Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_18.21.08 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "917e31766e0151a8859c2d07d0a42014",
"name": "M1_20170519_1327_dsRNA_K2_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "16:21:33"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8218 (max 8219) in '8218'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 27.3168
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.63898e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 16 mean_circ: 0.955515
Ran for 1050 frames Cell Events: 33 mean_circ: 0.960475
Ran for 2550 frames Cell Events: 98 mean_circ: 0.960383
Ran for 3050 frames Cell Events: 115 mean_circ: 0.960037
Ran for 5050 frames Cell Events: 201 mean_circ: 0.957759
Ran for 5550 frames Cell Events: 220 mean_circ: 0.957467
Ran for 6050 frames Cell Events: 247 mean_circ: 0.956546
Ran for 6550 frames Cell Events: 263 mean_circ: 0.955703
Ran for 7050 frames Cell Events: 296 mean_circ: 0.955367
Ran for 8050 frames Cell Events: 340 mean_circ: 0.955041
Ran for 8550 frames Cell Events: 370 mean_circ: 0.954792
Ran for 10050 frames Cell Events: 456 mean_circ: 0.954238
Ran for 11550 frames Cell Events: 544 mean_circ: 0.954222
Ran for 12550 frames Cell Events: 591 mean_circ: 0.954303
Ran for 13550 frames Cell Events: 632 mean_circ: 0.953795
Ran for 14050 frames Cell Events: 644 mean_circ: 0.953849
Ran for 14550 frames Cell Events: 667 mean_circ: 0.954187
Ran for 15050 frames Cell Events: 691 mean_circ: 0.954186
Ran for 16050 frames Cell Events: 742 mean_circ: 0.953727
Ran for 17050 frames Cell Events: 798 mean_circ: 0.953997
Ran for 17550 frames Cell Events: 821 mean_circ: 0.954017
Ran for 18050 frames Cell Events: 839 mean_circ: 0.953865
Ran for 18550 frames Cell Events: 871 mean_circ: 0.953803
Ran for 19550 frames Cell Events: 932 mean_circ: 0.953263
Ran for 20550 frames Cell Events: 982 mean_circ: 0.953365
Ran for 21050 frames Cell Events: 1012 mean_circ: 0.953237
Ran for 21550 frames Cell Events: 1030 mean_circ: 0.953247
Ran for 22550 frames Cell Events: 1084 mean_circ: 0.953141
Ran for 23550 frames Cell Events: 1138 mean_circ: 0.953177
Ran for 24050 frames Cell Events: 1164 mean_circ: 0.952891
Ran for 24550 frames Cell Events: 1185 mean_circ: 0.952538
Ran for 25050 frames Cell Events: 1219 mean_circ: 0.952588
Ran for 25550 frames Cell Events: 1241 mean_circ: 0.952798
Ran for 26050 frames Cell Events: 1270 mean_circ: 0.952915
Ran for 26550 frames Cell Events: 1288 mean_circ: 0.953048
Ran for 32050 frames Cell Events: 1598 mean_circ: 0.952668
Ran for 32550 frames Cell Events: 1623 mean_circ: 0.952538
Ran for 33050 frames Cell Events: 1647 mean_circ: 0.952261
Ran for 35050 frames Cell Events: 1753 mean_circ: 0.952165
Ran for 35550 frames Cell Events: 1779 mean_circ: 0.952258
Ran for 36050 frames Cell Events: 1798 mean_circ: 0.95242
Ran for 37050 frames Cell Events: 1854 mean_circ: 0.952524
Ran for 39050 frames Cell Events: 1941 mean_circ: 0.952533
Ran for 39550 frames Cell Events: 1968 mean_circ: 0.952647
Ran for 40550 frames Cell Events: 2004 mean_circ: 0.952785
Ran for 41050 frames Cell Events: 2036 mean_circ: 0.952846
