 3601 mean_circ: 0.947813
Ran for 66550 frames Cell Events: 3626 mean_circ: 0.947866
Ran for 68050 frames Cell Events: 3718 mean_circ: 0.947686
Ran for 69050 frames Cell Events: 3777 mean_circ: 0.947529
Ran for 69550 frames Cell Events: 3805 mean_circ: 0.947664
Ran for 70050 frames Cell Events: 3822 mean_circ: 0.947554
Ran for 71050 frames Cell Events: 3874 mean_circ: 0.947577
Ran for 71550 frames Cell Events: 3914 mean_circ: 0.947514
Ran for 72550 frames Cell Events: 3969 mean_circ: 0.947487
Ran for 73550 frames Cell Events: 4022 mean_circ: 0.94754
Ran for 75050 frames Cell Events: 4119 mean_circ: 0.947534
Ran for 79050 frames Cell Events: 4386 mean_circ: 0.94751
Ran for 79550 frames Cell Events: 4421 mean_circ: 0.947566
Ran for 80050 frames Cell Events: 4441 mean_circ: 0.947549
Ran for 81050 frames Cell Events: 4483 mean_circ: 0.947454
Ran for 81550 frames Cell Events: 4505 mean_circ: 0.947468
Ran for 82050 frames Cell Events: 4532 mean_circ: 0.947476
Ran for 83050 frames Cell Events: 4591 mean_circ: 0.947555
Ran for 83550 frames Cell Events: 4608 mean_circ: 0.947585
Ran for 84050 frames Cell Events: 4634 mean_circ: 0.947645
Ran for 84550 frames Cell Events: 4660 mean_circ: 0.947569
Ran for 87550 frames Cell Events: 4890 mean_circ: 0.947586
Ran for 89050 frames Cell Events: 4964 mean_circ: 0.947669
Ran for 90050 frames Cell Events: 5023 mean_circ: 0.947735
Ran for 90550 frames Cell Events: 5049 mean_circ: 0.947762
Ran for 91050 frames Cell Events: 5084 mean_circ: 0.947707
Ran for 91550 frames Cell Events: 5101 mean_circ: 0.947778
Ran for 92050 frames Cell Events: 5125 mean_circ: 0.947861
Ran for 92550 frames Cell Events: 5150 mean_circ: 0.947919
Ran for 93050 frames Cell Events: 5177 mean_circ: 0.947904
Ran for 94550 frames Cell Events: 5270 mean_circ: 0.947888
Ran for 95550 frames Cell Events: 5335 mean_circ: 0.947897
Ran for 96050 frames Cell Events: 5366 mean_circ: 0.947957
Ran for 98050 frames Cell Events: 5498 mean_circ: 0.947968
Ran for 98550 frames Cell Events: 5529 mean_circ: 0.94797
Ran for 99050 frames Cell Events: 5566 mean_circ: 0.947928
Ran for 99550 frames Cell Events: 5603 mean_circ: 0.947946
Ran for 100050 frames Cell Events: 5624 mean_circ: 0.948028
Ran for 101550 frames Cell Events: 5698 mean_circ: 0.948
Ran for 103550 frames Cell Events: 5823 mean_circ: 0.948016
Ran for 104550 frames Cell Events: 5897 mean_circ: 0.947942
Ran for 107050 frames Cell Events: 6016 mean_circ: 0.947984
Ran for 108050 frames Cell Events: 6056 mean_circ: 0.947917
Ran for 108550 frames Cell Events: 6078 mean_circ: 0.947931
Ran for 109550 frames Cell Events: 6141 mean_circ: 0.947823
Ran for 110050 frames Cell Events: 6174 mean_circ: 0.947898
Ran for 111550 frames Cell Events: 6268 mean_circ: 0.947946
Ran for 112550 frames Cell Events: 6313 mean_circ: 0.947969
Ran for 113050 frames Cell Events: 6350 mean_circ: 0.94804
Ran for 113550 frames Cell Events: 6370 mean_circ: 0.94801
Ran for 114050 frames Cell Events: 6403 mean_circ: 0.94801
Ran for 115050 frames Cell Events: 6458 mean_circ: 0.947885
Ran for 116050 frames Cell Events: 6533 mean_circ: 0.947889
Ran for 117050 frames Cell Events: 6570 mean_circ: 0.947804
Ran for 117550 frames Cell Events: 6593 mean_circ: 0.947801
Ran for 118050 frames Cell Events: 6611 mean_circ: 0.947775
Ran for 118550 frames Cell Events: 6639 mean_circ: 0.947764
Ran for 119050 frames Cell Events: 6667 mean_circ: 0.947825
Ran for 119550 frames Cell Events: 6693 mean_circ: 0.94786
Ran for 120550 frames Cell Events: 6744 mean_circ: 0.947888
Ran for 122550 frames Cell Events: 6894 mean_circ: 0.947986
Ran for 123050 frames Cell Events: 6920 mean_circ: 0.948023
Ran for 123550 frames Cell Events: 6934 mean_circ: 0.948032
Ran for 124550 frames Cell Events: 6981 mean_circ: 0.947989
Ran for 125050 frames Cell Events: 7011 mean_circ: 0.947951
Ran for 125550 frames Cell Events: 7037 mean_circ: 0.947968
Ran for 126050 frames Cell Events: 7068 mean_circ: 0.947905
Ran for 126550 frames Cell Events: 7093 mean_circ: 0.947893
Ran for 127550 frames Cell Events: 7144 mean_circ: 0.94793
Ran for 128050 frames Cell Events: 7164 mean_circ: 0.947959
Ran for 128550 frames Cell Events: 7190 mean_circ: 0.947973
Ran for 129550 frames Cell Events: 7244 mean_circ: 0.947975
Ran for 130050 frames Cell Events: 7275 mean_circ: 0.948028
Ran for 131050 frames Cell Events: 7325 mean_circ: 0.948064
Ran for 131550 frames Cell Events: 7339 mean_circ: 0.948073
Ran for 132550 frames Cell Events: 7397 mean_circ: 0.948147
Ran for 133050 frames Cell Events: 7424 mean_circ: 0.948185
Ran for 133550 frames Cell Events: 7457 mean_circ: 0.948221
Ran for 135550 frames Cell Events: 7582 mean_circ: 0.94811
Ran for 136050 frames Cell Events: 7616 mean_circ: 0.948135
Ran for 137050 frames Cell Events: 7678 mean_circ: 0.948139
Ran for 137550 frames Cell Events: 7716 mean_circ: 0.948124
Ran for 140550 frames Cell Events: 7888 mean_circ: 0.948065
Ran for 142050 frames Cell Events: 7960 mean_circ: 0.948039
Ran for 143050 frames Cell Events: 8008 mean_circ: 0.947992