Ran for 41550 frames Cell Events: 2072 mean_circ: 0.952909
Ran for 42050 frames Cell Events: 2089 mean_circ: 0.952848
Ran for 43050 frames Cell Events: 2116 mean_circ: 0.952892
Ran for 43550 frames Cell Events: 2138 mean_circ: 0.952951
Ran for 44050 frames Cell Events: 2160 mean_circ: 0.952974
Ran for 44550 frames Cell Events: 2184 mean_circ: 0.95293
Ran for 45550 frames Cell Events: 2239 mean_circ: 0.952952
Ran for 46050 frames Cell Events: 2259 mean_circ: 0.953005
Ran for 46550 frames Cell Events: 2283 mean_circ: 0.952933
Ran for 47050 frames Cell Events: 2297 mean_circ: 0.952963
Ran for 48050 frames Cell Events: 2358 mean_circ: 0.953059
Ran for 49050 frames Cell Events: 2421 mean_circ: 0.953103
Ran for 49550 frames Cell Events: 2442 mean_circ: 0.952973
Ran for 51050 frames Cell Events: 2540 mean_circ: 0.952936
Ran for 51550 frames Cell Events: 2551 mean_circ: 0.952991
Ran for 52050 frames Cell Events: 2562 mean_circ: 0.952946
Ran for 52550 frames Cell Events: 2590 mean_circ: 0.953004
Ran for 53050 frames Cell Events: 2604 mean_circ: 0.952918
Ran for 55550 frames Cell Events: 2757 mean_circ: 0.952952
Ran for 57050 frames Cell Events: 2826 mean_circ: 0.952863
Ran for 57550 frames Cell Events: 2842 mean_circ: 0.952923
Ran for 58550 frames Cell Events: 2876 mean_circ: 0.952991
Ran for 59050 frames Cell Events: 2895 mean_circ: 0.953004
Ran for 60050 frames Cell Events: 2935 mean_circ: 0.953032
Ran for 60550 frames Cell Events: 2961 mean_circ: 0.953074
Ran for 61550 frames Cell Events: 3009 mean_circ: 0.953074
Ran for 62050 frames Cell Events: 3031 mean_circ: 0.953119
Ran for 62550 frames Cell Events: 3054 mean_circ: 0.953097
Ran for 64550 frames Cell Events: 3148 mean_circ: 0.952998
Ran for 65550 frames Cell Events: 3217 mean_circ: 0.952993
Ran for 66550 frames Cell Events: 3281 mean_circ: 0.95291
Ran for 67050 frames Cell Events: 3302 mean_circ: 0.952977
Ran for 67550 frames Cell Events: 3331 mean_circ: 0.952844
Ran for 68050 frames Cell Events: 3349 mean_circ: 0.952864
Ran for 69050 frames Cell Events: 3407 mean_circ: 0.952857
Ran for 70550 frames Cell Events: 3485 mean_circ: 0.952788
Ran for 72050 frames Cell Events: 3566 mean_circ: 0.952843
Ran for 72550 frames Cell Events: 3587 mean_circ: 0.952852
Ran for 73550 frames Cell Events: 3631 mean_circ: 0.952884
Ran for 74050 frames Cell Events: 3667 mean_circ: 0.952909
Ran for 75050 frames Cell Events: 3723 mean_circ: 0.952948
Ran for 75550 frames Cell Events: 3757 mean_circ: 0.952861
Ran for 76550 frames Cell Events: 3800 mean_circ: 0.95296
Ran for 77050 frames Cell Events: 3826 mean_circ: 0.953016
Ran for 77550 frames Cell Events: 3848 mean_circ: 0.953015
Ran for 78050 frames Cell Events: 3873 mean_circ: 0.953001
Ran for 78550 frames Cell Events: 3897 mean_circ: 0.952898
Ran for 79050 frames Cell Events: 3916 mean_circ: 0.952899
Ran for 81550 frames Cell Events: 4038 mean_circ: 0.952735
Ran for 82550 frames Cell Events: 4092 mean_circ: 0.952707
Ran for 83550 frames Cell Events: 4143 mean_circ: 0.952689
Ran for 84050 frames Cell Events: 4168 mean_circ: 0.952723
Ran for 84550 frames Cell Events: 4192 mean_circ: 0.952742
Ran for 85050 frames Cell Events: 4206 mean_circ: 0.952776
Ran for 86050 frames Cell Events: 4267 mean_circ: 0.952616
Ran for 88050 frames Cell Events: 4396 mean_circ: 0.952627
Ran for 88550 frames Cell Events: 4410 mean_circ: 0.952631
Ran for 89050 frames Cell Events: 4431 mean_circ: 0.952654
Ran for 89550 frames Cell Events: 4453 mean_circ: 0.952698
Ran for 90050 frames Cell Events: 4476 mean_circ: 0.95255
Ran for 90550 frames Cell Events: 4497 mean_circ: 0.952507
Ran for 92550 frames Cell Events: 4590 mean_circ: 0.952498
Ran for 93550 frames Cell Events: 4632 mean_circ: 0.952524
Ran for 94550 frames Cell Events: 4682 mean_circ: 0.952438
Ran for 95550 frames Cell Events: 4724 mean_circ: 0.952431
Ran for 96050 frames Cell Events: 4753 mean_circ: 0.952468
Ran for 97050 frames Cell Events: 4801 mean_circ: 0.952508
Ran for 97550 frames Cell Events: 4826 mean_circ: 0.952469
Ran for 99050 frames Cell Events: 4921 mean_circ: 0.952425
Ran for 100550 frames Cell Events: 4996 mean_circ: 0.952512
Ran for 101050 frames Cell Events: 5019 mean_circ: 0.952541
Ran for 102050 frames Cell Events: 5058 mean_circ: 0.952537
Ran for 103550 frames Cell Events: 5141 mean_circ: 0.952614
Ran for 104550 frames Cell Events: 5200 mean_circ: 0.952615
Ran for 107050 frames Cell Events: 5297 mean_circ: 0.952687
Ran for 108550 frames Cell Events: 5374 mean_circ: 0.952668
Ran for 109050 frames Cell Events: 5394 mean_circ: 0.952697
Ran for 110550 frames Cell Events: 5473 mean_circ: 0.952701
Ran for 111050 frames Cell Events: 5486 mean_circ: 0.952709
Ran for 111550 frames Cell Events: 5507 mean_circ: 0.952696
Ran for 112550 frames Cell Events: 5549 mean_circ: 0.952691
Ran for 113050 frames Cell Events: 5582 mean_circ: 0.952714
Ran for 114550 frames Cell Events: 5668 mean_circ: 0.952687
Ran for 115050 frames Cell Events: 5689 mean_circ: 0.952692
Ran for 116550 frames Cell Events: 5798 mean_circ: 0.952683
Ran for 117050 frames Cell Events: 5816 mean_circ: 0.952741
Ran for 117550 frames Cell Events: 5834 mean_circ: 0.95276
Ran for 118050 frames Cell Events: 5853 mean_circ: 0.952761
Ran for 122050 frames Cell Events: 6090 mean_circ: 0.952834
Ran for 122550 frames Cell Events: 6119 mean_circ: 0.952846
Ran for 123550 frames Cell Events: 6165 mean_circ: 0.952852
Ran for 124050 frames Cell Events: 6193 mean_circ: 0.95286
Ran for 124550 frames Cell Events: 6218 mean_circ: 0.952835
Ran for 125550 frames Cell Events: 6260 mean_circ: 0.952842
Ran for 126050 frames Cell Events: 6281 mean_circ: 0.952868
Ran for 126550 frames Cell Events: 6304 mean_circ: 0.952875
Ran for 127050 frames Cell Events: 6325 mean_circ: 0.952903
Ran for 128050 frames Cell Events: 6388 mean_circ: 0.952904
Ran for 128550 frames Cell Events: 6411 mean_circ: 0.952895
Ran for 129050 frames Cell Events: 6436 mean_circ: 0.95291
Ran for 129550 frames Cell Events: 6455 mean_circ: 0.95291
Ran for 130050 frames Cell Events: 6486 mean_circ: 0.952909
Ran for 131050 frames Cell Events: 6528 mean_circ: 0.952893
Ran for 133050 frames Cell Events: 6609 mean_circ: 0.952896
Ran for 134050 frames Cell Events: 6645 mean_circ: 0.952905