End this measurment..



Stopping time: 2.94537e+008
****************************************
Measurement finished

Mean Circularitiy: 0.947932
Mean Area: 632.18
Mean FL-1 intensity: 3574.36
Mean FL-2 intensity: 5285.35
Mean FL-3 intensity: 0

frames processed: 144127
Total Time: 72495.2ms
Number of Runs (new bg img) 0
Frames collected: 144849
Skipped frames: 68397
Rescued Frames: 3865726
Samples DAQ: 36212337
Samples written: 72424592
Samples per Channel: 36212296
FPS: 1998.05

Samples per second: 499513
Expected samples/s: 500000

Events: 8126
Events per second: 112.09
jumped back (PMT): 3261
#Frames read, frequency
# 0 frames :219047
# 1 frames :59017
# 2 frames :2722
# 3 frames :995
# 4 frames :479
# 5 frames :179
# 6 frames :1793
# 7 frames :1021
# 8 frames :913
# 9 frames :561
# 10 frames :753
# 11 frames :255
# 12 frames :261
# 13 frames :142
# 14 frames :264
# 15 frames :225
# 16 frames :194
# 17 frames :133
# 18 frames :85
# 19 frames :67
# 20 frames :74
# 21 frames :68
# 22 frames :64
# 23 frames :56
# 24 frames :41
# 25 frames :25
# 26 frames :35
# 27 frames :19
# 28 frames :26
# 29 frames :18
# 30 frames :9
# 31 frames :21
# 32 frames :14
# 33 frames :11
# 34 frames :2
# 35 frames :8
# 36 frames :7
# 37 frames :4
# 38 frames :4
# 39 frames :5
# 40 frames :3
# 43 frames :1
# 44 frames :2
# 46 frames :1
# 50 frames :1
# 53 frames :2
# 60 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 27.3734
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0