Ran for 135550 frames Cell Events: 6723 mean_circ: 0.952961
Ran for 136050 frames Cell Events: 6750 mean_circ: 0.952945
Ran for 137550 frames Cell Events: 6835 mean_circ: 0.95295
Ran for 138050 frames Cell Events: 6856 mean_circ: 0.952909
Ran for 138550 frames Cell Events: 6881 mean_circ: 0.952893
Ran for 139050 frames Cell Events: 6915 mean_circ: 0.952838
Ran for 141050 frames Cell Events: 7016 mean_circ: 0.952858
Ran for 141550 frames Cell Events: 7036 mean_circ: 0.952892
Ran for 142050 frames Cell Events: 7056 mean_circ: 0.952909
Ran for 143050 frames Cell Events: 7114 mean_circ: 0.95293
Ran for 144550 frames Cell Events: 7188 mean_circ: 0.952955
Ran for 147050 frames Cell Events: 7342 mean_circ: 0.952974
Ran for 147550 frames Cell Events: 7373 mean_circ: 0.953013
Ran for 149050 frames Cell Events: 7450 mean_circ: 0.95312
Ran for 149550 frames Cell Events: 7470 mean_circ: 0.953143
Ran for 153050 frames Cell Events: 7719 mean_circ: 0.953258
Ran for 155550 frames Cell Events: 7867 mean_circ: 0.953214
Ran for 156550 frames Cell Events: 7906 mean_circ: 0.953279
Ran for 157050 frames Cell Events: 7934 mean_circ: 0.953304
Ran for 157550 frames Cell Events: 7953 mean_circ: 0.9533
Ran for 158550 frames Cell Events: 8000 mean_circ: 0.953289
Ran for 160050 frames Cell Events: 8077 mean_circ: 0.953321
Ran for 160550 frames Cell Events: 8105 mean_circ: 0.953304
Ran for 161550 frames Cell Events: 8167 mean_circ: 0.953327
Ran for 162050 frames Cell Events: 8191 mean_circ: 0.953311



End this measurment..



Stopping time: 3.63979e+008
****************************************
Measurement finished

Mean Circularitiy: 0.953327
Mean Area: 553.746
Mean FL-1 intensity: 2416.81
Mean FL-2 intensity: 4009.04
Mean FL-3 intensity: 0

frames processed: 161954
Total Time: 81294.1ms
Number of Runs (new bg img) 0
Frames collected: 162434
Skipped frames: 6239
Rescued Frames: 432247
Samples DAQ: 40615651
Samples written: 81216772
Samples per Channel: 40608386
FPS: 1998.1

Samples per second: 499524
Expected samples/s: 500000

Events: 8219
Events per second: 101.102
jumped back (PMT): 3459
#Frames read, frequency
# 0 frames :245748
# 1 frames :65883
# 2 frames :3342
# 3 frames :1106
# 4 frames :666
# 5 frames :295
# 6 frames :1272
# 7 frames :1578
# 8 frames :950
# 9 frames :578
# 10 frames :694
# 11 frames :434
# 12 frames :375
# 13 frames :226
# 14 frames :215
# 15 frames :258
# 16 frames :216
# 17 frames :168
# 18 frames :125
# 19 frames :78
# 20 frames :69
# 21 frames :90
# 22 frames :56
# 23 frames :51
# 24 frames :39
# 25 frames :38
# 26 frames :31
# 27 frames :25
# 28 frames :24
# 29 frames :15
# 30 frames :22
# 31 frames :15
# 32 frames :9
# 33 frames :9
# 34 frames :8
# 35 frames :9
# 36 frames :7
# 37 frames :8
# 38 frames :4
# 39 frames :4
# 40 frames :2
# 41 frames :2
# 42 frames :1
# 43 frames :3
# 44 frames :3
# 45 frames :5
# 46 frames :2
# 47 frames :1
# 48 frames :3
# 49 frames :1
# 50 frames :1
# 51 frames :4
# 53 frames :1
# 54 frames :1
# 55 frames :1
# 56 frames :1
# 58 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 27.3168
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0